Rorug05G0037800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010603
Physical Location & Seq
Reverse (-)
2877977 .. 2916654
38678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0037800.1

Sequence Viewer

Length: 2691 bp
ATGAGTTTCAGTATCGTCGAAGCGTTCATCCAGATATTGCTTCTCTTCCTTCTTCTTCCCTCTGGCATTTGCCAGCTTTCCCTTAGCGCCCTTACGCCAAACCGTCCCATCAGAGACGGCGACGTTTTAGTCTCTAGCAGGAAAATCTTTGCACTAGGGTTCTTCAGCCCTGGAAATTCCAGGCATCGCTATGTTGGAGTTTGGTATAACAACAATGTTCCGAACCAAACCATCGTCTGGGTTGCAAACAGAGACAACCCAGTTAATGATTCCTCTGGAGTGCTATCGATCAATGGTGATGGAGGCCTTGTCATACACGGAAAAGACCGAAGTACCCCTCTTTGGTCCGCAAACGTTACCCTCTCTTCTCCTAACAATTCCATAGCCAATCTTTTGGATACAGGAAATCTTGTTTTGCTTGAGAGTGAGAGTAGCCAAAGAGTTGTGTGGGAAGGATTTGATTATCCCACAGATACACTGCTTGCCTCTATGAAGATTGGGCTGAACCGGCGGTCCGGGTGGGACCGGTTCCTAACATCTTGGAAGTCGACAGATGACCCGGGAACCGGGAGCTGTACATTGAGGATAGACGCGAGGGGGTCTCCGCAGATGTTTGTGTACAAGGGTGGGGCCCCATTTTGGCGGGCTGGACCTTGGGTCGGGGAGAGATGGAGCGGTGTACCCGAACTTACCACTACCTTTGGACTCCACAATGTAAGTTTTGTCAACAATAAAGACGAGATATCGCTAATGTATGCTGTTTTCAATGACTCATTGATCGCAAGGGGGGTGCTAGATGAATCAGGAATATATCACCGGCTCATGTGGAATGATCAGGAAAACCAATGGATCGAATTGGTCTCCGACCCGAATGAGTCGTGTGACAACTATGGATGGTGCGGTCTAAATACTAACTGTGACCCATACAAGTACTCTGAGTTCACTTGCACGTGCCTACCCGGGTTTGAACCCAAATCGCCTCGTGACTGGTATGTAAGAATTCGGGCGGGTGGGTGCATTAGGAAAGCGGGAGCGTCCACATGTCGGAACGGAGAAGGGTTCGTGAAGGTGGCGCGTGTAAAAGTTCCAGACTCGTCGACGGCGCATGTGAACATGAGTATGAGTTTGAAAGGGTGCAAACAAGAGTGCTTGATGGATTGTTCTTGCACAGCTTACGCGAGTGCGGATGAGCGAAGAGGTGGGACCGGGTGCGTGAGATGGCACGGGGACTTGATGGACACGAGAACTTACTCCAATACTGGTCAAGATTTATACATACGAGTTGATGCAACTACCTTAGCTCAATATGCAGAGTCAAGTGGTTCTCTTAGCAAGAAGGCAACCCTGGCGATTTCATTAGGATCTGTTACAGTTTTCCTTCTCTTACTTACCCTTTATTGGTTGGTAATGATGAAGTTGAAAGGCAAGCGAAGACGAAATAAATTTTCATTTATACCTACCGCCGGGTCAACCTACTTCGAAGAATCTTCTGGTGGAACAGCTTTTGATGATAGTAAAATAAACTCAGAGTTAGCATTCTTTCATCTAAAAACCATAGCAACCGCCACAAACAATTTCTCAATTGAAAACAAGCTTGGAGAGGGAGGTTTTGGCTCCGTTTATAAGGGCATACTTTACGATGGAAAGGAAATAGCAGTAAAAAGACTATCCAAGTTTTCTGGCCAAGGAGTTGAAGAGTTTAAGAATGAAGTTCTTCTAATTGCAAAACTCCAACACAGAAACCTTGTCAGGATTCTAGGTTATTGTGTTGAAGATGAAGAGAAGATTCTAATCTATGAATACTTACCAAACAAAAGTTTGGACTCTTTCATCTTTAATGAAACAAAGAGAGCACTTTTAAATTGGACAAAACGTTTCAAGATTATCTTTGGGATTGCTAGAGGGGCATTATATCTTCACGAAGATTCGAGATTAAGAATTATCCATAGAGATCTAAAGGCCAGCAATGTTCTATTGGATAATTCTTTGAACCCAAAAATTGCAGATTTTGGCATGGCTAGAATATTTAGAGGGGAGCAAAGTGAAGCGAATACCAATCGTGTGGTTGGAACATATGGTTATATGTCCCCGGAATATGCAATGGAAGGACTGTTTTCAATAAAGTCTGATGTGTATAGTTTTGGTGTTTTACTGCTAGAAATCATCACTGGCAAAAAGAATGCTGGCTATTACCATGAGGAGTATCCTAATTCAAATTTGGTTGGACATGTTTGGAACTTGTGGCGAGAAAGTAATGCCTTGGAAGTCGTTGATTCATCTCTGGGTGAATCATACCCCACGAATGAAGTTCTGAGATGTATCCAAATTGCTCTCTTGTGTGTGCAAGAGTATGCAATTGACCGGCCAACCATGTCGGCAGTTCTTAACATGTTAGGTAATGATGCAACACTTCCTTCACCTAGACAACCTGCATTTCTATTGCAGAGAAGTGCCACTAGTACTGGAGACTCATCAACCCAAGATGGAGCTAATTCTGTAAATGATGTGACATTCACAGTCATCTTCCTCATGAACTGGCCATGCGGAAATGGCAAAGGATTTGTGAAGGTGGAACTTGTAAATGTACCAGACTCGTCTAGGGCAGGTTTGAATATAAACTTGAACTTGAAAGCATGCGAGCAAGAGCTAGTGCTTGAGAAACTGTTCTTGCATGGCATGTTCGAGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

896

Amino Acids

99.78

Weight (kDa)

6.7

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 77 - 182 1.1e-34 D-mannose binding lectin
S_locus_glycop PF00954 214 - 326 5e-25 S-locus glycoprotein domain
PAN_2 PF08276 348 - 414 7.2e-22 PAN-like domain
PK_Tyr_Ser-Thr PF07714 527 - 798 3.6e-48 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 527 - 729 4.8e-42 Protein kinase domain
DUF3403 PF11883 800 - 841 9e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1623
AasI GACNNNNNNGTC 1 cut(s) 128
Acc36I ACCTGC 2 cut(s) 2437, 2594
AccB7I CCANNNNNTGG 1 cut(s) 237
AccBSI CCGCTC 1 cut(s) 675
AccI GTMKAC 2 cut(s) 548, 1097
AccII CGCG 3 cut(s) 593, 1075, 1178
AclI AACGTT 2 cut(s) 354, 1873
AclWI GGATC 2 cut(s) 857, 1369
AcoI YGGCCR 3 cut(s) 1681, 2363, 2537
AcsI RAATTY 4 cut(s) 175, 999, 1442, 2215
AcuI CTGAAG 1 cut(s) 148
AcvI CACGTG 1 cut(s) 951
AfaI GTAC 7 cut(s) 334, 577, 620, 681, 932, 2461, 2586
AfiI CCNNNNNNNGG 8 cut(s) 237, 342, 566, 639, 659, 1044, 1398, 1463
AflIII ACRYGT 3 cut(s) 1040, 2227, 2388
AgeI ACCGGT 1 cut(s) 525
AhdI GACNNNNNGTC 1 cut(s) 656
AhlI ACTAGT 1 cut(s) 2456
AjnI CCWGG 3 cut(s) 169, 179, 1344
AjuI GAANNNNNNNTTGG 2 cut(s) 1578, 1610
AloI GAACNNNNNNTCC 2 cut(s) 497, 529
AluBI AGCT 8 cut(s) 76, 573, 1172, 1301, 1502, 1594, 2489, 2647
AluI AGCT 8 cut(s) 76, 573, 1172, 1301, 1502, 1594, 2489, 2647
Alw21I GWGCWC 1 cut(s) 1855
Alw26I GTCTC 6 cut(s) 108, 136, 246, 606, 865, 2460
AlwI GGATC 2 cut(s) 857, 1369
Ama87I CYCGRG 2 cut(s) 559, 959
AoxI GGCC 6 cut(s) 304, 630, 1681, 1959, 2363, 2537
ApaI GGGCCC 1 cut(s) 634
ApoI RAATTY 4 cut(s) 175, 999, 1442, 2215
AsiGI ACCGGT 1 cut(s) 525
Asp700I GAANNNNTTC 2 cut(s) 1713, 2663
AspLEI GCGC 3 cut(s) 89, 1075, 1105
AspS9I GGNCC 7 cut(s) 345, 513, 523, 630, 631, 650, 1203
AsuC2I CCSGG 9 cut(s) 517, 560, 561, 568, 960, 961, 1207, 1465, 2090
AsuHPI GGTGA 4 cut(s) 308, 806, 2297, 2409
AsuII TTCGAA 1 cut(s) 1479
AvaI CYCGRG 2 cut(s) 559, 959
AvaII GGWCC 5 cut(s) 345, 513, 523, 650, 1203
BaeGI GKGCMC 1 cut(s) 634
BaeI ACNNNNGTAYC 2 cut(s) 316, 349
BalI TGGCCA 2 cut(s) 1683, 2539
BanII GRGCYC 1 cut(s) 634
BauI CACGAG 2 cut(s) 981, 1240
BbrPI CACGTG 1 cut(s) 951
BbsI GAAGAC 1 cut(s) 1438
Bbv12I GWGCWC 1 cut(s) 1855
BceAI ACGGC 2 cut(s) 133, 1116
BciT130I CCWGG 3 cut(s) 171, 181, 1346
BciVI GTATCC 3 cut(s) 391, 2214, 2330
BclI TGATCA 1 cut(s) 832
BcnI CCSGG 9 cut(s) 517, 560, 561, 568, 960, 961, 1207, 1465, 2090
BcoDI GTCTC 6 cut(s) 108, 136, 246, 606, 865, 2460
BcuI ACTAGT 1 cut(s) 2456
BfoI RGCGCY 1 cut(s) 90
BfuAI ACCTGC 2 cut(s) 2437, 2594
BfuI GTATCC 3 cut(s) 391, 2214, 2330
BglII AGATCT 1 cut(s) 1951
BmcAI AGTACT 2 cut(s) 932, 2461
Bme18I GGWCC 5 cut(s) 345, 513, 523, 650, 1203
BmeRI GACNNNNNGTC 1 cut(s) 656
BmeT110I CYCGRG 2 cut(s) 559, 959
BmgT120I GGNCC 7 cut(s) 345, 513, 523, 630, 631, 650, 1203
BmiI GGNNCC 8 cut(s) 524, 530, 565, 631, 632, 633, 1204, 1615
BmrI ACTGGG 1 cut(s) 254
BmsI GCATC 3 cut(s) 193, 1276, 2392
BmuI ACTGGG 1 cut(s) 254
BpiI GAAGAC 1 cut(s) 1438
BplI GAGNNNNNCTC 2 cut(s) 586, 618
BpmI CTGGAG 2 cut(s) 297, 2484
Bpu10I CCTNAGC 2 cut(s) 83, 1297
Bpu14I TTCGAA 1 cut(s) 1479
BpuEI CTTGAG 2 cut(s) 440, 2675
BpuMI CCSGG 9 cut(s) 517, 560, 561, 568, 960, 961, 1207, 1465, 2090
Bsa29I ATCGAT 1 cut(s) 287
BsaAI YACGTR 1 cut(s) 951
BsaBI GATNNNNATC 1 cut(s) 1790
BsaI GGTCTC 2 cut(s) 606, 865
BsaJI CCNNGG 8 cut(s) 169, 559, 653, 959, 1344, 1684, 2088, 2259
BsaWI WCCGGW 1 cut(s) 525
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 8 cut(s) 237, 342, 566, 639, 659, 1044, 1398, 1463
Bse118I RCCGGY 4 cut(s) 507, 525, 816, 2361
Bse1I ACTGG 6 cut(s) 260, 992, 1264, 2173, 2467, 2540
Bse3DI GCAATG 2 cut(s) 1972, 2106
Bse8I GATNNNNATC 1 cut(s) 1790
BseBI CCWGG 3 cut(s) 171, 181, 1346
BseCI ATCGAT 1 cut(s) 287
BseDI CCNNGG 8 cut(s) 169, 559, 653, 959, 1344, 1684, 2088, 2259
BseGI GGATG 3 cut(s) 27, 899, 1192
BseJI GATNNNNATC 1 cut(s) 1790
BseLI CCNNNNNNNGG 8 cut(s) 237, 342, 566, 639, 659, 1044, 1398, 1463
BseMI GCAATG 2 cut(s) 1972, 2106
BseMII CTCAG 3 cut(s) 927, 1539, 2303
BseNI ACTGG 6 cut(s) 260, 992, 1264, 2173, 2467, 2540
BseRI GAGGAG 1 cut(s) 2213
BseSI GKGCMC 1 cut(s) 634
Bsh1236I CGCG 3 cut(s) 593, 1075, 1178
BshFI GGCC 6 cut(s) 306, 632, 1683, 1961, 2365, 2539
BshTI ACCGGT 1 cut(s) 525
BshVI ATCGAT 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 1855
BsiHKCI CYCGRG 2 cut(s) 559, 959
BslFI GGGAC 5 cut(s) 90, 536, 1216, 1241, 2071
BslI CCNNNNNNNGG 8 cut(s) 237, 342, 566, 639, 659, 1044, 1398, 1463
BsmAI GTCTC 6 cut(s) 108, 136, 246, 606, 865, 2460
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 5 cut(s) 90, 536, 1216, 1241, 2071
BsmI GAATGC 2 cut(s) 1535, 2185
BsnI GGCC 6 cut(s) 306, 632, 1683, 1961, 2365, 2539
Bso31I GGTCTC 2 cut(s) 606, 865
BsoBI CYCGRG 2 cut(s) 559, 959
Bsp119I TTCGAA 1 cut(s) 1479
Bsp120I GGGCCC 1 cut(s) 630
Bsp1286I GDGCHC 2 cut(s) 634, 1855
Bsp1407I TGTACA 2 cut(s) 575, 618
Bsp143I GATC 6 cut(s) 288, 777, 832, 849, 1361, 1951
BspANI GGCC 6 cut(s) 306, 632, 1683, 1961, 2365, 2539
BspCNI CTCAG 3 cut(s) 928, 1538, 2304
BspDI ATCGAT 1 cut(s) 287
BspFNI CGCG 3 cut(s) 593, 1075, 1178
BspHI TCATGA 1 cut(s) 2529
BspLI GGNNCC 8 cut(s) 524, 530, 565, 631, 632, 633, 1204, 1615
BspMI ACCTGC 2 cut(s) 2437, 2594
BspPI GGATC 2 cut(s) 857, 1369
BspT104I TTCGAA 1 cut(s) 1479
BspTNI GGTCTC 2 cut(s) 606, 865
BsrBI CCGCTC 1 cut(s) 675
BsrDI GCAATG 2 cut(s) 1972, 2106
BsrFI RCCGGY 4 cut(s) 507, 525, 816, 2361
BsrGI TGTACA 2 cut(s) 575, 618
BsrI ACTGG 6 cut(s) 260, 992, 1264, 2173, 2467, 2540
BssAI RCCGGY 4 cut(s) 507, 525, 816, 2361
BssECI CCNNGG 8 cut(s) 169, 559, 653, 959, 1344, 1684, 2088, 2259
BssMI GATC 6 cut(s) 288, 777, 832, 849, 1361, 1951
BssSI CACGAG 2 cut(s) 981, 1240
BssT1I CCWWGG 3 cut(s) 653, 1684, 2259
Bst2BI CACGAG 2 cut(s) 981, 1240
Bst2UI CCWGG 3 cut(s) 171, 181, 1346
Bst4CI ACNGT 6 cut(s) 104, 917, 1372, 2112, 2518, 2664
Bst6I CTCTTC 5 cut(s) 50, 370, 1189, 1689, 1773
BstAUI TGTACA 2 cut(s) 575, 618
BstBAI YACGTR 1 cut(s) 951
BstBI TTCGAA 1 cut(s) 1479
BstC8I GCNNGC 8 cut(s) 74, 483, 645, 1427, 1963, 2185, 2635, 2639
BstDEI CTNAG 6 cut(s) 83, 936, 1297, 1328, 1525, 2312
BstF5I GGATG 3 cut(s) 27, 899, 1192
BstFNI CGCG 3 cut(s) 593, 1075, 1178
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 3 cut(s) 89, 1075, 1105
BstKTI GATC 6 cut(s) 291, 780, 835, 852, 1364, 1954
BstMAI GTCTC 6 cut(s) 108, 136, 246, 606, 865, 2460
BstMBI GATC 6 cut(s) 288, 777, 832, 849, 1361, 1951
BstMWI GCNNNNNNNGC 5 cut(s) 508, 1307, 1346, 1904, 2550
BstNI CCWGG 3 cut(s) 171, 181, 1346
BstNSI RCATGY 6 cut(s) 1044, 1109, 2231, 2392, 2637, 2680
BstSLI GKGCMC 1 cut(s) 634
BstUI CGCG 3 cut(s) 593, 1075, 1178
BstV2I GAAGAC 1 cut(s) 1438
BstX2I RGATCY 2 cut(s) 1361, 1951
BstXI CCANNNNNNTGG 2 cut(s) 394, 2062
BstYI RGATCY 2 cut(s) 1361, 1951
Bsu15I ATCGAT 1 cut(s) 287
BsuI GTATCC 3 cut(s) 391, 2214, 2330
BsuRI GGCC 6 cut(s) 306, 632, 1683, 1961, 2365, 2539
BsuTUI ATCGAT 1 cut(s) 287
BtgZI GCGATG 1 cut(s) 170
BtsCI GGATG 3 cut(s) 27, 899, 1192
BtsI GCAGTG 1 cut(s) 476
BtsIMutI CAGTG 2 cut(s) 476, 2166
BveI ACCTGC 2 cut(s) 2437, 2594
Cac8I GCNNGC 8 cut(s) 74, 483, 645, 1427, 1963, 2185, 2635, 2639
CciI TCATGA 1 cut(s) 2529
CfoI GCGC 3 cut(s) 89, 1075, 1105
Cfr10I RCCGGY 4 cut(s) 507, 525, 816, 2361
Cfr13I GGNCC 7 cut(s) 345, 513, 523, 630, 631, 650, 1203
Cfr9I CCCGGG 2 cut(s) 559, 959
ClaI ATCGAT 1 cut(s) 287
CpoI CGGWCCG 1 cut(s) 513
CseI GACGC 2 cut(s) 599, 1023
Csp6I GTAC 7 cut(s) 333, 576, 619, 680, 931, 2460, 2585
CspAI ACCGGT 1 cut(s) 525
CspCI CAANNNNNGTGG 2 cut(s) 682, 717
CspI CGGWCCG 1 cut(s) 513
CviQI GTAC 7 cut(s) 333, 576, 619, 680, 931, 2460, 2585
DdeI CTNAG 6 cut(s) 83, 936, 1297, 1328, 1525, 2312
DpnI GATC 6 cut(s) 290, 779, 834, 851, 1363, 1953
DpnII GATC 6 cut(s) 288, 777, 832, 849, 1361, 1951
DraI TTTAAA 1 cut(s) 1860
DrdI GACNNNNNNGTC 1 cut(s) 128
DriI GACNNNNNGTC 1 cut(s) 656
DseDI GACNNNNNNGTC 1 cut(s) 128
EaeI YGGCCR 3 cut(s) 1681, 2363, 2537
Eam1104I CTCTTC 5 cut(s) 50, 370, 1189, 1689, 1773
Eam1105I GACNNNNNGTC 1 cut(s) 656
EarI CTCTTC 5 cut(s) 50, 370, 1189, 1689, 1773
Eco130I CCWWGG 3 cut(s) 653, 1684, 2259
Eco147I AGGCCT 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 634
Eco31I GGTCTC 2 cut(s) 606, 865
Eco32I GATATC 1 cut(s) 744
Eco47I GGWCC 5 cut(s) 345, 513, 523, 650, 1203
Eco57I CTGAAG 1 cut(s) 148
Eco72I CACGTG 1 cut(s) 951
Eco88I CYCGRG 2 cut(s) 559, 959
EcoO109I RGGNCCY 2 cut(s) 630, 631
EcoRI GAATTC 1 cut(s) 999
EcoRII CCWGG 3 cut(s) 169, 179, 1344
EcoRV GATATC 1 cut(s) 744
EcoT14I CCWWGG 3 cut(s) 653, 1684, 2259
EcoT38I GRGCYC 1 cut(s) 634
ErhI CCWWGG 3 cut(s) 653, 1684, 2259
Esp3I CGTCTC 1 cut(s) 108
FalI AAGNNNNNCTT 2 cut(s) 1871, 1903
FaqI GGGAC 5 cut(s) 90, 536, 1216, 1241, 2071
FauI CCCGC 3 cut(s) 636, 1000, 1021
FauNDI CATATG 1 cut(s) 2074
FbaI TGATCA 1 cut(s) 832
FblI GTMKAC 2 cut(s) 548, 1097
FokI GGATG 3 cut(s) 14, 906, 1199
FriOI GRGCYC 1 cut(s) 634
GlaI GCGC 3 cut(s) 88, 1074, 1104
GsuI CTGGAG 2 cut(s) 297, 2484
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 6 cut(s) 306, 632, 1683, 1961, 2365, 2539
HgaI GACGC 2 cut(s) 599, 1023
HhaI GCGC 3 cut(s) 89, 1075, 1105
Hin6I GCGC 3 cut(s) 87, 1073, 1103
HinP1I GCGC 3 cut(s) 87, 1073, 1103
HincII GTYRAC 4 cut(s) 549, 727, 1098, 1470
HindII GTYRAC 4 cut(s) 549, 727, 1098, 1470
HindIII AAGCTT 1 cut(s) 1592
HphI GGTGA 4 cut(s) 308, 806, 2297, 2409
Hpy166II GTNNAC 9 cut(s) 549, 619, 680, 727, 942, 1038, 1098, 1111, 1470
Hpy188I TCNGA 8 cut(s) 113, 222, 865, 937, 1047, 1528, 2128, 2313
Hpy8I GTNNAC 9 cut(s) 549, 619, 680, 727, 942, 1038, 1098, 1111, 1470
Hpy99I CGWCG 4 cut(s) 20, 125, 1099, 1102
HpyAV CCTTC 9 cut(s) 59, 446, 1049, 1060, 1330, 1388, 2099, 2424, 2560
HpyCH4III ACNGT 6 cut(s) 104, 917, 1372, 2112, 2518, 2664
HpyCH4IV ACGT 4 cut(s) 123, 354, 950, 1873
HpyF10VI GCNNNNNNNGC 5 cut(s) 508, 1307, 1346, 1904, 2550
HpyF3I CTNAG 6 cut(s) 83, 936, 1297, 1328, 1525, 2312
HpySE526I ACGT 4 cut(s) 123, 354, 950, 1873
HspAI GCGC 3 cut(s) 87, 1073, 1103
Ksp22I TGATCA 1 cut(s) 832
Kzo9I GATC 6 cut(s) 288, 777, 832, 849, 1361, 1951
LmnI GCTCC 6 cut(s) 570, 672, 1031, 1619, 2035, 2486
LweI GCATC 3 cut(s) 193, 1276, 2392
MaeII ACGT 4 cut(s) 123, 354, 950, 1873
MaeIII GTNAC 6 cut(s) 355, 881, 917, 983, 1366, 2506
MalI GATC 6 cut(s) 290, 779, 834, 851, 1363, 1953
MbiI CCGCTC 1 cut(s) 675
MboI GATC 6 cut(s) 288, 777, 832, 849, 1361, 1951
MfeI CAATTG 2 cut(s) 1581, 2355
MflI RGATCY 2 cut(s) 1361, 1951
MhlI GDGCHC 2 cut(s) 634, 1855
MlsI TGGCCA 2 cut(s) 1683, 2539
MluNI TGGCCA 2 cut(s) 1683, 2539
MlyI GAGTC 8 cut(s) 699, 764, 884, 1085, 1322, 1817, 2462, 2585
MmeI TCCRAC 6 cut(s) 175, 888, 1025, 1756, 2047, 2203
Mox20I TGGCCA 2 cut(s) 1683, 2539
MroXI GAANNNNTTC 2 cut(s) 1713, 2663
MscI TGGCCA 2 cut(s) 1683, 2539
MseI TTAA 6 cut(s) 264, 1701, 1836, 1859, 1934, 2385
MslI CAYNNNNRTG 2 cut(s) 189, 1118
Msp20I TGGCCA 2 cut(s) 1683, 2539
MunI CAATTG 2 cut(s) 1581, 2355
Mva1269I GAATGC 2 cut(s) 1535, 2185
MvaI CCWGG 3 cut(s) 171, 181, 1346
MvnI CGCG 3 cut(s) 593, 1075, 1178
MwoI GCNNNNNNNGC 5 cut(s) 508, 1307, 1346, 1904, 2550
NciI CCSGG 9 cut(s) 517, 560, 561, 568, 960, 961, 1207, 1465, 2090
NdeI CATATG 1 cut(s) 2074
NdeII GATC 6 cut(s) 288, 777, 832, 849, 1361, 1951
NlaIV GGNNCC 8 cut(s) 524, 530, 565, 631, 632, 633, 1204, 1615
NmuCI GTSAC 4 cut(s) 881, 917, 983, 2506
NspI RCATGY 6 cut(s) 1044, 1109, 2231, 2392, 2637, 2680
NspV TTCGAA 1 cut(s) 1479
PaeI GCATGC 1 cut(s) 2637
PagI TCATGA 1 cut(s) 2529
PceI AGGCCT 1 cut(s) 306
PciI ACATGT 3 cut(s) 1040, 2227, 2388
PctI GAATGC 2 cut(s) 1535, 2185
PdmI GAANNNNTTC 2 cut(s) 1713, 2663
PfeI GAWTC 8 cut(s) 269, 800, 1484, 1753, 1786, 1925, 2273, 2288
PflMI CCANNNNNTGG 1 cut(s) 237
PinAI ACCGGT 1 cut(s) 525
PleI GAGTC 8 cut(s) 699, 764, 883, 1085, 1321, 1817, 2462, 2585
PmaCI CACGTG 1 cut(s) 951
PmlI CACGTG 1 cut(s) 951
PpsI GAGTC 8 cut(s) 699, 764, 883, 1085, 1321, 1817, 2462, 2585
Ppu21I YACGTR 1 cut(s) 951
PscI ACATGT 3 cut(s) 1040, 2227, 2388
PsiI TTATAA 1 cut(s) 1623
Psp1406I AACGTT 2 cut(s) 354, 1873
Psp6I CCWGG 3 cut(s) 169, 179, 1344
PspCI CACGTG 1 cut(s) 951
PspGI CCWGG 3 cut(s) 169, 179, 1344
PspN4I GGNNCC 8 cut(s) 524, 530, 565, 631, 632, 633, 1204, 1615
PspOMI GGGCCC 1 cut(s) 630
PspPI GGNCC 7 cut(s) 345, 513, 523, 630, 631, 650, 1203
PsrI GAACNNNNNNTAC 2 cut(s) 923, 955
PsuI RGATCY 2 cut(s) 1361, 1951
RsaI GTAC 7 cut(s) 334, 577, 620, 681, 932, 2461, 2586
RsaNI GTAC 7 cut(s) 333, 576, 619, 680, 931, 2460, 2585
RseI CAYNNNNRTG 2 cut(s) 189, 1118
Rsr2I CGGWCCG 1 cut(s) 513
RsrII CGGWCCG 1 cut(s) 513
SalI GTCGAC 2 cut(s) 547, 1096
SaqAI TTAA 6 cut(s) 264, 1701, 1836, 1859, 1934, 2385
Sau3AI GATC 6 cut(s) 288, 777, 832, 849, 1361, 1951
Sau96I GGNCC 7 cut(s) 345, 513, 523, 630, 631, 650, 1203
ScaI AGTACT 2 cut(s) 932, 2461
SchI GAGTC 8 cut(s) 699, 764, 884, 1085, 1322, 1817, 2462, 2585
SduI GDGCHC 2 cut(s) 634, 1855
SfaNI GCATC 3 cut(s) 193, 1276, 2392
SfuI TTCGAA 1 cut(s) 1479
SgrDI CGTCGACG 1 cut(s) 1096
SinI GGWCC 5 cut(s) 345, 513, 523, 650, 1203
SmaI CCCGGG 2 cut(s) 561, 961
SmiMI CAYNNNNRTG 2 cut(s) 189, 1118
SmlI CTYRAG 2 cut(s) 419, 2654
SmoI CTYRAG 2 cut(s) 419, 2654
SpeI ACTAGT 1 cut(s) 2456
SphI GCATGC 1 cut(s) 2637
SseBI AGGCCT 1 cut(s) 306
SspI AATATT 1 cut(s) 2025
StuI AGGCCT 1 cut(s) 306
StyI CCWWGG 3 cut(s) 653, 1684, 2259
TaaI ACNGT 6 cut(s) 104, 917, 1372, 2112, 2518, 2664
TaiI ACGT 4 cut(s) 126, 357, 953, 1876
TaqI TCGA 8 cut(s) 18, 287, 548, 852, 1097, 1479, 1928, 2682
TaqII GACCGA 1 cut(s) 342
TatI WGTACW 4 cut(s) 575, 618, 930, 2459
TfiI GAWTC 8 cut(s) 269, 800, 1484, 1753, 1786, 1925, 2273, 2288
Tru1I TTAA 6 cut(s) 264, 1701, 1836, 1859, 1934, 2385
Tru9I TTAA 6 cut(s) 264, 1701, 1836, 1859, 1934, 2385
TscAI CASTG 2 cut(s) 483, 2173
TseFI GTSAC 4 cut(s) 881, 917, 983, 2506
Tsp45I GTSAC 4 cut(s) 881, 917, 983, 2506
TspGWI ACGGA 3 cut(s) 333, 1065, 1606
TspMI CCCGGG 2 cut(s) 559, 959
TspRI CASTG 2 cut(s) 483, 2173
Van91I CCANNNNNTGG 1 cut(s) 237
VpaK11BI GGWCC 5 cut(s) 345, 513, 523, 650, 1203
XapI RAATTY 4 cut(s) 175, 999, 1442, 2215
XceI RCATGY 6 cut(s) 1044, 1109, 2231, 2392, 2637, 2680
XmaI CCCGGG 2 cut(s) 559, 959
XmiI GTMKAC 2 cut(s) 548, 1097
XmnI GAANNNNTTC 2 cut(s) 1713, 2663
ZrmI AGTACT 2 cut(s) 932, 2461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.