Rroxscaffold_1G00018130

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005941
Physical Location & Seq
Reverse (-)
22297879 .. 22298517
639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00018130.1

Sequence Viewer

Length: 456 bp
ATGCATACACATCTTCGCGATCCTCCAAAACTAGCTTGGGACATGAGTAAAATTTCAAGCCGCATCAATTTGTTTCTTCAAACCCAGAATTATAAATCAAATCTGGATGCCCCTAGTACGTGGGGTGGTGACGAGCTTGAGGGTGTAGACGATGTAAGTGACGGAGATGAAGAGGATGAACCGGACGAGGAGTTTCATGGTGGCTCAAGGAGCTTGAGAGCCGAACCGGGTAGAGGGTTCACCGTGGAGCTCCGCATCGGATTCGATCCCAAACCACATGAGCGGGTTGTGTGGGCGAACGTCATCCTCAATTTCTTGCTGGGCGCGGCGGCGCTGTTTGAATTTCTCGGCCTTGGCATTGTTCCACCGCCCGAATCGGACCTCCGTGGGAGGGTAGTTCGGGAGCGAGTTGTGGGCCGGGTTATGGTTCACCGGTGGCGCAAAAATGGGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

151

Amino Acids

17.06

Weight (kDa)

5.49

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 93
AccBSI CCGCTC 1 cut(s) 283
AccI GTMKAC 1 cut(s) 147
AccII CGCG 2 cut(s) 18, 326
AciI CCGC 6 cut(s) 61, 253, 283, 326, 329, 368
AclWI GGATC 2 cut(s) 14, 260
AcsI RAATTY 2 cut(s) 51, 341
AfaI GTAC 1 cut(s) 118
AfiI CCNNNNNNNGG 3 cut(s) 233, 391, 424
AgeI ACCGGT 1 cut(s) 432
AgsI TTSAA 3 cut(s) 57, 80, 341
AluBI AGCT 4 cut(s) 35, 136, 213, 250
AluI AGCT 4 cut(s) 35, 136, 213, 250
Alw21I GWGCWC 1 cut(s) 252
AlwI GGATC 2 cut(s) 14, 260
AoxI GGCC 2 cut(s) 349, 415
ApoI RAATTY 2 cut(s) 51, 341
AsiGI ACCGGT 1 cut(s) 432
AspLEI GCGC 3 cut(s) 326, 334, 441
AspS9I GGNCC 2 cut(s) 379, 415
AsuC2I CCSGG 2 cut(s) 228, 419
AsuHPI GGTGA 3 cut(s) 140, 232, 422
AvaII GGWCC 1 cut(s) 379
BaeGI GKGCMC 1 cut(s) 453
BanII GRGCYC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 252
BcgI CGANNNNNNTGC 2 cut(s) 244, 278
BcnI CCSGG 2 cut(s) 228, 419
BfaI CTAG 2 cut(s) 32, 114
BfoI RGCGCY 1 cut(s) 335
BisI GCNGC 3 cut(s) 61, 327, 330
BlsI GCNGC 3 cut(s) 62, 328, 331
Bme1390I CCNGG 2 cut(s) 228, 419
Bme18I GGWCC 1 cut(s) 379
BmgT120I GGNCC 2 cut(s) 379, 415
BmrFI CCNGG 2 cut(s) 228, 419
BmsI GCATC 3 cut(s) 72, 97, 264
BpuEI CTTGAG 3 cut(s) 158, 190, 235
BpuMI CCSGG 2 cut(s) 228, 419
BsaAI YACGTR 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 243, 352, 385
BsaWI WCCGGW 2 cut(s) 181, 432
Bsc4I CCNNNNNNNGG 3 cut(s) 233, 391, 424
Bse118I RCCGGY 1 cut(s) 432
BseDI CCNNGG 3 cut(s) 243, 352, 385
BseGI GGATG 3 cut(s) 112, 181, 303
BseLI CCNNNNNNNGG 3 cut(s) 233, 391, 424
BseRI GAGGAG 1 cut(s) 203
BseSI GKGCMC 1 cut(s) 453
BseYI CCCAGC 1 cut(s) 319
Bsh1236I CGCG 2 cut(s) 18, 326
BshFI GGCC 2 cut(s) 351, 417
BshTI ACCGGT 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 252
BsiSI CCGG 4 cut(s) 182, 227, 418, 433
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 3 cut(s) 233, 391, 424
BsmFI GGGAC 1 cut(s) 53
BsnI GGCC 2 cut(s) 351, 417
Bsp1286I GDGCHC 2 cut(s) 252, 453
Bsp143I GATC 2 cut(s) 19, 265
Bsp68I TCGCGA 1 cut(s) 18
BspACI CCGC 6 cut(s) 61, 253, 283, 326, 329, 368
BspANI GGCC 2 cut(s) 351, 417
BspFNI CGCG 2 cut(s) 18, 326
BspPI GGATC 2 cut(s) 14, 260
BsrBI CCGCTC 1 cut(s) 283
BsrFI RCCGGY 1 cut(s) 432
BssAI RCCGGY 1 cut(s) 432
BssECI CCNNGG 3 cut(s) 243, 352, 385
BssMI GATC 2 cut(s) 19, 265
BssT1I CCWWGG 1 cut(s) 352
Bst4CI ACNGT 1 cut(s) 244
Bst6I CTCTTC 1 cut(s) 165
BstBAI YACGTR 1 cut(s) 120
BstDSI CCRYGG 2 cut(s) 243, 385
BstF5I GGATG 3 cut(s) 112, 181, 303
BstFNI CGCG 2 cut(s) 18, 326
BstH2I RGCGCY 1 cut(s) 335
BstHHI GCGC 3 cut(s) 326, 334, 441
BstKTI GATC 2 cut(s) 22, 268
BstMBI GATC 2 cut(s) 19, 265
BstMWI GCNNNNNNNGC 1 cut(s) 210
BstSCI CCNGG 2 cut(s) 226, 417
BstSLI GKGCMC 1 cut(s) 453
BstUI CGCG 2 cut(s) 18, 326
BsuRI GGCC 2 cut(s) 351, 417
BtgI CCRYGG 2 cut(s) 243, 385
BtsCI GGATG 3 cut(s) 112, 181, 303
BtuMI TCGCGA 1 cut(s) 18
CfoI GCGC 3 cut(s) 326, 334, 441
Cfr10I RCCGGY 1 cut(s) 432
Cfr13I GGNCC 2 cut(s) 379, 415
Csp6I GTAC 1 cut(s) 117
CspAI ACCGGT 1 cut(s) 432
CviAII CATG 3 cut(s) 43, 197, 278
CviJI RGCY 9 cut(s) 35, 60, 136, 204, 213, 221, 250, 351, 417
CviKI_1 RGCY 9 cut(s) 35, 60, 136, 204, 213, 221, 250, 351, 417
CviQI GTAC 1 cut(s) 117
DpnI GATC 2 cut(s) 21, 267
DpnII GATC 2 cut(s) 19, 265
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Ecl136II GAGCTC 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 352
Eco24I GRGCYC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 379
Eco53kI GAGCTC 1 cut(s) 250
EcoICRI GAGCTC 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 352
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 352
FaeI CATG 3 cut(s) 46, 200, 281
FaiI YATR 6 cut(s) 6, 44, 93, 198, 279, 425
FaqI GGGAC 1 cut(s) 53
FatI CATG 3 cut(s) 42, 196, 277
FauI CCCGC 1 cut(s) 276
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 3 cut(s) 61, 327, 330
FokI GGATG 3 cut(s) 119, 188, 290
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 3 cut(s) 61, 327, 330
FspBI CTAG 2 cut(s) 32, 114
GlaI GCGC 3 cut(s) 325, 333, 440
GluI GCNGC 3 cut(s) 61, 327, 330
GsaI CCCAGC 1 cut(s) 323
HaeII RGCGCY 1 cut(s) 335
HaeIII GGCC 2 cut(s) 351, 417
HapII CCGG 4 cut(s) 182, 227, 418, 433
HhaI GCGC 3 cut(s) 326, 334, 441
Hin1II CATG 3 cut(s) 46, 200, 281
Hin6I GCGC 3 cut(s) 324, 332, 439
HinP1I GCGC 3 cut(s) 324, 332, 439
HinfI GANTC 2 cut(s) 261, 374
HpaII CCGG 4 cut(s) 182, 227, 418, 433
HphI GGTGA 3 cut(s) 140, 232, 422
Hpy166II GTNNAC 3 cut(s) 148, 240, 430
Hpy188I TCNGA 2 cut(s) 260, 379
Hpy188III TCNNGA 3 cut(s) 17, 104, 401
Hpy8I GTNNAC 3 cut(s) 148, 240, 430
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4IV ACGT 2 cut(s) 119, 300
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 210
HpySE526I ACGT 2 cut(s) 119, 300
Hsp92II CATG 3 cut(s) 46, 200, 281
HspAI GCGC 3 cut(s) 324, 332, 439
Kzo9I GATC 2 cut(s) 19, 265
LmnI GCTCC 4 cut(s) 210, 247, 255, 403
LpnPI CCDG 7 cut(s) 89, 98, 195, 240, 305, 431, 446
LweI GCATC 3 cut(s) 72, 97, 264
MaeI CTAG 2 cut(s) 32, 114
MaeII ACGT 2 cut(s) 119, 300
MaeIII GTNAC 2 cut(s) 128, 158
MalI GATC 2 cut(s) 21, 267
MbiI CCGCTC 1 cut(s) 283
MboI GATC 2 cut(s) 19, 265
MboII GAAGA 3 cut(s) 5, 68, 182
MhlI GDGCHC 2 cut(s) 252, 453
MluCI AATT 5 cut(s) 51, 67, 88, 310, 341
MnlI CCTC 8 cut(s) 33, 133, 166, 181, 227, 317, 384, 392
Mph1103I ATGCAT 1 cut(s) 6
MspI CCGG 4 cut(s) 182, 227, 418, 433
MspR9I CCNGG 2 cut(s) 228, 419
MvnI CGCG 2 cut(s) 18, 326
MwoI GCNNNNNNNGC 1 cut(s) 210
NciI CCSGG 2 cut(s) 228, 419
NdeII GATC 2 cut(s) 19, 265
NlaIII CATG 3 cut(s) 46, 200, 281
NmeAIII GCCGAG 1 cut(s) 327
NmuCI GTSAC 2 cut(s) 128, 158
NruI TCGCGA 1 cut(s) 18
NsiI ATGCAT 1 cut(s) 6
PfeI GAWTC 2 cut(s) 261, 374
PinAI ACCGGT 1 cut(s) 432
PkrI GCNGC 3 cut(s) 62, 328, 331
Ppu21I YACGTR 1 cut(s) 120
PsiI TTATAA 1 cut(s) 93
Psp124BI GAGCTC 1 cut(s) 252
PspFI CCCAGC 1 cut(s) 319
PspPI GGNCC 2 cut(s) 379, 415
RruI TCGCGA 1 cut(s) 18
RsaI GTAC 1 cut(s) 118
RsaNI GTAC 1 cut(s) 117
SacI GAGCTC 1 cut(s) 252
SatI GCNGC 3 cut(s) 61, 327, 330
Sau3AI GATC 2 cut(s) 19, 265
Sau96I GGNCC 2 cut(s) 379, 415
ScrFI CCNGG 2 cut(s) 228, 419
SduI GDGCHC 2 cut(s) 252, 453
SetI ASST 7 cut(s) 37, 122, 138, 215, 252, 303, 384
SfaNI GCATC 3 cut(s) 72, 97, 264
SgrAI CRCCGGYG 1 cut(s) 432
SinI GGWCC 1 cut(s) 379
SmlI CTYRAG 3 cut(s) 137, 205, 214
SmoI CTYRAG 3 cut(s) 137, 205, 214
Sse9I AATT 5 cut(s) 51, 67, 88, 310, 341
SsiI CCGC 6 cut(s) 61, 253, 283, 326, 329, 368
SspMI CTAG 2 cut(s) 32, 114
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 226, 417
StyI CCWWGG 1 cut(s) 352
TaaI ACNGT 1 cut(s) 244
TaiI ACGT 2 cut(s) 122, 303
TaqI TCGA 1 cut(s) 264
TasI AATT 5 cut(s) 51, 67, 88, 310, 341
TauI GCSGC 3 cut(s) 63, 329, 332
TfiI GAWTC 2 cut(s) 261, 374
TseFI GTSAC 2 cut(s) 128, 158
Tsp45I GTSAC 2 cut(s) 128, 158
TspDTI ATGAA 3 cut(s) 183, 185, 192
TspGWI ACGGA 2 cut(s) 177, 374
VpaK11BI GGWCC 1 cut(s) 379
XapI RAATTY 2 cut(s) 51, 341
XcmI CCANNNNNNNNNTGG 1 cut(s) 33
XmiI GTMKAC 1 cut(s) 147
XspI CTAG 2 cut(s) 32, 114
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.