Rmu_ssc0000297.1_g000005

Maf-like protein DDB_G0281937 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000297.1
OG0002073
Physical Location & Seq
Forward (+)
27793 .. 30018
2226 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 663 bp
atggaaacttctcaggcagaagccatccggcctaagctcccagttgatgactacgtaaaggatgctgaaccaacgctattaattacctctgatcaagtggtagtctatgaaggtgctgtcagggaaaaaccatccagcaaggaagaagcacggcaatttttgaaagactattctggagaacatgcagcaacagtgggatctgtgcttgttacaaaccttaaaacaggattcagtaaaggagaatgggaccgagtggagtccgtcgactttcaatttcatcaaaaacacccctgcggagaacactatcctgcggtcaacgtcgacggagacgagggtctggaggtcgtccgctgcttcggcggcggaggcgaagaagtggcagaggtaggagtgggaggagtggtagaggctgttgaggttgaaggtggcttagaagttttcagggtcagaagaagagtagaaggcaggcttggcagtggggacggtggtggtggtgatgttgaagtttcgtctgcggactaccacttagaggttcacctcgactcccccggaattgacattgtcttggcctgcgactccgctgcgatggctttcatggcggaagaggaagaagatgaggagaggaggagagaggtgggtggagatgacaaagtgtttttttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Maf

Protein Analysis

220

Amino Acids

23.85

Weight (kDa)

4.45

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Maf PF02545 13 - 94 6.6e-10 Maf-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 264, 321
AciI CCGC 8 cut(s) 294, 311, 349, 360, 363, 515, 579, 599
AclWI GGATC 1 cut(s) 205
AfiI CCNNNNNNNGG 1 cut(s) 529
AgsI TTSAA 4 cut(s) 163, 272, 422, 503
AjuI GAANNNNNNNTTGG 2 cut(s) 453, 485
AluBI AGCT 1 cut(s) 37
AluI AGCT 1 cut(s) 37
Alw26I GTCTC 1 cut(s) 321
AlwI GGATC 1 cut(s) 205
AoxI GGCC 2 cut(s) 29, 567
ApeKI GCWGC 3 cut(s) 185, 351, 581
AseI ATTAAT 1 cut(s) 80
AspS9I GGNCC 1 cut(s) 247
AsuC2I CCSGG 1 cut(s) 549
AsuHPI GGTGA 2 cut(s) 506, 527
AvaII GGWCC 1 cut(s) 247
BbvI GCAGC 3 cut(s) 197, 338, 568
BccI CCATC 3 cut(s) 32, 139, 580
BceAI ACGGC 1 cut(s) 167
BcgI CGANNNNNNTGC 2 cut(s) 563, 597
BclI TGATCA 1 cut(s) 91
BcnI CCSGG 1 cut(s) 549
BcoDI GTCTC 1 cut(s) 321
BisI GCNGC 4 cut(s) 186, 352, 361, 582
BlsI GCNGC 4 cut(s) 187, 353, 362, 583
Bme1390I CCNGG 1 cut(s) 549
Bme18I GGWCC 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 1 cut(s) 248
BmrFI CCNGG 1 cut(s) 549
BmrI ACTGGG 1 cut(s) 35
BmsI GCATC 1 cut(s) 52
BmuI ACTGGG 1 cut(s) 35
BoxI GACNNNNGTC 1 cut(s) 333
BpmI CTGGAG 2 cut(s) 195, 359
Bpu10I CCTNAGC 1 cut(s) 33
BpuMI CCSGG 1 cut(s) 549
BsaAI YACGTR 1 cut(s) 55
BsaJI CCNNGG 1 cut(s) 547
BsaXI ACNNNNNCTCC 4 cut(s) 387, 417, 527, 557
Bsc4I CCNNNNNNNGG 1 cut(s) 529
Bse1I ACTGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 547
BseGI GGATG 3 cut(s) 24, 67, 131
BseLI CCNNNNNNNGG 1 cut(s) 529
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 1 cut(s) 41
BseRI GAGGAG 4 cut(s) 411, 632, 637, 640
BseXI GCAGC 3 cut(s) 197, 338, 568
BshFI GGCC 2 cut(s) 31, 569
BsiSI CCGG 2 cut(s) 28, 549
BslFI GGGAC 2 cut(s) 260, 494
BslI CCNNNNNNNGG 1 cut(s) 529
BsmAI GTCTC 1 cut(s) 321
BsmBI CGTCTC 1 cut(s) 321
BsmFI GGGAC 2 cut(s) 260, 494
BsnI GGCC 2 cut(s) 31, 569
Bsp143I GATC 2 cut(s) 91, 197
BspACI CCGC 8 cut(s) 294, 311, 349, 360, 363, 515, 579, 599
BspANI GGCC 2 cut(s) 31, 569
BspCNI CTCAG 1 cut(s) 25
BspLI GGNNCC 1 cut(s) 248
BspPI GGATC 1 cut(s) 205
BsrI ACTGG 1 cut(s) 41
BssECI CCNNGG 1 cut(s) 547
BssMI GATC 2 cut(s) 91, 197
Bst4CI ACNGT 2 cut(s) 193, 485
Bst6I CTCTTC 2 cut(s) 448, 597
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 2 cut(s) 467, 571
BstDEI CTNAG 4 cut(s) 12, 33, 430, 526
BstF5I GGATG 3 cut(s) 24, 67, 131
BstKTI GATC 2 cut(s) 94, 200
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 2 cut(s) 91, 197
BstMWI GCNNNNNNNGC 6 cut(s) 357, 360, 366, 471, 587, 596
BstNSI RCATGY 1 cut(s) 185
BstPAI GACNNNNGTC 1 cut(s) 333
BstSCI CCNGG 1 cut(s) 547
BstSNI TACGTA 1 cut(s) 55
BstV1I GCAGC 3 cut(s) 197, 338, 568
BstX2I RGATCY 1 cut(s) 197
BstYI RGATCY 1 cut(s) 197
BsuRI GGCC 2 cut(s) 31, 569
BtgZI GCGATG 1 cut(s) 599
BtsCI GGATG 3 cut(s) 24, 67, 131
BtsI GCAGTG 1 cut(s) 481
BtsIMutI CAGTG 2 cut(s) 198, 481
Cac8I GCNNGC 2 cut(s) 467, 571
Cfr13I GGNCC 1 cut(s) 247
CviAII CATG 2 cut(s) 182, 595
CviJI RGCY 8 cut(s) 23, 31, 37, 410, 429, 469, 569, 590
CviKI_1 RGCY 8 cut(s) 23, 31, 37, 410, 429, 469, 569, 590
DdeI CTNAG 4 cut(s) 12, 33, 430, 526
DpnI GATC 2 cut(s) 93, 199
DpnII GATC 2 cut(s) 91, 197
Eam1104I CTCTTC 2 cut(s) 448, 597
EarI CTCTTC 2 cut(s) 448, 597
EciI GGCGGA 2 cut(s) 378, 614
Eco105I TACGTA 1 cut(s) 55
Eco47I GGWCC 1 cut(s) 247
Esp3I CGTCTC 1 cut(s) 321
FaeI CATG 2 cut(s) 185, 598
FaiI YATR 3 cut(s) 108, 183, 596
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FaqI GGGAC 2 cut(s) 260, 494
FatI CATG 2 cut(s) 181, 594
FbaI TGATCA 1 cut(s) 91
FblI GTMKAC 2 cut(s) 264, 321
Fnu4HI GCNGC 4 cut(s) 186, 352, 361, 582
FokI GGATG 3 cut(s) 11, 74, 118
Fsp4HI GCNGC 4 cut(s) 186, 352, 361, 582
GluI GCNGC 4 cut(s) 186, 352, 361, 582
GsuI CTGGAG 2 cut(s) 195, 359
HaeIII GGCC 2 cut(s) 31, 569
HapII CCGG 2 cut(s) 28, 549
Hin1II CATG 2 cut(s) 185, 598
HincII GTYRAC 3 cut(s) 265, 316, 322
HindII GTYRAC 3 cut(s) 265, 316, 322
HinfI GANTC 4 cut(s) 228, 257, 542, 575
HpaII CCGG 2 cut(s) 28, 549
HphI GGTGA 2 cut(s) 506, 527
Hpy166II GTNNAC 4 cut(s) 265, 316, 322, 535
Hpy188I TCNGA 2 cut(s) 91, 449
Hpy188III TCNNGA 2 cut(s) 174, 338
Hpy8I GTNNAC 4 cut(s) 265, 316, 322, 535
Hpy99I CGWCG 3 cut(s) 266, 323, 326
HpyAV CCTTC 3 cut(s) 104, 416, 455
HpyCH4III ACNGT 2 cut(s) 193, 485
HpyCH4IV ACGT 2 cut(s) 54, 318
HpyCH4V TGCA 1 cut(s) 185
HpyF10VI GCNNNNNNNGC 6 cut(s) 357, 360, 366, 471, 587, 596
HpyF3I CTNAG 4 cut(s) 12, 33, 430, 526
HpySE526I ACGT 2 cut(s) 54, 318
Hsp92II CATG 2 cut(s) 185, 598
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 2 cut(s) 91, 197
LmnI GCTCC 1 cut(s) 42
Lsp1109I GCAGC 3 cut(s) 197, 338, 568
LweI GCATC 1 cut(s) 52
MaeII ACGT 2 cut(s) 54, 318
MaeIII GTNAC 1 cut(s) 208
MalI GATC 2 cut(s) 93, 199
MboI GATC 2 cut(s) 91, 197
MboII GAAGA 7 cut(s) 155, 383, 462, 465, 614, 620, 623
MflI RGATCY 1 cut(s) 197
MluCI AATT 4 cut(s) 81, 155, 272, 552
MlyI GAGTC 3 cut(s) 266, 536, 569
MseI TTAA 3 cut(s) 80, 219, 661
MspA1I CMGCKG 2 cut(s) 351, 581
MspI CCGG 2 cut(s) 28, 549
MspR9I CCNGG 1 cut(s) 549
MwoI GCNNNNNNNGC 6 cut(s) 357, 360, 366, 471, 587, 596
NciI CCSGG 1 cut(s) 549
NdeII GATC 2 cut(s) 91, 197
NlaIII CATG 2 cut(s) 185, 598
NlaIV GGNNCC 1 cut(s) 248
NspI RCATGY 1 cut(s) 185
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PfeI GAWTC 1 cut(s) 228
PflFI GACNNNGTC 1 cut(s) 560
PkrI GCNGC 4 cut(s) 187, 353, 362, 583
PleI GAGTC 3 cut(s) 265, 536, 569
PpsI GAGTC 3 cut(s) 265, 536, 569
Ppu21I YACGTR 1 cut(s) 55
PshAI GACNNNNGTC 1 cut(s) 333
PshBI ATTAAT 1 cut(s) 80
PspN4I GGNNCC 1 cut(s) 248
PspPI GGNCC 1 cut(s) 247
PsuI RGATCY 1 cut(s) 197
PsyI GACNNNGTC 1 cut(s) 560
SalI GTCGAC 2 cut(s) 263, 320
SaqAI TTAA 3 cut(s) 80, 219, 661
SatI GCNGC 4 cut(s) 186, 352, 361, 582
Sau3AI GATC 2 cut(s) 91, 197
Sau96I GGNCC 1 cut(s) 247
SchI GAGTC 3 cut(s) 266, 536, 569
ScrFI CCNGG 1 cut(s) 549
SfaNI GCATC 1 cut(s) 52
SgrDI CGTCGACG 1 cut(s) 320
SinI GGWCC 1 cut(s) 247
SnaBI TACGTA 1 cut(s) 55
Sse9I AATT 4 cut(s) 81, 155, 272, 552
SsiI CCGC 8 cut(s) 294, 311, 349, 360, 363, 515, 579, 599
StyD4I CCNGG 1 cut(s) 547
TaaI ACNGT 2 cut(s) 193, 485
TaiI ACGT 2 cut(s) 57, 321
TaqI TCGA 3 cut(s) 264, 321, 540
TaqII GACCGA 1 cut(s) 264
TasI AATT 4 cut(s) 81, 155, 272, 552
TauI GCSGC 1 cut(s) 363
TfiI GAWTC 1 cut(s) 228
Tru1I TTAA 3 cut(s) 80, 219, 661
Tru9I TTAA 3 cut(s) 80, 219, 661
TscAI CASTG 2 cut(s) 198, 481
TseI GCWGC 3 cut(s) 185, 351, 581
TspDTI ATGAA 3 cut(s) 123, 266, 583
TspGWI ACGGA 2 cut(s) 250, 339
TspRI CASTG 2 cut(s) 198, 481
Tth111I GACNNNGTC 1 cut(s) 560
VpaK11BI GGWCC 1 cut(s) 247
VspI ATTAAT 1 cut(s) 80
XceI RCATGY 1 cut(s) 185
XmiI GTMKAC 2 cut(s) 264, 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.