Rroxscaffold_2G00098940

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002939
Physical Location & Seq
Reverse (-)
20427368 .. 20429649
2282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00098940.1

Sequence Viewer

Length: 399 bp
ATGGAGAGCGAAGGAATTGAATCGAACAGAAAACGCATGAAGCCTGATAGTCGCGAGGAAGGAGACGGCGCCGTCTCAGACTCGGAGATGGCGCAGAGGGAAGAGGTCGAGATGAGTGTGGCTGGATCTGAAGAGGTGGAGCTTTCGATCAGTCAGATCCTTGGAAAGATTGACCGGTTCACTGAGATGATATCGGAGCTGCTTGATTCGGGGAAGGCGGTGTTCAAGAAGATCAGCGATGATTATGAGGAGCGATTGATTATGATTCACAAGGAGCAAATGGAGAAATGGCAGGAGGAGATCAGAGAGTTGCGCGCGATTGATGCTTCAAATGAGGAGGCTAGTGCTGTGCTGCATAATGCTACATATCTGCTTCAGAATCCTCATGTTGATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

15.06

Weight (kDa)

4.51

Isoelectric Point (pI)

59.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 71
AccB1I GGYRCC 1 cut(s) 68
AccII CGCG 3 cut(s) 54, 315, 317
AciI CCGC 1 cut(s) 218
AclWI GGATC 2 cut(s) 133, 151
AcuI CTGAAG 2 cut(s) 150, 359
AcyI GRCGYC 1 cut(s) 69
AgeI ACCGGT 1 cut(s) 174
AgsI TTSAA 3 cut(s) 20, 226, 330
AluBI AGCT 2 cut(s) 142, 199
AluI AGCT 2 cut(s) 142, 199
Alw26I GTCTC 2 cut(s) 57, 79
AlwI GGATC 2 cut(s) 133, 151
ApeKI GCWGC 2 cut(s) 199, 352
AsiGI ACCGGT 1 cut(s) 174
AspLEI GCGC 4 cut(s) 71, 94, 315, 317
BanI GGYRCC 1 cut(s) 68
BbvI GCAGC 2 cut(s) 186, 339
BccI CCATC 1 cut(s) 82
BceAI ACGGC 2 cut(s) 56, 82
BcoDI GTCTC 2 cut(s) 57, 79
BfaI CTAG 1 cut(s) 342
BfoI RGCGCY 1 cut(s) 72
BisI GCNGC 2 cut(s) 200, 353
BlsI GCNGC 2 cut(s) 201, 354
BmiI GGNNCC 1 cut(s) 70
BmsI GCATC 1 cut(s) 313
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 160
BsaWI WCCGGW 1 cut(s) 174
Bse118I RCCGGY 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 160
BseMII CTCAG 2 cut(s) 90, 174
BsePI GCGCGC 1 cut(s) 313
BseRI GAGGAG 3 cut(s) 263, 311, 350
BseXI GCAGC 2 cut(s) 186, 339
Bsh1236I CGCG 3 cut(s) 54, 315, 317
BshNI GGYRCC 1 cut(s) 68
BshTI ACCGGT 1 cut(s) 174
BsiSI CCGG 1 cut(s) 175
BsmAI GTCTC 2 cut(s) 57, 79
BsmBI CGTCTC 2 cut(s) 57, 79
Bsp143I GATC 5 cut(s) 125, 147, 156, 231, 300
Bsp68I TCGCGA 1 cut(s) 54
BspACI CCGC 1 cut(s) 218
BspCNI CTCAG 2 cut(s) 89, 175
BspFNI CGCG 3 cut(s) 54, 315, 317
BspLI GGNNCC 1 cut(s) 70
BspPI GGATC 2 cut(s) 133, 151
BspT107I GGYRCC 1 cut(s) 68
BsrFI RCCGGY 1 cut(s) 174
BssAI RCCGGY 1 cut(s) 174
BssECI CCNNGG 1 cut(s) 160
BssHII GCGCGC 1 cut(s) 313
BssMI GATC 5 cut(s) 125, 147, 156, 231, 300
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 160
Bst6I CTCTTC 2 cut(s) 96, 126
BstACI GRCGYC 1 cut(s) 69
BstC8I GCNNGC 1 cut(s) 315
BstDEI CTNAG 2 cut(s) 76, 183
BstFNI CGCG 3 cut(s) 54, 315, 317
BstH2I RGCGCY 1 cut(s) 72
BstHHI GCGC 4 cut(s) 71, 94, 315, 317
BstKTI GATC 5 cut(s) 128, 150, 159, 234, 303
BstMAI GTCTC 2 cut(s) 57, 79
BstMBI GATC 5 cut(s) 125, 147, 156, 231, 300
BstMWI GCNNNNNNNGC 1 cut(s) 323
BstUI CGCG 3 cut(s) 54, 315, 317
BstV1I GCAGC 2 cut(s) 186, 339
BstX2I RGATCY 2 cut(s) 125, 156
BstYI RGATCY 2 cut(s) 125, 156
BtgZI GCGATG 1 cut(s) 252
BtsIMutI CAGTG 1 cut(s) 180
BtuMI TCGCGA 1 cut(s) 54
Cac8I GCNNGC 1 cut(s) 315
CfoI GCGC 4 cut(s) 71, 94, 315, 317
Cfr10I RCCGGY 1 cut(s) 174
CspAI ACCGGT 1 cut(s) 174
CviAII CATG 2 cut(s) 37, 386
CviJI RGCY 5 cut(s) 43, 122, 142, 199, 341
CviKI_1 RGCY 5 cut(s) 43, 122, 142, 199, 341
DdeI CTNAG 2 cut(s) 76, 183
DinI GGCGCC 1 cut(s) 70
DpnI GATC 5 cut(s) 127, 149, 158, 233, 302
DpnII GATC 5 cut(s) 125, 147, 156, 231, 300
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
Eam1104I CTCTTC 2 cut(s) 96, 126
EarI CTCTTC 2 cut(s) 96, 126
Eco130I CCWWGG 1 cut(s) 160
Eco32I GATATC 1 cut(s) 192
Eco57I CTGAAG 2 cut(s) 150, 359
EcoRV GATATC 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 160
EgeI GGCGCC 1 cut(s) 70
EheI GGCGCC 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 160
Esp3I CGTCTC 2 cut(s) 57, 79
FaeI CATG 2 cut(s) 40, 389
FaiI YATR 6 cut(s) 38, 246, 263, 357, 367, 387
FatI CATG 2 cut(s) 36, 385
Fnu4HI GCNGC 2 cut(s) 200, 353
Fsp4HI GCNGC 2 cut(s) 200, 353
FspBI CTAG 1 cut(s) 342
GlaI GCGC 4 cut(s) 70, 93, 314, 316
GluI GCNGC 2 cut(s) 200, 353
HaeII RGCGCY 1 cut(s) 72
HapII CCGG 1 cut(s) 175
HhaI GCGC 4 cut(s) 71, 94, 315, 317
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 2 cut(s) 40, 389
Hin6I GCGC 4 cut(s) 69, 92, 313, 315
HinP1I GCGC 4 cut(s) 69, 92, 313, 315
HinfI GANTC 6 cut(s) 20, 80, 206, 265, 379, 392
HpaII CCGG 1 cut(s) 175
Hpy166II GTNNAC 1 cut(s) 180
Hpy188I TCNGA 7 cut(s) 79, 85, 130, 156, 196, 305, 378
Hpy188III TCNNGA 4 cut(s) 53, 109, 226, 396
Hpy8I GTNNAC 1 cut(s) 180
HpyAV CCTTC 3 cut(s) 5, 53, 208
HpyCH4V TGCA 1 cut(s) 355
HpyF10VI GCNNNNNNNGC 1 cut(s) 323
HpyF3I CTNAG 2 cut(s) 76, 183
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 2 cut(s) 40, 389
HspAI GCGC 4 cut(s) 69, 92, 313, 315
KasI GGCGCC 1 cut(s) 68
Kzo9I GATC 5 cut(s) 125, 147, 156, 231, 300
LmnI GCTCC 4 cut(s) 139, 196, 250, 274
LpnPI CCDG 4 cut(s) 57, 108, 188, 278
Lsp1109I GCAGC 2 cut(s) 186, 339
LweI GCATC 1 cut(s) 313
MaeI CTAG 1 cut(s) 342
MalI GATC 5 cut(s) 127, 149, 158, 233, 302
MboI GATC 5 cut(s) 125, 147, 156, 231, 300
MboII GAAGA 3 cut(s) 113, 143, 241
MflI RGATCY 2 cut(s) 125, 156
MluCI AATT 1 cut(s) 15
Mly113I GGCGCC 1 cut(s) 69
MlyI GAGTC 1 cut(s) 74
MnlI CCTC 9 cut(s) 49, 90, 97, 127, 241, 289, 328, 331, 393
MslI CAYNNNNRTG 1 cut(s) 185
MspI CCGG 1 cut(s) 175
MvnI CGCG 3 cut(s) 54, 315, 317
MwoI GCNNNNNNNGC 1 cut(s) 323
NarI GGCGCC 1 cut(s) 69
NdeII GATC 5 cut(s) 125, 147, 156, 231, 300
NlaIII CATG 2 cut(s) 40, 389
NlaIV GGNNCC 1 cut(s) 70
NruI TCGCGA 1 cut(s) 54
PauI GCGCGC 1 cut(s) 313
PfeI GAWTC 5 cut(s) 20, 206, 265, 379, 392
PinAI ACCGGT 1 cut(s) 174
PkrI GCNGC 2 cut(s) 201, 354
PleI GAGTC 1 cut(s) 74
PluTI GGCGCC 1 cut(s) 72
PpsI GAGTC 1 cut(s) 74
PspN4I GGNNCC 1 cut(s) 70
PsuI RGATCY 2 cut(s) 125, 156
PteI GCGCGC 1 cut(s) 313
RruI TCGCGA 1 cut(s) 54
RseI CAYNNNNRTG 1 cut(s) 185
SatI GCNGC 2 cut(s) 200, 353
Sau3AI GATC 5 cut(s) 125, 147, 156, 231, 300
SchI GAGTC 1 cut(s) 74
SetI ASST 4 cut(s) 108, 138, 144, 201
SfaNI GCATC 1 cut(s) 313
SfoI GGCGCC 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 1 cut(s) 15
SsiI CCGC 1 cut(s) 218
SspDI GGCGCC 1 cut(s) 68
SspMI CTAG 1 cut(s) 342
StyI CCWWGG 1 cut(s) 160
TaqI TCGA 3 cut(s) 23, 108, 146
TasI AATT 1 cut(s) 15
TfiI GAWTC 5 cut(s) 20, 206, 265, 379, 392
TscAI CASTG 1 cut(s) 187
TseI GCWGC 2 cut(s) 199, 352
TspDTI ATGAA 1 cut(s) 53
TspRI CASTG 1 cut(s) 187
XspI CTAG 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.