Rmu_ssc0000253.1_g000008

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000253.1
OG0003764
Physical Location & Seq
Forward (+)
30983 .. 32252
1270 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 543 bp
atgaagataaacagagctgtttgggggccgttgaacagcactatcataagcgccggagaagatgctgtcattcgtatctgggattctgagactggaaaactacttcaagagaaccagaaggaagagggtcacacgaaaacgatcacttcactagttaaatctccggatggttcacactttctaaccggttcccttgataaatctgcgaagctttgggatactagaacattgactcttatcaagacataccggactgagagtccagtcaatgcagttgcaatgtcgcctcttctcaatcatgttgtacttggtggtggtcaggatgcaatcaatgtcaccacaactgatcatcgtgctgggaaattcgaagccaaattctatgacaagattcttcaagaagagattggaggtgttaaagggcattttggacctattaatgctttggctttcaaccctgatggtaaaagtttctcaagtggaggtgaagatggttacatacgtctacatcacttcgaccatgattacttcaacatcaagatttag

Protein Analysis

180

Amino Acids

19.82

Weight (kDa)

6.44

Isoelectric Point (pI)

36.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 2 - 179 7.8e-69 EIF3I
Beta-prop_THOC3 PF25174 3 - 124 1.9e-18 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 3 - 99 3.4e-14 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 3 - 102 3.3e-06 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 7 - 114 2.1e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 8 - 112 8.7e-11 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 11 - 166 2.7e-06 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 12 - 168 1.3e-15 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 12 - 172 3.8e-11 WDHD1 first WD40 domain
WD40_Gbeta PF25391 14 - 120 9.5e-13 G protein beta WD-40 repeat protein
WDR55 PF24796 15 - 128 1.6e-07 WDR55
Beta-prop_EML PF23409 20 - 166 2.6e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 21 - 98 3.5e-09 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 42 - 73 8.7e-09 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 50 - 177 2.7e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 137 - 168 3.6e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 502
AccIII TCCGGA 1 cut(s) 163
AcsI RAATTY 2 cut(s) 362, 374
AfaI GTAC 1 cut(s) 306
AgeI ACCGGT 1 cut(s) 185
AgsI TTSAA 5 cut(s) 34, 107, 395, 451, 529
AhdI GACNNNNNGTC 1 cut(s) 258
AhlI ACTAGT 1 cut(s) 151
AluBI AGCT 2 cut(s) 17, 211
AluI AGCT 2 cut(s) 17, 211
Alw26I GTCTC 1 cut(s) 83
Aor13HI TCCGGA 1 cut(s) 163
AoxI GGCC 1 cut(s) 26
ApoI RAATTY 2 cut(s) 362, 374
AseI ATTAAT 1 cut(s) 435
AsiGI ACCGGT 1 cut(s) 185
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 2 cut(s) 26, 428
AsuHPI GGTGA 2 cut(s) 328, 494
AsuII TTCGAA 1 cut(s) 366
AvaII GGWCC 1 cut(s) 428
BccI CCATC 3 cut(s) 161, 452, 482
BceAI ACGGC 1 cut(s) 13
BciVI GTATCC 1 cut(s) 211
BclI TGATCA 1 cut(s) 346
BcoDI GTCTC 1 cut(s) 83
BcuI ACTAGT 1 cut(s) 151
BfaI CTAG 2 cut(s) 152, 222
BfoI RGCGCY 1 cut(s) 54
BfuI GTATCC 1 cut(s) 211
Bme18I GGWCC 1 cut(s) 428
BmeRI GACNNNNNGTC 1 cut(s) 258
BmgT120I GGNCC 2 cut(s) 26, 428
BmiI GGNNCC 2 cut(s) 27, 190
BmsI GCATC 2 cut(s) 52, 313
Bpu14I TTCGAA 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 457
BsaWI WCCGGW 3 cut(s) 163, 185, 249
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 97, 263
Bse3DI GCAATG 1 cut(s) 285
BseAI TCCGGA 1 cut(s) 163
BseGI GGATG 2 cut(s) 172, 328
BseMI GCAATG 1 cut(s) 285
BseMII CTCAG 2 cut(s) 78, 246
BseNI ACTGG 2 cut(s) 97, 263
BseYI CCCAGC 1 cut(s) 356
BshFI GGCC 1 cut(s) 28
BshTI ACCGGT 1 cut(s) 185
BsiSI CCGG 4 cut(s) 54, 164, 186, 250
BsmAI GTCTC 1 cut(s) 83
BsnI GGCC 1 cut(s) 28
Bsp119I TTCGAA 1 cut(s) 366
Bsp13I TCCGGA 1 cut(s) 163
Bsp143I GATC 2 cut(s) 141, 346
BspANI GGCC 1 cut(s) 28
BspCNI CTCAG 2 cut(s) 79, 247
BspEI TCCGGA 1 cut(s) 163
BspLI GGNNCC 2 cut(s) 27, 190
BspT104I TTCGAA 1 cut(s) 366
BsrDI GCAATG 1 cut(s) 285
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 2 cut(s) 97, 263
BssAI RCCGGY 1 cut(s) 185
BssMI GATC 2 cut(s) 141, 346
Bst6I CTCTTC 3 cut(s) 117, 294, 393
BstBI TTCGAA 1 cut(s) 366
BstDEI CTNAG 2 cut(s) 87, 255
BstF5I GGATG 2 cut(s) 172, 328
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 2 cut(s) 144, 349
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 2 cut(s) 141, 346
BsuI GTATCC 1 cut(s) 211
BsuRI GGCC 1 cut(s) 28
BtsCI GGATG 2 cut(s) 172, 328
CfoI GCGC 1 cut(s) 53
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 2 cut(s) 26, 428
Csp6I GTAC 1 cut(s) 305
CspAI ACCGGT 1 cut(s) 185
CviAII CATG 2 cut(s) 299, 518
CviJI RGCY 5 cut(s) 17, 28, 211, 371, 446
CviKI_1 RGCY 5 cut(s) 17, 28, 211, 371, 446
CviQI GTAC 1 cut(s) 305
DdeI CTNAG 2 cut(s) 87, 255
DpnI GATC 2 cut(s) 143, 348
DpnII GATC 2 cut(s) 141, 346
DriI GACNNNNNGTC 1 cut(s) 258
Eam1104I CTCTTC 3 cut(s) 117, 294, 393
Eam1105I GACNNNNNGTC 1 cut(s) 258
EarI CTCTTC 3 cut(s) 117, 294, 393
Eco47I GGWCC 1 cut(s) 428
FaeI CATG 2 cut(s) 302, 521
FaiI YATR 6 cut(s) 47, 247, 300, 381, 497, 519
FatI CATG 2 cut(s) 298, 517
FbaI TGATCA 1 cut(s) 346
FblI GTMKAC 1 cut(s) 502
FokI GGATG 2 cut(s) 179, 335
FspBI CTAG 2 cut(s) 152, 222
GlaI GCGC 1 cut(s) 52
GsaI CCCAGC 1 cut(s) 360
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 1 cut(s) 28
HapII CCGG 4 cut(s) 54, 164, 186, 250
HhaI GCGC 1 cut(s) 53
Hin1II CATG 2 cut(s) 302, 521
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HindIII AAGCTT 1 cut(s) 209
HinfI GANTC 4 cut(s) 83, 232, 259, 388
HpaII CCGG 4 cut(s) 54, 164, 186, 250
HphI GGTGA 2 cut(s) 328, 494
Hpy166II GTNNAC 2 cut(s) 173, 503
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 6 cut(s) 107, 164, 241, 320, 395, 535
Hpy8I GTNNAC 2 cut(s) 173, 503
HpyAV CCTTC 1 cut(s) 112
HpyCH4IV ACGT 1 cut(s) 499
HpyCH4V TGCA 3 cut(s) 272, 278, 326
HpyF3I CTNAG 2 cut(s) 87, 255
HpySE526I ACGT 1 cut(s) 499
Hsp92II CATG 2 cut(s) 302, 521
HspAI GCGC 1 cut(s) 51
Kpn2I TCCGGA 1 cut(s) 163
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 2 cut(s) 141, 346
LweI GCATC 2 cut(s) 52, 313
MaeI CTAG 2 cut(s) 152, 222
MaeII ACGT 1 cut(s) 499
MaeIII GTNAC 3 cut(s) 128, 334, 491
MalI GATC 2 cut(s) 143, 348
MboI GATC 2 cut(s) 141, 346
MboII GAAGA 7 cut(s) 16, 71, 134, 281, 383, 410, 497
MluCI AATT 2 cut(s) 362, 374
MlyI GAGTC 2 cut(s) 226, 268
MnlI CCTC 4 cut(s) 118, 297, 401, 473
MroI TCCGGA 1 cut(s) 163
MseI TTAA 3 cut(s) 156, 414, 435
MspI CCGG 4 cut(s) 54, 164, 186, 250
NdeII GATC 2 cut(s) 141, 346
NlaIII CATG 2 cut(s) 302, 521
NlaIV GGNNCC 2 cut(s) 27, 190
NmuCI GTSAC 2 cut(s) 128, 334
NspV TTCGAA 1 cut(s) 366
PfeI GAWTC 2 cut(s) 83, 388
PinAI ACCGGT 1 cut(s) 185
PleI GAGTC 2 cut(s) 226, 267
PpsI GAGTC 2 cut(s) 226, 267
PshBI ATTAAT 1 cut(s) 435
PspFI CCCAGC 1 cut(s) 356
PspN4I GGNNCC 2 cut(s) 27, 190
PspPI GGNCC 2 cut(s) 26, 428
RsaI GTAC 1 cut(s) 306
RsaNI GTAC 1 cut(s) 305
SaqAI TTAA 3 cut(s) 156, 414, 435
Sau3AI GATC 2 cut(s) 141, 346
Sau96I GGNCC 2 cut(s) 26, 428
SchI GAGTC 2 cut(s) 226, 268
SetI ASST 6 cut(s) 19, 213, 412, 433, 484, 502
SfaNI GCATC 2 cut(s) 52, 313
SfuI TTCGAA 1 cut(s) 366
SinI GGWCC 1 cut(s) 428
SmlI CTYRAG 1 cut(s) 472
SmoI CTYRAG 1 cut(s) 472
SpeI ACTAGT 1 cut(s) 151
Sse9I AATT 2 cut(s) 362, 374
SspMI CTAG 2 cut(s) 152, 222
TaiI ACGT 1 cut(s) 502
TaqI TCGA 2 cut(s) 366, 513
TasI AATT 2 cut(s) 362, 374
TatI WGTACW 1 cut(s) 304
TfiI GAWTC 2 cut(s) 83, 388
Tru1I TTAA 3 cut(s) 156, 414, 435
Tru9I TTAA 3 cut(s) 156, 414, 435
TseFI GTSAC 2 cut(s) 128, 334
Tsp45I GTSAC 2 cut(s) 128, 334
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 428
VspI ATTAAT 1 cut(s) 435
XapI RAATTY 2 cut(s) 362, 374
XmiI GTMKAC 1 cut(s) 502
XspI CTAG 2 cut(s) 152, 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.