Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0002753

Physical Location & Seq

Genomic Coordinates Forward (+)

80727599 .. 80777220

Gene Structure 49622 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG577000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2997 bp

ATGGTGAGAACTCCTTTCTGTGATGAAAGGGGAGTGAAGAAAGGTACATGGACTCCGGAGGAAGATAGGAAGCTCATAGCTTATGTTACTAGGTATGGCTGCTGGAATTGGCGCCAACTCCCCAAGTTTGCTGGGCTTTCAAGGTGTGGGAAGAGTTGCAGGCTGAGGTGGCTGAATTACTTGAGGCCGAACATCAAGAGAGGAAACTACACCAATGAAGAAGAGGAAATAATAATCAGGCTTCACGAAAAGCTGGGGAACAAATGGTCTGCGATTGCGGCACAGCTTCAGGGAAGAACTGACAATGAGGTTAAGAATCACTGGCACACAAACCTCAAGAAACGTTTAATGATGAACAAGCACAAGGCGTCCTCAGTTTCAATAACACATGATGATCATCATGAAGAAGTAGTACCGAAATCATCGGATCAGCCAAAGACTACTGCTACTGCTGATTTCGCAAATGCAGAAACAACTGTTGATCAAATTCCCCAAATTGATTATTTTGATTACCCAGAACTGTGGTCGCCACAGCCATCATCGAGTGAGGTCTCGTCCATGACCACCGATACTGCGGTTGCGATTACTGCCGCAGATGGTTTGGTTGTAGTGGACAATGCTACTTCAGCTGCCATGGAAACAATTTATGGAGATTTCGGAGATAATTTCTGGACAGAACCATTTCTGGCTGATAATTCCTACAACCCAACTGGTTTTTATACACCCTTGATGGAGCCTGAGTTTCTGCTGACGGAAGATTTCAGTGGATTGTTGTTGGACGAGAACCTGGTGGCACAAGCTATCAAGCTACTGATAGAAACGAAACATGTTCTAATATCAAATTTATCAGCTATCGGAAATGGAACGGGTCTAGTGTTAGACAGTGCGAGGAAGACGATCAAAGAGAGAGAGAGAGATGCAAATGCTCTAACATGGATCCCAACGAGACCGCACAGAAGCTTCTTCCCCTTCAGGCTAGAATTGTGGGGAGACATGGAGTCAGAGACTATGGTCTGTACCACAGATCAATGTGTTGAACAAGAGCCCCAGTACACACATATTGACCAGAATGAATTCTCACAGAGAAGGCACAAGAAGCAGGACGAGGAGGAGATTCTCATATGTGAATGCAAGTGTGATGAAAAGGATCTTGAAAGTGCATGTGGAGAAAGGTGCTTGAATGTGTTGACCAGCACGCAATGCACTCCTGGATATTGCCCTTGTGGTGTCTATTGTAAGAATCAGAATTCAGACATGGTTAAGGAAGAAATGCCACAAATTACAAGGAGAGCAACCAGATCCTCCGCTTTGGCTAAAAGTGTAGTTGAAGAAACAGATACAAGCAGTCAGCACCCAAATGATGCTTCTGCGAGTTCAGACATTATTATAGAAGAAAGCCCACAAATTTCAAGGAGAACAACCCGATCCTCCGTTTTGGCCAAATGTTCAAACAATGTAGTTGAAGAAACAGATGCAAATAGTCAGCAGCAACCGACTTTAGATGACCTTGTGATTGGAGAAGGAGAGTCCTTTAGCTTCGATGATCTGGTGTCTAGTTTTCCTGGTAAAGAAAGTCAGATCAAAGAGCTTGTGCACCTTTTGGGTCCGCAGAACTCTCCCATGCTTCCTATATTTATATATGGAGGCTCTTCCACTGGAAAAACCAGTATTGTTCTACAAACATTTAGGCATCTTAAACGACCTTTTGTTTACTCAAGCTGCCTTACCTGTTATAATCCTCGAATCTTGTTTGAATCCATATTAAATGAATTGTTCCTTCACCGAAAGAAAGCAGAAAATGGTTATTCTAGTGCAAAGCGCTGTGAAAGGCCATCTGATTTTGTGAATTTTCTTCGCGAAGCCTTGGTTAGCATTATTGGTAATCCTCAGGGACAAAAGTCAGGATTGAGTAGCAAAAGGTCAGGAAAGGCTAATGGAAATATGATCTACTTGATTTTTGACAATTTGGAGCGTGTCAAAGGGTGGGACAAAGGTTCTACTGTGTTACCTTTGTTGTTTAACCTCTATGACACTCTGAAAATGCCCGAGGTCGGTCTTATCTTCATCAGCAATACCTCACCGGATACATATGACTCAAATATGGGTTATGTTGGGCCTAACCCTCTATATCTTCCTGATTATACAGATGATGATCTTGGTCAAATCTTAATGAGACACCAAGCAAACAAAAAGCTTTATACCTCTTTTCTCAGTGTTGTACTAAGGCCTTTCAGTAGAATTACCAGACGAGTGGATGAATTGTCTAGTGCATTTTCACTATTATTCAGAAAATATTGTGAACCTATTACTGATCTTGGGGTTGTTCCCAATGAAGAAATGAAGAGAAAGTTGTTTAGTCAATTTCAGCCCTATATTGCTCCTGCACTGAATGAGATATTTAAGGTTTCAACTCAGCCTTCCACTGAAGTTGAAGCTAGGGAGAGAAAGCCAACGGTTAGCACAAGGAAATCCCGAGGTTATGAAGTTGTTGACCAGTTAGATTTTTACATGTCTACCTCTGCCAAGTATCTTCTAATTTCAGCATTCCTTGCTTCAAGAAATCCAGCTACCCTTGATGCATCATTGTTCGATTCTACTGGAGGTTCTAATAATCGAAAACGAAAGAGGAAGGTCTCTGAGAAGTCAATGGAACAGAAGGAAACTGCAGAACAGGAATTACTCATGAAGGGCCCCGGAACATTCCCAATGGAGAGGTTACTAGCCATATTCCAGTGTATTACATCTGTGGCGGAAGATCCACTTGATGATGAGGAACAAGCGCAGGATGGGTTAGGGATTCAAGATGGGCATTGTGGGCTCATGTCTGATGTCCTCTTACAATTGACTAGTCTCTGTGGTGCAAATTTTATTGTCAAAGGAGGAAGCTGCCCACTCGAGGGCTCAACTCGCTATCGTTCTACAGTTAGTGAAGATATGGCCCTGCAGGTAGCAAGGAGCATTAAGTTTCCTCTCTCAAAGTACTTGTACAGAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000808 GO:0003674 GO:0003676 GO:0003677 GO:0003688 GO:0003690 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005664 GO:0005694 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006270 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009743 GO:0009744 GO:0009987 GO:0010033 GO:0031974 GO:0031981 GO:0032991 GO:0034285 GO:0034641 GO:0034645 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0050896 GO:0070013 GO:0071704 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901576 GO:1901700 GO:1990837

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 map04110 map04111 map04113

Pfam Domains

Protein Families

AAA_16 ORC5_C

Protein Analysis

998

Amino Acids

112.59

Weight (kDa)

5.67

Isoelectric Point (pI)

49.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	14 - 61	1.5e-14	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	17 - 76	2.9e-14	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	67 - 112	3.3e-17	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	71 - 114	3.3e-09	Myb-like DNA-binding domain
AWS	PF17907	383 - 415	2.3e-09	AWS domain
AAA_16	PF13191	520 - 681	3e-10	AAA ATPase domain
ORC5_lid	PF21639	733 - 786	2.7e-11	ORC5, lid domain
ORC5_C	PF14630	837 - 995	1.2e-38	Origin recognition complex (ORC) subunit 5 C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002753)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29910 AT4G29910
fragaria_vesca	FvH4_7g14490 FvH4_7g14490 FvH4_7g14490 FvH4_7g14490 FvH4_7g14490
malus_domestica	MD01G1065000.v1.1 MD07G1135400.v1.1
prunus_persica	Prupe.2G172000_v2.0.a1 Prupe.2G172000_v2.0.a1 Prupe.2G172000_v2.0.a1
pyrus_communis	pycom01g09430 pycom07g13260
rosa_chinensis	RchiOBHm_Chr1g0355901 RchiOBHm_Chr2g0161261
rosa_laevigata	RLG00000021273 RLG00000028117
rosa_multiflora	Rmu_sc0004705.1_g000017 Rmu_sc0009869.1_g000009
rosa_roxburghii	Rroxscaffold_2G00088970 Rroxscaffold_2G00110130 Rroxscaffold_4G00299930
rosa_rugosa	Rorug01G0248300 Rorug01G0248400 Rorug01G0248500 Rorug02G0487300 Rorug02G0487400 Rorug02G0487500
rosa_samantha	Rh1AG261600 Rh1BG229700 Rh1CG244700 Rh1DG257700 Rh2AG554200 Rh2BG567600 Rh2CG538100 Rh2DG577000
rosa_wichuraiana	Rw1G023100 Rw2G045850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1734
Acc36I	ACCTGC	1 cut(s)	2935
AccB1I	GGYRCC	1 cut(s)	111
AccI	GTMKAC	1 cut(s)	2513
AccII	CGCG	1 cut(s)	1857
AccIII	TCCGGA	1 cut(s)	55
AciI	CCGC	7 cut(s)	278, 575, 591, 950, 1305, 1607, 2750
AclI	AACGTT	1 cut(s)	343
AclWI	GGATC	7 cut(s)	435, 931, 944, 1155, 1293, 1419, 2750
AcoI	YGGCCR	1 cut(s)	1437
AcsI	RAATTY	7 cut(s)	486, 841, 1073, 1246, 1404, 1846, 2863
AcuI	CTGAAG	4 cut(s)	272, 609, 955, 2445
AcyI	GRCGYC	2 cut(s)	112, 368
AfaI	GTAC	7 cut(s)	46, 414, 1018, 1052, 2220, 2981, 2987
AfeI	AGCGCT	1 cut(s)	1820
AfiI	CCNNNNNNNGG	3 cut(s)	2051, 2896, 2897
AflIII	ACRYGT	2 cut(s)	826, 2508
AhdI	GACNNNNNGTC	1 cut(s)	997
AhlI	ACTAGT	1 cut(s)	2846
AjnI	CCWGG	3 cut(s)	786, 1207, 1561
AjuI	GAANNNNNNNTTGG	2 cut(s)	1348, 1380
Alw21I	GWGCWC	1 cut(s)	1596
Alw26I	GTCTC	7 cut(s)	556, 940, 984, 998, 2167, 2638, 2855
Alw44I	GTGCAC	1 cut(s)	1592
AlwI	GGATC	7 cut(s)	435, 931, 944, 1155, 1293, 1419, 2750
Ama87I	CYCGRG	3 cut(s)	2045, 2472, 2894
Aor13HI	TCCGGA	1 cut(s)	55
Aor51HI	AGCGCT	1 cut(s)	1820
AoxI	GGCC	7 cut(s)	185, 1437, 1829, 2114, 2225, 2689, 2937
ApaI	GGGCCC	1 cut(s)	2693
ApaLI	GTGCAC	1 cut(s)	1592
ApeKI	GCWGC	5 cut(s)	99, 629, 1486, 1719, 2886
ApoI	RAATTY	7 cut(s)	486, 841, 1073, 1246, 1404, 1846, 2863
Asp700I	GAANNNNTTC	2 cut(s)	681, 1073
AspLEI	GCGC	3 cut(s)	114, 1821, 2782
AspS9I	GGNCC	5 cut(s)	1604, 2114, 2689, 2690, 2938
AsuC2I	CCSGG	1 cut(s)	2694
AsuHPI	GGTGA	3 cut(s)	16, 1772, 2070
AvaI	CYCGRG	3 cut(s)	2045, 2472, 2894
AvaII	GGWCC	1 cut(s)	1604
AxyI	CCTNAGG	1 cut(s)	1887
BaeGI	GKGCMC	2 cut(s)	1596, 2693
BaeI	ACNNNNGTAYC	2 cut(s)	561, 594
BalI	TGGCCA	1 cut(s)	1439
BamHI	GGATCC	1 cut(s)	936
BanI	GGYRCC	1 cut(s)	111
BanII	GRGCYC	4 cut(s)	1047, 2693, 2820, 2903
BarI	GAAGNNNNNNTAC	2 cut(s)	396, 428
BbsI	GAAGAC	1 cut(s)	899
Bbv12I	GWGCWC	1 cut(s)	1596
BbvCI	CCTCAGC	1 cut(s)	164
BbvI	GCAGC	5 cut(s)	86, 616, 1498, 1706, 2873
BccI	CCATC	6 cut(s)	544, 590, 724, 1840, 2780, 2798
BciT130I	CCWGG	3 cut(s)	788, 1209, 1563
BciVI	GTATCC	1 cut(s)	2077
BclI	TGATCA	2 cut(s)	394, 481
BcnI	CCSGG	1 cut(s)	2694
BcoDI	GTCTC	7 cut(s)	556, 940, 984, 998, 2167, 2638, 2855
BcuI	ACTAGT	1 cut(s)	2846
BfaI	CTAG	9 cut(s)	90, 872, 977, 1554, 1809, 2265, 2436, 2720, 2847
BfmI	CTRYAG	3 cut(s)	2664, 2919, 2942
BfoI	RGCGCY	2 cut(s)	115, 1822
BfuAI	ACCTGC	1 cut(s)	2935
BfuI	GTATCC	1 cut(s)	2077
BisI	GCNGC	7 cut(s)	100, 279, 591, 630, 1487, 1720, 2887
BlsI	GCNGC	7 cut(s)	101, 280, 592, 631, 1488, 1721, 2888
BmcAI	AGTACT	1 cut(s)	2981
Bme1390I	CCNGG	4 cut(s)	788, 1209, 1563, 2694
Bme18I	GGWCC	1 cut(s)	1604
BmeRI	GACNNNNNGTC	1 cut(s)	997
BmeT110I	CYCGRG	3 cut(s)	2045, 2472, 2894
BmgT120I	GGNCC	5 cut(s)	1604, 2114, 2689, 2690, 2938
BmiI	GGNNCC	6 cut(s)	113, 735, 938, 1605, 2691, 2692
BmrFI	CCNGG	4 cut(s)	788, 1209, 1563, 2694
BmrI	ACTGGG	1 cut(s)	1042
BmsI	GCATC	6 cut(s)	907, 1351, 1462, 1699, 2566, 2588
BmuI	ACTGGG	1 cut(s)	1042
BoxI	GACNNNNGTC	2 cut(s)	1010, 1897
BpiI	GAAGAC	1 cut(s)	899
BpmI	CTGGAG	1 cut(s)	2619
Bpu10I	CCTNAGC	1 cut(s)	164
BpuEI	CTTGAG	3 cut(s)	202, 320, 1699
BpuMI	CCSGG	1 cut(s)	2694
BsaBI	GATNNNNATC	2 cut(s)	396, 2151
BsaHI	GRCGYC	2 cut(s)	112, 368
BsaI	GGTCTC	3 cut(s)	556, 940, 2638
BsaJI	CCNNGG	5 cut(s)	633, 1863, 2046, 2473, 2692
BsaWI	WCCGGW	2 cut(s)	55, 2080
BsaXI	ACNNNNNCTCC	4 cut(s)	37, 67, 725, 755
Bsc4I	CCNNNNNNNGG	3 cut(s)	2051, 2896, 2897
Bse1I	ACTGG	8 cut(s)	326, 715, 1048, 1660, 1665, 2494, 2602, 2731
Bse21I	CCTNAGG	1 cut(s)	1887
Bse3DI	GCAATG	1 cut(s)	1205
Bse8I	GATNNNNATC	2 cut(s)	396, 2151
BseAI	TCCGGA	1 cut(s)	55
BseBI	CCWGG	3 cut(s)	788, 1209, 1563
BseDI	CCNNGG	5 cut(s)	633, 1863, 2046, 2473, 2692
BseGI	GGATG	2 cut(s)	2260, 2791
BseJI	GATNNNNATC	2 cut(s)	396, 2151
BseLI	CCNNNNNNNGG	3 cut(s)	2051, 2896, 2897
BseMI	GCAATG	1 cut(s)	1205
BseMII	CTCAG	7 cut(s)	155, 387, 729, 1901, 2224, 2426, 2628
BseNI	ACTGG	8 cut(s)	326, 715, 1048, 1660, 1665, 2494, 2602, 2731
BseRI	GAGGAG	2 cut(s)	1121, 1124
BseSI	GKGCMC	2 cut(s)	1596, 2693
BseXI	GCAGC	5 cut(s)	86, 616, 1498, 1706, 2873
BseYI	CCCAGC	2 cut(s)	131, 253
BsgI	GTGCAG	1 cut(s)	2367
Bsh1236I	CGCG	1 cut(s)	1857
BshFI	GGCC	7 cut(s)	187, 1439, 1831, 2116, 2227, 2691, 2939
BshNI	GGYRCC	1 cut(s)	111
BsiHKAI	GWGCWC	1 cut(s)	1596
BsiHKCI	CYCGRG	3 cut(s)	2045, 2472, 2894
BsiSI	CCGG	3 cut(s)	56, 2081, 2694
BslFI	GGGAC	2 cut(s)	1905, 2000
BslI	CCNNNNNNNGG	3 cut(s)	2051, 2896, 2897
BsmAI	GTCTC	7 cut(s)	556, 940, 984, 998, 2167, 2638, 2855
BsmFI	GGGAC	2 cut(s)	1905, 2000
BsmI	GAATGC	2 cut(s)	1133, 2543
BsnI	GGCC	7 cut(s)	187, 1439, 1831, 2116, 2227, 2691, 2939
Bso31I	GGTCTC	3 cut(s)	556, 940, 2638
BsoBI	CYCGRG	3 cut(s)	2045, 2472, 2894
Bsp120I	GGGCCC	1 cut(s)	2689
Bsp1286I	GDGCHC	5 cut(s)	1047, 1596, 2693, 2820, 2903
Bsp13I	TCCGGA	1 cut(s)	55
Bsp1407I	TGTACA	1 cut(s)	2985
Bsp19I	CCATGG	1 cut(s)	633
Bsp68I	TCGCGA	1 cut(s)	1857
BspACI	CCGC	7 cut(s)	278, 575, 591, 950, 1305, 1607, 2750
BspANI	GGCC	7 cut(s)	187, 1439, 1831, 2116, 2227, 2691, 2939
BspCNI	CTCAG	7 cut(s)	156, 386, 730, 1900, 2223, 2425, 2629
BspEI	TCCGGA	1 cut(s)	55
BspFNI	CGCG	1 cut(s)	1857
BspHI	TCATGA	2 cut(s)	400, 2682
BspLI	GGNNCC	6 cut(s)	113, 735, 938, 1605, 2691, 2692
BspMAI	CTGCAG	2 cut(s)	2668, 2946
BspMI	ACCTGC	1 cut(s)	2935
BspPI	GGATC	7 cut(s)	435, 931, 944, 1155, 1293, 1419, 2750
BspQI	GCTCTTC	1 cut(s)	1654
BspT107I	GGYRCC	1 cut(s)	111
BspTNI	GGTCTC	3 cut(s)	556, 940, 2638
BsrDI	GCAATG	1 cut(s)	1205
BsrGI	TGTACA	1 cut(s)	2985
BsrI	ACTGG	8 cut(s)	326, 715, 1048, 1660, 1665, 2494, 2602, 2731
BssECI	CCNNGG	5 cut(s)	633, 1863, 2046, 2473, 2692
BssNI	GRCGYC	2 cut(s)	112, 368
BssT1I	CCWWGG	2 cut(s)	633, 1863
Bst2UI	CCWGG	3 cut(s)	788, 1209, 1563
Bst4CI	ACNGT	6 cut(s)	478, 522, 884, 2002, 2455, 2923
Bst6I	CTCTTC	4 cut(s)	146, 216, 1654, 2336
BstACI	GRCGYC	2 cut(s)	112, 368
BstAPI	GCANNNNNTGC	2 cut(s)	1200, 2549
BstAUI	TGTACA	1 cut(s)	2985
BstC8I	GCNNGC	2 cut(s)	161, 1196
BstDEI	CTNAG	8 cut(s)	164, 373, 738, 1887, 2210, 2222, 2412, 2637
BstDSI	CCRYGG	1 cut(s)	633
BstF5I	GGATG	2 cut(s)	2260, 2791
BstFNI	CGCG	1 cut(s)	1857
BstH2I	RGCGCY	2 cut(s)	115, 1822
BstHHI	GCGC	3 cut(s)	114, 1821, 2782
BstMAI	GTCTC	7 cut(s)	556, 940, 984, 998, 2167, 2638, 2855
BstNI	CCWGG	3 cut(s)	788, 1209, 1563
BstNSI	RCATGY	3 cut(s)	830, 1164, 2512
BstPAI	GACNNNNGTC	2 cut(s)	1010, 1897
BstSCI	CCNGG	4 cut(s)	786, 1207, 1561, 2692
BstSFI	CTRYAG	3 cut(s)	2664, 2919, 2942
BstSLI	GKGCMC	2 cut(s)	1596, 2693
BstUI	CGCG	1 cut(s)	1857
BstV1I	GCAGC	5 cut(s)	86, 616, 1498, 1706, 2873
BstV2I	GAAGAC	1 cut(s)	899
BstX2I	RGATCY	4 cut(s)	936, 1147, 1298, 2755
BstXI	CCANNNNNNTGG	2 cut(s)	522, 2251
BstYI	RGATCY	4 cut(s)	936, 1147, 1298, 2755
Bsu36I	CCTNAGG	1 cut(s)	1887
BsuI	GTATCC	1 cut(s)	2077
BsuRI	GGCC	7 cut(s)	187, 1439, 1831, 2116, 2227, 2691, 2939
BtgI	CCRYGG	1 cut(s)	633
BtsCI	GGATG	2 cut(s)	2260, 2791
BtsIMutI	CAGTG	8 cut(s)	319, 769, 889, 1653, 2218, 2384, 2421, 2738
BtuMI	TCGCGA	1 cut(s)	1857
BveI	ACCTGC	1 cut(s)	2935
Cac8I	GCNNGC	2 cut(s)	161, 1196
CciI	TCATGA	2 cut(s)	400, 2682
CfoI	GCGC	3 cut(s)	114, 1821, 2782
Cfr13I	GGNCC	5 cut(s)	1604, 2114, 2689, 2690, 2938
CseI	GACGC	1 cut(s)	357
CsiI	ACCWGGT	1 cut(s)	786
Csp6I	GTAC	7 cut(s)	45, 413, 1017, 1051, 2219, 2980, 2986
CspCI	CAANNNNNGTGG	2 cut(s)	2410, 2445
CviQI	GTAC	7 cut(s)	45, 413, 1017, 1051, 2219, 2980, 2986
DdeI	CTNAG	8 cut(s)	164, 373, 738, 1887, 2210, 2222, 2412, 2637
DinI	GGCGCC	1 cut(s)	113
DriI	GACNNNNNGTC	1 cut(s)	997
EaeI	YGGCCR	1 cut(s)	1437
Eam1104I	CTCTTC	4 cut(s)	146, 216, 1654, 2336
Eam1105I	GACNNNNNGTC	1 cut(s)	997
EarI	CTCTTC	4 cut(s)	146, 216, 1654, 2336
EciI	GGCGGA	1 cut(s)	2765
Eco130I	CCWWGG	2 cut(s)	633, 1863
Eco147I	AGGCCT	1 cut(s)	2227
Eco24I	GRGCYC	4 cut(s)	1047, 2693, 2820, 2903
Eco31I	GGTCTC	3 cut(s)	556, 940, 2638
Eco47I	GGWCC	1 cut(s)	1604
Eco47III	AGCGCT	1 cut(s)	1820
Eco57I	CTGAAG	4 cut(s)	272, 609, 955, 2445
Eco81I	CCTNAGG	1 cut(s)	1887
Eco88I	CYCGRG	3 cut(s)	2045, 2472, 2894
EcoO109I	RGGNCCY	2 cut(s)	2689, 2690
EcoRI	GAATTC	2 cut(s)	1073, 1246
EcoRII	CCWGG	3 cut(s)	786, 1207, 1561
EcoT14I	CCWWGG	2 cut(s)	633, 1863
EcoT22I	ATGCAT	1 cut(s)	2581
EcoT38I	GRGCYC	4 cut(s)	1047, 2693, 2820, 2903
EgeI	GGCGCC	1 cut(s)	113
EheI	GGCGCC	1 cut(s)	113
ErhI	CCWWGG	2 cut(s)	633, 1863
FalI	AAGNNNNNCTT	2 cut(s)	2745, 2777
FaqI	GGGAC	2 cut(s)	1905, 2000
FauNDI	CATATG	2 cut(s)	1121, 2089
FbaI	TGATCA	2 cut(s)	394, 481
FblI	GTMKAC	1 cut(s)	2513
Fnu4HI	GCNGC	7 cut(s)	100, 279, 591, 630, 1487, 1720, 2887
FokI	GGATG	2 cut(s)	2267, 2798
FriOI	GRGCYC	4 cut(s)	1047, 2693, 2820, 2903
Fsp4HI	GCNGC	7 cut(s)	100, 279, 591, 630, 1487, 1720, 2887
FspBI	CTAG	9 cut(s)	90, 872, 977, 1554, 1809, 2265, 2436, 2720, 2847
GlaI	GCGC	3 cut(s)	113, 1820, 2781
GluI	GCNGC	7 cut(s)	100, 279, 591, 630, 1487, 1720, 2887
GsaI	CCCAGC	2 cut(s)	135, 257
GsuI	CTGGAG	1 cut(s)	2619
HaeII	RGCGCY	2 cut(s)	115, 1822
HaeIII	GGCC	7 cut(s)	187, 1439, 1831, 2116, 2227, 2691, 2939
HapII	CCGG	3 cut(s)	56, 2081, 2694
HgaI	GACGC	1 cut(s)	357
HhaI	GCGC	3 cut(s)	114, 1821, 2782
Hin1I	GRCGYC	2 cut(s)	112, 368
Hin6I	GCGC	3 cut(s)	112, 1819, 2780
HinP1I	GCGC	3 cut(s)	112, 1819, 2780
HincII	GTYRAC	2 cut(s)	1188, 2491
HindII	GTYRAC	2 cut(s)	1188, 2491
HindIII	AAGCTT	2 cut(s)	958, 2192
HpaII	CCGG	3 cut(s)	56, 2081, 2694
HphI	GGTGA	3 cut(s)	16, 1772, 2070
Hpy166II	GTNNAC	8 cut(s)	613, 1053, 1188, 1594, 1711, 2300, 2491, 2514
Hpy8I	GTNNAC	8 cut(s)	613, 1053, 1188, 1594, 1711, 2300, 2491, 2514
HpyAV	CCTTC	8 cut(s)	979, 1080, 1514, 1787, 2427, 2623, 2650, 2680
HpyCH4III	ACNGT	6 cut(s)	478, 522, 884, 2002, 2455, 2923
HpyCH4IV	ACGT	1 cut(s)	343
HpyF3I	CTNAG	8 cut(s)	164, 373, 738, 1887, 2210, 2222, 2412, 2637
HpySE526I	ACGT	1 cut(s)	343
Hsp92I	GRCGYC	2 cut(s)	112, 368
HspAI	GCGC	3 cut(s)	112, 1819, 2780
KasI	GGCGCC	1 cut(s)	111
Kpn2I	TCCGGA	1 cut(s)	55
Ksp22I	TGATCA	2 cut(s)	394, 481
LguI	GCTCTTC	1 cut(s)	1654
LmnI	GCTCC	4 cut(s)	733, 1969, 2383, 2955
Lsp1109I	GCAGC	5 cut(s)	86, 616, 1498, 1706, 2873
LweI	GCATC	6 cut(s)	907, 1351, 1462, 1699, 2566, 2588
MabI	ACCWGGT	1 cut(s)	786
MaeI	CTAG	9 cut(s)	90, 872, 977, 1554, 1809, 2265, 2436, 2720, 2847
MaeII	ACGT	1 cut(s)	343
MaeIII	GTNAC	3 cut(s)	85, 2004, 2715
MfeI	CAATTG	1 cut(s)	2840
MflI	RGATCY	4 cut(s)	936, 1147, 1298, 2755
MhlI	GDGCHC	5 cut(s)	1047, 1596, 2693, 2820, 2903
MlsI	TGGCCA	1 cut(s)	1439
MluNI	TGGCCA	1 cut(s)	1439
Mly113I	GGCGCC	1 cut(s)	112
MlyI	GAGTC	4 cut(s)	46, 1007, 1535, 2087
MmeI	TCCRAC	1 cut(s)	756
Mox20I	TGGCCA	1 cut(s)	1439
Mph1103I	ATGCAT	1 cut(s)	2581
MroI	TCCGGA	1 cut(s)	55
MroXI	GAANNNNTTC	2 cut(s)	681, 1073
MscI	TGGCCA	1 cut(s)	1439
MseI	TTAA	9 cut(s)	312, 347, 1260, 1695, 1763, 2019, 2168, 2400, 2961
MslI	CAYNNNNRTG	1 cut(s)	1356
Msp20I	TGGCCA	1 cut(s)	1439
MspA1I	CMGCKG	1 cut(s)	629
MspI	CCGG	3 cut(s)	56, 2081, 2694
MspR9I	CCNGG	4 cut(s)	788, 1209, 1563, 2694
MunI	CAATTG	1 cut(s)	2840
Mva1269I	GAATGC	2 cut(s)	1133, 2543
MvaI	CCWGG	3 cut(s)	788, 1209, 1563
MvnI	CGCG	1 cut(s)	1857
NarI	GGCGCC	1 cut(s)	112
NciI	CCSGG	1 cut(s)	2694
NcoI	CCATGG	1 cut(s)	633
NdeI	CATATG	2 cut(s)	1121, 2089
NlaIV	GGNNCC	6 cut(s)	113, 735, 938, 1605, 2691, 2692
NruI	TCGCGA	1 cut(s)	1857
NsiI	ATGCAT	1 cut(s)	2581
NspI	RCATGY	3 cut(s)	830, 1164, 2512
PaeR7I	CTCGAG	1 cut(s)	2894
PagI	TCATGA	2 cut(s)	400, 2682
PceI	AGGCCT	1 cut(s)	2227
PciI	ACATGT	2 cut(s)	826, 2508
PciSI	GCTCTTC	1 cut(s)	1654
PctI	GAATGC	2 cut(s)	1133, 2543
PdmI	GAANNNNTTC	2 cut(s)	681, 1073
PfeI	GAWTC	7 cut(s)	316, 1114, 1240, 1743, 1754, 2591, 2797
PfoI	TCCNGGA	1 cut(s)	1207
PkrI	GCNGC	7 cut(s)	101, 280, 592, 631, 1488, 1721, 2888
PleI	GAGTC	4 cut(s)	46, 1006, 1534, 2087
PluTI	GGCGCC	1 cut(s)	115
PpsI	GAGTC	4 cut(s)	46, 1006, 1534, 2087
PscI	ACATGT	2 cut(s)	826, 2508
PshAI	GACNNNNGTC	2 cut(s)	1010, 1897
PsiI	TTATAA	1 cut(s)	1734
Psp1406I	AACGTT	1 cut(s)	343
Psp6I	CCWGG	3 cut(s)	786, 1207, 1561
PspFI	CCCAGC	2 cut(s)	131, 253
PspGI	CCWGG	3 cut(s)	786, 1207, 1561
PspN4I	GGNNCC	6 cut(s)	113, 735, 938, 1605, 2691, 2692
PspOMI	GGGCCC	1 cut(s)	2689
PspPI	GGNCC	5 cut(s)	1604, 2114, 2689, 2690, 2938
PspXI	VCTCGAGB	1 cut(s)	2894
PsrI	GAACNNNNNNTAC	2 cut(s)	2661, 2693
PstI	CTGCAG	2 cut(s)	2668, 2946
PsuI	RGATCY	4 cut(s)	936, 1147, 1298, 2755
PvuII	CAGCTG	1 cut(s)	629
RruI	TCGCGA	1 cut(s)	1857
RsaI	GTAC	7 cut(s)	46, 414, 1018, 1052, 2220, 2981, 2987
RsaNI	GTAC	7 cut(s)	45, 413, 1017, 1051, 2219, 2980, 2986
RseI	CAYNNNNRTG	1 cut(s)	1356
SapI	GCTCTTC	1 cut(s)	1654
SaqAI	TTAA	9 cut(s)	312, 347, 1260, 1695, 1763, 2019, 2168, 2400, 2961
SatI	GCNGC	7 cut(s)	100, 279, 591, 630, 1487, 1720, 2887
Sau96I	GGNCC	5 cut(s)	1604, 2114, 2689, 2690, 2938
SbfI	CCTGCAGG	1 cut(s)	2946
ScaI	AGTACT	1 cut(s)	2981
SchI	GAGTC	4 cut(s)	46, 1007, 1535, 2087
ScrFI	CCNGG	4 cut(s)	788, 1209, 1563, 2694
SdaI	CCTGCAGG	1 cut(s)	2946
SduI	GDGCHC	5 cut(s)	1047, 1596, 2693, 2820, 2903
SexAI	ACCWGGT	1 cut(s)	786
SfaNI	GCATC	6 cut(s)	907, 1351, 1462, 1699, 2566, 2588
SfcI	CTRYAG	3 cut(s)	2664, 2919, 2942
SfoI	GGCGCC	1 cut(s)	113
Sfr274I	CTCGAG	1 cut(s)	2894
SinI	GGWCC	1 cut(s)	1604
SlaI	CTCGAG	1 cut(s)	2894
SmiMI	CAYNNNNRTG	1 cut(s)	1356
SmlI	CTYRAG	4 cut(s)	181, 335, 1714, 2894
SmoI	CTYRAG	4 cut(s)	181, 335, 1714, 2894
SpeI	ACTAGT	1 cut(s)	2846
Sse8387I	CCTGCAGG	1 cut(s)	2946
SseBI	AGGCCT	1 cut(s)	2227
SsiI	CCGC	7 cut(s)	278, 575, 591, 950, 1305, 1607, 2750
SspDI	GGCGCC	1 cut(s)	111
SspI	AATATT	1 cut(s)	2294
SspMI	CTAG	9 cut(s)	90, 872, 977, 1554, 1809, 2265, 2436, 2720, 2847
StuI	AGGCCT	1 cut(s)	2227
StyD4I	CCNGG	4 cut(s)	786, 1207, 1561, 2692
StyI	CCWWGG	2 cut(s)	633, 1863
TaaI	ACNGT	6 cut(s)	478, 522, 884, 2002, 2455, 2923
TaiI	ACGT	1 cut(s)	346
TaqI	TCGA	6 cut(s)	542, 1539, 1741, 2589, 2614, 2895
TaqII	GACCGA	1 cut(s)	2042
TatI	WGTACW	4 cut(s)	1050, 2218, 2979, 2985
TauI	GCSGC	2 cut(s)	281, 593
TfiI	GAWTC	7 cut(s)	316, 1114, 1240, 1743, 1754, 2591, 2797
Tru1I	TTAA	9 cut(s)	312, 347, 1260, 1695, 1763, 2019, 2168, 2400, 2961
Tru9I	TTAA	9 cut(s)	312, 347, 1260, 1695, 1763, 2019, 2168, 2400, 2961
TscAI	CASTG	8 cut(s)	326, 769, 889, 1660, 2218, 2391, 2428, 2738
TseI	GCWGC	5 cut(s)	99, 629, 1486, 1719, 2886
TspGWI	ACGGA	2 cut(s)	767, 1420
TspRI	CASTG	8 cut(s)	326, 769, 889, 1660, 2218, 2391, 2428, 2738
VneI	GTGCAC	1 cut(s)	1592
VpaK11BI	GGWCC	1 cut(s)	1604
XapI	RAATTY	7 cut(s)	486, 841, 1073, 1246, 1404, 1846, 2863
XceI	RCATGY	3 cut(s)	830, 1164, 2512
XhoI	CTCGAG	1 cut(s)	2894
XmiI	GTMKAC	1 cut(s)	2513
XmnI	GAANNNNTTC	2 cut(s)	681, 1073
XspI	CTAG	9 cut(s)	90, 872, 977, 1554, 1809, 2265, 2436, 2720, 2847
ZrmI	AGTACT	1 cut(s)	2981
Zsp2I	ATGCAT	1 cut(s)	2581

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.