Rroxscaffold_4G00312070

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010625
Physical Location & Seq
Reverse (-)
34773720 .. 34780890
7171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00312070.1

Sequence Viewer

Length: 597 bp
ATGGCCGCCAACCGCGCTGCCGTATCTTCTCCGCGGCAGCGAAGAAGGACCATGTCCGCTCTCGAAGCCCGAATCTCCCTCGTTGCCGCTCTCGCCACCCAAACCTCCTCCATTACAATATGGGTTAGCAGTATATTGTTTGATACATATGCTGATATTGCATCTAAGGGCACAGCTGATCAGCTATATAGTATTATTTCTGGAATGCATCAATTTAGTAATTGGATTTTAATACCCAACTTAGCAGTTATGCTTAGTGGAGCACGATCTAGTATACTTGAGGAGATGAAGTTGTTAAAAGAACTGCAGGATCATTCTGTTTACGTGCACGCTAACTCAAGTTTGCTTTTCATTGTCTCGGGTTTCTCGTTGACTCATGTGGATGAGCTTATTGATGAACTTCGACAAAGGACTACTCATGCGACATTGCCATGCCACGAATCAAGAAAAGATTCCACCTATCTATATGTTCTAATTGCATCTGGTCCTTTCTACTTATGTTTTATGCTACCTTCATACTGCTATCCCAGGTCTCTAAGAGGATCTATTGCAGCAGTCATGCAGCACTCAAGTGAGGACTATCTTGATCAGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.1

Weight (kDa)

6.64

Isoelectric Point (pI)

50.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 59, 89
AccI GTMKAC 1 cut(s) 274
AccII CGCG 2 cut(s) 15, 34
AciI CCGC 6 cut(s) 6, 13, 32, 34, 57, 87
AclWI GGATC 2 cut(s) 318, 550
AcoI YGGCCR 1 cut(s) 3
AjnI CCWGG 1 cut(s) 527
AleI CACNNNNGTG 1 cut(s) 570
AluBI AGCT 3 cut(s) 176, 184, 388
AluI AGCT 3 cut(s) 176, 184, 388
Alw21I GWGCWC 2 cut(s) 265, 330
Alw26I GTCTC 2 cut(s) 361, 537
Alw44I GTGCAC 1 cut(s) 326
AlwI GGATC 2 cut(s) 318, 550
Ama87I CYCGRG 1 cut(s) 358
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 326
ApeKI GCWGC 4 cut(s) 17, 37, 551, 562
Asp700I GAANNNNTTC 1 cut(s) 451
AspLEI GCGC 1 cut(s) 17
AspS9I GGNCC 2 cut(s) 48, 485
AvaI CYCGRG 1 cut(s) 358
AvaII GGWCC 2 cut(s) 48, 485
BaeGI GKGCMC 2 cut(s) 173, 330
Bbv12I GWGCWC 2 cut(s) 265, 330
BbvI GCAGC 4 cut(s) 4, 49, 563, 574
BceAI ACGGC 1 cut(s) 5
BciT130I CCWGG 1 cut(s) 529
BclI TGATCA 2 cut(s) 178, 586
BcoDI GTCTC 2 cut(s) 361, 537
BfaI CTAG 2 cut(s) 270, 595
BfmI CTRYAG 1 cut(s) 305
BisI GCNGC 7 cut(s) 6, 18, 35, 38, 87, 552, 563
BlsI GCNGC 7 cut(s) 7, 19, 36, 39, 88, 553, 564
Bme1390I CCNGG 1 cut(s) 529
Bme18I GGWCC 2 cut(s) 48, 485
BmeT110I CYCGRG 1 cut(s) 358
BmgT120I GGNCC 2 cut(s) 48, 485
BmrFI CCNGG 1 cut(s) 529
BmsI GCATC 3 cut(s) 170, 217, 488
BpuEI CTTGAG 3 cut(s) 299, 322, 553
BsaAI YACGTR 1 cut(s) 325
BsaI GGTCTC 1 cut(s) 537
BsaJI CCNNGG 2 cut(s) 32, 527
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bse3DI GCAATG 1 cut(s) 425
BseBI CCWGG 1 cut(s) 529
BseDI CCNNGG 2 cut(s) 32, 527
BseGI GGATG 1 cut(s) 388
BseMI GCAATG 1 cut(s) 425
BseRI GAGGAG 2 cut(s) 97, 296
BseSI GKGCMC 2 cut(s) 173, 330
BseXI GCAGC 4 cut(s) 4, 49, 563, 574
Bsh1236I CGCG 2 cut(s) 15, 34
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 2 cut(s) 265, 330
BsiHKCI CYCGRG 1 cut(s) 358
BsmAI GTCTC 2 cut(s) 361, 537
BsmI GAATGC 1 cut(s) 210
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 537
BsoBI CYCGRG 1 cut(s) 358
Bsp1286I GDGCHC 3 cut(s) 173, 265, 330
Bsp143I GATC 5 cut(s) 178, 266, 310, 542, 586
BspACI CCGC 6 cut(s) 6, 13, 32, 34, 57, 87
BspANI GGCC 1 cut(s) 5
BspFNI CGCG 2 cut(s) 15, 34
BspMAI CTGCAG 1 cut(s) 309
BspPI GGATC 2 cut(s) 318, 550
BspTNI GGTCTC 1 cut(s) 537
BsrBI CCGCTC 2 cut(s) 59, 89
BsrDI GCAATG 1 cut(s) 425
BssECI CCNNGG 2 cut(s) 32, 527
BssMI GATC 5 cut(s) 178, 266, 310, 542, 586
BssNAI GTATAC 1 cut(s) 275
Bst1107I GTATAC 1 cut(s) 275
Bst2UI CCWGG 1 cut(s) 529
BstBAI YACGTR 1 cut(s) 325
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 4 cut(s) 165, 241, 254, 536
BstDSI CCRYGG 1 cut(s) 32
BstF5I GGATG 1 cut(s) 388
BstFNI CGCG 2 cut(s) 15, 34
BstHHI GCGC 1 cut(s) 17
BstKTI GATC 5 cut(s) 181, 269, 313, 545, 589
BstMAI GTCTC 2 cut(s) 361, 537
BstMBI GATC 5 cut(s) 178, 266, 310, 542, 586
BstMWI GCNNNNNNNGC 4 cut(s) 14, 65, 92, 158
BstNI CCWGG 1 cut(s) 529
BstSCI CCNGG 1 cut(s) 527
BstSFI CTRYAG 1 cut(s) 305
BstSLI GKGCMC 2 cut(s) 173, 330
BstUI CGCG 2 cut(s) 15, 34
BstV1I GCAGC 4 cut(s) 4, 49, 563, 574
BstX2I RGATCY 1 cut(s) 542
BstYI RGATCY 1 cut(s) 542
BstZ17I GTATAC 1 cut(s) 275
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 32
BtsCI GGATG 1 cut(s) 388
Cac8I GCNNGC 1 cut(s) 330
CfoI GCGC 1 cut(s) 17
Cfr13I GGNCC 2 cut(s) 48, 485
Cfr42I CCGCGG 1 cut(s) 35
CviAII CATG 5 cut(s) 52, 377, 419, 432, 559
CviJI RGCY 5 cut(s) 5, 68, 176, 184, 388
CviKI_1 RGCY 5 cut(s) 5, 68, 176, 184, 388
DdeI CTNAG 4 cut(s) 165, 241, 254, 536
DpnI GATC 5 cut(s) 180, 268, 312, 544, 588
DpnII GATC 5 cut(s) 178, 266, 310, 542, 586
EaeI YGGCCR 1 cut(s) 3
Eco31I GGTCTC 1 cut(s) 537
Eco47I GGWCC 2 cut(s) 48, 485
Eco88I CYCGRG 1 cut(s) 358
EcoRII CCWGG 1 cut(s) 527
EcoT22I ATGCAT 1 cut(s) 210
FaeI CATG 5 cut(s) 55, 380, 422, 435, 562
FatI CATG 5 cut(s) 51, 376, 418, 431, 558
FauNDI CATATG 1 cut(s) 148
FbaI TGATCA 2 cut(s) 178, 586
FblI GTMKAC 1 cut(s) 274
Fnu4HI GCNGC 7 cut(s) 6, 18, 35, 38, 87, 552, 563
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 7 cut(s) 6, 18, 35, 38, 87, 552, 563
FspBI CTAG 2 cut(s) 270, 595
GlaI GCGC 1 cut(s) 16
GluI GCNGC 7 cut(s) 6, 18, 35, 38, 87, 552, 563
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 17
Hin1II CATG 5 cut(s) 55, 380, 422, 435, 562
Hin6I GCGC 1 cut(s) 15
HinP1I GCGC 1 cut(s) 15
HincII GTYRAC 1 cut(s) 372
HindII GTYRAC 1 cut(s) 372
HinfI GANTC 4 cut(s) 72, 373, 440, 452
Hpy166II GTNNAC 4 cut(s) 275, 322, 328, 372
Hpy188I TCNGA 1 cut(s) 591
Hpy188III TCNNGA 4 cut(s) 62, 201, 444, 584
Hpy8I GTNNAC 4 cut(s) 275, 322, 328, 372
HpyAV CCTTC 2 cut(s) 39, 522
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 7 cut(s) 161, 208, 307, 328, 479, 551, 562
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 65, 92, 158
HpyF3I CTNAG 4 cut(s) 165, 241, 254, 536
HpySE526I ACGT 1 cut(s) 324
Hsp92II CATG 5 cut(s) 55, 380, 422, 435, 562
HspAI GCGC 1 cut(s) 15
Ksp22I TGATCA 2 cut(s) 178, 586
KspI CCGCGG 1 cut(s) 35
Kzo9I GATC 5 cut(s) 178, 266, 310, 542, 586
LmnI GCTCC 1 cut(s) 260
LpnPI CCDG 5 cut(s) 186, 293, 468, 514, 541
Lsp1109I GCAGC 4 cut(s) 4, 49, 563, 574
LweI GCATC 3 cut(s) 170, 217, 488
MaeI CTAG 2 cut(s) 270, 595
MaeII ACGT 1 cut(s) 324
MalI GATC 5 cut(s) 180, 268, 312, 544, 588
MbiI CCGCTC 2 cut(s) 59, 89
MboI GATC 5 cut(s) 178, 266, 310, 542, 586
MboII GAAGA 2 cut(s) 18, 54
MflI RGATCY 1 cut(s) 542
MhlI GDGCHC 3 cut(s) 173, 265, 330
MluCI AATT 3 cut(s) 212, 220, 474
MlyI GAGTC 1 cut(s) 367
MnlI CCTC 6 cut(s) 89, 115, 118, 274, 533, 568
Mph1103I ATGCAT 1 cut(s) 210
MroXI GAANNNNTTC 1 cut(s) 451
MseI TTAA 2 cut(s) 230, 296
MslI CAYNNNNRTG 3 cut(s) 381, 430, 570
MspA1I CMGCKG 2 cut(s) 34, 176
MspR9I CCNGG 1 cut(s) 529
Mva1269I GAATGC 1 cut(s) 210
MvaI CCWGG 1 cut(s) 529
MvnI CGCG 2 cut(s) 15, 34
MwoI GCNNNNNNNGC 4 cut(s) 14, 65, 92, 158
NdeI CATATG 1 cut(s) 148
NdeII GATC 5 cut(s) 178, 266, 310, 542, 586
NlaIII CATG 5 cut(s) 55, 380, 422, 435, 562
NsiI ATGCAT 1 cut(s) 210
OliI CACNNNNGTG 1 cut(s) 570
PcsI WCGNNNNNNNCGW 1 cut(s) 365
PctI GAATGC 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 451
PfeI GAWTC 3 cut(s) 72, 440, 452
PflFI GACNNNGTC 1 cut(s) 52
PkrI GCNGC 7 cut(s) 7, 19, 36, 39, 88, 553, 564
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
Ppu21I YACGTR 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 527
PspGI CCWGG 1 cut(s) 527
PspPI GGNCC 2 cut(s) 48, 485
PstI CTGCAG 1 cut(s) 309
PsuI RGATCY 1 cut(s) 542
PsyI GACNNNGTC 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 176
RseI CAYNNNNRTG 3 cut(s) 381, 430, 570
SacII CCGCGG 1 cut(s) 35
SaqAI TTAA 2 cut(s) 230, 296
SatI GCNGC 7 cut(s) 6, 18, 35, 38, 87, 552, 563
Sau3AI GATC 5 cut(s) 178, 266, 310, 542, 586
Sau96I GGNCC 2 cut(s) 48, 485
SchI GAGTC 1 cut(s) 367
ScrFI CCNGG 1 cut(s) 529
SduI GDGCHC 3 cut(s) 173, 265, 330
SetI ASST 8 cut(s) 107, 178, 186, 327, 390, 461, 514, 533
SfaNI GCATC 3 cut(s) 170, 217, 488
SfcI CTRYAG 1 cut(s) 305
Sfr303I CCGCGG 1 cut(s) 35
SgrBI CCGCGG 1 cut(s) 35
SinI GGWCC 2 cut(s) 48, 485
SmiMI CAYNNNNRTG 3 cut(s) 381, 430, 570
SmlI CTYRAG 3 cut(s) 278, 337, 568
SmoI CTYRAG 3 cut(s) 278, 337, 568
Sse9I AATT 3 cut(s) 212, 220, 474
SsiI CCGC 6 cut(s) 6, 13, 32, 34, 57, 87
SspMI CTAG 2 cut(s) 270, 595
StyD4I CCNGG 1 cut(s) 527
TaiI ACGT 1 cut(s) 327
TaqI TCGA 2 cut(s) 63, 403
TasI AATT 3 cut(s) 212, 220, 474
TauI GCSGC 3 cut(s) 8, 37, 89
TfiI GAWTC 3 cut(s) 72, 440, 452
Tru1I TTAA 2 cut(s) 230, 296
Tru9I TTAA 2 cut(s) 230, 296
TseI GCWGC 4 cut(s) 17, 37, 551, 562
TspDTI ATGAA 4 cut(s) 302, 340, 411, 504
Tth111I GACNNNGTC 1 cut(s) 52
VneI GTGCAC 1 cut(s) 326
VpaK11BI GGWCC 2 cut(s) 48, 485
XmiI GTMKAC 1 cut(s) 274
XmnI GAANNNNTTC 1 cut(s) 451
XspI CTAG 2 cut(s) 270, 595
Zsp2I ATGCAT 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.