Rh6DG129900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005941
Physical Location & Seq
Reverse (-)
18344350 .. 18344805
456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG129900.1

Sequence Viewer

Length: 456 bp
ATGTTGGCTTTCCTGTTGATTTCCGGCTTCTCTCTAGAGATACTCGAGAGCTTCGTGGGTCTGATGACTCGGAGAAACACTGGGTGCGAGCTAGGGTCAGGGTTCTTGGAGTACTTGTATTTTCTGCCTAGAATTGAAGGGCTGGAGGGAGACGAAGGCGTGCCTATGGATTTGAATGTTGCTTCGGCGTCGAAGGAGGAGTCGTCCGTAGCGACGGTGGCTATTCTAGTGGCGGAGTCGAAGCAGCGCTCGCGGCGGACGTCATCCTCGATTTCTTGCTGGGCGCGGCGGCTTCTCGGGGTTGGCGTTGTTCCACCGCGGGAATCAAACCTCTGTGAGCGGGCTGTTCTGGAGCTGGTTGTGGGCCGGGTTCGGGTTCACCGGTGGTGCGAAAATGGGGCAATCGAAGAAGCAGCAATCGAGAGAGATAGAGACAGCGCTTTGTCCTTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

151

Amino Acids

16.59

Weight (kDa)

5.15

Isoelectric Point (pI)

60.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 263
AccBSI CCGCTC 1 cut(s) 340
AccII CGCG 3 cut(s) 253, 286, 319
AciI CCGC 8 cut(s) 233, 253, 256, 286, 289, 317, 319, 340
AcyI GRCGYC 2 cut(s) 188, 260
AdeI CACNNNGTG 1 cut(s) 84
AfaI GTAC 1 cut(s) 113
AfeI AGCGCT 2 cut(s) 248, 439
AfiI CCNNNNNNNGG 1 cut(s) 373
AgeI ACCGGT 1 cut(s) 381
AgsI TTSAA 2 cut(s) 137, 175
AluBI AGCT 3 cut(s) 51, 91, 355
AluI AGCT 3 cut(s) 51, 91, 355
Alw26I GTCTC 2 cut(s) 144, 426
Ama87I CYCGRG 2 cut(s) 44, 296
Aor51HI AGCGCT 2 cut(s) 248, 439
AoxI GGCC 1 cut(s) 364
ApeKI GCWGC 2 cut(s) 244, 413
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AsiGI ACCGGT 1 cut(s) 381
AspLEI GCGC 3 cut(s) 249, 286, 440
AspS9I GGNCC 1 cut(s) 364
AsuC2I CCSGG 1 cut(s) 368
AsuHPI GGTGA 1 cut(s) 371
AvaI CYCGRG 2 cut(s) 44, 296
BbvI GCAGC 2 cut(s) 256, 425
BcnI CCSGG 1 cut(s) 368
BcoDI GTCTC 2 cut(s) 144, 426
BfaI CTAG 4 cut(s) 35, 92, 129, 227
BfoI RGCGCY 2 cut(s) 250, 441
BisI GCNGC 5 cut(s) 245, 254, 287, 290, 414
BlsI GCNGC 5 cut(s) 246, 255, 288, 291, 415
BmcAI AGTACT 1 cut(s) 113
Bme1390I CCNGG 1 cut(s) 368
BmeT110I CYCGRG 2 cut(s) 44, 296
BmgT120I GGNCC 1 cut(s) 364
BmrFI CCNGG 1 cut(s) 368
BmrI ACTGGG 1 cut(s) 90
BmuI ACTGGG 1 cut(s) 90
BpmI CTGGAG 2 cut(s) 164, 371
BpuMI CCSGG 1 cut(s) 368
BsaHI GRCGYC 2 cut(s) 188, 260
BsaJI CCNNGG 2 cut(s) 317, 447
BsaWI WCCGGW 1 cut(s) 381
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 188, 218
Bsc4I CCNNNNNNNGG 1 cut(s) 373
Bse118I RCCGGY 1 cut(s) 381
Bse1I ACTGG 1 cut(s) 85
BseDI CCNNGG 2 cut(s) 317, 447
BseGI GGATG 1 cut(s) 263
BseLI CCNNNNNNNGG 1 cut(s) 373
BseNI ACTGG 1 cut(s) 85
BseRI GAGGAG 1 cut(s) 212
BseXI GCAGC 2 cut(s) 256, 425
BseYI CCCAGC 1 cut(s) 279
Bsh1236I CGCG 3 cut(s) 253, 286, 319
BshFI GGCC 1 cut(s) 366
BshTI ACCGGT 1 cut(s) 381
BsiHKCI CYCGRG 2 cut(s) 44, 296
BsiSI CCGG 3 cut(s) 24, 367, 382
BslI CCNNNNNNNGG 1 cut(s) 373
BsmAI GTCTC 2 cut(s) 144, 426
BsmBI CGTCTC 1 cut(s) 144
BsnI GGCC 1 cut(s) 366
BsoBI CYCGRG 2 cut(s) 44, 296
BspACI CCGC 8 cut(s) 233, 253, 256, 286, 289, 317, 319, 340
BspANI GGCC 1 cut(s) 366
BspFNI CGCG 3 cut(s) 253, 286, 319
BsrBI CCGCTC 1 cut(s) 340
BsrFI RCCGGY 1 cut(s) 381
BsrI ACTGG 1 cut(s) 85
BssAI RCCGGY 1 cut(s) 381
BssECI CCNNGG 2 cut(s) 317, 447
BssNI GRCGYC 2 cut(s) 188, 260
BssT1I CCWWGG 1 cut(s) 447
Bst4CI ACNGT 1 cut(s) 217
BstACI GRCGYC 2 cut(s) 188, 260
BstC8I GCNNGC 4 cut(s) 89, 161, 251, 342
BstDSI CCRYGG 1 cut(s) 317
BstF5I GGATG 1 cut(s) 263
BstFNI CGCG 3 cut(s) 253, 286, 319
BstH2I RGCGCY 2 cut(s) 250, 441
BstHHI GCGC 3 cut(s) 249, 286, 440
BstMAI GTCTC 2 cut(s) 144, 426
BstMWI GCNNNNNNNGC 3 cut(s) 218, 250, 253
BstSCI CCNGG 1 cut(s) 366
BstUI CGCG 3 cut(s) 253, 286, 319
BstV1I GCAGC 2 cut(s) 256, 425
BsuRI GGCC 1 cut(s) 366
BtgI CCRYGG 1 cut(s) 317
BtsCI GGATG 1 cut(s) 263
BtsIMutI CAGTG 1 cut(s) 78
Cac8I GCNNGC 4 cut(s) 89, 161, 251, 342
CfoI GCGC 3 cut(s) 249, 286, 440
Cfr10I RCCGGY 1 cut(s) 381
Cfr13I GGNCC 1 cut(s) 364
Cfr42I CCGCGG 1 cut(s) 320
CseI GACGC 1 cut(s) 177
Csp6I GTAC 1 cut(s) 112
CspAI ACCGGT 1 cut(s) 381
CviQI GTAC 1 cut(s) 112
DraIII CACNNNGTG 1 cut(s) 84
EciI GGCGGA 2 cut(s) 248, 271
Eco130I CCWWGG 1 cut(s) 447
Eco47III AGCGCT 2 cut(s) 248, 439
Eco88I CYCGRG 2 cut(s) 44, 296
EcoT14I CCWWGG 1 cut(s) 447
ErhI CCWWGG 1 cut(s) 447
Esp3I CGTCTC 1 cut(s) 144
FaiI YATR 1 cut(s) 167
FauI CCCGC 2 cut(s) 312, 333
Fnu4HI GCNGC 5 cut(s) 245, 254, 287, 290, 414
FokI GGATG 1 cut(s) 250
Fsp4HI GCNGC 5 cut(s) 245, 254, 287, 290, 414
FspBI CTAG 4 cut(s) 35, 92, 129, 227
GlaI GCGC 3 cut(s) 248, 285, 439
GluI GCNGC 5 cut(s) 245, 254, 287, 290, 414
GsaI CCCAGC 1 cut(s) 283
GsuI CTGGAG 2 cut(s) 164, 371
HaeII RGCGCY 2 cut(s) 250, 441
HaeIII GGCC 1 cut(s) 366
HapII CCGG 3 cut(s) 24, 367, 382
HgaI GACGC 1 cut(s) 177
HhaI GCGC 3 cut(s) 249, 286, 440
Hin1I GRCGYC 2 cut(s) 188, 260
Hin6I GCGC 3 cut(s) 247, 284, 438
HinP1I GCGC 3 cut(s) 247, 284, 438
HinfI GANTC 4 cut(s) 67, 200, 236, 323
HpaII CCGG 3 cut(s) 24, 367, 382
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 2 cut(s) 63, 72
Hpy188III TCNNGA 4 cut(s) 35, 46, 350, 421
Hpy8I GTNNAC 1 cut(s) 379
Hpy99I CGWCG 2 cut(s) 193, 217
HpyAV CCTTC 3 cut(s) 131, 149, 187
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4IV ACGT 1 cut(s) 260
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 250, 253
HpySE526I ACGT 1 cut(s) 260
Hsp92I GRCGYC 2 cut(s) 188, 260
HspAI GCGC 3 cut(s) 247, 284, 438
KspI CCGCGG 1 cut(s) 320
LmnI GCTCC 1 cut(s) 352
Lsp1109I GCAGC 2 cut(s) 256, 425
MaeI CTAG 4 cut(s) 35, 92, 129, 227
MaeII ACGT 1 cut(s) 260
MbiI CCGCTC 1 cut(s) 340
MboII GAAGA 1 cut(s) 419
MluCI AATT 1 cut(s) 132
MlyI GAGTC 3 cut(s) 61, 209, 245
MnlI CCTC 4 cut(s) 139, 190, 277, 341
MspA1I CMGCKG 1 cut(s) 319
MspI CCGG 3 cut(s) 24, 367, 382
MspR9I CCNGG 1 cut(s) 368
MvnI CGCG 3 cut(s) 253, 286, 319
MwoI GCNNNNNNNGC 3 cut(s) 218, 250, 253
NciI CCSGG 1 cut(s) 368
PaeR7I CTCGAG 1 cut(s) 44
PcsI WCGNNNNNNNCGW 5 cut(s) 51, 209, 257, 266, 303
PfeI GAWTC 1 cut(s) 323
PinAI ACCGGT 1 cut(s) 381
PkrI GCNGC 5 cut(s) 246, 255, 288, 291, 415
PleI GAGTC 3 cut(s) 61, 208, 244
PpsI GAGTC 3 cut(s) 61, 208, 244
PspFI CCCAGC 1 cut(s) 279
PspPI GGNCC 1 cut(s) 364
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
SacII CCGCGG 1 cut(s) 320
SatI GCNGC 5 cut(s) 245, 254, 287, 290, 414
Sau96I GGNCC 1 cut(s) 364
ScaI AGTACT 1 cut(s) 113
SchI GAGTC 3 cut(s) 61, 209, 245
ScrFI CCNGG 1 cut(s) 368
SetI ASST 5 cut(s) 53, 93, 263, 333, 357
Sfr274I CTCGAG 1 cut(s) 44
Sfr303I CCGCGG 1 cut(s) 320
SgrAI CRCCGGYG 1 cut(s) 381
SgrBI CCGCGG 1 cut(s) 320
SlaI CTCGAG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 1 cut(s) 132
SsiI CCGC 8 cut(s) 233, 253, 256, 286, 289, 317, 319, 340
SspMI CTAG 4 cut(s) 35, 92, 129, 227
StyD4I CCNGG 1 cut(s) 366
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 1 cut(s) 217
TaiI ACGT 1 cut(s) 263
TaqI TCGA 6 cut(s) 45, 191, 239, 269, 405, 420
TasI AATT 1 cut(s) 132
TatI WGTACW 1 cut(s) 111
TauI GCSGC 3 cut(s) 256, 289, 292
TfiI GAWTC 1 cut(s) 323
TscAI CASTG 1 cut(s) 85
TseI GCWGC 2 cut(s) 244, 413
TspGWI ACGGA 1 cut(s) 196
TspRI CASTG 1 cut(s) 85
XbaI TCTAGA 1 cut(s) 34
XhoI CTCGAG 1 cut(s) 44
XspI CTAG 4 cut(s) 35, 92, 129, 227
ZraI GACGTC 1 cut(s) 261
ZrmI AGTACT 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.