Rh2DG417300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001316
Physical Location & Seq
Reverse (-)
60607931 .. 60608800
870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG417300.1

Sequence Viewer

Length: 672 bp
ATGGACCCCAACCACATATACATTGTTGGCAACTCGGTCAAATTCGCTCACTTTGACAAGGTAACAAGAAGTCGAGATGATCAACAGCAAAGCGTCTTTCAGCTTCTGAGAGCTTCTTTGCCACAGAATGTTCTAACTGGAGAGCTGTTACACTTCATTGGCCTACAAACAAACTGTACAAGAGAGAGATTCTTATTCTATAGGCTCCATCCAGGGATCTCCAATGGAGATGAAATGTGCTCCCTAAGACTTCACTCCTTTGAAACATCAAAAATTCAAGGACTTGGTCTGGCAAATAAAATTCTCAACAAATCTAGAGACACTGCCTATACTTCAATTGTAAAACTGTGGAACAAGCATTGGCACAATAATTGGCTGTCGAGGATTACCTCAACGAGGCTGAGAGCTTTAGGCTGCTACTCTCTTTATTTGAATAAGAAGAAACCCCTACCAGTTGCAGACTTTGTGAATTTCAGCCGGGCTGCTGTTGAGCATGCCAATGATCAAAAAACTATGGCTAATGATCCTATCTCTCAAGGTCGAATCATTAGTTATGAGGAAGTGGATGAGGAGCTAAACAATGCATGGCCTCAGTTTATGATACTCTGGTTTGATTGCTGTGTTGATGATATAGCCTACTACGGCAACATGTTTCATTTCTCTACACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

223

Amino Acids

25.87

Weight (kDa)

8.26

Isoelectric Point (pI)

33.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 224, 518
AcsI RAATTY 4 cut(s) 41, 273, 300, 469
AfaI GTAC 1 cut(s) 178
AfiI CCNNNNNNNGG 1 cut(s) 396
AflIII ACRYGT 1 cut(s) 648
AgsI TTSAA 4 cut(s) 263, 278, 336, 433
AjnI CCWGG 1 cut(s) 211
AluBI AGCT 5 cut(s) 103, 113, 145, 407, 574
AluI AGCT 5 cut(s) 103, 113, 145, 407, 574
Alw21I GWGCWC 1 cut(s) 242
Alw26I GTCTC 1 cut(s) 312
AlwI GGATC 2 cut(s) 224, 518
AlwNI CAGNNNCTG 1 cut(s) 106
AoxI GGCC 2 cut(s) 160, 587
ApeKI GCWGC 2 cut(s) 414, 482
ApoI RAATTY 4 cut(s) 41, 273, 300, 469
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 479
AvaII GGWCC 1 cut(s) 4
BaeI ACNNNNGTAYC 2 cut(s) 593, 626
Bbv12I GWGCWC 1 cut(s) 242
BbvI GCAGC 2 cut(s) 401, 469
BccI CCATC 1 cut(s) 216
BceAI ACGGC 1 cut(s) 658
BciT130I CCWGG 1 cut(s) 213
BclI TGATCA 2 cut(s) 79, 502
BcnI CCSGG 1 cut(s) 479
BcoDI GTCTC 1 cut(s) 312
BfaI CTAG 1 cut(s) 315
BfmI CTRYAG 1 cut(s) 199
BisI GCNGC 2 cut(s) 415, 483
BlsI GCNGC 2 cut(s) 416, 484
Bme1390I CCNGG 2 cut(s) 213, 479
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 6, 206
BmrFI CCNGG 2 cut(s) 213, 479
BpmI CTGGAG 1 cut(s) 159
BpuEI CTTGAG 1 cut(s) 519
BpuMI CCSGG 1 cut(s) 479
BsaJI CCNNGG 1 cut(s) 212
Bsc4I CCNNNNNNNGG 1 cut(s) 396
Bse1I ACTGG 2 cut(s) 142, 452
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 1 cut(s) 212
BseGI GGATG 2 cut(s) 208, 571
BseLI CCNNNNNNNGG 1 cut(s) 396
BseMII CTCAG 3 cut(s) 98, 392, 605
BseNI ACTGG 2 cut(s) 142, 452
BseRI GAGGAG 1 cut(s) 584
BseXI GCAGC 2 cut(s) 401, 469
BshFI GGCC 2 cut(s) 162, 589
BsiHKAI GWGCWC 1 cut(s) 242
BsiSI CCGG 1 cut(s) 478
BslI CCNNNNNNNGG 1 cut(s) 396
BsmAI GTCTC 1 cut(s) 312
BsnI GGCC 2 cut(s) 162, 589
Bsp1286I GDGCHC 1 cut(s) 242
Bsp1407I TGTACA 1 cut(s) 176
Bsp143I GATC 4 cut(s) 79, 216, 502, 523
BspANI GGCC 2 cut(s) 162, 589
BspCNI CTCAG 3 cut(s) 99, 393, 604
BspLI GGNNCC 2 cut(s) 6, 206
BspPI GGATC 2 cut(s) 224, 518
BsrGI TGTACA 1 cut(s) 176
BsrI ACTGG 2 cut(s) 142, 452
BssECI CCNNGG 1 cut(s) 212
BssMI GATC 4 cut(s) 79, 216, 502, 523
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 2 cut(s) 176, 348
BstAUI TGTACA 1 cut(s) 176
BstC8I GCNNGC 1 cut(s) 495
BstDEI CTNAG 4 cut(s) 107, 245, 401, 591
BstENI CCTNNNNNAGG 1 cut(s) 394
BstF5I GGATG 2 cut(s) 208, 571
BstKTI GATC 4 cut(s) 82, 219, 505, 526
BstMAI GTCTC 1 cut(s) 312
BstMBI GATC 4 cut(s) 79, 216, 502, 523
BstNI CCWGG 1 cut(s) 213
BstNSI RCATGY 2 cut(s) 497, 652
BstSCI CCNGG 2 cut(s) 211, 477
BstSFI CTRYAG 1 cut(s) 199
BstV1I GCAGC 2 cut(s) 401, 469
BstX2I RGATCY 1 cut(s) 216
BstYI RGATCY 1 cut(s) 216
BsuRI GGCC 2 cut(s) 162, 589
BtsCI GGATG 2 cut(s) 208, 571
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 1 cut(s) 321
Cac8I GCNNGC 1 cut(s) 495
CaiI CAGNNNCTG 1 cut(s) 106
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 1 cut(s) 82
Csp6I GTAC 1 cut(s) 177
CviAII CATG 3 cut(s) 494, 585, 649
CviQI GTAC 1 cut(s) 177
DdeI CTNAG 4 cut(s) 107, 245, 401, 591
DpnI GATC 4 cut(s) 81, 218, 504, 525
DpnII GATC 4 cut(s) 79, 216, 502, 523
Eco47I GGWCC 1 cut(s) 4
EcoNI CCTNNNNNAGG 1 cut(s) 394
EcoRII CCWGG 1 cut(s) 211
EcoT22I ATGCAT 1 cut(s) 586
FaeI CATG 3 cut(s) 497, 588, 652
FatI CATG 3 cut(s) 493, 584, 648
FbaI TGATCA 2 cut(s) 79, 502
Fnu4HI GCNGC 2 cut(s) 415, 483
FokI GGATG 2 cut(s) 195, 578
Fsp4HI GCNGC 2 cut(s) 415, 483
FspBI CTAG 1 cut(s) 315
GluI GCNGC 2 cut(s) 415, 483
GsuI CTGGAG 1 cut(s) 159
HaeIII GGCC 2 cut(s) 162, 589
HapII CCGG 1 cut(s) 478
HgaI GACGC 1 cut(s) 82
Hin1II CATG 3 cut(s) 497, 588, 652
HinfI GANTC 2 cut(s) 189, 543
HpaII CCGG 1 cut(s) 478
Hpy188I TCNGA 1 cut(s) 108
Hpy188III TCNNGA 2 cut(s) 74, 315
HpyCH4III ACNGT 2 cut(s) 176, 348
HpyCH4V TGCA 2 cut(s) 458, 584
HpyF3I CTNAG 4 cut(s) 107, 245, 401, 591
Hsp92II CATG 3 cut(s) 497, 588, 652
Ksp22I TGATCA 2 cut(s) 79, 502
Kzo9I GATC 4 cut(s) 79, 216, 502, 523
LmnI GCTCC 3 cut(s) 210, 245, 571
LpnPI CCDG 7 cut(s) 123, 198, 225, 275, 465, 491, 592
Lsp1109I GCAGC 2 cut(s) 401, 469
MaeI CTAG 1 cut(s) 315
MaeIII GTNAC 2 cut(s) 61, 147
MalI GATC 4 cut(s) 81, 218, 504, 525
MboI GATC 4 cut(s) 79, 216, 502, 523
MboII GAAGA 1 cut(s) 451
MfeI CAATTG 1 cut(s) 336
MflI RGATCY 1 cut(s) 216
MhlI GDGCHC 1 cut(s) 242
MluCI AATT 6 cut(s) 41, 273, 300, 336, 370, 469
MnlI CCTC 6 cut(s) 375, 390, 400, 550, 562, 600
Mph1103I ATGCAT 1 cut(s) 586
MslI CAYNNNNRTG 1 cut(s) 498
MspI CCGG 1 cut(s) 478
MspR9I CCNGG 2 cut(s) 213, 479
MunI CAATTG 1 cut(s) 336
MvaI CCWGG 1 cut(s) 213
NciI CCSGG 1 cut(s) 479
NdeII GATC 4 cut(s) 79, 216, 502, 523
NlaIII CATG 3 cut(s) 497, 588, 652
NlaIV GGNNCC 2 cut(s) 6, 206
NsiI ATGCAT 1 cut(s) 586
NspI RCATGY 2 cut(s) 497, 652
PaeI GCATGC 1 cut(s) 497
PciI ACATGT 1 cut(s) 648
PfeI GAWTC 2 cut(s) 189, 543
PflFI GACNNNGTC 1 cut(s) 285
PkrI GCNGC 2 cut(s) 416, 484
PscI ACATGT 1 cut(s) 648
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 2 cut(s) 6, 206
PspPI GGNCC 1 cut(s) 4
PstNI CAGNNNCTG 1 cut(s) 106
PsuI RGATCY 1 cut(s) 216
PsyI GACNNNGTC 1 cut(s) 285
RsaI GTAC 1 cut(s) 178
RsaNI GTAC 1 cut(s) 177
RseI CAYNNNNRTG 1 cut(s) 498
SatI GCNGC 2 cut(s) 415, 483
Sau3AI GATC 4 cut(s) 79, 216, 502, 523
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 2 cut(s) 213, 479
SduI GDGCHC 1 cut(s) 242
SetI ASST 8 cut(s) 63, 105, 115, 147, 392, 409, 541, 576
SfcI CTRYAG 1 cut(s) 199
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 1 cut(s) 498
SmlI CTYRAG 1 cut(s) 534
SmoI CTYRAG 1 cut(s) 534
SphI GCATGC 1 cut(s) 497
Sse9I AATT 6 cut(s) 41, 273, 300, 336, 370, 469
SspMI CTAG 1 cut(s) 315
StyD4I CCNGG 2 cut(s) 211, 477
TaaI ACNGT 2 cut(s) 176, 348
TaqI TCGA 3 cut(s) 73, 380, 541
TaqII GACCGA 1 cut(s) 25
TasI AATT 6 cut(s) 41, 273, 300, 336, 370, 469
TatI WGTACW 1 cut(s) 176
TfiI GAWTC 2 cut(s) 189, 543
TscAI CASTG 1 cut(s) 328
TseI GCWGC 2 cut(s) 414, 482
TspDTI ATGAA 3 cut(s) 145, 246, 644
TspRI CASTG 1 cut(s) 328
Tth111I GACNNNGTC 1 cut(s) 285
VpaK11BI GGWCC 1 cut(s) 4
XagI CCTNNNNNAGG 1 cut(s) 394
XapI RAATTY 4 cut(s) 41, 273, 300, 469
XbaI TCTAGA 1 cut(s) 314
XceI RCATGY 2 cut(s) 497, 652
XspI CTAG 1 cut(s) 315
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.