Rroxscaffold_4G00301480

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0011591
Physical Location & Seq
Forward (+)
21847537 .. 21848770
1234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301480.1

Sequence Viewer

Length: 849 bp
ATGGCCCCAAATGTTGGAACCCACGTAAAACGACGCCTGAGCCGCCGAGACTGGACCGAACTTCCGGGCGTCGTAACGGTATCGATACTGTCACGTCTTGGAGACATCGAGTCCCTGACGGTGGCTGAGATGGTGTGCAAGACGTGGCGTGAAATCTGCAAAGACCCTATGCTGTGGCGCACCATCGACTTCATGAGCAACCATACAAAGTTCATGTTCGGGCCGCCGTTGCGTTACGATATAGTGAAGATGTGCCGCCGCGCCATATACCGTAGCTGTGGTAGTTTTGTCTACGTCAACGTCGACTACTTTTGCAACAATGAACTGTTAAAGTATATCACTAACAGTTCAGCCGGATTTATTAGACACCTCAGTTTGTTCAATTGTGGCATTATATCTGATGAGGTGTTGTGTGAAGCAGCTTTGAGACTTCCAGTTTTGGAGGAACTTGACATTTCGATGTGCAAATTCTCCAGTATAGCTATACATATAGTTGGGCTCTGCTGCCCTCTTTTGAAATCATTCAAATTTAACACCCCGAATGGAAGAAAGTCTTCCGGGCAGTATTATAGCGACGAGGATGCAGTTGCTATATCAGGAACCATGCATGGTTTACTCCTCCTTGAGATTCGTAGGAATGACCTGACTTGCAATGGCTTGGCTAAAATTCTTGATTGTTGTCCTCAACTCGAGATCCTTGATTTGCGCAAGTGTAAAAACCTAAAATATATGAGCACAGATTTGGAAATAAGATGTGTTGAAAGAATTAAGAAGTTGTGGCTCCCGCGGCTCCCGATCGATCCCTCTAGTATATCGGACTTGAATTTGGTGGGCAAACCATGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

282

Amino Acids

32.15

Weight (kDa)

8.65

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 18 - 64 5.4e-11 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 707
AccB7I CCANNNNNTGG 1 cut(s) 14
AccI GTMKAC 2 cut(s) 291, 303
AccII CGCG 2 cut(s) 261, 787
AciI CCGC 6 cut(s) 43, 224, 256, 259, 785, 787
AclWI GGATC 2 cut(s) 688, 794
AcsI RAATTY 4 cut(s) 467, 527, 666, 823
AcyI GRCGYC 2 cut(s) 34, 69
AfiI CCNNNNNNNGG 2 cut(s) 14, 121
AgsI TTSAA 5 cut(s) 382, 517, 526, 761, 823
AhdI GACNNNNNGTC 1 cut(s) 109
AjiI CACGTC 2 cut(s) 95, 144
AluBI AGCT 3 cut(s) 276, 422, 482
AluI AGCT 3 cut(s) 276, 422, 482
Alw21I GWGCWC 1 cut(s) 737
Alw26I GTCTC 3 cut(s) 42, 96, 421
AlwI GGATC 2 cut(s) 688, 794
Ama87I CYCGRG 1 cut(s) 689
AoxI GGCC 2 cut(s) 3, 221
ApeKI GCWGC 2 cut(s) 419, 504
ApoI RAATTY 4 cut(s) 467, 527, 666, 823
Asp700I GAANNNNTTC 2 cut(s) 521, 553
AspLEI GCGC 3 cut(s) 180, 263, 708
AspS9I GGNCC 3 cut(s) 4, 54, 221
AsuC2I CCSGG 2 cut(s) 66, 559
AvaI CYCGRG 1 cut(s) 689
AvaII GGWCC 1 cut(s) 54
BanII GRGCYC 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 546
Bbv12I GWGCWC 1 cut(s) 737
BbvI GCAGC 2 cut(s) 431, 491
BccI CCATC 2 cut(s) 124, 191
BceAI ACGGC 1 cut(s) 211
BcgI CGANNNNNNTGC 2 cut(s) 563, 597
BcnI CCSGG 2 cut(s) 66, 559
BcoDI GTCTC 3 cut(s) 42, 96, 421
BfaI CTAG 1 cut(s) 807
BisI GCNGC 7 cut(s) 43, 224, 256, 259, 420, 505, 788
BlsI GCNGC 7 cut(s) 44, 225, 257, 260, 421, 506, 789
Bme1390I CCNGG 2 cut(s) 66, 559
Bme18I GGWCC 1 cut(s) 54
BmeRI GACNNNNNGTC 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 689
BmgBI CACGTC 2 cut(s) 95, 144
BmgT120I GGNCC 3 cut(s) 4, 54, 221
BmiI GGNNCC 5 cut(s) 6, 19, 601, 782, 791
BmrFI CCNGG 2 cut(s) 66, 559
BmsI GCATC 1 cut(s) 571
BpiI GAAGAC 1 cut(s) 546
BpmI CTGGAG 1 cut(s) 457
Bpu10I CCTNAGC 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 644
BpuMI CCSGG 2 cut(s) 66, 559
Bsa29I ATCGAT 2 cut(s) 83, 798
BsaAI YACGTR 1 cut(s) 25
BsaHI GRCGYC 2 cut(s) 34, 69
BsaJI CCNNGG 1 cut(s) 785
Bsc4I CCNNNNNNNGG 2 cut(s) 14, 121
Bse1I ACTGG 3 cut(s) 56, 434, 474
Bse3DI GCAATG 1 cut(s) 658
BseCI ATCGAT 2 cut(s) 83, 798
BseDI CCNNGG 1 cut(s) 785
BseGI GGATG 1 cut(s) 586
BseLI CCNNNNNNNGG 2 cut(s) 14, 121
BseMI GCAATG 1 cut(s) 658
BseMII CTCAG 3 cut(s) 29, 117, 385
BseNI ACTGG 3 cut(s) 56, 434, 474
BseRI GAGGAG 1 cut(s) 608
BseXI GCAGC 2 cut(s) 431, 491
Bsh1236I CGCG 2 cut(s) 261, 787
Bsh1285I CGRYCG 1 cut(s) 798
BshFI GGCC 2 cut(s) 5, 223
BshVI ATCGAT 2 cut(s) 83, 798
BsiEI CGRYCG 1 cut(s) 798
BsiHKAI GWGCWC 1 cut(s) 737
BsiHKCI CYCGRG 1 cut(s) 689
BsiSI CCGG 3 cut(s) 65, 354, 558
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 2 cut(s) 14, 121
BsmAI GTCTC 3 cut(s) 42, 96, 421
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 2 cut(s) 5, 223
BsoBI CYCGRG 1 cut(s) 689
Bsp1286I GDGCHC 2 cut(s) 501, 737
Bsp143I GATC 3 cut(s) 693, 795, 799
BspACI CCGC 6 cut(s) 43, 224, 256, 259, 785, 787
BspANI GGCC 2 cut(s) 5, 223
BspCNI CTCAG 3 cut(s) 30, 118, 384
BspDI ATCGAT 2 cut(s) 83, 798
BspFNI CGCG 2 cut(s) 261, 787
BspHI TCATGA 1 cut(s) 192
BspLI GGNNCC 5 cut(s) 6, 19, 601, 782, 791
BspPI GGATC 2 cut(s) 688, 794
BsrDI GCAATG 1 cut(s) 658
BsrI ACTGG 3 cut(s) 56, 434, 474
BssECI CCNNGG 1 cut(s) 785
BssMI GATC 3 cut(s) 693, 795, 799
BssNI GRCGYC 2 cut(s) 34, 69
Bst4CI ACNGT 6 cut(s) 79, 90, 121, 272, 327, 347
BstACI GRCGYC 2 cut(s) 34, 69
BstBAI YACGTR 1 cut(s) 25
BstDEI CTNAG 3 cut(s) 38, 126, 371
BstDSI CCRYGG 1 cut(s) 785
BstF5I GGATG 1 cut(s) 586
BstFNI CGCG 2 cut(s) 261, 787
BstHHI GCGC 3 cut(s) 180, 263, 708
BstKTI GATC 3 cut(s) 696, 798, 802
BstMAI GTCTC 3 cut(s) 42, 96, 421
BstMBI GATC 3 cut(s) 693, 795, 799
BstMCI CGRYCG 1 cut(s) 798
BstMWI GCNNNNNNNGC 3 cut(s) 42, 229, 787
BstSCI CCNGG 2 cut(s) 64, 557
BstUI CGCG 2 cut(s) 261, 787
BstV1I GCAGC 2 cut(s) 431, 491
BstV2I GAAGAC 1 cut(s) 546
BstX2I RGATCY 1 cut(s) 693
BstYI RGATCY 1 cut(s) 693
Bsu15I ATCGAT 2 cut(s) 83, 798
BsuRI GGCC 2 cut(s) 5, 223
BsuTUI ATCGAT 2 cut(s) 83, 798
BtgI CCRYGG 1 cut(s) 785
BtrI CACGTC 2 cut(s) 95, 144
BtsCI GGATG 1 cut(s) 586
CciI TCATGA 1 cut(s) 192
CfoI GCGC 3 cut(s) 180, 263, 708
Cfr13I GGNCC 3 cut(s) 4, 54, 221
Cfr42I CCGCGG 1 cut(s) 788
ClaI ATCGAT 2 cut(s) 83, 798
CseI GACGC 2 cut(s) 42, 58
CviAII CATG 5 cut(s) 193, 214, 604, 608, 840
DdeI CTNAG 3 cut(s) 38, 126, 371
DpnI GATC 3 cut(s) 695, 797, 801
DpnII GATC 3 cut(s) 693, 795, 799
DriI GACNNNNNGTC 1 cut(s) 109
Eam1105I GACNNNNNGTC 1 cut(s) 109
Eco24I GRGCYC 1 cut(s) 501
Eco47I GGWCC 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 689
EcoT22I ATGCAT 1 cut(s) 609
EcoT38I GRGCYC 1 cut(s) 501
FaeI CATG 5 cut(s) 196, 217, 607, 611, 843
FalI AAGNNNNNCTT 2 cut(s) 538, 570
FaqI GGGAC 1 cut(s) 97
FatI CATG 5 cut(s) 192, 213, 603, 607, 839
FauI CCCGC 1 cut(s) 792
FblI GTMKAC 2 cut(s) 291, 303
Fnu4HI GCNGC 7 cut(s) 43, 224, 256, 259, 420, 505, 788
FokI GGATG 1 cut(s) 593
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 7 cut(s) 43, 224, 256, 259, 420, 505, 788
FspBI CTAG 1 cut(s) 807
FspI TGCGCA 1 cut(s) 707
GlaI GCGC 3 cut(s) 179, 262, 707
GluI GCNGC 7 cut(s) 43, 224, 256, 259, 420, 505, 788
GsuI CTGGAG 1 cut(s) 457
HaeIII GGCC 2 cut(s) 5, 223
HapII CCGG 3 cut(s) 65, 354, 558
HgaI GACGC 2 cut(s) 42, 58
HhaI GCGC 3 cut(s) 180, 263, 708
Hin1I GRCGYC 2 cut(s) 34, 69
Hin1II CATG 5 cut(s) 196, 217, 607, 611, 843
Hin6I GCGC 3 cut(s) 178, 261, 706
HinP1I GCGC 3 cut(s) 178, 261, 706
HincII GTYRAC 2 cut(s) 298, 304
HindII GTYRAC 2 cut(s) 298, 304
HinfI GANTC 2 cut(s) 110, 628
HpaII CCGG 3 cut(s) 65, 354, 558
Hpy166II GTNNAC 4 cut(s) 292, 298, 304, 614
Hpy188I TCNGA 2 cut(s) 400, 817
Hpy188III TCNNGA 5 cut(s) 193, 597, 671, 691, 793
Hpy8I GTNNAC 4 cut(s) 292, 298, 304, 614
Hpy99I CGWCG 4 cut(s) 36, 74, 305, 578
HpyCH4III ACNGT 6 cut(s) 79, 90, 121, 272, 327, 347
HpyCH4IV ACGT 5 cut(s) 24, 94, 143, 294, 300
HpyCH4V TGCA 7 cut(s) 138, 159, 315, 465, 584, 607, 651
HpyF10VI GCNNNNNNNGC 3 cut(s) 42, 229, 787
HpyF3I CTNAG 3 cut(s) 38, 126, 371
HpySE526I ACGT 5 cut(s) 24, 94, 143, 294, 300
Hsp92I GRCGYC 2 cut(s) 34, 69
Hsp92II CATG 5 cut(s) 196, 217, 607, 611, 843
HspAI GCGC 3 cut(s) 178, 261, 706
KspI CCGCGG 1 cut(s) 788
Kzo9I GATC 3 cut(s) 693, 795, 799
LmnI GCTCC 2 cut(s) 786, 795
Lsp1109I GCAGC 2 cut(s) 431, 491
LweI GCATC 1 cut(s) 571
MaeI CTAG 1 cut(s) 807
MaeII ACGT 5 cut(s) 24, 94, 143, 294, 300
MaeIII GTNAC 3 cut(s) 73, 90, 233
MalI GATC 3 cut(s) 695, 797, 801
MboI GATC 3 cut(s) 693, 795, 799
MboII GAAGA 3 cut(s) 259, 546, 558
MfeI CAATTG 1 cut(s) 382
MflI RGATCY 1 cut(s) 693
MhlI GDGCHC 2 cut(s) 501, 737
MluCI AATT 6 cut(s) 382, 467, 527, 666, 765, 823
MlyI GAGTC 1 cut(s) 119
MnlI CCTC 8 cut(s) 380, 397, 436, 519, 571, 629, 693, 814
Mph1103I ATGCAT 1 cut(s) 609
MroXI GAANNNNTTC 2 cut(s) 521, 553
MseI TTAA 4 cut(s) 329, 531, 768, 847
MslI CAYNNNNRTG 1 cut(s) 458
MspA1I CMGCKG 1 cut(s) 787
MspI CCGG 3 cut(s) 65, 354, 558
MspR9I CCNGG 2 cut(s) 66, 559
MunI CAATTG 1 cut(s) 382
MvnI CGCG 2 cut(s) 261, 787
MwoI GCNNNNNNNGC 3 cut(s) 42, 229, 787
NciI CCSGG 2 cut(s) 66, 559
NdeII GATC 3 cut(s) 693, 795, 799
NlaIII CATG 5 cut(s) 196, 217, 607, 611, 843
NlaIV GGNNCC 5 cut(s) 6, 19, 601, 782, 791
NmeAIII GCCGAG 1 cut(s) 71
NmuCI GTSAC 1 cut(s) 90
NsbI TGCGCA 1 cut(s) 707
NsiI ATGCAT 1 cut(s) 609
PaeR7I CTCGAG 1 cut(s) 689
PagI TCATGA 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PdmI GAANNNNTTC 2 cut(s) 521, 553
PfeI GAWTC 1 cut(s) 628
PflMI CCANNNNNTGG 1 cut(s) 14
PkrI GCNGC 7 cut(s) 44, 225, 257, 260, 421, 506, 789
Ple19I CGATCG 1 cut(s) 798
PleI GAGTC 1 cut(s) 118
PpsI GAGTC 1 cut(s) 118
Ppu21I YACGTR 1 cut(s) 25
PspN4I GGNNCC 5 cut(s) 6, 19, 601, 782, 791
PspPI GGNCC 3 cut(s) 4, 54, 221
PsuI RGATCY 1 cut(s) 693
PvuI CGATCG 1 cut(s) 798
RseI CAYNNNNRTG 1 cut(s) 458
SacII CCGCGG 1 cut(s) 788
SalI GTCGAC 1 cut(s) 302
SaqAI TTAA 4 cut(s) 329, 531, 768, 847
SatI GCNGC 7 cut(s) 43, 224, 256, 259, 420, 505, 788
Sau3AI GATC 3 cut(s) 693, 795, 799
Sau96I GGNCC 3 cut(s) 4, 54, 221
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 2 cut(s) 66, 559
SduI GDGCHC 2 cut(s) 501, 737
SfaNI GCATC 1 cut(s) 571
Sfr274I CTCGAG 1 cut(s) 689
Sfr303I CCGCGG 1 cut(s) 788
SgrBI CCGCGG 1 cut(s) 788
SinI GGWCC 1 cut(s) 54
SlaI CTCGAG 1 cut(s) 689
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 2 cut(s) 623, 689
SmoI CTYRAG 2 cut(s) 623, 689
Sse9I AATT 6 cut(s) 382, 467, 527, 666, 765, 823
SsiI CCGC 6 cut(s) 43, 224, 256, 259, 785, 787
SspMI CTAG 1 cut(s) 807
StyD4I CCNGG 2 cut(s) 64, 557
TaaI ACNGT 6 cut(s) 79, 90, 121, 272, 327, 347
TaiI ACGT 5 cut(s) 27, 97, 146, 297, 303
TaqI TCGA 7 cut(s) 83, 108, 186, 303, 458, 690, 798
TaqII GACCGA 1 cut(s) 71
TasI AATT 6 cut(s) 382, 467, 527, 666, 765, 823
TauI GCSGC 5 cut(s) 45, 226, 258, 261, 790
TfiI GAWTC 1 cut(s) 628
Tru1I TTAA 4 cut(s) 329, 531, 768, 847
Tru9I TTAA 4 cut(s) 329, 531, 768, 847
TseFI GTSAC 1 cut(s) 90
TseI GCWGC 2 cut(s) 419, 504
Tsp45I GTSAC 1 cut(s) 90
TspDTI ATGAA 3 cut(s) 181, 202, 336
Van91I CCANNNNNTGG 1 cut(s) 14
VpaK11BI GGWCC 1 cut(s) 54
XapI RAATTY 4 cut(s) 467, 527, 666, 823
XhoI CTCGAG 1 cut(s) 689
XmiI GTMKAC 2 cut(s) 291, 303
XmnI GAANNNNTTC 2 cut(s) 521, 553
XspI CTAG 1 cut(s) 807
Zsp2I ATGCAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.