Rroxscaffold_1G00041370

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010603
Physical Location & Seq
Forward (+)
59243457 .. 59273537
30081 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00041370.1

Sequence Viewer

Length: 1074 bp
ATGAAGAAATTAGTTTCAAAACTGATCCCAAGAGTTGACCGATTTGGACAAGAATATCAACCCTTAAAAGCTTTGGGCATTTTGGGCCAAATTCCCGAAACGGATGAGTTAAATAGAAGATTTGGTATGGATGTTAACACAAGTGGTGACCGGATTTCACTCGTACCAACAACTTCCAATACATTTCCAATTAAGGGTCACCAGTGGTGGCAAAGCTCGCTCAATTCCTCCAAAGAAATCCATTACATATATTCGATTCAAGCAATAACAATTCCCCGAGCATTAATTCAAGAAAATAAACGCATGCATCATTATTTAAAACGAAAGTCCACTCACAGTACTATTGGGCGACCACGCAAACGAGTTCCTTCATCTAGCCGTAGCGCGCGACATTGCCCTGTAGGTTTCAATTGGGTGGGGCAACATTCCAGTTCCAATCCTCTATTCCATCTCCGTGGCATGGTCGTTGTTTTCGGACGTGCGCTTGTCAAGCCTAGGAAGGAGAAGAGGGCAGTGGTCATCGGAGATACCGATTCTAGGTCTTCGGTGAGTGTAGATGATATCTTGGCTGTGGTATCGGTGGATGATTCCGTGCGTAAGAAGAGGGGCACCGCCATCCCCAACCACAAGGACCGCCGCCTCATCTTCAACATCTTCGATTTCCACTCCTATGGCGACCTCGACAACATATCTGCTTTGTGTGGGTTTAATGGCCTCTTTGTGTCTCACGGCGTCCCTTCCACCACTCTCCTAGATGTTAAGGATGCTCTCCTCTCAATCTCAACGGTGTTGAAACACGGGAATAATATACATTGCTCAGCTTTAGAGCTTGCTTCTGTGAGTACTTTGGCCTCCAAGTTCCTCTCAGCTCAACTGATCTCAAACTGGGCATCTCTCAAGTTTGTAGCGAACCCCCAGCCATCGACCACAGACCACCCGCGGCCCTACCGGAACCCTAATCTCAGATTCATCAACGGAGGTGCATTTGAACAAAACCCTAATTTCAGCAGCGGCGGTGCATTTGAAGAAAACCATGATAGCCCATCTAACAATCGTCTGAGAAGGACCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

357

Amino Acids

39.95

Weight (kDa)

10.25

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 608
AccII CGCG 3 cut(s) 386, 388, 940
AciI CCGC 7 cut(s) 612, 634, 637, 938, 940, 1011, 1014
AclWI GGATC 1 cut(s) 19
AcsI RAATTY 1 cut(s) 90
AcyI GRCGYC 1 cut(s) 732
AfaI GTAC 3 cut(s) 165, 340, 844
AfiI CCNNNNNNNGG 3 cut(s) 194, 460, 537
AgsI TTSAA 8 cut(s) 18, 260, 290, 409, 649, 793, 989, 1025
AjiI CACGTC 1 cut(s) 479
AjuI GAANNNNNNNTTGG 2 cut(s) 428, 460
AluBI AGCT 5 cut(s) 71, 216, 821, 829, 869
AluI AGCT 5 cut(s) 71, 216, 821, 829, 869
Alw26I GTCTC 1 cut(s) 729
AlwI GGATC 1 cut(s) 19
Ama87I CYCGRG 1 cut(s) 276
AoxI GGCC 4 cut(s) 85, 712, 849, 941
ApeKI GCWGC 1 cut(s) 1008
ApoI RAATTY 1 cut(s) 90
AseI ATTAAT 1 cut(s) 284
AspA2I CCTAGG 1 cut(s) 494
AspLEI GCGC 3 cut(s) 386, 388, 484
AspS9I GGNCC 4 cut(s) 85, 631, 942, 1065
AsuHPI GGTGA 3 cut(s) 158, 191, 559
AvaI CYCGRG 1 cut(s) 276
AvaII GGWCC 2 cut(s) 631, 1065
AvrII CCTAGG 1 cut(s) 494
BaeGI GKGCMC 1 cut(s) 611
BanI GGYRCC 1 cut(s) 608
BbsI GAAGAC 1 cut(s) 534
BbvI GCAGC 1 cut(s) 1020
BccI CCATC 4 cut(s) 456, 623, 928, 1051
BceAI ACGGC 2 cut(s) 363, 745
BcoDI GTCTC 1 cut(s) 729
BfaI CTAG 4 cut(s) 375, 495, 537, 752
BfmI CTRYAG 1 cut(s) 399
BisI GCNGC 4 cut(s) 637, 941, 1009, 1012
BlnI CCTAGG 1 cut(s) 494
BlpI GCTNAGC 1 cut(s) 817
BlsI GCNGC 4 cut(s) 638, 942, 1010, 1013
BmcAI AGTACT 2 cut(s) 340, 844
Bme18I GGWCC 2 cut(s) 631, 1065
BmeT110I CYCGRG 1 cut(s) 276
BmgBI CACGTC 1 cut(s) 479
BmgT120I GGNCC 4 cut(s) 85, 631, 942, 1065
BmiI GGNNCC 3 cut(s) 610, 953, 1067
BmrI ACTGGG 1 cut(s) 895
BmsI GCATC 3 cut(s) 316, 754, 899
BmuI ACTGGG 1 cut(s) 895
BpiI GAAGAC 1 cut(s) 534
Bpu1102I GCTNAGC 1 cut(s) 817
BpuEI CTTGAG 1 cut(s) 881
BsaHI GRCGYC 1 cut(s) 732
BsaJI CCNNGG 3 cut(s) 454, 494, 938
BsaWI WCCGGW 2 cut(s) 150, 948
Bsc4I CCNNNNNNNGG 3 cut(s) 194, 460, 537
Bse1I ACTGG 3 cut(s) 202, 429, 890
Bse3DI GCAATG 2 cut(s) 391, 811
BseDI CCNNGG 3 cut(s) 454, 494, 938
BseGI GGATG 5 cut(s) 109, 136, 589, 615, 769
BseLI CCNNNNNNNGG 3 cut(s) 194, 460, 537
BseMI GCAATG 2 cut(s) 391, 811
BseMII CTCAG 4 cut(s) 831, 879, 976, 1049
BseNI ACTGG 3 cut(s) 202, 429, 890
BsePI GCGCGC 1 cut(s) 384
BseRI GAGGAG 1 cut(s) 761
BseSI GKGCMC 1 cut(s) 611
BseXI GCAGC 1 cut(s) 1020
BseYI CCCAGC 1 cut(s) 915
Bsh1236I CGCG 3 cut(s) 386, 388, 940
BshFI GGCC 4 cut(s) 87, 714, 851, 943
BshNI GGYRCC 1 cut(s) 608
BsiHKCI CYCGRG 1 cut(s) 276
BsiSI CCGG 2 cut(s) 151, 949
BslFI GGGAC 1 cut(s) 719
BslI CCNNNNNNNGG 3 cut(s) 194, 460, 537
BsmAI GTCTC 1 cut(s) 729
BsmFI GGGAC 1 cut(s) 719
BsnI GGCC 4 cut(s) 87, 714, 851, 943
BsoBI CYCGRG 1 cut(s) 276
Bsp1286I GDGCHC 1 cut(s) 611
Bsp143I GATC 2 cut(s) 24, 876
Bsp1720I GCTNAGC 1 cut(s) 817
BspACI CCGC 7 cut(s) 612, 634, 637, 938, 940, 1011, 1014
BspANI GGCC 4 cut(s) 87, 714, 851, 943
BspCNI CTCAG 4 cut(s) 830, 878, 975, 1050
BspFNI CGCG 3 cut(s) 386, 388, 940
BspLI GGNNCC 3 cut(s) 610, 953, 1067
BspPI GGATC 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 608
BsrDI GCAATG 2 cut(s) 391, 811
BsrI ACTGG 3 cut(s) 202, 429, 890
BssECI CCNNGG 3 cut(s) 454, 494, 938
BssHII GCGCGC 1 cut(s) 384
BssMI GATC 2 cut(s) 24, 876
BssNI GRCGYC 1 cut(s) 732
BssT1I CCWWGG 1 cut(s) 494
Bst4CI ACNGT 2 cut(s) 338, 787
Bst6I CTCTTC 2 cut(s) 500, 596
BstACI GRCGYC 1 cut(s) 732
BstC8I GCNNGC 4 cut(s) 218, 305, 386, 831
BstDEI CTNAG 4 cut(s) 817, 865, 962, 1058
BstDSI CCRYGG 2 cut(s) 454, 938
BstEII GGTNACC 2 cut(s) 146, 197
BstF5I GGATG 5 cut(s) 109, 136, 589, 615, 769
BstFNI CGCG 3 cut(s) 386, 388, 940
BstHHI GCGC 3 cut(s) 386, 388, 484
BstKTI GATC 2 cut(s) 27, 879
BstMAI GTCTC 1 cut(s) 729
BstMBI GATC 2 cut(s) 24, 876
BstMWI GCNNNNNNNGC 3 cut(s) 84, 217, 490
BstNSI RCATGY 1 cut(s) 307
BstPI GGTNACC 2 cut(s) 146, 197
BstSFI CTRYAG 1 cut(s) 399
BstSLI GKGCMC 1 cut(s) 611
BstUI CGCG 3 cut(s) 386, 388, 940
BstV1I GCAGC 1 cut(s) 1020
BstV2I GAAGAC 1 cut(s) 534
BstXI CCANNNNNNTGG 2 cut(s) 455, 671
BsuRI GGCC 4 cut(s) 87, 714, 851, 943
BtgI CCRYGG 2 cut(s) 454, 938
BtrI CACGTC 1 cut(s) 479
BtsCI GGATG 5 cut(s) 109, 136, 589, 615, 769
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 2 cut(s) 209, 519
Cac8I GCNNGC 4 cut(s) 218, 305, 386, 831
CfoI GCGC 3 cut(s) 386, 388, 484
Cfr13I GGNCC 4 cut(s) 85, 631, 942, 1065
Cfr42I CCGCGG 1 cut(s) 941
CseI GACGC 1 cut(s) 721
Csp6I GTAC 3 cut(s) 164, 339, 843
CviAII CATG 3 cut(s) 304, 460, 1034
CviQI GTAC 3 cut(s) 164, 339, 843
DdeI CTNAG 4 cut(s) 817, 865, 962, 1058
DpnI GATC 2 cut(s) 26, 878
DpnII GATC 2 cut(s) 24, 876
DraI TTTAAA 1 cut(s) 318
Eam1104I CTCTTC 2 cut(s) 500, 596
EarI CTCTTC 2 cut(s) 500, 596
Eco130I CCWWGG 1 cut(s) 494
Eco32I GATATC 1 cut(s) 562
Eco47I GGWCC 2 cut(s) 631, 1065
Eco88I CYCGRG 1 cut(s) 276
Eco91I GGTNACC 2 cut(s) 146, 197
EcoO109I RGGNCCY 1 cut(s) 1065
EcoO65I GGTNACC 2 cut(s) 146, 197
EcoRV GATATC 1 cut(s) 562
EcoT14I CCWWGG 1 cut(s) 494
EcoT22I ATGCAT 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 494
FaeI CATG 3 cut(s) 307, 463, 1037
FaiI YATR 9 cut(s) 128, 248, 250, 305, 461, 672, 689, 809, 1035
FaqI GGGAC 1 cut(s) 719
FatI CATG 3 cut(s) 303, 459, 1033
FauI CCCGC 1 cut(s) 945
Fnu4HI GCNGC 4 cut(s) 637, 941, 1009, 1012
FokI GGATG 5 cut(s) 116, 143, 596, 602, 776
Fsp4HI GCNGC 4 cut(s) 637, 941, 1009, 1012
FspBI CTAG 4 cut(s) 375, 495, 537, 752
GlaI GCGC 3 cut(s) 385, 387, 483
GluI GCNGC 4 cut(s) 637, 941, 1009, 1012
GsaI CCCAGC 1 cut(s) 919
HaeIII GGCC 4 cut(s) 87, 714, 851, 943
HapII CCGG 2 cut(s) 151, 949
HgaI GACGC 1 cut(s) 721
HhaI GCGC 3 cut(s) 386, 388, 484
Hin1I GRCGYC 1 cut(s) 732
Hin1II CATG 3 cut(s) 307, 463, 1037
Hin6I GCGC 3 cut(s) 384, 386, 482
HinP1I GCGC 3 cut(s) 384, 386, 482
HincII GTYRAC 2 cut(s) 37, 136
HindII GTYRAC 2 cut(s) 37, 136
HindIII AAGCTT 1 cut(s) 69
HinfI GANTC 4 cut(s) 256, 533, 587, 966
HpaI GTTAAC 1 cut(s) 136
HpaII CCGG 2 cut(s) 151, 949
HphI GGTGA 3 cut(s) 158, 191, 559
Hpy166II GTNNAC 3 cut(s) 37, 136, 330
Hpy188I TCNGA 4 cut(s) 476, 524, 965, 1059
Hpy188III TCNNGA 2 cut(s) 95, 290
Hpy8I GTNNAC 3 cut(s) 37, 136, 330
HpyAV CCTTC 4 cut(s) 378, 493, 747, 1056
HpyCH4III ACNGT 2 cut(s) 338, 787
HpyCH4IV ACGT 1 cut(s) 478
HpyCH4V TGCA 3 cut(s) 307, 983, 1019
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 217, 490
HpyF3I CTNAG 4 cut(s) 817, 865, 962, 1058
HpySE526I ACGT 1 cut(s) 478
Hsp92I GRCGYC 1 cut(s) 732
Hsp92II CATG 3 cut(s) 307, 463, 1037
HspAI GCGC 3 cut(s) 384, 386, 482
KspAI GTTAAC 1 cut(s) 136
KspI CCGCGG 1 cut(s) 941
Kzo9I GATC 2 cut(s) 24, 876
LpnPI CCDG 7 cut(s) 164, 215, 411, 442, 871, 929, 962
Lsp1109I GCAGC 1 cut(s) 1020
LweI GCATC 3 cut(s) 316, 754, 899
MaeI CTAG 4 cut(s) 375, 495, 537, 752
MaeII ACGT 1 cut(s) 478
MaeIII GTNAC 2 cut(s) 146, 197
MalI GATC 2 cut(s) 26, 878
MboI GATC 2 cut(s) 24, 876
MboII GAAGA 8 cut(s) 16, 129, 517, 534, 613, 637, 646, 1037
MfeI CAATTG 1 cut(s) 409
MhlI GDGCHC 1 cut(s) 611
MluCI AATT 8 cut(s) 8, 90, 189, 223, 270, 285, 409, 1000
Mph1103I ATGCAT 1 cut(s) 309
MseI TTAA 8 cut(s) 65, 110, 135, 192, 284, 317, 708, 759
MslI CAYNNNNRTG 2 cut(s) 453, 669
MspA1I CMGCKG 2 cut(s) 940, 1011
MspI CCGG 2 cut(s) 151, 949
MunI CAATTG 1 cut(s) 409
MvnI CGCG 3 cut(s) 386, 388, 940
MwoI GCNNNNNNNGC 3 cut(s) 84, 217, 490
NdeII GATC 2 cut(s) 24, 876
NlaIII CATG 3 cut(s) 307, 463, 1037
NlaIV GGNNCC 3 cut(s) 610, 953, 1067
NmuCI GTSAC 2 cut(s) 146, 197
NsiI ATGCAT 1 cut(s) 309
NspI RCATGY 1 cut(s) 307
PaeI GCATGC 1 cut(s) 307
PauI GCGCGC 1 cut(s) 384
PcsI WCGNNNNNNNCGW 1 cut(s) 528
PfeI GAWTC 4 cut(s) 256, 533, 587, 966
PkrI GCNGC 4 cut(s) 638, 942, 1010, 1013
PpuMI RGGWCCY 1 cut(s) 1065
PshBI ATTAAT 1 cut(s) 284
Psp5II RGGWCCY 1 cut(s) 1065
PspEI GGTNACC 2 cut(s) 146, 197
PspFI CCCAGC 1 cut(s) 915
PspN4I GGNNCC 3 cut(s) 610, 953, 1067
PspPI GGNCC 4 cut(s) 85, 631, 942, 1065
PspPPI RGGWCCY 1 cut(s) 1065
PteI GCGCGC 1 cut(s) 384
RsaI GTAC 3 cut(s) 165, 340, 844
RsaNI GTAC 3 cut(s) 164, 339, 843
RseI CAYNNNNRTG 2 cut(s) 453, 669
SacII CCGCGG 1 cut(s) 941
SaqAI TTAA 8 cut(s) 65, 110, 135, 192, 284, 317, 708, 759
SatI GCNGC 4 cut(s) 637, 941, 1009, 1012
Sau3AI GATC 2 cut(s) 24, 876
Sau96I GGNCC 4 cut(s) 85, 631, 942, 1065
ScaI AGTACT 2 cut(s) 340, 844
SduI GDGCHC 1 cut(s) 611
SfaNI GCATC 3 cut(s) 316, 754, 899
SfcI CTRYAG 1 cut(s) 399
Sfr303I CCGCGG 1 cut(s) 941
SgrBI CCGCGG 1 cut(s) 941
SinI GGWCC 2 cut(s) 631, 1065
SmiMI CAYNNNNRTG 2 cut(s) 453, 669
SmlI CTYRAG 1 cut(s) 896
SmoI CTYRAG 1 cut(s) 896
SphI GCATGC 1 cut(s) 307
Sse9I AATT 8 cut(s) 8, 90, 189, 223, 270, 285, 409, 1000
SsiI CCGC 7 cut(s) 612, 634, 637, 938, 940, 1011, 1014
SspMI CTAG 4 cut(s) 375, 495, 537, 752
StyI CCWWGG 1 cut(s) 494
TaaI ACNGT 2 cut(s) 338, 787
TaiI ACGT 1 cut(s) 481
TaqI TCGA 4 cut(s) 254, 657, 681, 923
TaqII GACCGA 1 cut(s) 54
TasI AATT 8 cut(s) 8, 90, 189, 223, 270, 285, 409, 1000
TatI WGTACW 2 cut(s) 338, 842
TauI GCSGC 3 cut(s) 639, 943, 1014
TfiI GAWTC 4 cut(s) 256, 533, 587, 966
Tru1I TTAA 8 cut(s) 65, 110, 135, 192, 284, 317, 708, 759
Tru9I TTAA 8 cut(s) 65, 110, 135, 192, 284, 317, 708, 759
TscAI CASTG 2 cut(s) 209, 519
TseFI GTSAC 2 cut(s) 146, 197
TseI GCWGC 1 cut(s) 1008
Tsp45I GTSAC 2 cut(s) 146, 197
TspDTI ATGAA 3 cut(s) 17, 360, 958
TspGWI ACGGA 4 cut(s) 116, 443, 580, 990
TspRI CASTG 2 cut(s) 209, 519
VpaK11BI GGWCC 2 cut(s) 631, 1065
VspI ATTAAT 1 cut(s) 284
XapI RAATTY 1 cut(s) 90
XceI RCATGY 1 cut(s) 307
XmaJI CCTAGG 1 cut(s) 494
XspI CTAG 4 cut(s) 375, 495, 537, 752
ZrmI AGTACT 2 cut(s) 340, 844
Zsp2I ATGCAT 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.