Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_ssc0000204.1

Orthogroup ID

OG0003605

Physical Location & Seq

Genomic Coordinates Forward (+)

111777 .. 130668

Gene Structure 18892 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6906 bp

atgactcaggagggagctcctccaccgatgagtcactcaaattccgccgcaaaatctactttgaggatgccgttctcggaccaaagaagcagaggagcagtggtgaacagaggttttgccaacagtgtagcagctacatggcagcgtgacatggcaccaagctcaatagacttggcggaacttgtgacacttcactttggatgcaagatatctgaggagaagttttcggtgctttggacaaaatcagatgtttctgtgacatttgggatggaacagaaaaatgtgcacttgtctttctcctatgattgtgttaattacaagcttgagatcccctctgacactatttgccggattgagctgcattgtccacgtggtcagtttaaaaagtttctactcatccagttacatggtgcccctcggatttataagaaagatataactttggctcctgacaaccgctgggttcgagaagttgatttcactccatcatactgcatcgggcaatcctcttctttatgtttggagcttccatttaggtgtgcacttccagtccttcgcaagagttttgttcactataaggagaataaacgacagtttgagctgaagagaggtcacactttttccggcaattcagctcttgttcctattctggctccacccataggcattgatttgccatacagaatcttgttcaagataaattcattggttcaacacggttctattccctggcaagctcttgatgtcaatttttataatctagtggatcctactagaataagaattgaatatatacagtatgccctggacaaactgtttaactccaaggagtgctgttatgaacctggaaggtggctttttaacatgtacaaggcatgcaagcggattcctgagtcaccagcaatatctttagatgaaggccttgtgtatatacacagggttcaggttacaccatctaaagtatacttctgtggtccagaggtaaatctctccaatcgtgttttacgaaattatcctgaagatatcgacaattttcttcgggtttcttttgttgatgaggacttgggaaagatgcggtctgtagatttgtgtctgcggacaagtactgcagaagaggaaaggagaaccagtgtttatgaaaggatactttctactctaagggatggcatagtgattggtgataagaagtttgattttctagcattttcgtcgagtcagttgcgagagaattctgcatggatgtttgcttccagaaggggccttactgcacaaggaattagagactggatgggtgattttagtcgcattaaaaatgtggctaaatatgcagccaggcttggtcagtctttcggctcttccagggagacttttactgttggctcagatgaaattgaagtcattccggatgtagaaattgaaagaggcggaaccaaatattgcttctctgacggaattgggaagatatctgctgaatttgctgaaagagtggcaagaaaatgtgggaaaagttctactccatctgcctttcaaatccgctacggtggctacaaaggtgttgtggcaatcgatccaacatcgtcaaagaagctgtcattgagaaagagcatgtggaagttcgaatcacgcaacacaaagttagatgtcctggcatggagcaagtaccagccttgttttctcaatcgtcaactgattacccttttgtccacccttggagttaaggatcatatttttgaggagaagcagaaacaagcaatgaatcagctggaagggattctaactgatccattgaaagcacagaaggcactgaaattgatgtttaaaggagaggcaaccagcatgttaaatgaaatgctgaagtgttataagccaaatgcagagccgtttctctcattgatgctacaatcattatgtgcatcaaaacttgcagaactgcgaagcaaaacacgtatttttgttccagatggaagatctctgatgggatgtctagatgaaaccgggacattggaatatggtcaggtatttgtgcaatgttctcaccatgctatcttcactggcaaaacaacttcaactgcaaggcgaggcaattttactgttgtgggtaaagtagtggttgctaaaaacccgtgtttacaccctggagatgtgcgtgttcttatggctgtggatgtgccggcgttacaccacatggtggattgtgttgtgttccccaaaaaaggaaagaggcctcatcctaatgaatgctcaggcagcgatttagatggagatgattactttgttagctgggatggtgacctgattccaccgctgcaaactgaacccatgaactataccccagcaccaactgtggaactggatcataatgttacaatggaggaagttgaggagtcttttaccaactatataatgaattgcagcttgggcatcatttcaaatgcacatcttgtctttgcagacagaaaaccaaagaaggctatgagtgatgaatgtattaaactcgccaagctccactcccatgctgttgattctccaaaaaccggtgtactagtgaaagtgccgcatagactgcgtgtccaagaattcccagatttcatggaaaagagtgacaggcagacctacgagtccaagcgtgtgattgggaagcttttccgacaggtaaaagatattgaccttggatcacattcacaccctgttaagtccttcactagggaagtggctaagaggtattatgatcctgacatggaggtagatggattcaaagattacattaatgatgccatcagtcacaaaggcgattatgactacaagctgggaaacctgatggactattatggtatcaaaactgaagctgaaatactgagtggaaacatcaccaatgtatcaaagttcttcaacaaaagaaaggacttggagtcgatcaactacgctgtgaagtcattgaagaatgaggcaatgacactgtttatcgagagtccaaatagtgatgatccatcagatttcggaactgatatcggtaatgtgtatgcagcaaaagcatcagcatggtaccatgttacatatcatcatcgttactggggttgctacaacaaggcggtaccggtgaaagttgattgcctcccgaaaggtaggccctcgcctcatatccaaatcctcactctttcggtctttcccgactgcctccacacctttcctctctcccaaacccccattatccctgggttctcttccaaggttcgagattctagggtttcggtgacggtcgttccagccagcctcgacgcagtcaccgggattaggcctggcggagctatcgagagcgataagctaccgtccgatgtgaggaagcgcaccatggacgttgtcgacgcctgcggagggagagtgacgattggttcggggtataagaggtttaaggtacaagaagacatggggttaggggagcagagaattggaattgatggagtgacatgcttgacctggcttggggtatctaggtacctagcaacaggttgtgcggatgggaaggctaccgacatgctgcgcgccatgctgccgagcccttcacacgacggtgaatctgctgaaaaaattaaggtatttttggagaaacatacaggagaaggaagtgtttatgctcttaaattacggccggccaaagggggaagattctatgcttttatacagtttgagtccaccagtcaggctgagcttatcacctctttggctaaagttgggctgtggtatgggagatcatatttgaaagcttatccagcggaccatgatattgtaccaaagccaagaactgtaatgcatagcttgcaaaatttagtattgcatgttggctgccaggttgcaaatgataagttgtctgttttttggagaacggctgaagtttcagtggactttgggactggaacgcggaaattccaatttcggctgttatatcatcagaagttatatagacttcagctttcgtatgagaacatctggcaaattgagttgcatcgtccacgtggtcgcactgcaaaatatcttgtcattcagttatatggagctcctcgcatctaccaacaagaagaagcatgttcttctggaaatgtatatgatgaccctcttatgaactactttagggatgtccctgatgaccaatgggttcggaccactgatttcactccacgaagctgtattggggagtcatcttcattatgtttagagcttccttctggtcgccaacttcctgattttgaagaaaattttgcttattataaggagagtgatgggcaatttatcttggagaatggctcaactttctcttgcaaccgagatctggtccctattgttggttctcctgcaggggttgatttgccatatgatatcttgtttaaaataaactcgctggttcaaatgggttgtctgtcagggccgacacttgataataggttttatagactggtggatccacgaagaataactcgttcctgtatagagtatgccctagataaattatctaatttgaaaaagagctgttatgaacctgcggtgtggcttaatgaggagtaccggaagtacctgactacaaagaaacctccaaagccggcacttgtctccttagattctggtttggtatatgtacggagggttcaaataacaccatctaaagtgtatttttctggtcctgaggttaatgtctcaaatcgcatactacgccagtattccagtgatatcgataactttcttcgtgtttcatttgttgatgaggagatggaaaatctctattcaacagatttgtccccccgtacatctactagaaaaactcgcatctacaacagagttctttctattctcagaaacggcatagtaattggagaaaagaaatttgagtttcttgcattctcatcaagtcaattgcaagagaattcattgtggatgttttcttcaacctctaatgttagtgctgataagataagagcagggatgggggattttcgcaagataagaaatgtggcaaagtatgctgccagattgggtcaatcttttggctcttctactgaaactctaagtgttgccaggaatgagattgaagttattcctgatgtaacagttaattggggtggagtcaaatatgtgttctccgatgggattgggaaaatatcagctgattttgcaaagagagtagcctccaaatgtggctataaagcgttcacaccatctgcatttcaaattcgatatggtggttacaaaggtgttgtagctgttgatccaacctcgtcaatgaaattatcactgagaaagagcatgtctaagtatgattctgaaaacacaaagctagatgttctagcatgtagcaagtttcagccttgttttttgaatcgtcagttgataactcttctgtctacacttggagttcaagatcatgttttcttgaaaaagcaaagagcagctgtacaacaacttaatgccatattgactgacccattaaaagcaggggaggctcttgatttgatgtctgcgagtgagtgtacaaacatcctcaaggaactgctgatgtgtggttataaacctgatgttgaaccattcctctcaatgatgctccaaacatttcgggcatcaaagttgttggaattgaggaccaaaacaagaatctttatcccagatggaagagcaatgatgggatgtctagacgaaacaagaaccttggaatatgggcaggtgtttgtgcaaatctgtggaaacagacgaaggcagttatttgatgattcattcatgcatagtggaagctcatcaggcaaaacagttattgaaggaaaggtggttgttgcaaagaacccatgtttacaccctggtgatgtgcgtgtcttaagggctgtgaatgtgccggagttacaacatatggtggactgtgtggtatttccacaaaaaggaccaaggcctcatccaaatgaatgttctggaagtgatcttgatggagatatttactttgtttgctgggaccccgagttgattcctcgcaagcaaatccaacctatggattataccccagcaccaactatagagatggatcatgatgtcacaattgaggaagtagaagagcactttgtgaattacatactcaatgatagtttggggagaattgccaatgcccacactgtttttgctgacagagagcatgataaagcaatgagccttccgtgcatagagctcgcgaagctacattcaattgctgtagactttccaaaaactggcgtgccagctgtgagaccaaaggaactgtatgtcaaggaatatccagatttcatggagaagaccgacaaaagcacctatctctcaagcaatgtgatcggacagctttttcgtgaggtgaagaacattgcaccccatgacagctgtattacatccttcactcatcaagtggcgacaaaatcctatgaccctgacatggaggttgatggctttgaggattaccttgaagatgctttctattacaaaggcaattatgattacaaattgggaaacctgttggattactatgggatcaagacagaggccgaaatattgagtgggggtattatgagaatgtcaaaatctttcaccaaggagcgggatgcagacgctataaacagggctgtgaggtcattgaggaaggaagctaggacctggttcgatgagaaaggaactggaatagattctggagttgatggtgtctatgcaaaagcttcagcttggtaccatgtcacatatcatcccaattactttggtttgtacaatgaaggaatgaaacgtgaccattttatcagcttcccgtggtgtgtgtatgacaagctagttcacataaaaaaggacaaagtaaagcaagctcggcgcagatcatctctagaaagtcagttcaggtacggcttgcatttgttctga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009615 GO:0009719 GO:0009725 GO:0009751 GO:0009892 GO:0009987 GO:0010025 GO:0010033 GO:0010166 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0042493 GO:0043170 GO:0043207 GO:0043331 GO:0044237 GO:0044238 GO:0046483 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060147 GO:0060148 GO:0060255 GO:0060968 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901568 GO:1901570 GO:1901576 GO:1901698 GO:1901699 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RdRP

Protein Analysis

2301

Amino Acids

260.26

Weight (kDa)

8.65

Isoelectric Point (pI)

40.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH_RDR2	PF24823	64 - 199	2.4e-30	RDR2 PH-like domain
RdRP_helical	PF26252	217 - 297	3.2e-09	RNA dependent RNA polymerase, helical domain
RdRP	PF05183	316 - 885	8.3e-195	RNA dependent RNA polymerase
RdRP_head	PF26253	909 - 1029	8.1e-33	RdRP, head domain
RRM_RdRP1_2	PF26250	1191 - 1259	1.8e-10	RdRP1/2, RRM domain
PH_RDR2	PF24823	1277 - 1428	1.7e-44	RDR2 PH-like domain
RdRP_helical	PF26252	1449 - 1523	5.9e-12	RNA dependent RNA polymerase, helical domain
RdRP	PF05183	1548 - 2119	3.8e-195	RNA dependent RNA polymerase
RdRP_head	PF26253	2140 - 2288	1.1e-46	RdRP, head domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0003605)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14790
fragaria_vesca	FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391 FvH4_4g33391
malus_domestica	MD13G1019300.v1.1 MD13G1041300.v1.1 MD16G1017200.v1.1
prunus_persica	Prupe.1G334600_v2.0.a1 Prupe.1G334600_v2.0.a1
pyrus_communis	pycom13g01640 pycom13g03560 pycom13g03580 pycom16g01590
rosa_chinensis	RchiOBHm_Chr4g0442681 RchiOBHm_Chr4g0442701
rosa_laevigata	RLG00000005990 RLG00000005992
rosa_multiflora	Rmu_ssc0000204.1_g000024 Rmu_ssc0000204.1_g000026
rosa_roxburghii	Rroxscaffold_5G00383490 Rroxscaffold_5G00383510
rosa_rugosa	Rorug04G0342200 Rorug04G0342300
rosa_samantha	Rh4AG394700 Rh4AG394900 Rh4BG407600 Rh4CG423000 Rh4CG423600 Rh4DG401700 Rh4DG401900
rosa_wichuraiana	Rw4G034110 Rw4G034130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	5 cut(s)	426, 756, 1863, 4263, 5553
AarI	CACCTGC	1 cut(s)	5695
AasI	GACNNNNNNGTC	1 cut(s)	2617
Acc36I	ACCTGC	2 cut(s)	4540, 5695
Acc65I	GGTACC	4 cut(s)	3042, 3091, 3492, 6720
AccB1I	GGYRCC	6 cut(s)	154, 410, 3042, 3091, 3492, 6720
AccB7I	CCANNNNNTGG	3 cut(s)	2188, 6022, 6236
AccBSI	CCGCTC	1 cut(s)	6595
AccI	GTMKAC	4 cut(s)	963, 3360, 5390, 6222
AccII	CGCG	3 cut(s)	3540, 3916, 6200
AccIII	TCCGGA	1 cut(s)	1402
AcoI	YGGCCR	2 cut(s)	3644, 3648
AcuI	CTGAAG	7 cut(s)	623, 1038, 1874, 2862, 3906, 3947, 6696
AcvI	CACGTG	2 cut(s)	371, 4010
AcyI	GRCGYC	1 cut(s)	3363
AdeI	CACNNNGTG	2 cut(s)	2188, 6094
AflII	CTTAAG	1 cut(s)	5854
AflIII	ACRYGT	2 cut(s)	864, 1943
AgeI	ACCGGT	2 cut(s)	2534, 3094
AhdI	GACNNNNNGTC	2 cut(s)	2567, 2908
AhlI	ACTAGT	1 cut(s)	2542
AjuI	GAANNNNNNNTTGG	6 cut(s)	1424, 1456, 2399, 2431, 5926, 5958
AleI	CACNNNNGTG	2 cut(s)	3567, 5838
Alw21I	GWGCWC	6 cut(s)	19, 288, 544, 4054, 6090, 6198
Alw26I	GTCTC	5 cut(s)	1275, 1358, 4606, 4690, 6247
Alw44I	GTGCAC	2 cut(s)	284, 540
Ama87I	CYCGRG	1 cut(s)	5990
Aor13HI	TCCGGA	1 cut(s)	1402
ApaLI	GTGCAC	2 cut(s)	284, 540
ArsI	GACNNNNNNTTYG	8 cut(s)	1574, 1606, 3373, 3405, 4393, 4425, 6330, 6362
AseI	ATTAAT	1 cut(s)	2766
AsiGI	ACCGGT	2 cut(s)	2534, 3094
Asp700I	GAANNNNTTC	5 cut(s)	1398, 1770, 2642, 5082, 6679
Asp718I	GGTACC	4 cut(s)	3042, 3091, 3492, 6720
AspLEI	GCGC	4 cut(s)	3345, 3540, 3542, 6858
AsuC2I	CCSGG	2 cut(s)	1996, 3286
AsuII	TTCGAA	1 cut(s)	1617
AvaI	CYCGRG	1 cut(s)	5990
AxyI	CCTNAGG	1 cut(s)	4674
BaeGI	GKGCMC	3 cut(s)	288, 415, 544
BaeI	ACNNNNGTAYC	2 cut(s)	2859, 2892
BamHI	GGATCC	2 cut(s)	766, 4453
BanI	GGYRCC	6 cut(s)	154, 410, 3042, 3091, 3492, 6720
BanII	GRGCYC	4 cut(s)	19, 3557, 4054, 6198
BarI	GAAGNNNNNNTAC	4 cut(s)	1246, 1278, 2835, 2867
BbrPI	CACGTG	2 cut(s)	371, 4010
BbsI	GAAGAC	2 cut(s)	3426, 6305
Bbv12I	GWGCWC	6 cut(s)	19, 288, 544, 4054, 6090, 6198
BceAI	ACGGC	6 cut(s)	55, 1864, 3659, 3897, 4864, 6904
BcgI	CGANNNNNNTGC	8 cut(s)	1191, 1201, 1225, 1235, 6178, 6212, 6316, 6350
BciVI	GTATCC	1 cut(s)	1137
BcnI	CCSGG	2 cut(s)	1996, 3286
BcoDI	GTCTC	5 cut(s)	1275, 1358, 4606, 4690, 6247
BcuI	ACTAGT	1 cut(s)	2542
BfmI	CTRYAG	5 cut(s)	1080, 1107, 4347, 6045, 6219
BfrI	CTTAAG	1 cut(s)	5854
BfuAI	ACCTGC	2 cut(s)	4540, 5695
BfuI	GTATCC	1 cut(s)	1137
BglII	AGATCT	2 cut(s)	1967, 4321
BlpI	GCTNAGC	1 cut(s)	3702
BmcAI	AGTACT	1 cut(s)	1105
BmeRI	GACNNNNNGTC	2 cut(s)	2567, 2908
BmeT110I	CYCGRG	1 cut(s)	5990
BmrI	ACTGGG	1 cut(s)	3079
BmuI	ACTGGG	1 cut(s)	3079
BpiI	GAAGAC	2 cut(s)	3426, 6305
BplI	GAGNNNNNCTC	4 cut(s)	317, 349, 4283, 4315
BpmI	CTGGAG	2 cut(s)	2157, 6705
Bpu10I	CCTNAGC	1 cut(s)	2242
Bpu1102I	GCTNAGC	1 cut(s)	3702
Bpu14I	TTCGAA	1 cut(s)	1617
BpuEI	CTTGAG	3 cut(s)	344, 5513, 6307
BpuMI	CCSGG	2 cut(s)	1996, 3286
Bsa29I	ATCGAT	2 cut(s)	1566, 4722
BsaAI	YACGTR	3 cut(s)	371, 1946, 4010
BsaHI	GRCGYC	1 cut(s)	3363
BsaI	GGTCTC	1 cut(s)	6247
BsaWI	WCCGGW	4 cut(s)	1402, 2534, 3094, 4557
BsaXI	ACNNNNNCTCC	8 cut(s)	87, 117, 430, 460, 1114, 1144, 3363, 3393
Bse118I	RCCGGY	5 cut(s)	2170, 2534, 3094, 3646, 4591
Bse21I	CCTNAGG	1 cut(s)	4674
Bse3DI	GCAATG	7 cut(s)	1758, 2033, 2955, 5667, 6180, 6334, 6363
BseAI	TCCGGA	1 cut(s)	1402
BseCI	ATCGAT	2 cut(s)	1566, 4722
BseMI	GCAATG	7 cut(s)	1758, 2033, 2955, 5667, 6180, 6334, 6363
BsePI	GCGCGC	1 cut(s)	3538
BseRI	GAGGAG	8 cut(s)	9, 108, 230, 1748, 2396, 4044, 4565, 4769
BseSI	GKGCMC	3 cut(s)	288, 415, 544
BseX3I	CGGCCG	1 cut(s)	3644
BseYI	CCCAGC	6 cut(s)	459, 2280, 2332, 2806, 5982, 6034
BsgI	GTGCAG	1 cut(s)	1251
Bsh1236I	CGCG	3 cut(s)	3540, 3916, 6200
Bsh1285I	CGRYCG	2 cut(s)	3258, 3647
BshNI	GGYRCC	6 cut(s)	154, 410, 3042, 3091, 3492, 6720
BshTI	ACCGGT	2 cut(s)	2534, 3094
BshVI	ATCGAT	2 cut(s)	1566, 4722
BsiEI	CGRYCG	2 cut(s)	3258, 3647
BsiHKAI	GWGCWC	6 cut(s)	19, 288, 544, 4054, 6090, 6198
BsiHKCI	CYCGRG	1 cut(s)	5990
BslFI	GGGAC	6 cut(s)	2011, 3919, 4118, 4313, 4771, 5999
BsmAI	GTCTC	5 cut(s)	1275, 1358, 4606, 4690, 6247
BsmFI	GGGAC	6 cut(s)	2011, 3919, 4118, 4313, 4771, 5999
BsmI	GAATGC	2 cut(s)	2243, 4886
Bso31I	GGTCTC	1 cut(s)	6247
BsoBI	CYCGRG	1 cut(s)	5990
Bsp119I	TTCGAA	1 cut(s)	1617
Bsp1286I	GDGCHC	8 cut(s)	19, 288, 415, 544, 3557, 4054, 6090, 6198
Bsp13I	TCCGGA	1 cut(s)	1402
Bsp1407I	TGTACA	4 cut(s)	867, 5440, 5516, 6756
Bsp1720I	GCTNAGC	1 cut(s)	3702
Bsp19I	CCATGG	1 cut(s)	3348
Bsp68I	TCGCGA	1 cut(s)	6200
BspDI	ATCGAT	2 cut(s)	1566, 4722
BspEI	TCCGGA	1 cut(s)	1402
BspFNI	CGCG	3 cut(s)	3540, 3916, 6200
BspHI	TCATGA	1 cut(s)	6058
BspMAI	CTGCAG	2 cut(s)	1111, 4351
BspMI	ACCTGC	2 cut(s)	4540, 5695
BspQI	GCTCTTC	4 cut(s)	1360, 5044, 5650, 6078
BspT104I	TTCGAA	1 cut(s)	1617
BspT107I	GGYRCC	6 cut(s)	154, 410, 3042, 3091, 3492, 6720
BspTI	CTTAAG	1 cut(s)	5854
BspTNI	GGTCTC	1 cut(s)	6247
BsrBI	CCGCTC	1 cut(s)	6595
BsrDI	GCAATG	7 cut(s)	1758, 2033, 2955, 5667, 6180, 6334, 6363
BsrFI	RCCGGY	5 cut(s)	2170, 2534, 3094, 3646, 4591
BsrGI	TGTACA	4 cut(s)	867, 5440, 5516, 6756
BssAI	RCCGGY	5 cut(s)	2170, 2534, 3094, 3646, 4591
BssHII	GCGCGC	1 cut(s)	3538
BssNAI	GTATAC	1 cut(s)	964
BssNI	GRCGYC	1 cut(s)	3363
BssT1I	CCWWGG	8 cut(s)	825, 1708, 2668, 3225, 3348, 5691, 5921, 6588
Bst1107I	GTATAC	1 cut(s)	964
Bst6I	CTCTTC	9 cut(s)	514, 599, 1107, 1360, 3226, 5044, 5388, 5650, 6078
BstACI	GRCGYC	1 cut(s)	3363
BstAFI	CTTAAG	1 cut(s)	5854
BstAPI	GCANNNNNTGC	3 cut(s)	2563, 3814, 5009
BstAUI	TGTACA	4 cut(s)	867, 5440, 5516, 6756
BstBAI	YACGTR	3 cut(s)	371, 1946, 4010
BstBI	TTCGAA	1 cut(s)	1617
BstDSI	CCRYGG	2 cut(s)	3348, 6797
BstEII	GGTNACC	1 cut(s)	2288
BstENI	CCTNNNNNAGG	1 cut(s)	2701
BstFNI	CGCG	3 cut(s)	3540, 3916, 6200
BstHHI	GCGC	4 cut(s)	3345, 3540, 3542, 6858
BstMAI	GTCTC	5 cut(s)	1275, 1358, 4606, 4690, 6247
BstMCI	CGRYCG	2 cut(s)	3258, 3647
BstPI	GGTNACC	1 cut(s)	2288
BstSFI	CTRYAG	5 cut(s)	1080, 1107, 4347, 6045, 6219
BstSLI	GKGCMC	3 cut(s)	288, 415, 544
BstUI	CGCG	3 cut(s)	3540, 3916, 6200
BstV2I	GAAGAC	2 cut(s)	3426, 6305
BstX2I	RGATCY	5 cut(s)	327, 766, 1967, 4321, 4453
BstXI	CCANNNNNNTGG	1 cut(s)	407
BstYI	RGATCY	5 cut(s)	327, 766, 1967, 4321, 4453
BstZ17I	GTATAC	1 cut(s)	964
BstZI	CGGCCG	1 cut(s)	3644
Bsu15I	ATCGAT	2 cut(s)	1566, 4722
Bsu36I	CCTNAGG	1 cut(s)	4674
BsuI	GTATCC	1 cut(s)	1137
BsuTUI	ATCGAT	2 cut(s)	1566, 4722
BtgI	CCRYGG	2 cut(s)	3348, 6797
BtsI	GCAGTG	2 cut(s)	105, 4017
BtuMI	TCGCGA	1 cut(s)	6200
BveI	ACCTGC	2 cut(s)	4540, 5695
CciI	TCATGA	1 cut(s)	6058
CfoI	GCGC	4 cut(s)	3345, 3540, 3542, 6858
Cfr10I	RCCGGY	5 cut(s)	2170, 2534, 3094, 3646, 4591
ClaI	ATCGAT	2 cut(s)	1566, 4722
CseI	GACGC	3 cut(s)	3284, 3371, 6614
CsiI	ACCWGGT	1 cut(s)	6650
CspAI	ACCGGT	2 cut(s)	2534, 3094
DraI	TTTAAA	3 cut(s)	382, 1819, 4381
DraIII	CACNNNGTG	2 cut(s)	2188, 6094
DrdI	GACNNNNNNGTC	1 cut(s)	2617
DriI	GACNNNNNGTC	2 cut(s)	2567, 2908
DseDI	GACNNNNNNGTC	1 cut(s)	2617
EaeI	YGGCCR	2 cut(s)	3644, 3648
EagI	CGGCCG	1 cut(s)	3644
Eam1104I	CTCTTC	9 cut(s)	514, 599, 1107, 1360, 3226, 5044, 5388, 5650, 6078
Eam1105I	GACNNNNNGTC	2 cut(s)	2567, 2908
EarI	CTCTTC	9 cut(s)	514, 599, 1107, 1360, 3226, 5044, 5388, 5650, 6078
EciI	GGCGGA	4 cut(s)	34, 191, 1440, 3315
Ecl136II	GAGCTC	3 cut(s)	17, 4052, 6196
EclXI	CGGCCG	1 cut(s)	3644
Eco130I	CCWWGG	8 cut(s)	825, 1708, 2668, 3225, 3348, 5691, 5921, 6588
Eco147I	AGGCCT	4 cut(s)	921, 2224, 3295, 5926
Eco24I	GRGCYC	4 cut(s)	19, 3557, 4054, 6198
Eco31I	GGTCTC	1 cut(s)	6247
Eco32I	GATATC	6 cut(s)	210, 1024, 1464, 3007, 4372, 4720
Eco52I	CGGCCG	1 cut(s)	3644
Eco53kI	GAGCTC	3 cut(s)	17, 4052, 6196
Eco57I	CTGAAG	7 cut(s)	623, 1038, 1874, 2862, 3906, 3947, 6696
Eco72I	CACGTG	2 cut(s)	371, 4010
Eco81I	CCTNAGG	1 cut(s)	4674
Eco88I	CYCGRG	1 cut(s)	5990
Eco91I	GGTNACC	1 cut(s)	2288
EcoICRI	GAGCTC	3 cut(s)	17, 4052, 6196
EcoNI	CCTNNNNNAGG	1 cut(s)	2701
EcoO109I	RGGNCCY	4 cut(s)	1258, 3126, 5986, 6648
EcoO65I	GGTNACC	1 cut(s)	2288
EcoRI	GAATTC	3 cut(s)	1228, 2576, 4912
EcoRV	GATATC	6 cut(s)	210, 1024, 1464, 3007, 4372, 4720
EcoT14I	CCWWGG	8 cut(s)	825, 1708, 2668, 3225, 3348, 5691, 5921, 6588
EcoT22I	ATGCAT	2 cut(s)	3810, 5766
EcoT38I	GRGCYC	4 cut(s)	19, 3557, 4054, 6198
ErhI	CCWWGG	8 cut(s)	825, 1708, 2668, 3225, 3348, 5691, 5921, 6588
FaqI	GGGAC	6 cut(s)	2011, 3919, 4118, 4313, 4771, 5999
FauI	CCCGC	1 cut(s)	6588
FauNDI	CATATG	2 cut(s)	4366, 5886
FblI	GTMKAC	4 cut(s)	963, 3360, 5390, 6222
FriOI	GRGCYC	4 cut(s)	19, 3557, 4054, 6198
FseI	GGCCGGCC	1 cut(s)	3650
GlaI	GCGC	4 cut(s)	3344, 3539, 3541, 6857
GsaI	CCCAGC	6 cut(s)	463, 2284, 2336, 2810, 5986, 6038
GsuI	CTGGAG	2 cut(s)	2157, 6705
HgaI	GACGC	3 cut(s)	3284, 3371, 6614
HhaI	GCGC	4 cut(s)	3345, 3540, 3542, 6858
Hin1I	GRCGYC	1 cut(s)	3363
Hin6I	GCGC	4 cut(s)	3343, 3538, 3540, 6856
HinP1I	GCGC	4 cut(s)	3343, 3538, 3540, 6856
HincII	GTYRAC	2 cut(s)	1685, 3361
HindII	GTYRAC	2 cut(s)	1685, 3361
HindIII	AAGCTT	4 cut(s)	320, 2639, 3759, 6708
Hpy99I	CGWCG	4 cut(s)	1213, 3278, 3365, 3569
HpyCH4IV	ACGT	5 cut(s)	370, 1945, 3354, 4009, 6775
HpySE526I	ACGT	5 cut(s)	370, 1945, 3354, 4009, 6775
Hsp92I	GRCGYC	1 cut(s)	3363
HspAI	GCGC	4 cut(s)	3343, 3538, 3540, 6856
KflI	GGGWCCC	1 cut(s)	5986
Kpn2I	TCCGGA	1 cut(s)	1402
KpnI	GGTACC	4 cut(s)	3046, 3095, 3496, 6724
KroI	GCCGGC	3 cut(s)	2170, 3646, 4591
KroNI	GCCGGC	3 cut(s)	2172, 3648, 4593
LguI	GCTCTTC	4 cut(s)	1360, 5044, 5650, 6078
MabI	ACCWGGT	1 cut(s)	6650
MaeII	ACGT	5 cut(s)	370, 1945, 3354, 4009, 6775
MbiI	CCGCTC	1 cut(s)	6595
MfeI	CAATTG	3 cut(s)	4901, 6069, 6213
MflI	RGATCY	5 cut(s)	327, 766, 1967, 4321, 4453
MhlI	GDGCHC	8 cut(s)	19, 288, 415, 544, 3557, 4054, 6090, 6198
MmeI	TCCRAC	6 cut(s)	1595, 2671, 5282, 5595, 6040, 6495
Mph1103I	ATGCAT	2 cut(s)	3810, 5766
MroI	TCCGGA	1 cut(s)	1402
MroNI	GCCGGC	3 cut(s)	2170, 3646, 4591
MroXI	GAANNNNTTC	5 cut(s)	1398, 1770, 2642, 5082, 6679
MslI	CAYNNNNRTG	7 cut(s)	535, 2232, 2511, 3037, 3567, 5838, 5934
MspA1I	CMGCKG	8 cut(s)	459, 1762, 2305, 3770, 5153, 5438, 6248, 6381
MspCI	CTTAAG	1 cut(s)	5854
MunI	CAATTG	3 cut(s)	4901, 6069, 6213
Mva1269I	GAATGC	2 cut(s)	2243, 4886
MvnI	CGCG	3 cut(s)	3540, 3916, 6200
NaeI	GCCGGC	3 cut(s)	2172, 3648, 4593
NciI	CCSGG	2 cut(s)	1996, 3286
NcoI	CCATGG	1 cut(s)	3348
NdeI	CATATG	2 cut(s)	4366, 5886
NgoMIV	GCCGGC	3 cut(s)	2170, 3646, 4591
NmeAIII	GCCGAG	2 cut(s)	3576, 6832
NruI	TCGCGA	1 cut(s)	6200
NsiI	ATGCAT	2 cut(s)	3810, 5766
NspV	TTCGAA	1 cut(s)	1617
OliI	CACNNNNGTG	2 cut(s)	3567, 5838
PaeI	GCATGC	1 cut(s)	879
PagI	TCATGA	1 cut(s)	6058
PaqCI	CACCTGC	1 cut(s)	5695
PasI	CCCWGGG	1 cut(s)	3212
PauI	GCGCGC	1 cut(s)	3538
PceI	AGGCCT	4 cut(s)	921, 2224, 3295, 5926
PciI	ACATGT	1 cut(s)	864
PciSI	GCTCTTC	4 cut(s)	1360, 5044, 5650, 6078
PcsI	WCGNNNNNNNCGW	1 cut(s)	1003
PctI	GAATGC	2 cut(s)	2243, 4886
PdiI	GCCGGC	3 cut(s)	2172, 3648, 4593
PdmI	GAANNNNTTC	5 cut(s)	1398, 1770, 2642, 5082, 6679
PflFI	GACNNNGTC	2 cut(s)	3278, 3356
PflMI	CCANNNNNTGG	3 cut(s)	2188, 6022, 6236
PinAI	ACCGGT	2 cut(s)	2534, 3094
PmaCI	CACGTG	2 cut(s)	371, 4010
PmlI	CACGTG	2 cut(s)	371, 4010
Ppu21I	YACGTR	3 cut(s)	371, 1946, 4010
PpuMI	RGGWCCY	2 cut(s)	5986, 6648
PscI	ACATGT	1 cut(s)	864
PshBI	ATTAAT	1 cut(s)	2766
PsiI	TTATAA	5 cut(s)	426, 756, 1863, 4263, 5553
Psp124BI	GAGCTC	3 cut(s)	19, 4054, 6198
Psp5II	RGGWCCY	2 cut(s)	5986, 6648
PspCI	CACGTG	2 cut(s)	371, 4010
PspEI	GGTNACC	1 cut(s)	2288
PspFI	CCCAGC	6 cut(s)	459, 2280, 2332, 2806, 5982, 6034
PspPPI	RGGWCCY	2 cut(s)	5986, 6648
PsrI	GAACNNNNNNTAC	2 cut(s)	4620, 4652
PstI	CTGCAG	2 cut(s)	1111, 4351
PsuI	RGATCY	5 cut(s)	327, 766, 1967, 4321, 4453
PsyI	GACNNNGTC	2 cut(s)	3278, 3356
PteI	GCGCGC	1 cut(s)	3538
PvuII	CAGCTG	5 cut(s)	1762, 5153, 5438, 6248, 6381
RigI	GGCCGGCC	1 cut(s)	3650
RruI	TCGCGA	1 cut(s)	6200
RseI	CAYNNNNRTG	7 cut(s)	535, 2232, 2511, 3037, 3567, 5838, 5934
SacI	GAGCTC	3 cut(s)	19, 4054, 6198
SalI	GTCGAC	1 cut(s)	3359
SapI	GCTCTTC	4 cut(s)	1360, 5044, 5650, 6078
SbfI	CCTGCAGG	1 cut(s)	4351
ScaI	AGTACT	1 cut(s)	1105
SdaI	CCTGCAGG	1 cut(s)	4351
SduI	GDGCHC	8 cut(s)	19, 288, 415, 544, 3557, 4054, 6090, 6198
SexAI	ACCWGGT	1 cut(s)	6650
SfcI	CTRYAG	5 cut(s)	1080, 1107, 4347, 6045, 6219
SfuI	TTCGAA	1 cut(s)	1617
SmiMI	CAYNNNNRTG	7 cut(s)	535, 2232, 2511, 3037, 3567, 5838, 5934
SmlI	CTYRAG	4 cut(s)	323, 5528, 5854, 6322
SmoI	CTYRAG	4 cut(s)	323, 5528, 5854, 6322
SpeI	ACTAGT	1 cut(s)	2542
SphI	GCATGC	1 cut(s)	879
Sse8387I	CCTGCAGG	1 cut(s)	4351
SseBI	AGGCCT	4 cut(s)	921, 2224, 3295, 5926
SspI	AATATT	2 cut(s)	1436, 6549
SstI	GAGCTC	3 cut(s)	19, 4054, 6198
StuI	AGGCCT	4 cut(s)	921, 2224, 3295, 5926
StyI	CCWWGG	8 cut(s)	825, 1708, 2668, 3225, 3348, 5691, 5921, 6588
TaiI	ACGT	5 cut(s)	373, 1948, 3357, 4012, 6778
TaqII	GACCGA	2 cut(s)	3148, 6317
TatI	WGTACW	6 cut(s)	867, 1103, 2539, 5440, 5516, 6756
TauI	GCSGC	2 cut(s)	50, 2557
TspGWI	ACGGA	3 cut(s)	1464, 4645, 6174
Tth111I	GACNNNGTC	2 cut(s)	3278, 3356
Van91I	CCANNNNNTGG	3 cut(s)	2188, 6022, 6236
Vha464I	CTTAAG	1 cut(s)	5854
VneI	GTGCAC	2 cut(s)	284, 540
VspI	ATTAAT	1 cut(s)	2766
XagI	CCTNNNNNAGG	1 cut(s)	2701
XbaI	TCTAGA	3 cut(s)	1984, 5674, 6868
XmiI	GTMKAC	4 cut(s)	963, 3360, 5390, 6222
XmnI	GAANNNNTTC	5 cut(s)	1398, 1770, 2642, 5082, 6679
ZrmI	AGTACT	1 cut(s)	1105
Zsp2I	ATGCAT	2 cut(s)	3810, 5766

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.