Rroxscaffold_2G00106510

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001316
Physical Location & Seq
Reverse (-)
30107012 .. 30110319
3308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00106510.1

Sequence Viewer

Length: 1878 bp
ATGTTTCAGAATGAGCAACGTATGAAATCAATGGCTATCATAGAAAGGATCATACTTGTTTGTATTTTGTGCTGCATACTCTTAATGGAGGCCTCTGACTCTCAATCAGATGCCTTAGCACAAGGCCAAGAGCTCAAGGATGGGATGCAGCTTTTTTCAGCATCTGGGATTTTCAGGTTAGGATTCTTTAAGCCTGGGGATGCAAGTAACTCTTACTTAGGTATAAGGTACAACAGAAATAATGAAAAAGCAGTATGGGTTGCCAACCGAAACAATTCGATTCTTGATAATTCTGGAGTTCTTAGAATTGATCAGTATGGTAACTTGAAGATTACACAGAAGATGATAGATGATATTGTACTATATTCAGTTAAAGAAGCAATCAATGCCAGTGCTATTCTTCTTGACAGTGGAAATTTTGTGCTTAGTGAGTTGAACGCTGATGGGTCTATAAAGCAGGAGTTGTGGCAAAGCTTTGATTATCCAACTGACACACTTTTGCCAAAAATGAAGTTGGGATTTAATAGAAAAACTGGCCTCAATTGGACACTCAAATCATGGAGAACTGGAAATTTGCCTAGCATAGGGTCCTTTACACTTGGTTTGGACCCTAGTGGTGTTAAACAAATGGTCATTTCTTGGAGAGGAAATCTGTACTGGACTAGTGGATCCTGGCACACATGGTGCTTCAACCATACAGATGAGTTTTGCAGTAACTATAAGTACAACTTTAGCTACATATCAAATGAGAATGAAACATACTTGAGTTATTCAGTAGATAAAGGTACCACTATTTTCCCAAGACTCCTGTTAAATGAAGAAGGTGAACTCAGAGGTTTCGGAATGGATGCCATGTTTACTGGAGTTTCATGCACTTCTTCCACCAACTCTACCTTGAAAAATGGCTGTGTTAAGCAAAGGCAACCAGACTGCAGGAGCTCTCATGACAAATTCGTGCAAAAACTTGGTGTCATGTCTCGCAGTGGAATCAAGATCCTTGAGAATGAAAACATGACTCTGATTGATTGTTGGGATGTTTGCTTCAAAATGTGTTCTTGTCTTGCTTATGCTTCTGCAAATGATGATGGCACCGGCTGCAAGATTTGGAAGGAAGGAGCAAGTTTCACACAATACAAATTGGGTAGTCTGAGAGAGATACACATCCTGGAATCCAAAGATGCTCAAAGAAAGAATGTCTTAGACTGGAAGAAACGCTTCAGCATCATAGAGGGGATTGCTCAAGGACTAATCTATCTTCATAAATACTCGAGACTAAAAGTGATTCACAGGGATTTAAAAGCCAGCAATATTTTACTCGACAAGAATCTAAATCCTAAAGTATCAGATTTTGGCATGGCTAGGATATGTGGGTTGAATGAGTCAGAAGAAAACACAAATAGAGTTGTTGGAACATATGGTTATATGTCTCCGGAGTATGCCATGAAAGGCATTGTGTCCATTAAAACTGATATTTTCAGCTATGGAGTCCTACTGCTGGAAATTGTAAGTGGGAAGAAGAATAACAGTAACTACCATTCAGAGCACCAACTCAACCTCATTGGATATGCATGGCAGCTTTGGAATGAAGATAGAGGATCTGAGCTAGCAGACCCAGGATTAGGTGAATCATGCCCCATTACTGAAGTTCTAAGATGCATTCTTGTGGGTCTTTTGTGTGTACAAGACCATGCAGCAGACAGGCCAACAATGCCGGATGTTGTTTCCATGCTTTTAAATCAAAGCATTCTATTACCTCCTCCAAAACAACCAGCATTTTTTATAAATACAGTTGAGAAAGAGTTTATAAAGGCATCAGGAAAGGAGTCCGAAATTTGTTCCGCAAATGATGTAACTATATCAGTAATGGAAGCAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr

Protein Analysis

625

Amino Acids

70.28

Weight (kDa)

6.54

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 85 - 188 7.2e-29 D-mannose binding lectin
PAN_2 PF08276 311 - 371 4.3e-08 PAN-like domain
PK_Tyr_Ser-Thr PF07714 392 - 519 1.8e-24 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 396 - 516 1.4e-25 Protein kinase domain
DUF3403 PF11883 584 - 625 5.5e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1781, 1805
Acc65I GGTACC 1 cut(s) 785
AccB1I GGYRCC 2 cut(s) 785, 1088
AccIII TCCGGA 1 cut(s) 1429
AciI CCGC 1 cut(s) 1839
AclWI GGATC 5 cut(s) 56, 663, 676, 988, 1603
AcsI RAATTY 4 cut(s) 415, 571, 950, 1830
AcuI CTGAAG 2 cut(s) 1201, 1662
AdeI CACNNNGTG 1 cut(s) 684
AfaI GTAC 6 cut(s) 230, 360, 656, 725, 787, 1680
AfiI CCNNNNNNNGG 3 cut(s) 584, 1495, 1619
AgsI TTSAA 6 cut(s) 328, 436, 691, 898, 1045, 1375
AhlI ACTAGT 1 cut(s) 662
AjnI CCWGG 4 cut(s) 193, 671, 1164, 1612
AluBI AGCT 8 cut(s) 133, 151, 474, 735, 939, 1479, 1576, 1603
AluI AGCT 8 cut(s) 133, 151, 474, 735, 939, 1479, 1576, 1603
Alw21I GWGCWC 3 cut(s) 135, 941, 1545
Alw26I GTCTC 3 cut(s) 981, 1264, 1431
AlwI GGATC 5 cut(s) 56, 663, 676, 988, 1603
AlwNI CAGNNNCTG 1 cut(s) 164
Ama87I CYCGRG 1 cut(s) 1267
Aor13HI TCCGGA 1 cut(s) 1429
AoxI GGCC 4 cut(s) 90, 124, 535, 1700
ApeKI GCWGC 5 cut(s) 72, 148, 1095, 1573, 1691
ApoI RAATTY 4 cut(s) 415, 571, 950, 1830
Asp700I GAANNNNTTC 2 cut(s) 274, 1214
Asp718I GGTACC 1 cut(s) 785
AspS9I GGNCC 2 cut(s) 588, 607
AsuHPI GGTGA 2 cut(s) 836, 1634
AsuNHI GCTAGC 1 cut(s) 1603
AvaI CYCGRG 1 cut(s) 1267
AvaII GGWCC 2 cut(s) 588, 607
BamHI GGATCC 1 cut(s) 668
BanI GGYRCC 2 cut(s) 785, 1088
BanII GRGCYC 2 cut(s) 135, 941
Bbv12I GWGCWC 3 cut(s) 135, 941, 1545
BbvI GCAGC 5 cut(s) 59, 160, 1082, 1585, 1703
BccI CCATC 3 cut(s) 134, 437, 1079
BciT130I CCWGG 4 cut(s) 195, 673, 1166, 1614
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 3 cut(s) 981, 1264, 1431
BcuI ACTAGT 1 cut(s) 662
BfaI CTAG 5 cut(s) 579, 612, 663, 1359, 1604
BfmI CTRYAG 1 cut(s) 931
BisI GCNGC 5 cut(s) 73, 149, 1096, 1574, 1692
BlsI GCNGC 5 cut(s) 74, 150, 1097, 1575, 1693
Bme1390I CCNGG 4 cut(s) 195, 673, 1166, 1614
Bme18I GGWCC 2 cut(s) 588, 607
BmeT110I CYCGRG 1 cut(s) 1267
BmgT120I GGNCC 2 cut(s) 588, 607
BmiI GGNNCC 5 cut(s) 589, 609, 670, 787, 1090
BmrFI CCNGG 4 cut(s) 195, 673, 1166, 1614
BmsI GCATC 9 cut(s) 100, 135, 170, 190, 838, 1168, 1230, 1643, 1820
BmtI GCTAGC 1 cut(s) 1607
BpmI CTGGAG 2 cut(s) 315, 882
Bpu10I CCTNAGC 1 cut(s) 115
BpuEI CTTGAG 4 cut(s) 119, 784, 1019, 1224
BsaBI GATNNNNATC 1 cut(s) 1160
BsaJI CCNNGG 2 cut(s) 194, 1612
BsaWI WCCGGW 1 cut(s) 1429
Bsc4I CCNNNNNNNGG 3 cut(s) 584, 1495, 1619
Bse118I RCCGGY 1 cut(s) 1091
Bse1I ACTGG 6 cut(s) 390, 538, 571, 662, 865, 1208
Bse8I GATNNNNATC 1 cut(s) 1160
BseAI TCCGGA 1 cut(s) 1429
BseBI CCWGG 4 cut(s) 195, 673, 1166, 1614
BseDI CCNNGG 2 cut(s) 194, 1612
BseGI GGATG 7 cut(s) 145, 150, 205, 853, 1039, 1161, 1720
BseJI GATNNNNATC 1 cut(s) 1160
BseLI CCNNNNNNNGG 3 cut(s) 584, 1495, 1619
BseMII CTCAG 3 cut(s) 844, 1139, 1590
BseNI ACTGG 6 cut(s) 390, 538, 571, 662, 865, 1208
BseRI GAGGAG 1 cut(s) 1746
BseXI GCAGC 5 cut(s) 59, 160, 1082, 1585, 1703
BshFI GGCC 4 cut(s) 92, 126, 537, 1702
BshNI GGYRCC 2 cut(s) 785, 1088
BsiHKAI GWGCWC 3 cut(s) 135, 941, 1545
BsiHKCI CYCGRG 1 cut(s) 1267
BsiSI CCGG 3 cut(s) 1092, 1430, 1712
BslI CCNNNNNNNGG 3 cut(s) 584, 1495, 1619
BsmAI GTCTC 3 cut(s) 981, 1264, 1431
BsmI GAATGC 2 cut(s) 1656, 1743
BsnI GGCC 4 cut(s) 92, 126, 537, 1702
BsoBI CYCGRG 1 cut(s) 1267
Bsp1286I GDGCHC 3 cut(s) 135, 941, 1545
Bsp13I TCCGGA 1 cut(s) 1429
Bsp1407I TGTACA 1 cut(s) 1678
Bsp143I GATC 5 cut(s) 48, 310, 668, 993, 1595
BspACI CCGC 1 cut(s) 1839
BspANI GGCC 4 cut(s) 92, 126, 537, 1702
BspCNI CTCAG 3 cut(s) 843, 1140, 1591
BspEI TCCGGA 1 cut(s) 1429
BspHI TCATGA 1 cut(s) 943
BspLI GGNNCC 5 cut(s) 589, 609, 670, 787, 1090
BspMAI CTGCAG 1 cut(s) 935
BspOI GCTAGC 1 cut(s) 1607
BspPI GGATC 5 cut(s) 56, 663, 676, 988, 1603
BspT107I GGYRCC 2 cut(s) 785, 1088
BsrFI RCCGGY 1 cut(s) 1091
BsrGI TGTACA 1 cut(s) 1678
BsrI ACTGG 6 cut(s) 390, 538, 571, 662, 865, 1208
BssAI RCCGGY 1 cut(s) 1091
BssECI CCNNGG 2 cut(s) 194, 1612
BssMI GATC 5 cut(s) 48, 310, 668, 993, 1595
Bst2UI CCWGG 4 cut(s) 195, 673, 1166, 1614
Bst4CI ACNGT 3 cut(s) 410, 1526, 1789
BstAPI GCANNNNNTGC 2 cut(s) 386, 1095
BstAUI TGTACA 1 cut(s) 1678
BstC8I GCNNGC 2 cut(s) 1303, 1605
BstDEI CTNAG 9 cut(s) 115, 217, 302, 425, 830, 1148, 1198, 1599, 1649
BstENI CCTNNNNNAGG 1 cut(s) 582
BstF5I GGATG 7 cut(s) 145, 150, 205, 853, 1039, 1161, 1720
BstKTI GATC 5 cut(s) 51, 313, 671, 996, 1598
BstMAI GTCTC 3 cut(s) 981, 1264, 1431
BstMBI GATC 5 cut(s) 48, 310, 668, 993, 1595
BstMWI GCNNNNNNNGC 3 cut(s) 386, 1095, 1708
BstNI CCWGG 4 cut(s) 195, 673, 1166, 1614
BstSCI CCNGG 4 cut(s) 193, 671, 1164, 1612
BstSFI CTRYAG 1 cut(s) 931
BstV1I GCAGC 5 cut(s) 59, 160, 1082, 1585, 1703
BstX2I RGATCY 3 cut(s) 668, 993, 1595
BstYI RGATCY 3 cut(s) 668, 993, 1595
BsuRI GGCC 4 cut(s) 92, 126, 537, 1702
BtsCI GGATG 7 cut(s) 145, 150, 205, 853, 1039, 1161, 1720
BtsI GCAGTG 1 cut(s) 988
BtsIMutI CAGTG 3 cut(s) 397, 415, 988
Cac8I GCNNGC 2 cut(s) 1303, 1605
CaiI CAGNNNCTG 1 cut(s) 164
CciI TCATGA 1 cut(s) 943
Cfr10I RCCGGY 1 cut(s) 1091
Cfr13I GGNCC 2 cut(s) 588, 607
Csp6I GTAC 6 cut(s) 229, 359, 655, 724, 786, 1679
CviQI GTAC 6 cut(s) 229, 359, 655, 724, 786, 1679
DdeI CTNAG 9 cut(s) 115, 217, 302, 425, 830, 1148, 1198, 1599, 1649
DpnI GATC 5 cut(s) 50, 312, 670, 995, 1597
DpnII GATC 5 cut(s) 48, 310, 668, 993, 1595
DraI TTTAAA 2 cut(s) 1296, 1734
DraIII CACNNNGTG 1 cut(s) 684
Ecl136II GAGCTC 2 cut(s) 133, 939
Eco147I AGGCCT 1 cut(s) 92
Eco24I GRGCYC 2 cut(s) 135, 941
Eco47I GGWCC 2 cut(s) 588, 607
Eco53kI GAGCTC 2 cut(s) 133, 939
Eco57I CTGAAG 2 cut(s) 1201, 1662
Eco88I CYCGRG 1 cut(s) 1267
EcoICRI GAGCTC 2 cut(s) 133, 939
EcoNI CCTNNNNNAGG 1 cut(s) 582
EcoO109I RGGNCCY 1 cut(s) 588
EcoRII CCWGG 4 cut(s) 193, 671, 1164, 1612
EcoT22I ATGCAT 2 cut(s) 1570, 1658
EcoT38I GRGCYC 2 cut(s) 135, 941
FalI AAGNNNNNCTT 8 cut(s) 196, 228, 713, 745, 1181, 1213, 1199, 1231
FauNDI CATATG 1 cut(s) 1414
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 5 cut(s) 73, 149, 1096, 1574, 1692
FokI GGATG 7 cut(s) 152, 157, 212, 860, 1046, 1148, 1727
FriOI GRGCYC 2 cut(s) 135, 941
Fsp4HI GCNGC 5 cut(s) 73, 149, 1096, 1574, 1692
FspBI CTAG 5 cut(s) 579, 612, 663, 1359, 1604
GluI GCNGC 5 cut(s) 73, 149, 1096, 1574, 1692
GsuI CTGGAG 2 cut(s) 315, 882
HaeIII GGCC 4 cut(s) 92, 126, 537, 1702
HapII CCGG 3 cut(s) 1092, 1430, 1712
HindIII AAGCTT 1 cut(s) 472
HpaII CCGG 3 cut(s) 1092, 1430, 1712
HphI GGTGA 2 cut(s) 836, 1634
Hpy166II GTNNAC 3 cut(s) 827, 858, 1679
Hpy188III TCNNGA 8 cut(s) 284, 294, 404, 944, 991, 1269, 1430, 1815
Hpy8I GTNNAC 3 cut(s) 827, 858, 1679
HpyAV CCTTC 3 cut(s) 815, 1102, 1106
HpyCH4III ACNGT 3 cut(s) 410, 1526, 1789
HpyCH4IV ACGT 1 cut(s) 19
HpyF10VI GCNNNNNNNGC 3 cut(s) 386, 1095, 1708
HpyF3I CTNAG 9 cut(s) 115, 217, 302, 425, 830, 1148, 1198, 1599, 1649
HpySE526I ACGT 1 cut(s) 19
Kpn2I TCCGGA 1 cut(s) 1429
KpnI GGTACC 1 cut(s) 789
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 5 cut(s) 48, 310, 668, 993, 1595
LmnI GCTCC 2 cut(s) 936, 1115
Lsp1109I GCAGC 5 cut(s) 59, 160, 1082, 1585, 1703
LweI GCATC 9 cut(s) 100, 135, 170, 190, 838, 1168, 1230, 1643, 1820
MaeI CTAG 5 cut(s) 579, 612, 663, 1359, 1604
MaeII ACGT 1 cut(s) 19
MaeIII GTNAC 5 cut(s) 206, 320, 713, 1526, 1849
MalI GATC 5 cut(s) 50, 312, 670, 995, 1597
MboI GATC 5 cut(s) 48, 310, 668, 993, 1595
MfeI CAATTG 1 cut(s) 541
MflI RGATCY 3 cut(s) 668, 993, 1595
MhlI GDGCHC 3 cut(s) 135, 941, 1545
MlyI GAGTC 6 cut(s) 92, 798, 1009, 1388, 1494, 1832
MmeI TCCRAC 2 cut(s) 509, 1387
Mph1103I ATGCAT 2 cut(s) 1570, 1658
MroI TCCGGA 1 cut(s) 1429
MroXI GAANNNNTTC 2 cut(s) 274, 1214
MslI CAYNNNNRTG 2 cut(s) 699, 1661
MspI CCGG 3 cut(s) 1092, 1430, 1712
MspR9I CCNGG 4 cut(s) 195, 673, 1166, 1614
MunI CAATTG 1 cut(s) 541
Mva1269I GAATGC 2 cut(s) 1656, 1743
MvaI CCWGG 4 cut(s) 195, 673, 1166, 1614
MwoI GCNNNNNNNGC 3 cut(s) 386, 1095, 1708
NdeI CATATG 1 cut(s) 1414
NdeII GATC 5 cut(s) 48, 310, 668, 993, 1595
NheI GCTAGC 1 cut(s) 1603
NlaIV GGNNCC 5 cut(s) 589, 609, 670, 787, 1090
NsiI ATGCAT 2 cut(s) 1570, 1658
PaeR7I CTCGAG 1 cut(s) 1267
PagI TCATGA 1 cut(s) 943
PceI AGGCCT 1 cut(s) 92
PctI GAATGC 2 cut(s) 1656, 1743
PdmI GAANNNNTTC 2 cut(s) 274, 1214
PfeI GAWTC 7 cut(s) 183, 280, 987, 1169, 1282, 1324, 1625
PfoI TCCNGGA 1 cut(s) 1164
PkrI GCNGC 5 cut(s) 74, 150, 1097, 1575, 1693
PleI GAGTC 6 cut(s) 92, 798, 1009, 1387, 1493, 1831
PpsI GAGTC 6 cut(s) 92, 798, 1009, 1387, 1493, 1831
PpuMI RGGWCCY 1 cut(s) 588
PsiI TTATAA 2 cut(s) 1781, 1805
Psp124BI GAGCTC 2 cut(s) 135, 941
Psp5II RGGWCCY 1 cut(s) 588
Psp6I CCWGG 4 cut(s) 193, 671, 1164, 1612
PspGI CCWGG 4 cut(s) 193, 671, 1164, 1612
PspN4I GGNNCC 5 cut(s) 589, 609, 670, 787, 1090
PspPI GGNCC 2 cut(s) 588, 607
PspPPI RGGWCCY 1 cut(s) 588
PstI CTGCAG 1 cut(s) 935
PstNI CAGNNNCTG 1 cut(s) 164
PsuI RGATCY 3 cut(s) 668, 993, 1595
RsaI GTAC 6 cut(s) 230, 360, 656, 725, 787, 1680
RsaNI GTAC 6 cut(s) 229, 359, 655, 724, 786, 1679
RseI CAYNNNNRTG 2 cut(s) 699, 1661
SacI GAGCTC 2 cut(s) 135, 941
SatI GCNGC 5 cut(s) 73, 149, 1096, 1574, 1692
Sau3AI GATC 5 cut(s) 48, 310, 668, 993, 1595
Sau96I GGNCC 2 cut(s) 588, 607
SchI GAGTC 6 cut(s) 92, 798, 1009, 1388, 1494, 1832
ScrFI CCNGG 4 cut(s) 195, 673, 1166, 1614
SduI GDGCHC 3 cut(s) 135, 941, 1545
SfaNI GCATC 9 cut(s) 100, 135, 170, 190, 838, 1168, 1230, 1643, 1820
SfcI CTRYAG 1 cut(s) 931
Sfr274I CTCGAG 1 cut(s) 1267
SinI GGWCC 2 cut(s) 588, 607
SlaI CTCGAG 1 cut(s) 1267
SmiMI CAYNNNNRTG 2 cut(s) 699, 1661
SmlI CTYRAG 5 cut(s) 134, 763, 998, 1239, 1267
SmoI CTYRAG 5 cut(s) 134, 763, 998, 1239, 1267
SpeI ACTAGT 1 cut(s) 662
SseBI AGGCCT 1 cut(s) 92
SsiI CCGC 1 cut(s) 1839
SspI AATATT 1 cut(s) 1309
SspMI CTAG 5 cut(s) 579, 612, 663, 1359, 1604
SstI GAGCTC 2 cut(s) 135, 941
StuI AGGCCT 1 cut(s) 92
StyD4I CCNGG 4 cut(s) 193, 671, 1164, 1612
TaaI ACNGT 3 cut(s) 410, 1526, 1789
TaiI ACGT 1 cut(s) 22
TaqI TCGA 3 cut(s) 278, 1268, 1317
TatI WGTACW 4 cut(s) 358, 654, 723, 1678
TfiI GAWTC 7 cut(s) 183, 280, 987, 1169, 1282, 1324, 1625
TscAI CASTG 3 cut(s) 397, 415, 988
TseI GCWGC 5 cut(s) 72, 148, 1095, 1573, 1691
TspRI CASTG 3 cut(s) 397, 415, 988
VpaK11BI GGWCC 2 cut(s) 588, 607
XagI CCTNNNNNAGG 1 cut(s) 582
XapI RAATTY 4 cut(s) 415, 571, 950, 1830
XhoI CTCGAG 1 cut(s) 1267
XmnI GAANNNNTTC 2 cut(s) 274, 1214
XspI CTAG 5 cut(s) 579, 612, 663, 1359, 1604
Zsp2I ATGCAT 2 cut(s) 1570, 1658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.