Rh5BG071900

toxin transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010603
Physical Location & Seq
Reverse (-)
5858211 .. 5881005
22795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG071900.1

Sequence Viewer

Length: 738 bp
ATGGGGGTTAAAGTAGATCATTATAAGGTTCTAGATTTACCCTCTGGTGAGGAAGGTGCTAAGCTCACACACAGAGAGATTTTGAAGGCATACAGAGCAAAGGCGTTGCTGTTGCATCCAGACAAGAGACCAGACTATCCCGGCGCCAACGCCGAGTTTCAACAGCTCAGTTCATCCTATGAGATTCTCAAGGACCCGAAATCCCGGGCAGAGTTCGACGCTGCTCTAGAGCTCGACAACACTCCAGAGCTCGAGAAGGAGTACGATGAATTGAGTCTTGTTACTATGTTGATTCTGGTTGCTGCTCTTTCCATTTTGTATGCAACAAACCTCCTTCAACATAATCACCGAACCCTTCAGGGGAAACCCTCGTTTGCAGTCAATCGTTTCATTTCTCAGATGTCCAAGCCGTTGATTGCTGAGATTTATGGTTTTTGCTCTGCTGGGTTTTGTTATCTGCTTCAATTTTTGTCAAAGAAAATGTCTTTTTTGTTGAAAAGCGATTCGGAATATCGATTGGAAGCGCAAGGAACAGATCGAGTTGAAGCGCCGTTATGGCAATTACAAGCCCCTAAAGAAACCGAGGAAACCACAGCTGCAGTGTTGGATTTTCTGATGGATGCAAATGTTGGGCTAAGTTTACTGCCCTTAATGGTGATGTATAACAGCTCAACCTGTAAGTCAAAACCAAGTGGTTGGAAATCAAGTGGCGGTGACCCTTGTGGTGATTCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01100 ko01212 ko03010 map00062 map01100 map01212 map03010
Pfam Domains
Protein Families
DnaJ DnaJ-X

Protein Analysis

245

Amino Acids

27.29

Weight (kDa)

5.45

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 6 - 73 2.2e-16 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 24
AccB1I GGYRCC 1 cut(s) 143
AciI CCGC 1 cut(s) 711
AcuI CTGAAG 1 cut(s) 341
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 1 cut(s) 360
AgsI TTSAA 7 cut(s) 85, 161, 338, 464, 496, 545, 732
AluBI AGCT 6 cut(s) 64, 166, 232, 250, 596, 669
AluI AGCT 6 cut(s) 64, 166, 232, 250, 596, 669
Alw21I GWGCWC 2 cut(s) 234, 252
Alw26I GTCTC 1 cut(s) 121
Ama87I CYCGRG 2 cut(s) 204, 251
ApeKI GCWGC 3 cut(s) 221, 302, 596
ArsI GACNNNNNNTTYG 2 cut(s) 467, 499
AspLEI GCGC 3 cut(s) 146, 526, 550
AspS9I GGNCC 1 cut(s) 193
AsuC2I CCSGG 3 cut(s) 141, 205, 206
AsuHPI GGTGA 5 cut(s) 59, 338, 667, 725, 737
AvaI CYCGRG 2 cut(s) 204, 251
AvaII GGWCC 1 cut(s) 193
BanI GGYRCC 1 cut(s) 143
BanII GRGCYC 2 cut(s) 234, 252
Bbv12I GWGCWC 2 cut(s) 234, 252
BbvI GCAGC 3 cut(s) 208, 289, 583
BccI CCATC 1 cut(s) 610
BceAI ACGGC 2 cut(s) 394, 535
BcnI CCSGG 3 cut(s) 141, 205, 206
BcoDI GTCTC 1 cut(s) 121
BfaI CTAG 2 cut(s) 32, 227
BfmI CTRYAG 1 cut(s) 597
BfoI RGCGCY 2 cut(s) 147, 551
BglI GCCNNNNNGGC 1 cut(s) 556
BisI GCNGC 3 cut(s) 222, 303, 597
BlpI GCTNAGC 1 cut(s) 60
BlsI GCNGC 3 cut(s) 223, 304, 598
Bme1390I CCNGG 3 cut(s) 141, 205, 206
Bme18I GGWCC 1 cut(s) 193
BmeT110I CYCGRG 2 cut(s) 204, 251
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 2 cut(s) 145, 195
BmrFI CCNGG 3 cut(s) 141, 205, 206
BmsI GCATC 2 cut(s) 124, 610
BpmI CTGGAG 1 cut(s) 228
Bpu1102I GCTNAGC 1 cut(s) 60
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 3 cut(s) 141, 205, 206
Bsa29I ATCGAT 1 cut(s) 514
BsaHI GRCGYC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 121
BsaJI CCNNGG 2 cut(s) 204, 582
Bsc4I CCNNNNNNNGG 1 cut(s) 360
BseCI ATCGAT 1 cut(s) 514
BseDI CCNNGG 2 cut(s) 204, 582
BseGI GGATG 3 cut(s) 115, 173, 625
BseLI CCNNNNNNNGG 1 cut(s) 360
BseMII CTCAG 3 cut(s) 181, 410, 411
BseXI GCAGC 3 cut(s) 208, 289, 583
BseYI CCCAGC 1 cut(s) 443
BshNI GGYRCC 1 cut(s) 143
BshVI ATCGAT 1 cut(s) 514
BsiHKAI GWGCWC 2 cut(s) 234, 252
BsiHKCI CYCGRG 2 cut(s) 204, 251
BsiSI CCGG 2 cut(s) 141, 205
BslI CCNNNNNNNGG 1 cut(s) 360
BsmAI GTCTC 1 cut(s) 121
Bso31I GGTCTC 1 cut(s) 121
BsoBI CYCGRG 2 cut(s) 204, 251
Bsp1286I GDGCHC 2 cut(s) 234, 252
Bsp143I GATC 2 cut(s) 16, 535
Bsp1720I GCTNAGC 1 cut(s) 60
BspACI CCGC 1 cut(s) 711
BspCNI CTCAG 3 cut(s) 180, 409, 412
BspDI ATCGAT 1 cut(s) 514
BspLI GGNNCC 2 cut(s) 145, 195
BspMAI CTGCAG 1 cut(s) 601
BspT107I GGYRCC 1 cut(s) 143
BspTNI GGTCTC 1 cut(s) 121
BssECI CCNNGG 2 cut(s) 204, 582
BssMI GATC 2 cut(s) 16, 535
BssNI GRCGYC 1 cut(s) 144
BstACI GRCGYC 1 cut(s) 144
BstDEI CTNAG 5 cut(s) 60, 167, 396, 420, 635
BstEII GGTNACC 1 cut(s) 713
BstF5I GGATG 3 cut(s) 115, 173, 625
BstH2I RGCGCY 2 cut(s) 147, 551
BstHHI GCGC 3 cut(s) 146, 526, 550
BstKTI GATC 2 cut(s) 19, 538
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 2 cut(s) 16, 535
BstMWI GCNNNNNNNGC 2 cut(s) 95, 556
BstPI GGTNACC 1 cut(s) 713
BstSCI CCNGG 3 cut(s) 139, 203, 204
BstSFI CTRYAG 1 cut(s) 597
BstV1I GCAGC 3 cut(s) 208, 289, 583
BstXI CCANNNNNNTGG 1 cut(s) 696
Bsu15I ATCGAT 1 cut(s) 514
BsuTUI ATCGAT 1 cut(s) 514
BtsCI GGATG 3 cut(s) 115, 173, 625
BtsI GCAGTG 1 cut(s) 606
BtsIMutI CAGTG 1 cut(s) 606
CfoI GCGC 3 cut(s) 146, 526, 550
Cfr13I GGNCC 1 cut(s) 193
Cfr9I CCCGGG 1 cut(s) 204
ClaI ATCGAT 1 cut(s) 514
CseI GACGC 1 cut(s) 227
Csp6I GTAC 1 cut(s) 262
CviJI RGCY 9 cut(s) 64, 166, 232, 250, 409, 569, 596, 634, 669
CviKI_1 RGCY 9 cut(s) 64, 166, 232, 250, 409, 569, 596, 634, 669
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 5 cut(s) 60, 167, 396, 420, 635
DinI GGCGCC 1 cut(s) 145
DpnI GATC 2 cut(s) 18, 537
DpnII GATC 2 cut(s) 16, 535
Ecl136II GAGCTC 2 cut(s) 232, 250
Eco24I GRGCYC 2 cut(s) 234, 252
Eco31I GGTCTC 1 cut(s) 121
Eco47I GGWCC 1 cut(s) 193
Eco53kI GAGCTC 2 cut(s) 232, 250
Eco57I CTGAAG 1 cut(s) 341
Eco88I CYCGRG 2 cut(s) 204, 251
Eco91I GGTNACC 1 cut(s) 713
EcoICRI GAGCTC 2 cut(s) 232, 250
EcoO109I RGGNCCY 1 cut(s) 193
EcoO65I GGTNACC 1 cut(s) 713
EcoT38I GRGCYC 2 cut(s) 234, 252
EgeI GGCGCC 1 cut(s) 145
EheI GGCGCC 1 cut(s) 145
FaiI YATR 9 cut(s) 24, 91, 180, 287, 321, 342, 429, 556, 663
Fnu4HI GCNGC 3 cut(s) 222, 303, 597
FokI GGATG 3 cut(s) 102, 160, 632
FriOI GRGCYC 2 cut(s) 234, 252
Fsp4HI GCNGC 3 cut(s) 222, 303, 597
FspBI CTAG 2 cut(s) 32, 227
GlaI GCGC 3 cut(s) 145, 525, 549
GluI GCNGC 3 cut(s) 222, 303, 597
GsaI CCCAGC 1 cut(s) 447
GsuI CTGGAG 1 cut(s) 228
HaeII RGCGCY 2 cut(s) 147, 551
HapII CCGG 2 cut(s) 141, 205
HgaI GACGC 1 cut(s) 227
HhaI GCGC 3 cut(s) 146, 526, 550
Hin1I GRCGYC 1 cut(s) 144
Hin6I GCGC 3 cut(s) 144, 524, 548
HinP1I GCGC 3 cut(s) 144, 524, 548
HinfI GANTC 5 cut(s) 184, 274, 292, 503, 728
HpaII CCGG 2 cut(s) 141, 205
HphI GGTGA 5 cut(s) 59, 338, 667, 725, 737
Hpy166II GTNNAC 1 cut(s) 641
Hpy188I TCNGA 3 cut(s) 399, 508, 615
Hpy188III TCNNGA 5 cut(s) 32, 119, 227, 245, 253
Hpy8I GTNNAC 1 cut(s) 641
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 5 cut(s) 47, 79, 250, 344, 365
HpyCH4V TGCA 5 cut(s) 115, 323, 377, 599, 623
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 556
HpyF3I CTNAG 5 cut(s) 60, 167, 396, 420, 635
Hsp92I GRCGYC 1 cut(s) 144
HspAI GCGC 3 cut(s) 144, 524, 548
KasI GGCGCC 1 cut(s) 143
Kzo9I GATC 2 cut(s) 16, 535
Lsp1109I GCAGC 3 cut(s) 208, 289, 583
LweI GCATC 2 cut(s) 124, 610
MaeI CTAG 2 cut(s) 32, 227
MaeIII GTNAC 2 cut(s) 280, 713
MalI GATC 2 cut(s) 18, 537
MboI GATC 2 cut(s) 16, 535
MhlI GDGCHC 2 cut(s) 234, 252
MluCI AATT 4 cut(s) 269, 464, 560, 733
Mly113I GGCGCC 1 cut(s) 144
MlyI GAGTC 1 cut(s) 283
MmeI TCCRAC 2 cut(s) 585, 677
MnlI CCTC 5 cut(s) 43, 52, 341, 379, 577
MseI TTAA 2 cut(s) 9, 650
MspA1I CMGCKG 1 cut(s) 596
MspI CCGG 2 cut(s) 141, 205
MspR9I CCNGG 3 cut(s) 141, 205, 206
MwoI GCNNNNNNNGC 2 cut(s) 95, 556
NarI GGCGCC 1 cut(s) 144
NciI CCSGG 3 cut(s) 141, 205, 206
NdeII GATC 2 cut(s) 16, 535
NlaIV GGNNCC 2 cut(s) 145, 195
NmeAIII GCCGAG 1 cut(s) 178
NmuCI GTSAC 1 cut(s) 713
PaeR7I CTCGAG 1 cut(s) 251
PfeI GAWTC 4 cut(s) 184, 292, 503, 728
PkrI GCNGC 3 cut(s) 223, 304, 598
PleI GAGTC 1 cut(s) 282
PluTI GGCGCC 1 cut(s) 147
PpsI GAGTC 1 cut(s) 282
PpuMI RGGWCCY 1 cut(s) 193
PsiI TTATAA 1 cut(s) 24
Psp124BI GAGCTC 2 cut(s) 234, 252
Psp5II RGGWCCY 1 cut(s) 193
PspEI GGTNACC 1 cut(s) 713
PspFI CCCAGC 1 cut(s) 443
PspN4I GGNNCC 2 cut(s) 145, 195
PspPI GGNCC 1 cut(s) 193
PspPPI RGGWCCY 1 cut(s) 193
PstI CTGCAG 1 cut(s) 601
PvuII CAGCTG 1 cut(s) 596
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
SacI GAGCTC 2 cut(s) 234, 252
SaqAI TTAA 2 cut(s) 9, 650
SatI GCNGC 3 cut(s) 222, 303, 597
Sau3AI GATC 2 cut(s) 16, 535
Sau96I GGNCC 1 cut(s) 193
SchI GAGTC 1 cut(s) 283
ScrFI CCNGG 3 cut(s) 141, 205, 206
SduI GDGCHC 2 cut(s) 234, 252
SfaNI GCATC 2 cut(s) 124, 610
SfcI CTRYAG 1 cut(s) 597
SfoI GGCGCC 1 cut(s) 145
Sfr274I CTCGAG 1 cut(s) 251
SinI GGWCC 1 cut(s) 193
SlaI CTCGAG 1 cut(s) 251
SmaI CCCGGG 1 cut(s) 206
SmlI CTYRAG 2 cut(s) 188, 251
SmoI CTYRAG 2 cut(s) 188, 251
Sse9I AATT 4 cut(s) 269, 464, 560, 733
SsiI CCGC 1 cut(s) 711
SspDI GGCGCC 1 cut(s) 143
SspMI CTAG 2 cut(s) 32, 227
SstI GAGCTC 2 cut(s) 234, 252
StyD4I CCNGG 3 cut(s) 139, 203, 204
TaqI TCGA 5 cut(s) 216, 234, 252, 514, 538
TasI AATT 4 cut(s) 269, 464, 560, 733
TfiI GAWTC 4 cut(s) 184, 292, 503, 728
Tru1I TTAA 2 cut(s) 9, 650
Tru9I TTAA 2 cut(s) 9, 650
TscAI CASTG 1 cut(s) 606
TseFI GTSAC 1 cut(s) 713
TseI GCWGC 3 cut(s) 221, 302, 596
Tsp45I GTSAC 1 cut(s) 713
TspDTI ATGAA 3 cut(s) 162, 282, 379
TspMI CCCGGG 1 cut(s) 204
TspRI CASTG 1 cut(s) 606
VpaK11BI GGWCC 1 cut(s) 193
XbaI TCTAGA 2 cut(s) 31, 226
XhoI CTCGAG 1 cut(s) 251
XmaI CCCGGG 1 cut(s) 204
XspI CTAG 2 cut(s) 32, 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.