Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0002753

Physical Location & Seq

Genomic Coordinates Reverse (-)

48807724 .. 48811671

Gene Structure 3948 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G023100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1749 bp

ATGGTTAAGGAAGAAAGGCCACAAATTACAAGGAGAGCAACCAGATCCTCCAATTTGGCTAAAAGTGTAGTTGAAGAAACAGATACAAGCAGTCAACACCCAAATGATGCTACTTTGAGTTTAGACATGGTTGAAGAAGAAAGCCCACAAATCACAAGGAGAACAACCCGATCCTCTGCTTTGGCCAAATGTTCAAACAATGTAGTTGAAGAAACAAAAGCAAATAGTCAGCAGCAACTGACTTTAGATGACCTTGTGATTGGAGAAGAAGGAGAGTCCTTTAGCTTCGCTGATCTGGTGTCTAGTTTTCCTGGTAGAGATAGTCAGATCAAAGAGCTTGTGCACCTTTTGGGTCCGCAGAACTCTCCCATGCTTCCTATATTTATATATGGAGGCTCTTCCACTGGAAAAACTAGTATTGTTCTACAAACATTTAGGCATCTTAAACGACCTATTGTTTACCTAAGCTGCCTTACCTGTTATAATCCTCGAATATTGTTTGAATCCTTATTGAATCAATTGTTCCTTCACCAAAAGAATGCAGAAAATGGTTATTCTAGTGCAAAGCGCTGTGAAAAACCATCTGATTTTGTGAATTTTCTTCGCGAAGCCTTGGTTAGCATTCTTGGTAATCCTCAGGGACAAAAGTCAGGATTGAGTAGCAAAAGGTCAGGAAAGGCTAGTGGAAATATGATCTACTTGATTTTTGACAATTTGGAGCGTGTCAAAGGGTGGGACAAAGGTTCTACTGTGTTACCTTTGTTGTTTAATCTCTATGATACTCTGAAAAAGCCCGAGGTCGGTCTTATCTTCATCAGCAATACCTCACCGGATACATATGACTCAAATATGGGTTATGTTGAGCCTAACCCTCTATATCTTCCTGATTATACGGATGATGATCTTGGTCAAATCTTAATGAGAAACCAAGCAAACAAAAAGCTTTATACCTCTTTTCTCAGTGTTGTACTAAGGCCTTTCAGTAGAATTACCAGACGAGTGGATGAATTGTCTAGTGCCTTTTCACTATTATTCAGAAAATATTGTGAACCTGTTACTGATCTTGGGGTTGTTCCCAATGAAGAAATGAAGAGAAAGTTGTTTAGTCAATTTCAGCCCCATATTGCTCCTGCACTGAATGAGATATTTAAGGTGTCAACTCAGCCTTCCACTGAAGTTGAAGCTAGGGAGAGAAAGCCAAAGGGTAGCACAAGGAAATCCGGAGGTTATTATGAAGCTGTTGACCAGTTGGATTTTCACATGTCTACTTCTGCCAAGTATCTTCTTATTTCAGCATTCCTTGCTTCAAGAAATCCAGCTACCCTTGATGCATCATTGTTTGATTCTACTGGAGGTTCTAATAATCGAAAACGAAAGAGGAAGGTCTCTGAGAAGTCCATGGAACAGAAGGAAACTGCAGAACAGGAATTACTCATGAAGGGCCCCGGTACATTCGCAATGGAGAGGTTACTAGCCATATTCCAGTGTATTACATCTGTGGCGGAAGATCCACTTGATGATGAGGAACAAGCGCAGGATGGGTTAGGCATTCAAGATGGGCATTGTGGGCTCATGTCTGATGTCCTCTTACAATTGGCTAGTCTCTGTGGTGCAAATTTTATTGTCAAAGGAGGAAGCTGCCCACTGGAGGGCTCAACTCGCTATCGTTCTACAGTTAGTGAAGATATGGCCCTGCAGGTAGCAAGGAGCATTAAGTTTCCTCTCTCAAAGTATTTGTACAGAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000808 GO:0003674 GO:0003676 GO:0003677 GO:0003688 GO:0003690 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005664 GO:0005694 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009743 GO:0009744 GO:0009987 GO:0010033 GO:0031974 GO:0031981 GO:0032991 GO:0034285 GO:0034641 GO:0034645 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0050896 GO:0070013 GO:0071704 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901576 GO:1901700 GO:1990837

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 map04110 map04111 map04113

Pfam Domains

Protein Families

AAA_16 ORC5_C

Protein Analysis

582

Amino Acids

65.02

Weight (kDa)

7.52

Isoelectric Point (pI)

47.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_16	PF13191	103 - 245	3e-12	AAA ATPase domain
ATPase_2	PF01637	105 - 276	4.4e-06	ATPase domain predominantly from Archaea
ORC5_lid	PF21639	316 - 369	2.8e-11	ORC5, lid domain
ORC5_C	PF14630	421 - 579	4e-39	Origin recognition complex (ORC) subunit 5 C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002753)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29910 AT4G29910
fragaria_vesca	FvH4_7g14490 FvH4_7g14490 FvH4_7g14490 FvH4_7g14490 FvH4_7g14490
malus_domestica	MD01G1065000.v1.1 MD07G1135400.v1.1
prunus_persica	Prupe.2G172000_v2.0.a1 Prupe.2G172000_v2.0.a1 Prupe.2G172000_v2.0.a1
pyrus_communis	pycom01g09430 pycom07g13260
rosa_chinensis	RchiOBHm_Chr1g0355901 RchiOBHm_Chr2g0161261
rosa_laevigata	RLG00000021273 RLG00000028117
rosa_multiflora	Rmu_sc0004705.1_g000017 Rmu_sc0009869.1_g000009
rosa_roxburghii	Rroxscaffold_2G00088970 Rroxscaffold_2G00110130 Rroxscaffold_4G00299930
rosa_rugosa	Rorug01G0248300 Rorug01G0248400 Rorug01G0248500 Rorug02G0487300 Rorug02G0487400 Rorug02G0487500
rosa_samantha	Rh1AG261600 Rh1BG229700 Rh1CG244700 Rh1DG257700 Rh2AG554200 Rh2BG567600 Rh2CG538100 Rh2DG577000
rosa_wichuraiana	Rw1G023100 Rw2G045850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	483
Acc36I	ACCTGC	1 cut(s)	1687
AccI	GTMKAC	1 cut(s)	1265
AccII	CGCG	1 cut(s)	606
AccIII	TCCGGA	1 cut(s)	1220
AciI	CCGC	2 cut(s)	356, 1502
AclWI	GGATC	3 cut(s)	39, 165, 1502
AcoI	YGGCCR	1 cut(s)	183
AcsI	RAATTY	2 cut(s)	595, 1615
AcuI	CTGAAG	1 cut(s)	1194
AfaI	GTAC	3 cut(s)	969, 1450, 1739
AfeI	AGCGCT	1 cut(s)	569
AfiI	CCNNNNNNNGG	3 cut(s)	800, 1648, 1649
AflIII	ACRYGT	1 cut(s)	1260
AgsI	TTSAA	9 cut(s)	74, 134, 195, 209, 503, 514, 1181, 1308, 1553
AhlI	ACTAGT	1 cut(s)	413
AjnI	CCWGG	1 cut(s)	310
AluBI	AGCT	8 cut(s)	285, 337, 468, 943, 1184, 1238, 1319, 1638
AluI	AGCT	8 cut(s)	285, 337, 468, 943, 1184, 1238, 1319, 1638
Alw21I	GWGCWC	1 cut(s)	345
Alw26I	GTCTC	2 cut(s)	1390, 1607
Alw44I	GTGCAC	1 cut(s)	341
AlwI	GGATC	3 cut(s)	39, 165, 1502
AlwNI	CAGNNNCTG	1 cut(s)	238
Ama87I	CYCGRG	1 cut(s)	794
Aor13HI	TCCGGA	1 cut(s)	1220
Aor51HI	AGCGCT	1 cut(s)	569
AoxI	GGCC	5 cut(s)	17, 183, 974, 1441, 1689
ApaI	GGGCCC	1 cut(s)	1445
ApaLI	GTGCAC	1 cut(s)	341
ApeKI	GCWGC	3 cut(s)	232, 468, 1638
ApoI	RAATTY	2 cut(s)	595, 1615
AspLEI	GCGC	2 cut(s)	570, 1534
AspS9I	GGNCC	4 cut(s)	353, 1441, 1442, 1690
AsuC2I	CCSGG	1 cut(s)	1446
AsuHPI	GGTGA	2 cut(s)	521, 819
AvaI	CYCGRG	1 cut(s)	794
AvaII	GGWCC	1 cut(s)	353
AxyI	CCTNAGG	1 cut(s)	636
BaeGI	GKGCMC	2 cut(s)	345, 1445
BalI	TGGCCA	1 cut(s)	185
BanII	GRGCYC	3 cut(s)	1445, 1572, 1655
Bbv12I	GWGCWC	1 cut(s)	345
BbvI	GCAGC	3 cut(s)	244, 455, 1625
BccI	CCATC	3 cut(s)	589, 1532, 1550
BciT130I	CCWGG	1 cut(s)	312
BciVI	GTATCC	1 cut(s)	826
BcnI	CCSGG	1 cut(s)	1446
BcoDI	GTCTC	2 cut(s)	1390, 1607
BcuI	ACTAGT	1 cut(s)	413
BfaI	CTAG	8 cut(s)	303, 414, 558, 681, 1014, 1185, 1472, 1599
BfmI	CTRYAG	3 cut(s)	1416, 1671, 1694
BfoI	RGCGCY	1 cut(s)	571
BfuAI	ACCTGC	1 cut(s)	1687
BfuI	GTATCC	1 cut(s)	826
BisI	GCNGC	3 cut(s)	233, 469, 1639
BlsI	GCNGC	3 cut(s)	234, 470, 1640
Bme1390I	CCNGG	2 cut(s)	312, 1446
Bme18I	GGWCC	1 cut(s)	353
BmeT110I	CYCGRG	1 cut(s)	794
BmgT120I	GGNCC	4 cut(s)	353, 1441, 1442, 1690
BmiI	GGNNCC	3 cut(s)	354, 1443, 1444
BmrFI	CCNGG	2 cut(s)	312, 1446
BmsI	GCATC	4 cut(s)	97, 448, 1318, 1340
BoxI	GACNNNNGTC	1 cut(s)	646
BpmI	CTGGAG	2 cut(s)	1371, 1667
Bpu10I	CCTNAGC	1 cut(s)	464
BpuMI	CCSGG	1 cut(s)	1446
BsaBI	GATNNNNATC	1 cut(s)	900
BsaI	GGTCTC	1 cut(s)	1390
BsaJI	CCNNGG	4 cut(s)	612, 795, 1398, 1444
BsaWI	WCCGGW	2 cut(s)	829, 1220
Bsc4I	CCNNNNNNNGG	3 cut(s)	800, 1648, 1649
Bse1I	ACTGG	5 cut(s)	409, 1246, 1354, 1483, 1650
Bse21I	CCTNAGG	1 cut(s)	636
Bse3DI	GCAATG	1 cut(s)	1464
Bse8I	GATNNNNATC	1 cut(s)	900
BseAI	TCCGGA	1 cut(s)	1220
BseBI	CCWGG	1 cut(s)	312
BseDI	CCNNGG	4 cut(s)	612, 795, 1398, 1444
BseGI	GGATG	3 cut(s)	901, 1009, 1543
BseJI	GATNNNNATC	1 cut(s)	900
BseLI	CCNNNNNNNGG	3 cut(s)	800, 1648, 1649
BseMI	GCAATG	1 cut(s)	1464
BseMII	CTCAG	4 cut(s)	650, 973, 1175, 1380
BseNI	ACTGG	5 cut(s)	409, 1246, 1354, 1483, 1650
BseSI	GKGCMC	2 cut(s)	345, 1445
BseXI	GCAGC	3 cut(s)	244, 455, 1625
BsgI	GTGCAG	1 cut(s)	1116
Bsh1236I	CGCG	1 cut(s)	606
BshFI	GGCC	5 cut(s)	19, 185, 976, 1443, 1691
BsiHKAI	GWGCWC	1 cut(s)	345
BsiHKCI	CYCGRG	1 cut(s)	794
BsiSI	CCGG	3 cut(s)	830, 1221, 1446
BslFI	GGGAC	2 cut(s)	654, 749
BslI	CCNNNNNNNGG	3 cut(s)	800, 1648, 1649
BsmAI	GTCTC	2 cut(s)	1390, 1607
BsmFI	GGGAC	2 cut(s)	654, 749
BsmI	GAATGC	4 cut(s)	544, 621, 1295, 1548
BsnI	GGCC	5 cut(s)	19, 185, 976, 1443, 1691
Bso31I	GGTCTC	1 cut(s)	1390
BsoBI	CYCGRG	1 cut(s)	794
Bsp120I	GGGCCC	1 cut(s)	1441
Bsp1286I	GDGCHC	4 cut(s)	345, 1445, 1572, 1655
Bsp13I	TCCGGA	1 cut(s)	1220
Bsp1407I	TGTACA	1 cut(s)	1737
Bsp143I	GATC	8 cut(s)	44, 170, 292, 327, 693, 901, 1060, 1507
Bsp19I	CCATGG	1 cut(s)	1398
Bsp68I	TCGCGA	1 cut(s)	606
BspACI	CCGC	2 cut(s)	356, 1502
BspANI	GGCC	5 cut(s)	19, 185, 976, 1443, 1691
BspCNI	CTCAG	4 cut(s)	649, 972, 1174, 1381
BspEI	TCCGGA	1 cut(s)	1220
BspFNI	CGCG	1 cut(s)	606
BspHI	TCATGA	1 cut(s)	1434
BspLI	GGNNCC	3 cut(s)	354, 1443, 1444
BspMAI	CTGCAG	2 cut(s)	1420, 1698
BspMI	ACCTGC	1 cut(s)	1687
BspPI	GGATC	3 cut(s)	39, 165, 1502
BspQI	GCTCTTC	1 cut(s)	403
BspTNI	GGTCTC	1 cut(s)	1390
BsrDI	GCAATG	1 cut(s)	1464
BsrGI	TGTACA	1 cut(s)	1737
BsrI	ACTGG	5 cut(s)	409, 1246, 1354, 1483, 1650
BssECI	CCNNGG	4 cut(s)	612, 795, 1398, 1444
BssMI	GATC	8 cut(s)	44, 170, 292, 327, 693, 901, 1060, 1507
BssT1I	CCWWGG	2 cut(s)	612, 1398
Bst2UI	CCWGG	1 cut(s)	312
Bst4CI	ACNGT	2 cut(s)	751, 1675
Bst6I	CTCTTC	2 cut(s)	403, 1085
BstAPI	GCANNNNNTGC	1 cut(s)	1301
BstAUI	TGTACA	1 cut(s)	1737
BstDEI	CTNAG	6 cut(s)	464, 636, 959, 971, 1161, 1389
BstDSI	CCRYGG	1 cut(s)	1398
BstF5I	GGATG	3 cut(s)	901, 1009, 1543
BstFNI	CGCG	1 cut(s)	606
BstH2I	RGCGCY	1 cut(s)	571
BstHHI	GCGC	2 cut(s)	570, 1534
BstKTI	GATC	8 cut(s)	47, 173, 295, 330, 696, 904, 1063, 1510
BstMAI	GTCTC	2 cut(s)	1390, 1607
BstMBI	GATC	8 cut(s)	44, 170, 292, 327, 693, 901, 1060, 1507
BstMWI	GCNNNNNNNGC	3 cut(s)	1301, 1567, 1659
BstNI	CCWGG	1 cut(s)	312
BstNSI	RCATGY	1 cut(s)	1264
BstPAI	GACNNNNGTC	1 cut(s)	646
BstSCI	CCNGG	2 cut(s)	310, 1444
BstSFI	CTRYAG	3 cut(s)	1416, 1671, 1694
BstSLI	GKGCMC	2 cut(s)	345, 1445
BstUI	CGCG	1 cut(s)	606
BstV1I	GCAGC	3 cut(s)	244, 455, 1625
BstX2I	RGATCY	2 cut(s)	44, 1507
BstXI	CCANNNNNNTGG	1 cut(s)	1000
BstYI	RGATCY	2 cut(s)	44, 1507
Bsu36I	CCTNAGG	1 cut(s)	636
BsuI	GTATCC	1 cut(s)	826
BsuRI	GGCC	5 cut(s)	19, 185, 976, 1443, 1691
BtgI	CCRYGG	1 cut(s)	1398
BtsCI	GGATG	3 cut(s)	901, 1009, 1543
BtsIMutI	CAGTG	6 cut(s)	402, 967, 1133, 1170, 1490, 1643
BtuMI	TCGCGA	1 cut(s)	606
BveI	ACCTGC	1 cut(s)	1687
CaiI	CAGNNNCTG	1 cut(s)	238
CciI	TCATGA	1 cut(s)	1434
CfoI	GCGC	2 cut(s)	570, 1534
Cfr13I	GGNCC	4 cut(s)	353, 1441, 1442, 1690
Csp6I	GTAC	3 cut(s)	968, 1449, 1738
CspCI	CAANNNNNGTGG	2 cut(s)	1159, 1194
CviAII	CATG	6 cut(s)	127, 370, 1261, 1399, 1435, 1573
CviQI	GTAC	3 cut(s)	968, 1449, 1738
DdeI	CTNAG	6 cut(s)	464, 636, 959, 971, 1161, 1389
DpnI	GATC	8 cut(s)	46, 172, 294, 329, 695, 903, 1062, 1509
DpnII	GATC	8 cut(s)	44, 170, 292, 327, 693, 901, 1060, 1507
EaeI	YGGCCR	1 cut(s)	183
Eam1104I	CTCTTC	2 cut(s)	403, 1085
EarI	CTCTTC	2 cut(s)	403, 1085
EciI	GGCGGA	1 cut(s)	1517
Eco130I	CCWWGG	2 cut(s)	612, 1398
Eco147I	AGGCCT	1 cut(s)	976
Eco24I	GRGCYC	3 cut(s)	1445, 1572, 1655
Eco31I	GGTCTC	1 cut(s)	1390
Eco47I	GGWCC	1 cut(s)	353
Eco47III	AGCGCT	1 cut(s)	569
Eco57I	CTGAAG	1 cut(s)	1194
Eco81I	CCTNAGG	1 cut(s)	636
Eco88I	CYCGRG	1 cut(s)	794
EcoO109I	RGGNCCY	2 cut(s)	1441, 1442
EcoRII	CCWGG	1 cut(s)	310
EcoT14I	CCWWGG	2 cut(s)	612, 1398
EcoT22I	ATGCAT	1 cut(s)	1333
EcoT38I	GRGCYC	3 cut(s)	1445, 1572, 1655
ErhI	CCWWGG	2 cut(s)	612, 1398
FaeI	CATG	6 cut(s)	130, 373, 1264, 1402, 1438, 1576
FalI	AAGNNNNNCTT	2 cut(s)	1497, 1529
FaqI	GGGAC	2 cut(s)	654, 749
FatI	CATG	6 cut(s)	126, 369, 1260, 1398, 1434, 1572
FauNDI	CATATG	1 cut(s)	838
FblI	GTMKAC	1 cut(s)	1265
Fnu4HI	GCNGC	3 cut(s)	233, 469, 1639
FokI	GGATG	3 cut(s)	908, 1016, 1550
FriOI	GRGCYC	3 cut(s)	1445, 1572, 1655
Fsp4HI	GCNGC	3 cut(s)	233, 469, 1639
FspBI	CTAG	8 cut(s)	303, 414, 558, 681, 1014, 1185, 1472, 1599
GlaI	GCGC	2 cut(s)	569, 1533
GluI	GCNGC	3 cut(s)	233, 469, 1639
GsuI	CTGGAG	2 cut(s)	1371, 1667
HaeII	RGCGCY	1 cut(s)	571
HaeIII	GGCC	5 cut(s)	19, 185, 976, 1443, 1691
HapII	CCGG	3 cut(s)	830, 1221, 1446
HhaI	GCGC	2 cut(s)	570, 1534
Hin1II	CATG	6 cut(s)	130, 373, 1264, 1402, 1438, 1576
Hin6I	GCGC	2 cut(s)	568, 1532
HinP1I	GCGC	2 cut(s)	568, 1532
HincII	GTYRAC	3 cut(s)	95, 1158, 1243
HindII	GTYRAC	3 cut(s)	95, 1158, 1243
HindIII	AAGCTT	1 cut(s)	941
HinfI	GANTC	5 cut(s)	275, 503, 514, 842, 1343
HpaII	CCGG	3 cut(s)	830, 1221, 1446
HphI	GGTGA	2 cut(s)	521, 819
Hpy166II	GTNNAC	7 cut(s)	95, 343, 460, 1049, 1158, 1243, 1266
Hpy188I	TCNGA	6 cut(s)	327, 586, 786, 1037, 1390, 1579
Hpy188III	TCNNGA	8 cut(s)	605, 651, 672, 884, 1221, 1308, 1435, 1553
Hpy8I	GTNNAC	7 cut(s)	95, 343, 460, 1049, 1158, 1243, 1266
HpyAV	CCTTC	6 cut(s)	263, 536, 1176, 1375, 1402, 1432
HpyCH4III	ACNGT	2 cut(s)	751, 1675
HpyCH4V	TGCA	8 cut(s)	343, 542, 563, 1133, 1331, 1418, 1613, 1696
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1301, 1567, 1659
HpyF3I	CTNAG	6 cut(s)	464, 636, 959, 971, 1161, 1389
Hsp92II	CATG	6 cut(s)	130, 373, 1264, 1402, 1438, 1576
HspAI	GCGC	2 cut(s)	568, 1532
Kpn2I	TCCGGA	1 cut(s)	1220
Kzo9I	GATC	8 cut(s)	44, 170, 292, 327, 693, 901, 1060, 1507
LguI	GCTCTTC	1 cut(s)	403
LmnI	GCTCC	3 cut(s)	718, 1132, 1707
Lsp1109I	GCAGC	3 cut(s)	244, 455, 1625
LweI	GCATC	4 cut(s)	97, 448, 1318, 1340
MaeI	CTAG	8 cut(s)	303, 414, 558, 681, 1014, 1185, 1472, 1599
MaeIII	GTNAC	3 cut(s)	753, 1054, 1467
MalI	GATC	8 cut(s)	46, 172, 294, 329, 695, 903, 1062, 1509
MboI	GATC	8 cut(s)	44, 170, 292, 327, 693, 901, 1060, 1507
MfeI	CAATTG	2 cut(s)	518, 1592
MflI	RGATCY	2 cut(s)	44, 1507
MhlI	GDGCHC	4 cut(s)	345, 1445, 1572, 1655
MlsI	TGGCCA	1 cut(s)	185
MluNI	TGGCCA	1 cut(s)	185
MlyI	GAGTC	2 cut(s)	284, 836
MmeI	TCCRAC	1 cut(s)	1230
Mox20I	TGGCCA	1 cut(s)	185
Mph1103I	ATGCAT	1 cut(s)	1333
MroI	TCCGGA	1 cut(s)	1220
MscI	TGGCCA	1 cut(s)	185
MseI	TTAA	6 cut(s)	6, 444, 768, 917, 1149, 1713
MslI	CAYNNNNRTG	1 cut(s)	102
Msp20I	TGGCCA	1 cut(s)	185
MspI	CCGG	3 cut(s)	830, 1221, 1446
MspR9I	CCNGG	2 cut(s)	312, 1446
MunI	CAATTG	2 cut(s)	518, 1592
Mva1269I	GAATGC	4 cut(s)	544, 621, 1295, 1548
MvaI	CCWGG	1 cut(s)	312
MvnI	CGCG	1 cut(s)	606
MwoI	GCNNNNNNNGC	3 cut(s)	1301, 1567, 1659
NciI	CCSGG	1 cut(s)	1446
NcoI	CCATGG	1 cut(s)	1398
NdeI	CATATG	1 cut(s)	838
NdeII	GATC	8 cut(s)	44, 170, 292, 327, 693, 901, 1060, 1507
NlaIII	CATG	6 cut(s)	130, 373, 1264, 1402, 1438, 1576
NlaIV	GGNNCC	3 cut(s)	354, 1443, 1444
NruI	TCGCGA	1 cut(s)	606
NsiI	ATGCAT	1 cut(s)	1333
NspI	RCATGY	1 cut(s)	1264
PagI	TCATGA	1 cut(s)	1434
PceI	AGGCCT	1 cut(s)	976
PciI	ACATGT	1 cut(s)	1260
PciSI	GCTCTTC	1 cut(s)	403
PctI	GAATGC	4 cut(s)	544, 621, 1295, 1548
PfeI	GAWTC	3 cut(s)	503, 514, 1343
PkrI	GCNGC	3 cut(s)	234, 470, 1640
PleI	GAGTC	2 cut(s)	283, 836
PpsI	GAGTC	2 cut(s)	283, 836
PscI	ACATGT	1 cut(s)	1260
PshAI	GACNNNNGTC	1 cut(s)	646
PsiI	TTATAA	1 cut(s)	483
Psp6I	CCWGG	1 cut(s)	310
PspGI	CCWGG	1 cut(s)	310
PspN4I	GGNNCC	3 cut(s)	354, 1443, 1444
PspOMI	GGGCCC	1 cut(s)	1441
PspPI	GGNCC	4 cut(s)	353, 1441, 1442, 1690
PsrI	GAACNNNNNNTAC	2 cut(s)	1413, 1445
PstI	CTGCAG	2 cut(s)	1420, 1698
PstNI	CAGNNNCTG	1 cut(s)	238
PsuI	RGATCY	2 cut(s)	44, 1507
RruI	TCGCGA	1 cut(s)	606
RsaI	GTAC	3 cut(s)	969, 1450, 1739
RsaNI	GTAC	3 cut(s)	968, 1449, 1738
RseI	CAYNNNNRTG	1 cut(s)	102
SapI	GCTCTTC	1 cut(s)	403
SaqAI	TTAA	6 cut(s)	6, 444, 768, 917, 1149, 1713
SatI	GCNGC	3 cut(s)	233, 469, 1639
Sau3AI	GATC	8 cut(s)	44, 170, 292, 327, 693, 901, 1060, 1507
Sau96I	GGNCC	4 cut(s)	353, 1441, 1442, 1690
SbfI	CCTGCAGG	1 cut(s)	1698
SchI	GAGTC	2 cut(s)	284, 836
ScrFI	CCNGG	2 cut(s)	312, 1446
SdaI	CCTGCAGG	1 cut(s)	1698
SduI	GDGCHC	4 cut(s)	345, 1445, 1572, 1655
SfaNI	GCATC	4 cut(s)	97, 448, 1318, 1340
SfcI	CTRYAG	3 cut(s)	1416, 1671, 1694
SinI	GGWCC	1 cut(s)	353
SmiMI	CAYNNNNRTG	1 cut(s)	102
SpeI	ACTAGT	1 cut(s)	413
Sse8387I	CCTGCAGG	1 cut(s)	1698
SseBI	AGGCCT	1 cut(s)	976
SsiI	CCGC	2 cut(s)	356, 1502
SspI	AATATT	2 cut(s)	495, 1043
SspMI	CTAG	8 cut(s)	303, 414, 558, 681, 1014, 1185, 1472, 1599
StuI	AGGCCT	1 cut(s)	976
StyD4I	CCNGG	2 cut(s)	310, 1444
StyI	CCWWGG	2 cut(s)	612, 1398
TaaI	ACNGT	2 cut(s)	751, 1675
TaqI	TCGA	2 cut(s)	490, 1366
TaqII	GACCGA	1 cut(s)	791
TatI	WGTACW	2 cut(s)	967, 1737
TfiI	GAWTC	3 cut(s)	503, 514, 1343
Tru1I	TTAA	6 cut(s)	6, 444, 768, 917, 1149, 1713
Tru9I	TTAA	6 cut(s)	6, 444, 768, 917, 1149, 1713
TscAI	CASTG	6 cut(s)	409, 967, 1140, 1177, 1490, 1650
TseI	GCWGC	3 cut(s)	232, 468, 1638
TspDTI	ATGAA	6 cut(s)	802, 1020, 1095, 1103, 1248, 1451
TspGWI	ACGGA	1 cut(s)	908
TspRI	CASTG	6 cut(s)	409, 967, 1140, 1177, 1490, 1650
VneI	GTGCAC	1 cut(s)	341
VpaK11BI	GGWCC	1 cut(s)	353
XapI	RAATTY	2 cut(s)	595, 1615
XceI	RCATGY	1 cut(s)	1264
XmiI	GTMKAC	1 cut(s)	1265
XspI	CTAG	8 cut(s)	303, 414, 558, 681, 1014, 1185, 1472, 1599
Zsp2I	ATGCAT	1 cut(s)	1333

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.