Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0002753

Physical Location & Seq

Genomic Coordinates Reverse (-)

10847648 .. 10856588

Gene Structure 8941 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00088970.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2496 bp

ATGTTGGGTCTATGGCGTTTTGCTAAAGGTTTGGCAGTCAAAGCAGTGGCTGATGATTTATTTCTGTTTCGATTTTTGCTTCATGGTGATTTGCAGAAGGTGCTGGACAGAGAGCCATGGTCTTTTGCTGGTAACCTTCTTATGTTGATACCGGTGGACTCTTCTTCCAACATTCATAATCTCTCATTCACTCATGTTTCTCTTTGGGTACATGCTCATAACATTTCTTGGTTGAGAATGTCTGATGATCTAGCAAAGTTAATTGGGGACTCCCTGGGTTTATTGATGTTGGACGGGATGGAGACTTTGGGTGTATTGGCAAAACTCTCCGCATCCGAGTTCGCTGGGATGTCTCTCAGCCGTTGTGTCGTGAAACAACCATTGACTTGGATGAAGTTGGTGACAGAGAAGTCAGCTTCATGGAAGTTGAGGGAGACTGAAAATGTCCCAACTGTAAAGAGATTTCCTCGATCATCACCGGCAAATTCCATCAGTCGACTAGCATCTCTGTCGGGATCTGATGTTAGACGCAGCAGGCACTCACCTGAGCAGTTGGGACTGGAACCTGGCAGTTATCAAGCAGTTACGCACAAGAAGCAGGAGGAGGAGGAGATTCTCATATGTGAATGCAAGTATGATGAACAGGATCTTGAAAGTGCATGTGGAGAAAGGTGCTTGAATGAGTTGACCAGCACGCAATGCACTCCAGGATATCGCCCTTGTGGTGTCTATTGTAAGAATCAGAATTCAGACATGGTTAAGGAAGAAAGGCCACAAATTACAAGGAGAGCAACCAGATCCTCTGCTTTGGCTAAAAGTGTAGTTGAAGAAACAGACACAAGCAGTCAGCACCCAAATGATGCTTCTGCGAGTTCAGACATTATTAAAGAAGAAAGCCCACAAATTACAAGGAGAACAACCCGATCCTCCGTTTTGGCCAAATGTTCAAACAATGTAGTTGAAGAAACAGATGCAAATAGTCAGCAGCAACCGACTTTAGATGACCTTGTGATTGGAGAAGGAGAGCCCTTTAGCTTCGATGATCTGGTGTCTGGTTTTCCTGGTAGAGAAAGTCAGATCAAAGAGCTTGTGCACCTTTTGGGTCCGCAGAACTCTCCCATGCTTCCTATATTTATATATGGAGGCTCTTCCACTGGAAAAACCAGTATTGTTCTACAAACATTTAGGCATCTTAAACGACCTTTTGTTTACTCAAGCTGCCTTACCTGTTATAATCCTCGAATCTTGTTTGAATCCATATTAAATCAATTGTTCCTTCAACGAAAGAAAGCAGAAAATGGTTATTCTAGTGCAAAGCGCTGTGAAAGGCCATCTGATTTTGTGAATTTTCTTCGCAAAGCCTTGGTTAGCATTATTGGTAATCCTCAGGGACAAAAGTCAGGGTTGAGTAGCAAAAGGTCGGGAAGGGCTAATGGAAATATGATCTACTTGATTTTTGACAATTTGGAGCGTGTCAAAGGGTGGGACAAAGGTTCTACTGTGCTACCTTTGTTGTTTAATCTCTATGACACTCTGAAAATGCCCGAGGTCGGCCTTATCTTCGTCAGCAATACCTCACCGGATACATATGACTCAAATATGGGTTATGTTGAGCCTAACCCTCTATATCTTCCTGATTATACAGATGATGATCTTGGTCAAATCTTAATGAGACACCAAGCAAACAAAAAGCTTTATACCTCTTTTCTCAGTGTTGTACTAAGGCCTTTCAGTAGAATTACCAGACGAGTGGATGAATTGTCTAGTGCATTTTCACTATTATTCAGAAAATATTGTGAACCTATTACTGATCTTGGGGTTGTTCCCAATGAAGAAATGAAGAGAAAGTTGTTTAGTCAATTTCAGCCCCATATTGCTCCTACACTGAATGAGATATTTAAGGTTTCAACTCAGCCTTCCACTGAAGTTGAAGCTAGGGAGAGAAAGCCAAAGGTTATCACAAGGAAATCCGGAGGTTATGAAGTTGTTGACCAGTTAGATTTTCACATGTCTACCTCTGCCAAGTATCTTCTAATTTCAGCATTCCTTGCTTCAAGAAATCCAGCTACCCTTGATGCATCATTGTTCGATTCTACTGGAGGTTCTAATAATCGAAAACGAAAGAGGAAGGTCTCTGAGAAGTCAATGGAACAGAAGGAAACTGCAGAACAGGAATTACTCATGAAGGGCCCTGGAACATTCCCAATGGAGAGGTTACTAGCCATATTCCAGTGTATTACATCTGTGGCGGAAGATCCACTTGATGATGAGGAACAAGCGCAGGATGGGTTAGGGATTCAAGATGGGCATTGTGGGCTCATGTCTGATGTCCTCTTACAATTGAGTAGTCTCTGTGGTGCAAATTTTATTGTCAAAGGAGGAAGCTGCCCACTCGAGGGCTCAACTCGCTATCGTTCTACAGTTAGTGAAGATATGGCCCTGCAGGTAGCAAGGAGCATTAAGTTTCCTCTCTCAAAGTACTTGTACAGAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000808 GO:0003674 GO:0003676 GO:0003677 GO:0003688 GO:0003690 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005664 GO:0005694 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009743 GO:0009744 GO:0009987 GO:0010033 GO:0031974 GO:0031981 GO:0032991 GO:0034285 GO:0034641 GO:0034645 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0050896 GO:0070013 GO:0071704 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901576 GO:1901700 GO:1990837

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 map04110 map04111 map04113

Pfam Domains

Protein Families

AAA_16 ORC5_C

Protein Analysis

831

Amino Acids

93.03

Weight (kDa)

7.78

Isoelectric Point (pI)

53.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF4283	PF14111	3 - 51	6.2e-06	Domain of unknown function (DUF4283)
AWS	PF17907	216 - 248	1e-06	AWS domain
AAA_16	PF13191	353 - 512	7.9e-12	AAA ATPase domain
ORC5_lid	PF21639	566 - 619	2.2e-11	ORC5, lid domain
ORC5_C	PF14630	670 - 828	4.4e-39	Origin recognition complex (ORC) subunit 5 C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002753)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29910 AT4G29910
fragaria_vesca	FvH4_7g14490 FvH4_7g14490 FvH4_7g14490 FvH4_7g14490 FvH4_7g14490
malus_domestica	MD01G1065000.v1.1 MD07G1135400.v1.1
prunus_persica	Prupe.2G172000_v2.0.a1 Prupe.2G172000_v2.0.a1 Prupe.2G172000_v2.0.a1
pyrus_communis	pycom01g09430 pycom07g13260
rosa_chinensis	RchiOBHm_Chr1g0355901 RchiOBHm_Chr2g0161261
rosa_laevigata	RLG00000021273 RLG00000028117
rosa_multiflora	Rmu_sc0004705.1_g000017 Rmu_sc0009869.1_g000009
rosa_roxburghii	Rroxscaffold_2G00088970 Rroxscaffold_2G00110130 Rroxscaffold_4G00299930
rosa_rugosa	Rorug01G0248300 Rorug01G0248400 Rorug01G0248500 Rorug02G0487300 Rorug02G0487400 Rorug02G0487500
rosa_samantha	Rh1AG261600 Rh1BG229700 Rh1CG244700 Rh1DG257700 Rh2AG554200 Rh2BG567600 Rh2CG538100 Rh2DG577000
rosa_wichuraiana	Rw1G023100 Rw2G045850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1233
AasI	GACNNNNNNGTC	1 cut(s)	409
Acc36I	ACCTGC	1 cut(s)	2434
AccI	GTMKAC	2 cut(s)	496, 2012
AccIII	TCCGGA	1 cut(s)	1970
AciI	CCGC	3 cut(s)	330, 1106, 2249
AclWI	GGATC	5 cut(s)	523, 654, 792, 918, 2249
AcoI	YGGCCR	1 cut(s)	936
AcsI	RAATTY	4 cut(s)	484, 745, 1345, 2362
AcuI	CTGAAG	1 cut(s)	1944
AfaI	GTAC	4 cut(s)	210, 1719, 2480, 2486
AfeI	AGCGCT	1 cut(s)	1319
AfiI	CCNNNNNNNGG	3 cut(s)	1550, 2395, 2396
AflIII	ACRYGT	1 cut(s)	2007
AgeI	ACCGGT	1 cut(s)	151
AjnI	CCWGG	5 cut(s)	273, 565, 706, 1060, 2191
AjuI	GAANNNNNNNTTGG	2 cut(s)	847, 879
AluBI	AGCT	8 cut(s)	416, 1035, 1087, 1218, 1693, 1934, 2066, 2385
AluI	AGCT	8 cut(s)	416, 1035, 1087, 1218, 1693, 1934, 2066, 2385
Alw21I	GWGCWC	1 cut(s)	1095
Alw26I	GTCTC	6 cut(s)	296, 357, 428, 1666, 2137, 2354
Alw44I	GTGCAC	1 cut(s)	1091
AlwI	GGATC	5 cut(s)	523, 654, 792, 918, 2249
AlwNI	CAGNNNCTG	1 cut(s)	50
Ama87I	CYCGRG	2 cut(s)	1544, 2393
Aor13HI	TCCGGA	1 cut(s)	1970
Aor51HI	AGCGCT	1 cut(s)	1319
AoxI	GGCC	7 cut(s)	770, 936, 1328, 1552, 1724, 2188, 2436
ApaI	GGGCCC	1 cut(s)	2192
ApaLI	GTGCAC	1 cut(s)	1091
ApeKI	GCWGC	4 cut(s)	531, 985, 1218, 2385
ApoI	RAATTY	4 cut(s)	484, 745, 1345, 2362
AsiGI	ACCGGT	1 cut(s)	151
AspLEI	GCGC	2 cut(s)	1320, 2281
AspS9I	GGNCC	4 cut(s)	1103, 2188, 2189, 2437
AsuHPI	GGTGA	5 cut(s)	98, 412, 468, 534, 1569
AvaI	CYCGRG	2 cut(s)	1544, 2393
AvaII	GGWCC	1 cut(s)	1103
AxyI	CCTNAGG	1 cut(s)	1386
BaeGI	GKGCMC	2 cut(s)	1095, 2192
BalI	TGGCCA	1 cut(s)	938
BanII	GRGCYC	4 cut(s)	1029, 2192, 2319, 2402
Bbv12I	GWGCWC	1 cut(s)	1095
BbvI	GCAGC	4 cut(s)	543, 997, 1205, 2372
BccI	CCATC	5 cut(s)	292, 497, 1339, 2279, 2297
BceAI	ACGGC	1 cut(s)	345
BcgI	CGANNNNNNTGC	2 cut(s)	492, 526
BciT130I	CCWGG	5 cut(s)	275, 567, 708, 1062, 2193
BciVI	GTATCC	1 cut(s)	1576
BcoDI	GTCTC	6 cut(s)	296, 357, 428, 1666, 2137, 2354
BfaI	CTAG	6 cut(s)	251, 500, 1308, 1764, 1935, 2219
BfmI	CTRYAG	3 cut(s)	2163, 2418, 2441
BfoI	RGCGCY	1 cut(s)	1321
BfuAI	ACCTGC	1 cut(s)	2434
BfuI	GTATCC	1 cut(s)	1576
BisI	GCNGC	4 cut(s)	532, 986, 1219, 2386
BlsI	GCNGC	4 cut(s)	533, 987, 1220, 2387
BmcAI	AGTACT	1 cut(s)	2480
Bme1390I	CCNGG	5 cut(s)	275, 567, 708, 1062, 2193
Bme18I	GGWCC	1 cut(s)	1103
BmeT110I	CYCGRG	2 cut(s)	1544, 2393
BmgT120I	GGNCC	4 cut(s)	1103, 2188, 2189, 2437
BmiI	GGNNCC	3 cut(s)	564, 1104, 2190
BmrFI	CCNGG	5 cut(s)	275, 567, 708, 1062, 2193
BmsI	GCATC	7 cut(s)	341, 512, 850, 961, 1198, 2065, 2087
BoxI	GACNNNNGTC	1 cut(s)	1396
BplI	GAGNNNNNCTC	2 cut(s)	451, 483
BpmI	CTGGAG	2 cut(s)	690, 2118
Bpu10I	CCTNAGC	1 cut(s)	546
BpuEI	CTTGAG	1 cut(s)	1198
BsaBI	GATNNNNATC	1 cut(s)	1650
BsaI	GGTCTC	1 cut(s)	2137
BsaJI	CCNNGG	6 cut(s)	116, 273, 274, 1362, 1545, 2191
BsaWI	WCCGGW	3 cut(s)	151, 1579, 1970
Bsc4I	CCNNNNNNNGG	3 cut(s)	1550, 2395, 2396
Bse118I	RCCGGY	2 cut(s)	151, 478
Bse1I	ACTGG	6 cut(s)	564, 1159, 1164, 1993, 2101, 2230
Bse21I	CCTNAGG	1 cut(s)	1386
Bse3DI	GCAATG	1 cut(s)	704
Bse8I	GATNNNNATC	1 cut(s)	1650
BseAI	TCCGGA	1 cut(s)	1970
BseBI	CCWGG	5 cut(s)	275, 567, 708, 1062, 2193
BseDI	CCNNGG	6 cut(s)	116, 273, 274, 1362, 1545, 2191
BseGI	GGATG	6 cut(s)	303, 332, 354, 396, 1759, 2290
BseJI	GATNNNNATC	1 cut(s)	1650
BseLI	CCNNNNNNNGG	3 cut(s)	1550, 2395, 2396
BseMI	GCAATG	1 cut(s)	704
BseMII	CTCAG	6 cut(s)	370, 537, 1400, 1723, 1925, 2127
BseNI	ACTGG	6 cut(s)	564, 1159, 1164, 1993, 2101, 2230
BseRI	GAGGAG	3 cut(s)	617, 620, 623
BseSI	GKGCMC	2 cut(s)	1095, 2192
BseXI	GCAGC	4 cut(s)	543, 997, 1205, 2372
BseYI	CCCAGC	1 cut(s)	344
BshFI	GGCC	7 cut(s)	772, 938, 1330, 1554, 1726, 2190, 2438
BshTI	ACCGGT	1 cut(s)	151
BsiHKAI	GWGCWC	1 cut(s)	1095
BsiHKCI	CYCGRG	2 cut(s)	1544, 2393
BsiSI	CCGG	4 cut(s)	152, 479, 1580, 1971
BslFI	GGGAC	5 cut(s)	281, 431, 570, 1404, 1499
BslI	CCNNNNNNNGG	3 cut(s)	1550, 2395, 2396
BsmAI	GTCTC	6 cut(s)	296, 357, 428, 1666, 2137, 2354
BsmFI	GGGAC	5 cut(s)	281, 431, 570, 1404, 1499
BsmI	GAATGC	2 cut(s)	632, 2042
BsnI	GGCC	7 cut(s)	772, 938, 1330, 1554, 1726, 2190, 2438
Bso31I	GGTCTC	1 cut(s)	2137
BsoBI	CYCGRG	2 cut(s)	1544, 2393
Bsp120I	GGGCCC	1 cut(s)	2188
Bsp1286I	GDGCHC	5 cut(s)	1029, 1095, 2192, 2319, 2402
Bsp13I	TCCGGA	1 cut(s)	1970
Bsp1407I	TGTACA	1 cut(s)	2484
Bsp19I	CCATGG	1 cut(s)	116
BspACI	CCGC	3 cut(s)	330, 1106, 2249
BspANI	GGCC	7 cut(s)	772, 938, 1330, 1554, 1726, 2190, 2438
BspCNI	CTCAG	6 cut(s)	369, 538, 1399, 1722, 1924, 2128
BspEI	TCCGGA	1 cut(s)	1970
BspHI	TCATGA	1 cut(s)	2181
BspLI	GGNNCC	3 cut(s)	564, 1104, 2190
BspMAI	CTGCAG	2 cut(s)	2167, 2445
BspMI	ACCTGC	1 cut(s)	2434
BspPI	GGATC	5 cut(s)	523, 654, 792, 918, 2249
BspQI	GCTCTTC	1 cut(s)	1153
BspTNI	GGTCTC	1 cut(s)	2137
BsrDI	GCAATG	1 cut(s)	704
BsrFI	RCCGGY	2 cut(s)	151, 478
BsrGI	TGTACA	1 cut(s)	2484
BsrI	ACTGG	6 cut(s)	564, 1159, 1164, 1993, 2101, 2230
BssAI	RCCGGY	2 cut(s)	151, 478
BssECI	CCNNGG	6 cut(s)	116, 273, 274, 1362, 1545, 2191
BssT1I	CCWWGG	2 cut(s)	116, 1362
Bst2UI	CCWGG	5 cut(s)	275, 567, 708, 1062, 2193
Bst4CI	ACNGT	3 cut(s)	454, 1501, 2422
Bst6I	CTCTTC	3 cut(s)	166, 1153, 1835
BstAPI	GCANNNNNTGC	3 cut(s)	100, 699, 2048
BstAUI	TGTACA	1 cut(s)	2484
BstC8I	GCNNGC	2 cut(s)	536, 695
BstDEI	CTNAG	7 cut(s)	356, 546, 1386, 1709, 1721, 1911, 2136
BstDSI	CCRYGG	1 cut(s)	116
BstEII	GGTNACC	1 cut(s)	131
BstF5I	GGATG	6 cut(s)	303, 332, 354, 396, 1759, 2290
BstH2I	RGCGCY	1 cut(s)	1321
BstHHI	GCGC	2 cut(s)	1320, 2281
BstMAI	GTCTC	6 cut(s)	296, 357, 428, 1666, 2137, 2354
BstMWI	GCNNNNNNNGC	7 cut(s)	41, 100, 595, 699, 2048, 2314, 2406
BstNI	CCWGG	5 cut(s)	275, 567, 708, 1062, 2193
BstNSI	RCATGY	3 cut(s)	215, 663, 2011
BstPAI	GACNNNNGTC	1 cut(s)	1396
BstPI	GGTNACC	1 cut(s)	131
BstSCI	CCNGG	5 cut(s)	273, 565, 706, 1060, 2191
BstSFI	CTRYAG	3 cut(s)	2163, 2418, 2441
BstSLI	GKGCMC	2 cut(s)	1095, 2192
BstV1I	GCAGC	4 cut(s)	543, 997, 1205, 2372
BstX2I	RGATCY	4 cut(s)	515, 646, 797, 2254
BstXI	CCANNNNNNTGG	2 cut(s)	387, 1750
BstYI	RGATCY	4 cut(s)	515, 646, 797, 2254
Bsu36I	CCTNAGG	1 cut(s)	1386
BsuI	GTATCC	1 cut(s)	1576
BsuRI	GGCC	7 cut(s)	772, 938, 1330, 1554, 1726, 2190, 2438
BtgI	CCRYGG	1 cut(s)	116
BtsCI	GGATG	6 cut(s)	303, 332, 354, 396, 1759, 2290
BtsI	GCAGTG	1 cut(s)	51
BtsIMutI	CAGTG	6 cut(s)	51, 1152, 1717, 1883, 1920, 2237
BveI	ACCTGC	1 cut(s)	2434
Cac8I	GCNNGC	2 cut(s)	536, 695
CaiI	CAGNNNCTG	1 cut(s)	50
CciI	TCATGA	1 cut(s)	2181
CfoI	GCGC	2 cut(s)	1320, 2281
Cfr10I	RCCGGY	2 cut(s)	151, 478
Cfr13I	GGNCC	4 cut(s)	1103, 2188, 2189, 2437
CseI	GACGC	1 cut(s)	537
Csp6I	GTAC	4 cut(s)	209, 1718, 2479, 2485
CspAI	ACCGGT	1 cut(s)	151
CspCI	CAANNNNNGTGG	2 cut(s)	1909, 1944
CviQI	GTAC	4 cut(s)	209, 1718, 2479, 2485
DdeI	CTNAG	7 cut(s)	356, 546, 1386, 1709, 1721, 1911, 2136
DrdI	GACNNNNNNGTC	1 cut(s)	409
DseDI	GACNNNNNNGTC	1 cut(s)	409
EaeI	YGGCCR	1 cut(s)	936
Eam1104I	CTCTTC	3 cut(s)	166, 1153, 1835
EarI	CTCTTC	3 cut(s)	166, 1153, 1835
EciI	GGCGGA	1 cut(s)	2264
Eco130I	CCWWGG	2 cut(s)	116, 1362
Eco147I	AGGCCT	1 cut(s)	1726
Eco24I	GRGCYC	4 cut(s)	1029, 2192, 2319, 2402
Eco31I	GGTCTC	1 cut(s)	2137
Eco32I	GATATC	1 cut(s)	713
Eco47I	GGWCC	1 cut(s)	1103
Eco47III	AGCGCT	1 cut(s)	1319
Eco57I	CTGAAG	1 cut(s)	1944
Eco81I	CCTNAGG	1 cut(s)	1386
Eco88I	CYCGRG	2 cut(s)	1544, 2393
Eco91I	GGTNACC	1 cut(s)	131
EcoO109I	RGGNCCY	2 cut(s)	2188, 2189
EcoO65I	GGTNACC	1 cut(s)	131
EcoRI	GAATTC	1 cut(s)	745
EcoRII	CCWGG	5 cut(s)	273, 565, 706, 1060, 2191
EcoRV	GATATC	1 cut(s)	713
EcoT14I	CCWWGG	2 cut(s)	116, 1362
EcoT22I	ATGCAT	1 cut(s)	2080
EcoT38I	GRGCYC	4 cut(s)	1029, 2192, 2319, 2402
ErhI	CCWWGG	2 cut(s)	116, 1362
FalI	AAGNNNNNCTT	2 cut(s)	2244, 2276
FaqI	GGGAC	5 cut(s)	281, 431, 570, 1404, 1499
FauNDI	CATATG	2 cut(s)	620, 1588
FblI	GTMKAC	2 cut(s)	496, 2012
Fnu4HI	GCNGC	4 cut(s)	532, 986, 1219, 2386
FokI	GGATG	6 cut(s)	310, 319, 361, 403, 1766, 2297
FriOI	GRGCYC	4 cut(s)	1029, 2192, 2319, 2402
Fsp4HI	GCNGC	4 cut(s)	532, 986, 1219, 2386
FspBI	CTAG	6 cut(s)	251, 500, 1308, 1764, 1935, 2219
GlaI	GCGC	2 cut(s)	1319, 2280
GluI	GCNGC	4 cut(s)	532, 986, 1219, 2386
GsaI	CCCAGC	1 cut(s)	348
GsuI	CTGGAG	2 cut(s)	690, 2118
HaeII	RGCGCY	1 cut(s)	1321
HaeIII	GGCC	7 cut(s)	772, 938, 1330, 1554, 1726, 2190, 2438
HapII	CCGG	4 cut(s)	152, 479, 1580, 1971
HgaI	GACGC	1 cut(s)	537
HhaI	GCGC	2 cut(s)	1320, 2281
Hin6I	GCGC	2 cut(s)	1318, 2279
HinP1I	GCGC	2 cut(s)	1318, 2279
HincII	GTYRAC	3 cut(s)	497, 687, 1990
HindII	GTYRAC	3 cut(s)	497, 687, 1990
HindIII	AAGCTT	1 cut(s)	1691
HinfI	GANTC	9 cut(s)	158, 269, 613, 739, 1242, 1253, 1592, 2090, 2296
HpaII	CCGG	4 cut(s)	152, 479, 1580, 1971
HphI	GGTGA	5 cut(s)	98, 412, 468, 534, 1569
Hpy166II	GTNNAC	8 cut(s)	157, 497, 687, 1093, 1210, 1799, 1990, 2013
Hpy188III	TCNNGA	9 cut(s)	370, 513, 650, 1422, 1634, 1971, 2055, 2182, 2300
Hpy8I	GTNNAC	8 cut(s)	157, 497, 687, 1093, 1210, 1799, 1990, 2013
HpyAV	CCTTC	9 cut(s)	91, 146, 1013, 1286, 1419, 1926, 2122, 2149, 2179
HpyCH4III	ACNGT	3 cut(s)	454, 1501, 2422
HpyF10VI	GCNNNNNNNGC	7 cut(s)	41, 100, 595, 699, 2048, 2314, 2406
HpyF3I	CTNAG	7 cut(s)	356, 546, 1386, 1709, 1721, 1911, 2136
HspAI	GCGC	2 cut(s)	1318, 2279
Kpn2I	TCCGGA	1 cut(s)	1970
LguI	GCTCTTC	1 cut(s)	1153
LmnI	GCTCC	3 cut(s)	1468, 1882, 2454
Lsp1109I	GCAGC	4 cut(s)	543, 997, 1205, 2372
LweI	GCATC	7 cut(s)	341, 512, 850, 961, 1198, 2065, 2087
MaeI	CTAG	6 cut(s)	251, 500, 1308, 1764, 1935, 2219
MaeIII	GTNAC	4 cut(s)	131, 400, 583, 2214
MfeI	CAATTG	2 cut(s)	1268, 2339
MflI	RGATCY	4 cut(s)	515, 646, 797, 2254
MhlI	GDGCHC	5 cut(s)	1029, 1095, 2192, 2319, 2402
MlsI	TGGCCA	1 cut(s)	938
MluNI	TGGCCA	1 cut(s)	938
MlyI	GAGTC	3 cut(s)	152, 263, 1586
MmeI	TCCRAC	2 cut(s)	192, 270
Mox20I	TGGCCA	1 cut(s)	938
Mph1103I	ATGCAT	1 cut(s)	2080
MroI	TCCGGA	1 cut(s)	1970
MscI	TGGCCA	1 cut(s)	938
MseI	TTAA	9 cut(s)	260, 759, 885, 1194, 1262, 1518, 1667, 1899, 2460
MslI	CAYNNNNRTG	1 cut(s)	855
Msp20I	TGGCCA	1 cut(s)	938
MspI	CCGG	4 cut(s)	152, 479, 1580, 1971
MspR9I	CCNGG	5 cut(s)	275, 567, 708, 1062, 2193
MunI	CAATTG	2 cut(s)	1268, 2339
Mva1269I	GAATGC	2 cut(s)	632, 2042
MvaI	CCWGG	5 cut(s)	275, 567, 708, 1062, 2193
MwoI	GCNNNNNNNGC	7 cut(s)	41, 100, 595, 699, 2048, 2314, 2406
NcoI	CCATGG	1 cut(s)	116
NdeI	CATATG	2 cut(s)	620, 1588
NlaIV	GGNNCC	3 cut(s)	564, 1104, 2190
NmuCI	GTSAC	1 cut(s)	400
NsiI	ATGCAT	1 cut(s)	2080
NspI	RCATGY	3 cut(s)	215, 663, 2011
PaeR7I	CTCGAG	1 cut(s)	2393
PagI	TCATGA	1 cut(s)	2181
PasI	CCCWGGG	1 cut(s)	274
PceI	AGGCCT	1 cut(s)	1726
PciI	ACATGT	1 cut(s)	2007
PciSI	GCTCTTC	1 cut(s)	1153
PctI	GAATGC	2 cut(s)	632, 2042
PfeI	GAWTC	6 cut(s)	613, 739, 1242, 1253, 2090, 2296
PfoI	TCCNGGA	1 cut(s)	706
PinAI	ACCGGT	1 cut(s)	151
PkrI	GCNGC	4 cut(s)	533, 987, 1220, 2387
PleI	GAGTC	3 cut(s)	152, 263, 1586
PpsI	GAGTC	3 cut(s)	152, 263, 1586
PscI	ACATGT	1 cut(s)	2007
PshAI	GACNNNNGTC	1 cut(s)	1396
PsiI	TTATAA	1 cut(s)	1233
Psp6I	CCWGG	5 cut(s)	273, 565, 706, 1060, 2191
PspEI	GGTNACC	1 cut(s)	131
PspFI	CCCAGC	1 cut(s)	344
PspGI	CCWGG	5 cut(s)	273, 565, 706, 1060, 2191
PspN4I	GGNNCC	3 cut(s)	564, 1104, 2190
PspOMI	GGGCCC	1 cut(s)	2188
PspPI	GGNCC	4 cut(s)	1103, 2188, 2189, 2437
PspXI	VCTCGAGB	1 cut(s)	2393
PsrI	GAACNNNNNNTAC	2 cut(s)	2160, 2192
PstI	CTGCAG	2 cut(s)	2167, 2445
PstNI	CAGNNNCTG	1 cut(s)	50
PsuI	RGATCY	4 cut(s)	515, 646, 797, 2254
RsaI	GTAC	4 cut(s)	210, 1719, 2480, 2486
RsaNI	GTAC	4 cut(s)	209, 1718, 2479, 2485
RseI	CAYNNNNRTG	1 cut(s)	855
SalI	GTCGAC	1 cut(s)	495
SapI	GCTCTTC	1 cut(s)	1153
SaqAI	TTAA	9 cut(s)	260, 759, 885, 1194, 1262, 1518, 1667, 1899, 2460
SatI	GCNGC	4 cut(s)	532, 986, 1219, 2386
Sau96I	GGNCC	4 cut(s)	1103, 2188, 2189, 2437
SbfI	CCTGCAGG	1 cut(s)	2445
ScaI	AGTACT	1 cut(s)	2480
SchI	GAGTC	3 cut(s)	152, 263, 1586
ScrFI	CCNGG	5 cut(s)	275, 567, 708, 1062, 2193
SdaI	CCTGCAGG	1 cut(s)	2445
SduI	GDGCHC	5 cut(s)	1029, 1095, 2192, 2319, 2402
SfaNI	GCATC	7 cut(s)	341, 512, 850, 961, 1198, 2065, 2087
SfcI	CTRYAG	3 cut(s)	2163, 2418, 2441
Sfr274I	CTCGAG	1 cut(s)	2393
SinI	GGWCC	1 cut(s)	1103
SlaI	CTCGAG	1 cut(s)	2393
SmiMI	CAYNNNNRTG	1 cut(s)	855
SmlI	CTYRAG	2 cut(s)	1213, 2393
SmoI	CTYRAG	2 cut(s)	1213, 2393
Sse8387I	CCTGCAGG	1 cut(s)	2445
SseBI	AGGCCT	1 cut(s)	1726
SsiI	CCGC	3 cut(s)	330, 1106, 2249
SspI	AATATT	1 cut(s)	1793
SspMI	CTAG	6 cut(s)	251, 500, 1308, 1764, 1935, 2219
StuI	AGGCCT	1 cut(s)	1726
StyD4I	CCNGG	5 cut(s)	273, 565, 706, 1060, 2191
StyI	CCWWGG	2 cut(s)	116, 1362
TaaI	ACNGT	3 cut(s)	454, 1501, 2422
TaqI	TCGA	8 cut(s)	70, 469, 496, 1038, 1240, 2088, 2113, 2394
TatI	WGTACW	3 cut(s)	1717, 2478, 2484
TfiI	GAWTC	6 cut(s)	613, 739, 1242, 1253, 2090, 2296
Tru1I	TTAA	9 cut(s)	260, 759, 885, 1194, 1262, 1518, 1667, 1899, 2460
Tru9I	TTAA	9 cut(s)	260, 759, 885, 1194, 1262, 1518, 1667, 1899, 2460
TscAI	CASTG	6 cut(s)	51, 1159, 1717, 1890, 1927, 2237
TseFI	GTSAC	1 cut(s)	400
TseI	GCWGC	4 cut(s)	531, 985, 1218, 2385
Tsp45I	GTSAC	1 cut(s)	400
TspGWI	ACGGA	1 cut(s)	919
TspRI	CASTG	6 cut(s)	51, 1159, 1717, 1890, 1927, 2237
VneI	GTGCAC	1 cut(s)	1091
VpaK11BI	GGWCC	1 cut(s)	1103
XapI	RAATTY	4 cut(s)	484, 745, 1345, 2362
XceI	RCATGY	3 cut(s)	215, 663, 2011
XhoI	CTCGAG	1 cut(s)	2393
XmiI	GTMKAC	2 cut(s)	496, 2012
XspI	CTAG	6 cut(s)	251, 500, 1308, 1764, 1935, 2219
ZrmI	AGTACT	1 cut(s)	2480
Zsp2I	ATGCAT	1 cut(s)	2080

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.