Rh5CG259500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0005941
Physical Location & Seq
Reverse (-)
27551219 .. 27551638
420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG259500.1

Sequence Viewer

Length: 420 bp
ATGTTGGCTTTCCGGTTGATTCCTGGCTTTTCTCTAGAGATACTCGAGAGCTTCGTGGGTCTGATGACTCAGAGAAACACTGGGTGCGAGCTAGGGTCAGGGTTCTTGGAGTACTTGTATTTTTTGCTTGGAATTAAAGGGCTTGAGGGAGACGAAGGCGTGCCTATGGATTTGAATGTTGCTTCGCAATCGAAGGAGGAGTCGTCCGCAGCGACGGTGGCTATTCTGGTGGCGGAGTCGAAGCGGCACTCACGGCGGACGTCATCCTCGATTTCTTGCTGGGCGCGGCGGCTTCTCGGAGTTGGCGTTGTTCCACCAGGGGAATCAGACCTCCGTGAGCGGGCTGTTCTGGAGTTGGTTGTGGGCCGGGTTTGGGTTCACCGGTTGCGCGAAAATGGGGCACGAAGTCGAGAGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

139

Amino Acids

15.35

Weight (kDa)

7.83

Isoelectric Point (pI)

50.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 263
AccBSI CCGCTC 1 cut(s) 340
AccII CGCG 2 cut(s) 286, 390
AciI CCGC 7 cut(s) 207, 233, 244, 256, 286, 289, 340
AcyI GRCGYC 1 cut(s) 260
AdeI CACNNNGTG 1 cut(s) 84
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 2 cut(s) 340, 373
AgeI ACCGGT 1 cut(s) 381
AgsI TTSAA 1 cut(s) 175
AjnI CCWGG 2 cut(s) 22, 316
AluBI AGCT 2 cut(s) 51, 91
AluI AGCT 2 cut(s) 51, 91
Alw26I GTCTC 1 cut(s) 144
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 1 cut(s) 364
ApeKI GCWGC 1 cut(s) 209
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AsiGI ACCGGT 1 cut(s) 381
AspLEI GCGC 2 cut(s) 286, 390
AspS9I GGNCC 1 cut(s) 364
AsuC2I CCSGG 1 cut(s) 368
AsuHPI GGTGA 1 cut(s) 371
AvaI CYCGRG 1 cut(s) 44
BaeGI GKGCMC 1 cut(s) 403
BbvI GCAGC 1 cut(s) 221
BceAI ACGGC 1 cut(s) 269
BciT130I CCWGG 2 cut(s) 24, 318
BcnI CCSGG 1 cut(s) 368
BcoDI GTCTC 1 cut(s) 144
BfaI CTAG 2 cut(s) 35, 92
BisI GCNGC 4 cut(s) 210, 245, 287, 290
BlsI GCNGC 4 cut(s) 211, 246, 288, 291
BmcAI AGTACT 1 cut(s) 113
Bme1390I CCNGG 3 cut(s) 24, 318, 368
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 364
BmrFI CCNGG 3 cut(s) 24, 318, 368
BmrI ACTGGG 1 cut(s) 90
BmuI ACTGGG 1 cut(s) 90
BpmI CTGGAG 1 cut(s) 371
BpuEI CTTGAG 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 368
BsaHI GRCGYC 1 cut(s) 260
BsaJI CCNNGG 1 cut(s) 317
BsaWI WCCGGW 2 cut(s) 12, 381
BsaXI ACNNNNNCTCC 6 cut(s) 101, 131, 188, 218, 291, 321
Bsc4I CCNNNNNNNGG 2 cut(s) 340, 373
Bse118I RCCGGY 1 cut(s) 381
Bse1I ACTGG 1 cut(s) 85
BseBI CCWGG 2 cut(s) 24, 318
BseDI CCNNGG 1 cut(s) 317
BseGI GGATG 1 cut(s) 263
BseLI CCNNNNNNNGG 2 cut(s) 340, 373
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 1 cut(s) 85
BseRI GAGGAG 1 cut(s) 212
BseSI GKGCMC 1 cut(s) 403
BseXI GCAGC 1 cut(s) 221
BseYI CCCAGC 1 cut(s) 279
Bsh1236I CGCG 2 cut(s) 286, 390
BshFI GGCC 1 cut(s) 366
BshTI ACCGGT 1 cut(s) 381
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 3 cut(s) 13, 367, 382
BslI CCNNNNNNNGG 2 cut(s) 340, 373
BsmAI GTCTC 1 cut(s) 144
BsmBI CGTCTC 1 cut(s) 144
BsnI GGCC 1 cut(s) 366
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 403
BspACI CCGC 7 cut(s) 207, 233, 244, 256, 286, 289, 340
BspANI GGCC 1 cut(s) 366
BspCNI CTCAG 1 cut(s) 82
BspFNI CGCG 2 cut(s) 286, 390
BsrBI CCGCTC 1 cut(s) 340
BsrFI RCCGGY 1 cut(s) 381
BsrI ACTGG 1 cut(s) 85
BssAI RCCGGY 1 cut(s) 381
BssECI CCNNGG 1 cut(s) 317
BssNI GRCGYC 1 cut(s) 260
Bst2UI CCWGG 2 cut(s) 24, 318
Bst4CI ACNGT 1 cut(s) 217
BstACI GRCGYC 1 cut(s) 260
BstC8I GCNNGC 3 cut(s) 89, 161, 342
BstDEI CTNAG 1 cut(s) 69
BstF5I GGATG 1 cut(s) 263
BstFNI CGCG 2 cut(s) 286, 390
BstHHI GCGC 2 cut(s) 286, 390
BstMAI GTCTC 1 cut(s) 144
BstMWI GCNNNNNNNGC 2 cut(s) 218, 253
BstNI CCWGG 2 cut(s) 24, 318
BstSCI CCNGG 3 cut(s) 22, 316, 366
BstSLI GKGCMC 1 cut(s) 403
BstUI CGCG 2 cut(s) 286, 390
BstV1I GCAGC 1 cut(s) 221
BsuRI GGCC 1 cut(s) 366
BtsCI GGATG 1 cut(s) 263
BtsIMutI CAGTG 1 cut(s) 78
Cac8I GCNNGC 3 cut(s) 89, 161, 342
CfoI GCGC 2 cut(s) 286, 390
Cfr10I RCCGGY 1 cut(s) 381
Cfr13I GGNCC 1 cut(s) 364
Csp6I GTAC 1 cut(s) 112
CspAI ACCGGT 1 cut(s) 381
CviJI RGCY 9 cut(s) 8, 27, 51, 91, 142, 221, 292, 344, 366
CviKI_1 RGCY 9 cut(s) 8, 27, 51, 91, 142, 221, 292, 344, 366
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 1 cut(s) 69
DraIII CACNNNGTG 1 cut(s) 84
EciI GGCGGA 2 cut(s) 248, 271
Eco88I CYCGRG 1 cut(s) 44
EcoRII CCWGG 2 cut(s) 22, 316
Esp3I CGTCTC 1 cut(s) 144
FaiI YATR 1 cut(s) 167
FauI CCCGC 1 cut(s) 333
Fnu4HI GCNGC 4 cut(s) 210, 245, 287, 290
FokI GGATG 1 cut(s) 250
Fsp4HI GCNGC 4 cut(s) 210, 245, 287, 290
FspBI CTAG 2 cut(s) 35, 92
GlaI GCGC 2 cut(s) 285, 389
GluI GCNGC 4 cut(s) 210, 245, 287, 290
GsaI CCCAGC 1 cut(s) 283
GsuI CTGGAG 1 cut(s) 371
HaeIII GGCC 1 cut(s) 366
HapII CCGG 3 cut(s) 13, 367, 382
HhaI GCGC 2 cut(s) 286, 390
Hin1I GRCGYC 1 cut(s) 260
Hin6I GCGC 2 cut(s) 284, 388
HinP1I GCGC 2 cut(s) 284, 388
HinfI GANTC 5 cut(s) 19, 67, 200, 236, 323
HpaII CCGG 3 cut(s) 13, 367, 382
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 4 cut(s) 63, 72, 299, 328
Hpy188III TCNNGA 4 cut(s) 35, 46, 350, 410
Hpy8I GTNNAC 1 cut(s) 379
Hpy99I CGWCG 1 cut(s) 217
HpyAV CCTTC 2 cut(s) 149, 187
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4IV ACGT 1 cut(s) 260
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 253
HpyF3I CTNAG 1 cut(s) 69
HpySE526I ACGT 1 cut(s) 260
Hsp92I GRCGYC 1 cut(s) 260
HspAI GCGC 2 cut(s) 284, 388
Lsp1109I GCAGC 1 cut(s) 221
MaeI CTAG 2 cut(s) 35, 92
MaeII ACGT 1 cut(s) 260
MbiI CCGCTC 1 cut(s) 340
MhlI GDGCHC 1 cut(s) 403
MluCI AATT 1 cut(s) 132
MlyI GAGTC 3 cut(s) 61, 209, 245
MnlI CCTC 4 cut(s) 139, 190, 277, 341
MseI TTAA 1 cut(s) 135
MspI CCGG 3 cut(s) 13, 367, 382
MspR9I CCNGG 3 cut(s) 24, 318, 368
MvaI CCWGG 2 cut(s) 24, 318
MvnI CGCG 2 cut(s) 286, 390
MwoI GCNNNNNNNGC 2 cut(s) 218, 253
NciI CCSGG 1 cut(s) 368
PaeR7I CTCGAG 1 cut(s) 44
PcsI WCGNNNNNNNCGW 4 cut(s) 51, 209, 266, 303
PfeI GAWTC 2 cut(s) 19, 323
PinAI ACCGGT 1 cut(s) 381
PkrI GCNGC 4 cut(s) 211, 246, 288, 291
PleI GAGTC 3 cut(s) 61, 208, 244
PpsI GAGTC 3 cut(s) 61, 208, 244
Psp6I CCWGG 2 cut(s) 22, 316
PspFI CCCAGC 1 cut(s) 279
PspGI CCWGG 2 cut(s) 22, 316
PspPI GGNCC 1 cut(s) 364
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 4 cut(s) 210, 245, 287, 290
Sau96I GGNCC 1 cut(s) 364
ScaI AGTACT 1 cut(s) 113
SchI GAGTC 3 cut(s) 61, 209, 245
ScrFI CCNGG 3 cut(s) 24, 318, 368
SduI GDGCHC 1 cut(s) 403
SetI ASST 4 cut(s) 53, 93, 263, 333
Sfr274I CTCGAG 1 cut(s) 44
SlaI CTCGAG 1 cut(s) 44
SmlI CTYRAG 2 cut(s) 44, 143
SmoI CTYRAG 2 cut(s) 44, 143
Sse9I AATT 1 cut(s) 132
SsiI CCGC 7 cut(s) 207, 233, 244, 256, 286, 289, 340
SspMI CTAG 2 cut(s) 35, 92
StyD4I CCNGG 3 cut(s) 22, 316, 366
TaaI ACNGT 1 cut(s) 217
TaiI ACGT 1 cut(s) 263
TaqI TCGA 5 cut(s) 45, 191, 239, 269, 409
TasI AATT 1 cut(s) 132
TatI WGTACW 1 cut(s) 111
TauI GCSGC 3 cut(s) 247, 289, 292
TfiI GAWTC 2 cut(s) 19, 323
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TscAI CASTG 1 cut(s) 85
TseI GCWGC 1 cut(s) 209
TspGWI ACGGA 1 cut(s) 323
TspRI CASTG 1 cut(s) 85
XbaI TCTAGA 1 cut(s) 34
XhoI CTCGAG 1 cut(s) 44
XspI CTAG 2 cut(s) 35, 92
ZraI GACGTC 1 cut(s) 261
ZrmI AGTACT 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.