Rorug02G0405300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002939
Physical Location & Seq
Forward (+)
51745462 .. 51746503
1042 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0405300.1

Sequence Viewer

Length: 939 bp
ATGCATGGCAGACTAATGGATCTATGCAGGTGGAGGAGGCAGAGGGTGTTGTTCACGTTGTCAGATCCTTCAGATCGGGTTCGTATTTGGAAGGGCGCTCCATGGGGGTATAATCATGCACCGGTGGCGCTGGCTTGCTATGACGGCGTCACACCTATGGAGAAGGTCGTCCCTCTGACCATGGCTTCATATTGGATAACCCTCCAAGATATTCCTCCAGCTTTCAGATCCGAAAGGGTGATGACTATGATTGGTTACACCCTTGGGGATTTTCAGGAGATTGACAAGGTTGGGAAGAAGGCCGGCAAACTCCGCATCCGAGTGGAGATTCCGTTGACCAAGTCGCTGCCCTTCAAGCGGTGGTACTGGGTGGAGGACGAGGTCAAGTTCCTGTGTAAGTTTAAATATGATAAACTGTTTGGTCGTTGCTCCATTTGTGGCTTGGTGACTCACGCTGACCAAGCCGCTGCCCTTCAAGCGGTGGTACTGGGTGGAGGACGAGAGGAAGATGAGGATGGGGCGGAGAGAACTGTTTTAGGGGCACAACAAGCAGCTGCAGTGTCGGTGATAGCTGGCGGGGTACAACTGCAGTCGGGGGCTTCCAGTTCGGGGAGGTCTGTTGCCGGTGTCTCGGGATTGCAAGCTAGGGTTTTGGCTCATAGTCAAACCCTAGTTTTCAAGACTCCTCTTCCAAGTACAATTTTGGAACTAGCTTTGAGTTCCTTCTTGACTAGACCTCAACATCAAGTGGTGATTCGAGAGTTGCAGGAAGCTGCCATGACACCAAAAAGGATTACTGGGAAGCATAGAGGTTGCTCTGATGATGGAGTGACTATATCTCCAAAGAAACTGAAACTTAGTTTGGCCGTGGGCAAGCGCAACCTGAATTTGGAGCCGAAAAGCCTTGGCTTGATTGGTTCCTATGCCGAAGGAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

312

Amino Acids

34.32

Weight (kDa)

9.63

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 113 - 154 9.5e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 18
Acc36I ACCTGC 1 cut(s) 18
AciI CCGC 6 cut(s) 313, 358, 465, 479, 521, 576
AclWI GGATC 3 cut(s) 27, 59, 222
AcoI YGGCCR 1 cut(s) 864
AcsI RAATTY 1 cut(s) 886
AcuI CTGAAG 1 cut(s) 54
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 4 cut(s) 365, 486, 582, 697
AfiI CCNNNNNNNGG 5 cut(s) 357, 478, 609, 889, 933
AgeI ACCGGT 1 cut(s) 121
AgsI TTSAA 3 cut(s) 355, 476, 679
AjuI GAANNNNNNNTTGG 2 cut(s) 845, 877
AluBI AGCT 6 cut(s) 221, 554, 572, 644, 713, 773
AluI AGCT 6 cut(s) 221, 554, 572, 644, 713, 773
Alw26I GTCTC 1 cut(s) 634
AlwI GGATC 3 cut(s) 27, 59, 222
Ama87I CYCGRG 1 cut(s) 631
AoxI GGCC 2 cut(s) 300, 864
ApeKI GCWGC 5 cut(s) 346, 467, 551, 554, 773
ApoI RAATTY 1 cut(s) 886
AsiGI ACCGGT 1 cut(s) 121
AspLEI GCGC 3 cut(s) 98, 130, 879
AsuHPI GGTGA 4 cut(s) 250, 457, 577, 763
AvaI CYCGRG 1 cut(s) 631
BaeGI GKGCMC 1 cut(s) 544
BbvI GCAGC 5 cut(s) 333, 454, 541, 563, 760
BccI CCATC 2 cut(s) 509, 818
BceAI ACGGC 2 cut(s) 160, 851
BcoDI GTCTC 1 cut(s) 634
BfaI CTAG 4 cut(s) 645, 671, 710, 732
BfmI CTRYAG 2 cut(s) 555, 587
BfoI RGCGCY 2 cut(s) 99, 131
BfuAI ACCTGC 1 cut(s) 18
BisI GCNGC 6 cut(s) 347, 465, 468, 552, 555, 774
BlsI GCNGC 6 cut(s) 348, 466, 469, 553, 556, 775
BmeT110I CYCGRG 1 cut(s) 631
BmiI GGNNCC 2 cut(s) 894, 919
BmrI ACTGGG 3 cut(s) 376, 497, 807
BmsI GCATC 1 cut(s) 324
BmuI ACTGGG 3 cut(s) 376, 497, 807
BpmI CTGGAG 1 cut(s) 201
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 5 cut(s) 101, 180, 262, 867, 904
BsaWI WCCGGW 1 cut(s) 121
BsaXI ACNNNNNCTCC 4 cut(s) 317, 347, 823, 853
Bsc4I CCNNNNNNNGG 5 cut(s) 357, 478, 609, 889, 933
Bse118I RCCGGY 3 cut(s) 121, 302, 623
Bse1I ACTGG 4 cut(s) 371, 492, 603, 802
BseDI CCNNGG 5 cut(s) 101, 180, 262, 867, 904
BseGI GGATG 2 cut(s) 315, 520
BseLI CCNNNNNNNGG 5 cut(s) 357, 478, 609, 889, 933
BseNI ACTGG 4 cut(s) 371, 492, 603, 802
BseRI GAGGAG 2 cut(s) 49, 675
BseSI GKGCMC 1 cut(s) 544
BseXI GCAGC 5 cut(s) 333, 454, 541, 563, 760
BshFI GGCC 2 cut(s) 302, 866
BshTI ACCGGT 1 cut(s) 121
BsiHKCI CYCGRG 1 cut(s) 631
BsiSI CCGG 3 cut(s) 122, 303, 624
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 5 cut(s) 357, 478, 609, 889, 933
BsmAI GTCTC 1 cut(s) 634
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 2 cut(s) 302, 866
BsoBI CYCGRG 1 cut(s) 631
Bsp1286I GDGCHC 1 cut(s) 544
Bsp143I GATC 4 cut(s) 19, 64, 73, 227
Bsp19I CCATGG 2 cut(s) 101, 180
BspACI CCGC 6 cut(s) 313, 358, 465, 479, 521, 576
BspANI GGCC 2 cut(s) 302, 866
BspLI GGNNCC 2 cut(s) 894, 919
BspMAI CTGCAG 2 cut(s) 559, 591
BspMI ACCTGC 1 cut(s) 18
BspPI GGATC 3 cut(s) 27, 59, 222
BsrFI RCCGGY 3 cut(s) 121, 302, 623
BsrI ACTGG 4 cut(s) 371, 492, 603, 802
BssAI RCCGGY 3 cut(s) 121, 302, 623
BssECI CCNNGG 5 cut(s) 101, 180, 262, 867, 904
BssMI GATC 4 cut(s) 19, 64, 73, 227
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 4 cut(s) 101, 180, 262, 904
Bst4CI ACNGT 2 cut(s) 417, 532
Bst6I CTCTTC 1 cut(s) 693
BstACI GRCGYC 1 cut(s) 147
BstC8I GCNNGC 6 cut(s) 132, 136, 304, 574, 642, 875
BstDEI CTNAG 1 cut(s) 857
BstDSI CCRYGG 3 cut(s) 101, 180, 867
BstF5I GGATG 2 cut(s) 315, 520
BstH2I RGCGCY 2 cut(s) 99, 131
BstHHI GCGC 3 cut(s) 98, 130, 879
BstKTI GATC 4 cut(s) 22, 67, 76, 230
BstMAI GTCTC 1 cut(s) 634
BstMBI GATC 4 cut(s) 19, 64, 73, 227
BstMWI GCNNNNNNNGC 7 cut(s) 125, 144, 312, 355, 461, 476, 548
BstSFI CTRYAG 2 cut(s) 555, 587
BstSLI GKGCMC 1 cut(s) 544
BstV1I GCAGC 5 cut(s) 333, 454, 541, 563, 760
BstX2I RGATCY 3 cut(s) 19, 64, 227
BstYI RGATCY 3 cut(s) 19, 64, 227
BsuRI GGCC 2 cut(s) 302, 866
BtgI CCRYGG 3 cut(s) 101, 180, 867
BtsCI GGATG 2 cut(s) 315, 520
BtsI GCAGTG 1 cut(s) 564
BtsIMutI CAGTG 1 cut(s) 564
BveI ACCTGC 1 cut(s) 18
Cac8I GCNNGC 6 cut(s) 132, 136, 304, 574, 642, 875
CfoI GCGC 3 cut(s) 98, 130, 879
Cfr10I RCCGGY 3 cut(s) 121, 302, 623
CseI GACGC 1 cut(s) 136
Csp6I GTAC 4 cut(s) 364, 485, 581, 696
CspAI ACCGGT 1 cut(s) 121
CviAII CATG 5 cut(s) 5, 102, 116, 181, 778
CviQI GTAC 4 cut(s) 364, 485, 581, 696
DdeI CTNAG 1 cut(s) 857
DpnI GATC 4 cut(s) 21, 66, 75, 229
DpnII GATC 4 cut(s) 19, 64, 73, 227
DraI TTTAAA 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 864
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
EciI GGCGGA 1 cut(s) 536
Eco130I CCWWGG 4 cut(s) 101, 180, 262, 904
Eco57I CTGAAG 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 631
EcoT14I CCWWGG 4 cut(s) 101, 180, 262, 904
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 4 cut(s) 101, 180, 262, 904
FaeI CATG 5 cut(s) 8, 105, 119, 184, 781
FaqI GGGAC 1 cut(s) 155
FatI CATG 5 cut(s) 4, 101, 115, 180, 777
FauI CCCGC 1 cut(s) 569
Fnu4HI GCNGC 6 cut(s) 347, 465, 468, 552, 555, 774
FokI GGATG 2 cut(s) 302, 527
Fsp4HI GCNGC 6 cut(s) 347, 465, 468, 552, 555, 774
FspBI CTAG 4 cut(s) 645, 671, 710, 732
GlaI GCGC 3 cut(s) 97, 129, 878
GluI GCNGC 6 cut(s) 347, 465, 468, 552, 555, 774
GsuI CTGGAG 1 cut(s) 201
HaeII RGCGCY 2 cut(s) 99, 131
HaeIII GGCC 2 cut(s) 302, 866
HapII CCGG 3 cut(s) 122, 303, 624
HgaI GACGC 1 cut(s) 136
HhaI GCGC 3 cut(s) 98, 130, 879
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 5 cut(s) 8, 105, 119, 184, 781
Hin6I GCGC 3 cut(s) 96, 128, 877
HinP1I GCGC 3 cut(s) 96, 128, 877
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HinfI GANTC 4 cut(s) 328, 448, 682, 754
HpaII CCGG 3 cut(s) 122, 303, 624
HphI GGTGA 4 cut(s) 250, 457, 577, 763
Hpy166II GTNNAC 2 cut(s) 54, 336
Hpy188I TCNGA 7 cut(s) 64, 73, 177, 227, 232, 320, 820
Hpy188III TCNNGA 5 cut(s) 275, 633, 679, 727, 758
Hpy8I GTNNAC 2 cut(s) 54, 336
HpyAV CCTTC 9 cut(s) 78, 85, 157, 292, 361, 482, 733, 923, 927
HpyCH4III ACNGT 2 cut(s) 417, 532
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 7 cut(s) 4, 27, 119, 557, 589, 640, 766
HpyF10VI GCNNNNNNNGC 7 cut(s) 125, 144, 312, 355, 461, 476, 548
HpyF3I CTNAG 1 cut(s) 857
HpySE526I ACGT 1 cut(s) 56
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 5 cut(s) 8, 105, 119, 184, 781
HspAI GCGC 3 cut(s) 96, 128, 877
KroI GCCGGC 1 cut(s) 302
KroNI GCCGGC 1 cut(s) 304
Kzo9I GATC 4 cut(s) 19, 64, 73, 227
LmnI GCTCC 3 cut(s) 103, 434, 892
Lsp1109I GCAGC 5 cut(s) 333, 454, 541, 563, 760
LweI GCATC 1 cut(s) 324
MaeI CTAG 4 cut(s) 645, 671, 710, 732
MaeII ACGT 1 cut(s) 56
MaeIII GTNAC 4 cut(s) 148, 254, 445, 829
MalI GATC 4 cut(s) 21, 66, 75, 229
MboI GATC 4 cut(s) 19, 64, 73, 227
MboII GAAGA 3 cut(s) 307, 518, 680
MflI RGATCY 3 cut(s) 19, 64, 227
MhlI GDGCHC 1 cut(s) 544
MluCI AATT 2 cut(s) 699, 886
MlyI GAGTC 2 cut(s) 442, 676
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 302
MseI TTAA 1 cut(s) 402
MslI CAYNNNNRTG 2 cut(s) 155, 320
MspA1I CMGCKG 2 cut(s) 467, 554
MspI CCGG 3 cut(s) 122, 303, 624
MwoI GCNNNNNNNGC 7 cut(s) 125, 144, 312, 355, 461, 476, 548
NaeI GCCGGC 1 cut(s) 304
NcoI CCATGG 2 cut(s) 101, 180
NdeII GATC 4 cut(s) 19, 64, 73, 227
NgoMIV GCCGGC 1 cut(s) 302
NlaIII CATG 5 cut(s) 8, 105, 119, 184, 781
NlaIV GGNNCC 2 cut(s) 894, 919
NmuCI GTSAC 3 cut(s) 148, 445, 829
NsiI ATGCAT 1 cut(s) 6
PaqCI CACCTGC 1 cut(s) 18
PdiI GCCGGC 1 cut(s) 304
PfeI GAWTC 2 cut(s) 328, 754
PflFI GACNNNGTC 3 cut(s) 146, 340, 380
PinAI ACCGGT 1 cut(s) 121
PkrI GCNGC 6 cut(s) 348, 466, 469, 553, 556, 775
PleI GAGTC 2 cut(s) 442, 676
PpsI GAGTC 2 cut(s) 442, 676
PspN4I GGNNCC 2 cut(s) 894, 919
PstI CTGCAG 2 cut(s) 559, 591
PsuI RGATCY 3 cut(s) 19, 64, 227
PsyI GACNNNGTC 3 cut(s) 146, 340, 380
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 4 cut(s) 365, 486, 582, 697
RsaNI GTAC 4 cut(s) 364, 485, 581, 696
RseI CAYNNNNRTG 2 cut(s) 155, 320
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 6 cut(s) 347, 465, 468, 552, 555, 774
Sau3AI GATC 4 cut(s) 19, 64, 73, 227
SchI GAGTC 2 cut(s) 442, 676
SduI GDGCHC 1 cut(s) 544
SfaNI GCATC 1 cut(s) 324
SfcI CTRYAG 2 cut(s) 555, 587
SgrAI CRCCGGYG 1 cut(s) 121
SmiMI CAYNNNNRTG 2 cut(s) 155, 320
Sse9I AATT 2 cut(s) 699, 886
SsiI CCGC 6 cut(s) 313, 358, 465, 479, 521, 576
SspMI CTAG 4 cut(s) 645, 671, 710, 732
StyI CCWWGG 4 cut(s) 101, 180, 262, 904
TaaI ACNGT 2 cut(s) 417, 532
TaiI ACGT 1 cut(s) 59
TaqI TCGA 1 cut(s) 757
TasI AATT 2 cut(s) 699, 886
TatI WGTACW 1 cut(s) 695
TauI GCSGC 1 cut(s) 467
TfiI GAWTC 2 cut(s) 328, 754
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TscAI CASTG 1 cut(s) 564
TseFI GTSAC 3 cut(s) 148, 445, 829
TseI GCWGC 5 cut(s) 346, 467, 551, 554, 773
Tsp45I GTSAC 3 cut(s) 148, 445, 829
TspDTI ATGAA 1 cut(s) 177
TspGWI ACGGA 1 cut(s) 321
TspRI CASTG 1 cut(s) 564
Tth111I GACNNNGTC 3 cut(s) 146, 340, 380
XapI RAATTY 1 cut(s) 886
XcmI CCANNNNNNNNNTGG 1 cut(s) 439
XspI CTAG 4 cut(s) 645, 671, 710, 732
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.