Rroxscaffold_2G00106540

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001316
Physical Location & Seq
Reverse (-)
30124497 .. 30127815
3319 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00106540.1

Sequence Viewer

Length: 1848 bp
ATGGCCATCATAGAAAGGAGCATACTTGTTTGTATTTTGTGCTGCACATTCTTGATGGAGGCCTCTGACTCTCAATCAGATACCTTAGCACAAGGCCAAGAGCTCAAGGATGGGATGCAGCTTTTTTCAGCATCTGGGATTTTCAGGTTAGGATTCTTTAAGCCTGGCAATGCAAGTAACACTTACTTAGGAATATGGTACAACAGAAATAATGAAAAAGCAGTATGGGTTGCCAACCGAAACAATCCGATTCTTGACAATTCTGGAGTTCTTAGAATTGATCAGTATGGTAACTTGAAGATTACACAGAAGATGGTAGATGATATTGTACTATATTCTGTTAAAGAAGCAATCAATACTAGTGCTATTCTTCTTGACAGTGGAAATTTTGTGCTTAGTGAGTTGAACGCTGATGGGTCTATAAAGCAGGAGTTGTGGCAAAGCTTTGATTATCCAACTGACACACTTTTGCCAAAAATGAAGTTGGGATTTAATAGAAAAACTGGCCTCAATTGGACTCTCAAATCATGGAGAACTGGAAATTTGCCTAGCATAGGGTCCTTTACACTTGGTTTGGACCCTAGTGGTGTTAAACAAATGGTCATTTCTTGGAGAGGAAATCTGTACTGGACTAGTGGATCCTGGCACACTTGGTGCTTCAGCCTTACAGATGAGTTTTGCAGTAACTATAAGTACAACTTTAGCTACATATCAAATGAGAATGAAACATACTTGAGTTATTCAGTAGATAAAGGTACCACTATTTTCCCAAGACTCCTGTTAAATGAAGAAGGTGAACTCAGAGGTTTCAGGATGGATGCCATGTTTACAGGAGTTTCATGCACTTCTTCCACCAACTCTACCTTGAAAAATGGGTGTGTTGAGCAAAGGCAACCAGACTGCAGGAGCTCTCATGACAAATTCGTGCCAAAACTTGGTGTCATGTCTCGCAGTGCAATCAAGATCCTTGAGAATGAAAACATGACTCTGATTGATTGTTGGGATGTTTGCTTCAAAATGTGTTCTTGTCTTGCTTATGCTTCTGCAAATGATGATGGCACCGGCTGCGAGATTTGGAAGAAAGGGGCAAGTTTCACACAAAACAATTTGGGTAGTCTGAGAGAGATACACATCCTGGAATCCAAAGATGCTCAAAGAAAGAATGTCTTAGACTGGAAGAAACGCTTCAGCATCATAGAGGGGATTGCTCAAGGACTAATCTATCTTCATAAATACTCGAGACTAAAAGTGATTCACAGGGATTTAAAAGCCAGCAATATTTTACTCGACAAGAATCTAAATCCCAAAGTATCAGATTTTGGCATGGCTAGGATATGTGGGTTGAATGAGTCAGAAGAAAACACAAATAGAGTTGTTGGAACATATGGTTATATGTCTCCGGAGTATGCCATGAAAGGCATTGTGTCCATTAAAACTGATATTTTCAGCTTTGGAGTCCTACTGCTGGAAATTGTAAGTGGGAAGAAGAATAACAGTAACTACCATTCAGAGGACCAACTCAACCTCATTGGATATGCATGGCAGCTTTGGAATGAAGATAGAGGATCTGAGCTAGCAGACCCAGGATTAGGAGAATCATGCCCCATTGCTGAAGTTCTAAGATGCATTCATGTGGGTCTTTTGTGTGTACAAGACCGTGCAGCAGACAGGCCAACAATGCCGGATGTTGTTTCCATGCTTTTAAATCAAAGCATTCTATTACCTCCTCCAAAACAACCAGCATTTTTTATAAATACAGTTGAGAAAGAGTTTATAAAGGCATCACGAAAGGAGTCTGAAATTTGTTCCACAAATGATGTAACTATATCAGTAATGGAAGCAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr

Protein Analysis

615

Amino Acids

69.17

Weight (kDa)

6.09

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 75 - 178 2.8e-30 D-mannose binding lectin
PAN_2 PF08276 301 - 361 4e-08 PAN-like domain
PK_Tyr_Ser-Thr PF07714 385 - 510 1e-24 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 386 - 505 3.7e-25 Protein kinase domain
DUF3403 PF11883 574 - 615 3.6e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1751, 1775
Acc65I GGTACC 1 cut(s) 755
AccB1I GGYRCC 2 cut(s) 755, 1058
AccB7I CCANNNNNTGG 1 cut(s) 935
AccIII TCCGGA 1 cut(s) 1399
AclWI GGATC 4 cut(s) 633, 646, 958, 1573
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 4 cut(s) 385, 541, 920, 1800
AcuI CTGAAG 3 cut(s) 643, 1171, 1632
AdeI CACNNNGTG 1 cut(s) 654
AfaI GTAC 6 cut(s) 200, 330, 626, 695, 757, 1650
AfiI CCNNNNNNNGG 5 cut(s) 554, 935, 1465, 1510, 1589
AgsI TTSAA 5 cut(s) 298, 406, 868, 1015, 1345
AhlI ACTAGT 2 cut(s) 359, 632
AjnI CCWGG 4 cut(s) 163, 641, 1134, 1582
AluBI AGCT 8 cut(s) 103, 121, 444, 705, 909, 1449, 1546, 1573
AluI AGCT 8 cut(s) 103, 121, 444, 705, 909, 1449, 1546, 1573
Alw21I GWGCWC 2 cut(s) 105, 911
Alw26I GTCTC 3 cut(s) 951, 1234, 1401
AlwI GGATC 4 cut(s) 633, 646, 958, 1573
AlwNI CAGNNNCTG 1 cut(s) 134
Ama87I CYCGRG 1 cut(s) 1237
Aor13HI TCCGGA 1 cut(s) 1399
AoxI GGCC 5 cut(s) 3, 60, 94, 505, 1670
ApeKI GCWGC 5 cut(s) 42, 118, 1065, 1543, 1661
ApoI RAATTY 4 cut(s) 385, 541, 920, 1800
Asp700I GAANNNNTTC 1 cut(s) 1184
Asp718I GGTACC 1 cut(s) 755
AspS9I GGNCC 3 cut(s) 558, 577, 1513
AsuHPI GGTGA 1 cut(s) 806
AsuNHI GCTAGC 1 cut(s) 1573
AvaI CYCGRG 1 cut(s) 1237
AvaII GGWCC 3 cut(s) 558, 577, 1513
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 638
BanI GGYRCC 2 cut(s) 755, 1058
BanII GRGCYC 2 cut(s) 105, 911
Bbv12I GWGCWC 2 cut(s) 105, 911
BbvI GCAGC 5 cut(s) 29, 130, 1052, 1555, 1673
BccI CCATC 7 cut(s) 14, 49, 104, 307, 407, 808, 1049
BciT130I CCWGG 4 cut(s) 165, 643, 1136, 1584
BclI TGATCA 1 cut(s) 280
BcoDI GTCTC 3 cut(s) 951, 1234, 1401
BcuI ACTAGT 2 cut(s) 359, 632
BfaI CTAG 6 cut(s) 360, 549, 582, 633, 1329, 1574
BfmI CTRYAG 1 cut(s) 901
BisI GCNGC 5 cut(s) 43, 119, 1066, 1544, 1662
BlsI GCNGC 5 cut(s) 44, 120, 1067, 1545, 1663
Bme1390I CCNGG 4 cut(s) 165, 643, 1136, 1584
Bme18I GGWCC 3 cut(s) 558, 577, 1513
BmeT110I CYCGRG 1 cut(s) 1237
BmgT120I GGNCC 3 cut(s) 558, 577, 1513
BmiI GGNNCC 5 cut(s) 559, 579, 640, 757, 1060
BmrFI CCNGG 4 cut(s) 165, 643, 1136, 1584
BmsI GCATC 7 cut(s) 105, 140, 808, 1138, 1200, 1613, 1790
BmtI GCTAGC 1 cut(s) 1577
BpmI CTGGAG 1 cut(s) 285
Bpu10I CCTNAGC 1 cut(s) 85
BpuEI CTTGAG 4 cut(s) 89, 754, 989, 1194
BsaBI GATNNNNATC 1 cut(s) 1130
BsaJI CCNNGG 1 cut(s) 1582
BsaWI WCCGGW 1 cut(s) 1399
Bsc4I CCNNNNNNNGG 5 cut(s) 554, 935, 1465, 1510, 1589
Bse118I RCCGGY 1 cut(s) 1061
Bse1I ACTGG 4 cut(s) 508, 541, 632, 1178
Bse3DI GCAATG 2 cut(s) 175, 1605
Bse8I GATNNNNATC 1 cut(s) 1130
BseAI TCCGGA 1 cut(s) 1399
BseBI CCWGG 4 cut(s) 165, 643, 1136, 1584
BseDI CCNNGG 1 cut(s) 1582
BseGI GGATG 7 cut(s) 115, 120, 819, 823, 1009, 1131, 1690
BseJI GATNNNNATC 1 cut(s) 1130
BseLI CCNNNNNNNGG 5 cut(s) 554, 935, 1465, 1510, 1589
BseMI GCAATG 2 cut(s) 175, 1605
BseMII CTCAG 3 cut(s) 814, 1109, 1560
BseNI ACTGG 4 cut(s) 508, 541, 632, 1178
BseRI GAGGAG 1 cut(s) 1716
BseXI GCAGC 5 cut(s) 29, 130, 1052, 1555, 1673
BsgI GTGCAG 2 cut(s) 28, 1680
BshFI GGCC 5 cut(s) 5, 62, 96, 507, 1672
BshNI GGYRCC 2 cut(s) 755, 1058
BsiHKAI GWGCWC 2 cut(s) 105, 911
BsiHKCI CYCGRG 1 cut(s) 1237
BsiSI CCGG 3 cut(s) 1062, 1400, 1682
BslI CCNNNNNNNGG 5 cut(s) 554, 935, 1465, 1510, 1589
BsmAI GTCTC 3 cut(s) 951, 1234, 1401
BsmI GAATGC 2 cut(s) 1626, 1713
BsnI GGCC 5 cut(s) 5, 62, 96, 507, 1672
BsoBI CYCGRG 1 cut(s) 1237
Bsp1286I GDGCHC 2 cut(s) 105, 911
Bsp13I TCCGGA 1 cut(s) 1399
Bsp1407I TGTACA 1 cut(s) 1648
Bsp143I GATC 4 cut(s) 280, 638, 963, 1565
BspANI GGCC 5 cut(s) 5, 62, 96, 507, 1672
BspCNI CTCAG 3 cut(s) 813, 1110, 1561
BspEI TCCGGA 1 cut(s) 1399
BspHI TCATGA 1 cut(s) 913
BspLI GGNNCC 5 cut(s) 559, 579, 640, 757, 1060
BspMAI CTGCAG 1 cut(s) 905
BspOI GCTAGC 1 cut(s) 1577
BspPI GGATC 4 cut(s) 633, 646, 958, 1573
BspT107I GGYRCC 2 cut(s) 755, 1058
BsrDI GCAATG 2 cut(s) 175, 1605
BsrFI RCCGGY 1 cut(s) 1061
BsrGI TGTACA 1 cut(s) 1648
BsrI ACTGG 4 cut(s) 508, 541, 632, 1178
BssAI RCCGGY 1 cut(s) 1061
BssECI CCNNGG 1 cut(s) 1582
BssMI GATC 4 cut(s) 280, 638, 963, 1565
Bst2UI CCWGG 4 cut(s) 165, 643, 1136, 1584
Bst4CI ACNGT 4 cut(s) 380, 1496, 1658, 1759
BstAPI GCANNNNNTGC 1 cut(s) 1065
BstAUI TGTACA 1 cut(s) 1648
BstC8I GCNNGC 2 cut(s) 1273, 1575
BstDEI CTNAG 9 cut(s) 85, 187, 272, 395, 800, 1118, 1168, 1569, 1619
BstENI CCTNNNNNAGG 1 cut(s) 552
BstF5I GGATG 7 cut(s) 115, 120, 819, 823, 1009, 1131, 1690
BstKTI GATC 4 cut(s) 283, 641, 966, 1568
BstMAI GTCTC 3 cut(s) 951, 1234, 1401
BstMBI GATC 4 cut(s) 280, 638, 963, 1565
BstMWI GCNNNNNNNGC 2 cut(s) 1065, 1678
BstNI CCWGG 4 cut(s) 165, 643, 1136, 1584
BstSCI CCNGG 4 cut(s) 163, 641, 1134, 1582
BstSFI CTRYAG 1 cut(s) 901
BstV1I GCAGC 5 cut(s) 29, 130, 1052, 1555, 1673
BstX2I RGATCY 3 cut(s) 638, 963, 1565
BstYI RGATCY 3 cut(s) 638, 963, 1565
BsuRI GGCC 5 cut(s) 5, 62, 96, 507, 1672
BtsCI GGATG 7 cut(s) 115, 120, 819, 823, 1009, 1131, 1690
BtsI GCAGTG 1 cut(s) 958
BtsIMutI CAGTG 2 cut(s) 385, 958
Cac8I GCNNGC 2 cut(s) 1273, 1575
CaiI CAGNNNCTG 1 cut(s) 134
CciI TCATGA 1 cut(s) 913
Cfr10I RCCGGY 1 cut(s) 1061
Cfr13I GGNCC 3 cut(s) 558, 577, 1513
Csp6I GTAC 6 cut(s) 199, 329, 625, 694, 756, 1649
CviQI GTAC 6 cut(s) 199, 329, 625, 694, 756, 1649
DdeI CTNAG 9 cut(s) 85, 187, 272, 395, 800, 1118, 1168, 1569, 1619
DpnI GATC 4 cut(s) 282, 640, 965, 1567
DpnII GATC 4 cut(s) 280, 638, 963, 1565
DraI TTTAAA 2 cut(s) 1266, 1704
DraIII CACNNNGTG 1 cut(s) 654
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 2 cut(s) 103, 909
Eco147I AGGCCT 1 cut(s) 62
Eco24I GRGCYC 2 cut(s) 105, 911
Eco47I GGWCC 3 cut(s) 558, 577, 1513
Eco53kI GAGCTC 2 cut(s) 103, 909
Eco57I CTGAAG 3 cut(s) 643, 1171, 1632
Eco88I CYCGRG 1 cut(s) 1237
EcoICRI GAGCTC 2 cut(s) 103, 909
EcoNI CCTNNNNNAGG 1 cut(s) 552
EcoO109I RGGNCCY 1 cut(s) 558
EcoRII CCWGG 4 cut(s) 163, 641, 1134, 1582
EcoT22I ATGCAT 2 cut(s) 1540, 1628
EcoT38I GRGCYC 2 cut(s) 105, 911
FalI AAGNNNNNCTT 8 cut(s) 166, 198, 683, 715, 1151, 1183, 1169, 1201
FauNDI CATATG 1 cut(s) 1384
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 5 cut(s) 43, 119, 1066, 1544, 1662
FokI GGATG 7 cut(s) 122, 127, 826, 830, 1016, 1118, 1697
FriOI GRGCYC 2 cut(s) 105, 911
Fsp4HI GCNGC 5 cut(s) 43, 119, 1066, 1544, 1662
FspBI CTAG 6 cut(s) 360, 549, 582, 633, 1329, 1574
GluI GCNGC 5 cut(s) 43, 119, 1066, 1544, 1662
GsuI CTGGAG 1 cut(s) 285
HaeIII GGCC 5 cut(s) 5, 62, 96, 507, 1672
HapII CCGG 3 cut(s) 1062, 1400, 1682
HindIII AAGCTT 1 cut(s) 442
HpaII CCGG 3 cut(s) 1062, 1400, 1682
HphI GGTGA 1 cut(s) 806
Hpy166II GTNNAC 3 cut(s) 797, 828, 1649
Hpy8I GTNNAC 3 cut(s) 797, 828, 1649
HpyAV CCTTC 1 cut(s) 785
HpyCH4III ACNGT 4 cut(s) 380, 1496, 1658, 1759
HpyF10VI GCNNNNNNNGC 2 cut(s) 1065, 1678
HpyF3I CTNAG 9 cut(s) 85, 187, 272, 395, 800, 1118, 1168, 1569, 1619
Kpn2I TCCGGA 1 cut(s) 1399
KpnI GGTACC 1 cut(s) 759
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 4 cut(s) 280, 638, 963, 1565
LmnI GCTCC 2 cut(s) 18, 906
Lsp1109I GCAGC 5 cut(s) 29, 130, 1052, 1555, 1673
LweI GCATC 7 cut(s) 105, 140, 808, 1138, 1200, 1613, 1790
MaeI CTAG 6 cut(s) 360, 549, 582, 633, 1329, 1574
MaeIII GTNAC 5 cut(s) 176, 290, 683, 1496, 1819
MalI GATC 4 cut(s) 282, 640, 965, 1567
MboI GATC 4 cut(s) 280, 638, 963, 1565
MfeI CAATTG 1 cut(s) 511
MflI RGATCY 3 cut(s) 638, 963, 1565
MhlI GDGCHC 2 cut(s) 105, 911
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 9 cut(s) 259, 276, 385, 511, 541, 920, 1105, 1470, 1800
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 7 cut(s) 62, 511, 768, 979, 1358, 1464, 1802
MmeI TCCRAC 2 cut(s) 479, 1357
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 1540, 1628
MroI TCCGGA 1 cut(s) 1399
MroXI GAANNNNTTC 1 cut(s) 1184
MscI TGGCCA 1 cut(s) 5
MseI TTAA 8 cut(s) 159, 342, 492, 591, 782, 1265, 1431, 1703
MslI CAYNNNNRTG 1 cut(s) 1631
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 1062, 1400, 1682
MspR9I CCNGG 4 cut(s) 165, 643, 1136, 1584
MunI CAATTG 1 cut(s) 511
Mva1269I GAATGC 2 cut(s) 1626, 1713
MvaI CCWGG 4 cut(s) 165, 643, 1136, 1584
MwoI GCNNNNNNNGC 2 cut(s) 1065, 1678
NdeI CATATG 1 cut(s) 1384
NdeII GATC 4 cut(s) 280, 638, 963, 1565
NheI GCTAGC 1 cut(s) 1573
NlaIV GGNNCC 5 cut(s) 559, 579, 640, 757, 1060
NsiI ATGCAT 2 cut(s) 1540, 1628
PaeR7I CTCGAG 1 cut(s) 1237
PagI TCATGA 1 cut(s) 913
PceI AGGCCT 1 cut(s) 62
PctI GAATGC 2 cut(s) 1626, 1713
PdmI GAANNNNTTC 1 cut(s) 1184
PfeI GAWTC 6 cut(s) 153, 250, 1139, 1252, 1294, 1595
PflMI CCANNNNNTGG 1 cut(s) 935
PfoI TCCNGGA 1 cut(s) 1134
PkrI GCNGC 5 cut(s) 44, 120, 1067, 1545, 1663
PleI GAGTC 7 cut(s) 62, 511, 768, 979, 1357, 1463, 1801
PpsI GAGTC 7 cut(s) 62, 511, 768, 979, 1357, 1463, 1801
PpuMI RGGWCCY 1 cut(s) 558
PsiI TTATAA 2 cut(s) 1751, 1775
Psp124BI GAGCTC 2 cut(s) 105, 911
Psp5II RGGWCCY 1 cut(s) 558
Psp6I CCWGG 4 cut(s) 163, 641, 1134, 1582
PspGI CCWGG 4 cut(s) 163, 641, 1134, 1582
PspN4I GGNNCC 5 cut(s) 559, 579, 640, 757, 1060
PspPI GGNCC 3 cut(s) 558, 577, 1513
PspPPI RGGWCCY 1 cut(s) 558
PstI CTGCAG 1 cut(s) 905
PstNI CAGNNNCTG 1 cut(s) 134
PsuI RGATCY 3 cut(s) 638, 963, 1565
RsaI GTAC 6 cut(s) 200, 330, 626, 695, 757, 1650
RsaNI GTAC 6 cut(s) 199, 329, 625, 694, 756, 1649
RseI CAYNNNNRTG 1 cut(s) 1631
SacI GAGCTC 2 cut(s) 105, 911
SaqAI TTAA 8 cut(s) 159, 342, 492, 591, 782, 1265, 1431, 1703
SatI GCNGC 5 cut(s) 43, 119, 1066, 1544, 1662
Sau3AI GATC 4 cut(s) 280, 638, 963, 1565
Sau96I GGNCC 3 cut(s) 558, 577, 1513
SchI GAGTC 7 cut(s) 62, 511, 768, 979, 1358, 1464, 1802
ScrFI CCNGG 4 cut(s) 165, 643, 1136, 1584
SduI GDGCHC 2 cut(s) 105, 911
SfaNI GCATC 7 cut(s) 105, 140, 808, 1138, 1200, 1613, 1790
SfcI CTRYAG 1 cut(s) 901
Sfr274I CTCGAG 1 cut(s) 1237
SinI GGWCC 3 cut(s) 558, 577, 1513
SlaI CTCGAG 1 cut(s) 1237
SmiMI CAYNNNNRTG 1 cut(s) 1631
SmlI CTYRAG 5 cut(s) 104, 733, 968, 1209, 1237
SmoI CTYRAG 5 cut(s) 104, 733, 968, 1209, 1237
SpeI ACTAGT 2 cut(s) 359, 632
Sse9I AATT 9 cut(s) 259, 276, 385, 511, 541, 920, 1105, 1470, 1800
SseBI AGGCCT 1 cut(s) 62
SspI AATATT 1 cut(s) 1279
SspMI CTAG 6 cut(s) 360, 549, 582, 633, 1329, 1574
SstI GAGCTC 2 cut(s) 105, 911
StuI AGGCCT 1 cut(s) 62
StyD4I CCNGG 4 cut(s) 163, 641, 1134, 1582
TaaI ACNGT 4 cut(s) 380, 1496, 1658, 1759
TaqI TCGA 2 cut(s) 1238, 1287
TasI AATT 9 cut(s) 259, 276, 385, 511, 541, 920, 1105, 1470, 1800
TatI WGTACW 4 cut(s) 328, 624, 693, 1648
TfiI GAWTC 6 cut(s) 153, 250, 1139, 1252, 1294, 1595
Tru1I TTAA 8 cut(s) 159, 342, 492, 591, 782, 1265, 1431, 1703
Tru9I TTAA 8 cut(s) 159, 342, 492, 591, 782, 1265, 1431, 1703
TscAI CASTG 2 cut(s) 385, 958
TseI GCWGC 5 cut(s) 42, 118, 1065, 1543, 1661
TspRI CASTG 2 cut(s) 385, 958
Van91I CCANNNNNTGG 1 cut(s) 935
VpaK11BI GGWCC 3 cut(s) 558, 577, 1513
XagI CCTNNNNNAGG 1 cut(s) 552
XapI RAATTY 4 cut(s) 385, 541, 920, 1800
XhoI CTCGAG 1 cut(s) 1237
XmnI GAANNNNTTC 1 cut(s) 1184
XspI CTAG 6 cut(s) 360, 549, 582, 633, 1329, 1574
Zsp2I ATGCAT 2 cut(s) 1540, 1628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.