Rroxscaffold_2G00124430

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010625
Physical Location & Seq
Forward (+)
58717658 .. 58724083
6426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00124430.1

Sequence Viewer

Length: 552 bp
ATGGCCGCCAACCGCGCTGCCGTATCTTCTCCGCGGCAGCGAAGAAGGACCATGTCCGCTCTCGAAGCCCGAATCTCCTCGTTGCCGCTCTCGCCACCCAAACCTCCTCCGTCTCCTAGTAACATAAATATTACAATATGGGTTAGCAGTATATTGTTTGATACATATGCTGATATTGCATCTAAGGGCACAGCTGATCAGCTATATAGTATTATTTCTGGAATGCATCAATTTAGTAATTGGATTTTAATACCCAACTTAGCAGTTATGCTTAGTGGAGCACGATCTAGTATACTTGAGGAGATGAAGTTGTTAAAAGAACTGCAGGATCATTCTGTTTACGTGCACGCTAACTCAAGTTTGCTTTTCATTGTCTCGGGTTTCTCGTTGACTCATGTGGATGAGCTTATTGATGAACTTCGACAAAGGACTACTCATGCGACATTGCCATGCCACGAATCAAGAAAAGGGAAGAATATCAAGGAAGCTGATCGAAGTCTTAAGGTAACCCTTTCGGAAGAACCAGCTTTTCGGAGATGTAATTTAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

20.34

Weight (kDa)

9.26

Isoelectric Point (pI)

66.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 59, 88
AccI GTMKAC 1 cut(s) 292
AccII CGCG 2 cut(s) 15, 34
AciI CCGC 6 cut(s) 6, 13, 32, 34, 57, 86
AclWI GGATC 1 cut(s) 336
AcoI YGGCCR 1 cut(s) 3
AflII CTTAAG 1 cut(s) 500
AluBI AGCT 5 cut(s) 194, 202, 406, 488, 527
AluI AGCT 5 cut(s) 194, 202, 406, 488, 527
Alw21I GWGCWC 2 cut(s) 283, 348
Alw26I GTCTC 2 cut(s) 117, 379
Alw44I GTGCAC 1 cut(s) 344
AlwI GGATC 1 cut(s) 336
Ama87I CYCGRG 1 cut(s) 376
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 344
ApeKI GCWGC 2 cut(s) 17, 37
AspLEI GCGC 1 cut(s) 17
AspS9I GGNCC 1 cut(s) 48
AvaI CYCGRG 1 cut(s) 376
AvaII GGWCC 1 cut(s) 48
BaeGI GKGCMC 2 cut(s) 191, 348
Bbv12I GWGCWC 2 cut(s) 283, 348
BbvI GCAGC 2 cut(s) 4, 49
BceAI ACGGC 1 cut(s) 5
BclI TGATCA 1 cut(s) 196
BcoDI GTCTC 2 cut(s) 117, 379
BfaI CTAG 2 cut(s) 117, 288
BfmI CTRYAG 1 cut(s) 323
BfrI CTTAAG 1 cut(s) 500
BisI GCNGC 5 cut(s) 6, 18, 35, 38, 86
BlsI GCNGC 5 cut(s) 7, 19, 36, 39, 87
Bme18I GGWCC 1 cut(s) 48
BmeT110I CYCGRG 1 cut(s) 376
BmgT120I GGNCC 1 cut(s) 48
BmsI GCATC 2 cut(s) 188, 235
BpuEI CTTGAG 2 cut(s) 317, 340
BsaAI YACGTR 1 cut(s) 343
BsaJI CCNNGG 1 cut(s) 32
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bse3DI GCAATG 1 cut(s) 443
BseDI CCNNGG 1 cut(s) 32
BseGI GGATG 1 cut(s) 406
BseMI GCAATG 1 cut(s) 443
BseRI GAGGAG 3 cut(s) 67, 96, 314
BseSI GKGCMC 2 cut(s) 191, 348
BseXI GCAGC 2 cut(s) 4, 49
Bsh1236I CGCG 2 cut(s) 15, 34
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 2 cut(s) 283, 348
BsiHKCI CYCGRG 1 cut(s) 376
BsmAI GTCTC 2 cut(s) 117, 379
BsmBI CGTCTC 1 cut(s) 117
BsmI GAATGC 1 cut(s) 228
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 376
Bsp1286I GDGCHC 3 cut(s) 191, 283, 348
Bsp143I GATC 4 cut(s) 196, 284, 328, 490
BspACI CCGC 6 cut(s) 6, 13, 32, 34, 57, 86
BspANI GGCC 1 cut(s) 5
BspFNI CGCG 2 cut(s) 15, 34
BspMAI CTGCAG 1 cut(s) 327
BspPI GGATC 1 cut(s) 336
BspTI CTTAAG 1 cut(s) 500
BsrBI CCGCTC 2 cut(s) 59, 88
BsrDI GCAATG 1 cut(s) 443
BssECI CCNNGG 1 cut(s) 32
BssMI GATC 4 cut(s) 196, 284, 328, 490
BssNAI GTATAC 1 cut(s) 293
Bst1107I GTATAC 1 cut(s) 293
BstAFI CTTAAG 1 cut(s) 500
BstBAI YACGTR 1 cut(s) 343
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 3 cut(s) 183, 259, 272
BstDSI CCRYGG 1 cut(s) 32
BstEII GGTNACC 1 cut(s) 505
BstF5I GGATG 1 cut(s) 406
BstFNI CGCG 2 cut(s) 15, 34
BstHHI GCGC 1 cut(s) 17
BstKTI GATC 4 cut(s) 199, 287, 331, 493
BstMAI GTCTC 2 cut(s) 117, 379
BstMBI GATC 4 cut(s) 196, 284, 328, 490
BstMWI GCNNNNNNNGC 4 cut(s) 14, 65, 91, 176
BstPI GGTNACC 1 cut(s) 505
BstSFI CTRYAG 1 cut(s) 323
BstSLI GKGCMC 2 cut(s) 191, 348
BstUI CGCG 2 cut(s) 15, 34
BstV1I GCAGC 2 cut(s) 4, 49
BstZ17I GTATAC 1 cut(s) 293
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 32
BtsCI GGATG 1 cut(s) 406
Cac8I GCNNGC 1 cut(s) 348
CfoI GCGC 1 cut(s) 17
Cfr13I GGNCC 1 cut(s) 48
Cfr42I CCGCGG 1 cut(s) 35
CviAII CATG 4 cut(s) 52, 395, 437, 450
CviJI RGCY 7 cut(s) 5, 68, 194, 202, 406, 488, 527
CviKI_1 RGCY 7 cut(s) 5, 68, 194, 202, 406, 488, 527
DdeI CTNAG 3 cut(s) 183, 259, 272
DpnI GATC 4 cut(s) 198, 286, 330, 492
DpnII GATC 4 cut(s) 196, 284, 328, 490
EaeI YGGCCR 1 cut(s) 3
Eco47I GGWCC 1 cut(s) 48
Eco88I CYCGRG 1 cut(s) 376
Eco91I GGTNACC 1 cut(s) 505
EcoO65I GGTNACC 1 cut(s) 505
EcoT22I ATGCAT 1 cut(s) 228
Esp3I CGTCTC 1 cut(s) 117
FaeI CATG 4 cut(s) 55, 398, 440, 453
FatI CATG 4 cut(s) 51, 394, 436, 449
FauNDI CATATG 1 cut(s) 166
FbaI TGATCA 1 cut(s) 196
FblI GTMKAC 1 cut(s) 292
Fnu4HI GCNGC 5 cut(s) 6, 18, 35, 38, 86
FokI GGATG 1 cut(s) 413
Fsp4HI GCNGC 5 cut(s) 6, 18, 35, 38, 86
FspBI CTAG 2 cut(s) 117, 288
GlaI GCGC 1 cut(s) 16
GluI GCNGC 5 cut(s) 6, 18, 35, 38, 86
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 17
Hin1II CATG 4 cut(s) 55, 398, 440, 453
Hin6I GCGC 1 cut(s) 15
HinP1I GCGC 1 cut(s) 15
HincII GTYRAC 1 cut(s) 390
HindII GTYRAC 1 cut(s) 390
HinfI GANTC 3 cut(s) 72, 391, 458
Hpy166II GTNNAC 4 cut(s) 293, 340, 346, 390
Hpy188I TCNGA 2 cut(s) 517, 534
Hpy188III TCNNGA 3 cut(s) 62, 219, 462
Hpy8I GTNNAC 4 cut(s) 293, 340, 346, 390
HpyAV CCTTC 1 cut(s) 39
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 4 cut(s) 179, 226, 325, 346
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 65, 91, 176
HpyF3I CTNAG 3 cut(s) 183, 259, 272
HpySE526I ACGT 1 cut(s) 342
Hsp92II CATG 4 cut(s) 55, 398, 440, 453
HspAI GCGC 1 cut(s) 15
Ksp22I TGATCA 1 cut(s) 196
KspI CCGCGG 1 cut(s) 35
Kzo9I GATC 4 cut(s) 196, 284, 328, 490
LmnI GCTCC 1 cut(s) 278
LpnPI CCDG 3 cut(s) 204, 311, 537
Lsp1109I GCAGC 2 cut(s) 4, 49
LweI GCATC 2 cut(s) 188, 235
MaeI CTAG 2 cut(s) 117, 288
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 2 cut(s) 119, 505
MalI GATC 4 cut(s) 198, 286, 330, 492
MbiI CCGCTC 2 cut(s) 59, 88
MboI GATC 4 cut(s) 196, 284, 328, 490
MboII GAAGA 4 cut(s) 18, 54, 484, 530
MhlI GDGCHC 3 cut(s) 191, 283, 348
MluCI AATT 3 cut(s) 230, 238, 541
MlyI GAGTC 1 cut(s) 385
MnlI CCTC 4 cut(s) 88, 114, 117, 292
Mph1103I ATGCAT 1 cut(s) 228
MseI TTAA 3 cut(s) 248, 314, 501
MslI CAYNNNNRTG 2 cut(s) 399, 448
MspA1I CMGCKG 2 cut(s) 34, 194
MspCI CTTAAG 1 cut(s) 500
Mva1269I GAATGC 1 cut(s) 228
MvnI CGCG 2 cut(s) 15, 34
MwoI GCNNNNNNNGC 4 cut(s) 14, 65, 91, 176
NdeI CATATG 1 cut(s) 166
NdeII GATC 4 cut(s) 196, 284, 328, 490
NlaIII CATG 4 cut(s) 55, 398, 440, 453
NsiI ATGCAT 1 cut(s) 228
PcsI WCGNNNNNNNCGW 1 cut(s) 383
PctI GAATGC 1 cut(s) 228
PfeI GAWTC 2 cut(s) 72, 458
PflFI GACNNNGTC 1 cut(s) 52
PkrI GCNGC 5 cut(s) 7, 19, 36, 39, 87
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
Ppu21I YACGTR 1 cut(s) 343
PspEI GGTNACC 1 cut(s) 505
PspPI GGNCC 1 cut(s) 48
PstI CTGCAG 1 cut(s) 327
PsyI GACNNNGTC 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 194
RseI CAYNNNNRTG 2 cut(s) 399, 448
SacII CCGCGG 1 cut(s) 35
SaqAI TTAA 3 cut(s) 248, 314, 501
SatI GCNGC 5 cut(s) 6, 18, 35, 38, 86
Sau3AI GATC 4 cut(s) 196, 284, 328, 490
Sau96I GGNCC 1 cut(s) 48
SchI GAGTC 1 cut(s) 385
SduI GDGCHC 3 cut(s) 191, 283, 348
SetI ASST 8 cut(s) 106, 196, 204, 345, 408, 490, 507, 529
SfaNI GCATC 2 cut(s) 188, 235
SfcI CTRYAG 1 cut(s) 323
Sfr303I CCGCGG 1 cut(s) 35
SgrBI CCGCGG 1 cut(s) 35
SinI GGWCC 1 cut(s) 48
SmiMI CAYNNNNRTG 2 cut(s) 399, 448
SmlI CTYRAG 3 cut(s) 296, 355, 500
SmoI CTYRAG 3 cut(s) 296, 355, 500
Sse9I AATT 3 cut(s) 230, 238, 541
SsiI CCGC 6 cut(s) 6, 13, 32, 34, 57, 86
SspI AATATT 1 cut(s) 130
SspMI CTAG 2 cut(s) 117, 288
TaiI ACGT 1 cut(s) 345
TaqI TCGA 3 cut(s) 63, 421, 493
TasI AATT 3 cut(s) 230, 238, 541
TauI GCSGC 3 cut(s) 8, 37, 88
TfiI GAWTC 2 cut(s) 72, 458
Tru1I TTAA 3 cut(s) 248, 314, 501
Tru9I TTAA 3 cut(s) 248, 314, 501
TseI GCWGC 2 cut(s) 17, 37
TspDTI ATGAA 3 cut(s) 320, 358, 429
TspGWI ACGGA 1 cut(s) 99
Tth111I GACNNNGTC 1 cut(s) 52
Vha464I CTTAAG 1 cut(s) 500
VneI GTGCAC 1 cut(s) 344
VpaK11BI GGWCC 1 cut(s) 48
XmiI GTMKAC 1 cut(s) 292
XspI CTAG 2 cut(s) 117, 288
Zsp2I ATGCAT 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.