Rroxscaffold_6G00401320

Alpha-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0003938
Physical Location & Seq
Forward (+)
23654098 .. 23655304
1207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00401320.1

Sequence Viewer

Length: 345 bp
ATGGGACGGAACCCTTCAAGGAGAAACTCGACTAAGGAGAGATTTGCTCAAGGAAAGATCCGAGTGAGCTCGGCGGATTATGTCCACAAGAAGGGTCTTAAGCTAGGAATCTACTCGGATGCGAGTCGTACTTGCAGCAAAACTATGCCTGGTTCACTTGGTCATGAAGAACAAGATGCCAATACCTTTGCTGCTTGGGGCATCGATTATTTGAAATATGATAACTGTTATAATGATGGCTCAAACCCCGTAATGACCCGAGCTCTAATGAAAGCAGCCGTCCCATATTCTTCTCTCTCTGCGAATGGGGAGATATGCACCTCGCTTTATGGGGTGCCAAGTTAG

Protein Analysis

114

Amino Acids

12.46

Weight (kDa)

9.22

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Melibiase_2 PF16499 8 - 87 6.9e-21 Alpha galactosidase A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 231
AccB1I GGYRCC 1 cut(s) 334
AciI CCGC 1 cut(s) 74
AclWI GGATC 1 cut(s) 52
AfaI GTAC 1 cut(s) 130
AfiI CCNNNNNNNGG 1 cut(s) 91
AflII CTTAAG 1 cut(s) 98
AgsI TTSAA 2 cut(s) 18, 214
AjnI CCWGG 1 cut(s) 148
AluBI AGCT 3 cut(s) 69, 103, 263
AluI AGCT 3 cut(s) 69, 103, 263
Alw21I GWGCWC 2 cut(s) 71, 265
AlwI GGATC 1 cut(s) 52
Ama87I CYCGRG 1 cut(s) 258
ApeKI GCWGC 3 cut(s) 135, 191, 275
AvaI CYCGRG 1 cut(s) 258
BanI GGYRCC 1 cut(s) 334
BanII GRGCYC 2 cut(s) 71, 265
Bbv12I GWGCWC 2 cut(s) 71, 265
BbvI GCAGC 3 cut(s) 147, 178, 287
BccI CCATC 1 cut(s) 230
BceAI ACGGC 1 cut(s) 263
BciT130I CCWGG 1 cut(s) 150
BfaI CTAG 1 cut(s) 104
BfrI CTTAAG 1 cut(s) 98
BisI GCNGC 3 cut(s) 136, 192, 276
BlsI GCNGC 3 cut(s) 137, 193, 277
Bme1390I CCNGG 1 cut(s) 150
BmeT110I CYCGRG 1 cut(s) 258
BmiI GGNNCC 2 cut(s) 11, 336
BmrFI CCNGG 1 cut(s) 150
BmsI GCATC 3 cut(s) 109, 166, 210
BplI GAGNNNNNCTC 2 cut(s) 31, 63
BpuEI CTTGAG 1 cut(s) 33
Bsa29I ATCGAT 1 cut(s) 204
Bsc4I CCNNNNNNNGG 1 cut(s) 91
BseBI CCWGG 1 cut(s) 150
BseCI ATCGAT 1 cut(s) 204
BseGI GGATG 1 cut(s) 124
BseLI CCNNNNNNNGG 1 cut(s) 91
BseXI GCAGC 3 cut(s) 147, 178, 287
BshNI GGYRCC 1 cut(s) 334
BshVI ATCGAT 1 cut(s) 204
BsiHKAI GWGCWC 2 cut(s) 71, 265
BsiHKCI CYCGRG 1 cut(s) 258
BslFI GGGAC 2 cut(s) 18, 266
BslI CCNNNNNNNGG 1 cut(s) 91
BsmFI GGGAC 2 cut(s) 18, 266
BsoBI CYCGRG 1 cut(s) 258
Bsp1286I GDGCHC 2 cut(s) 71, 265
Bsp143I GATC 1 cut(s) 57
BspACI CCGC 1 cut(s) 74
BspDI ATCGAT 1 cut(s) 204
BspHI TCATGA 1 cut(s) 163
BspLI GGNNCC 2 cut(s) 11, 336
BspPI GGATC 1 cut(s) 52
BspT107I GGYRCC 1 cut(s) 334
BspTI CTTAAG 1 cut(s) 98
BssMI GATC 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 150
Bst4CI ACNGT 1 cut(s) 227
BstAFI CTTAAG 1 cut(s) 98
BstDEI CTNAG 1 cut(s) 33
BstF5I GGATG 1 cut(s) 124
BstKTI GATC 1 cut(s) 60
BstMBI GATC 1 cut(s) 57
BstNI CCWGG 1 cut(s) 150
BstSCI CCNGG 1 cut(s) 148
BstV1I GCAGC 3 cut(s) 147, 178, 287
BstX2I RGATCY 1 cut(s) 57
BstYI RGATCY 1 cut(s) 57
Bsu15I ATCGAT 1 cut(s) 204
BsuTUI ATCGAT 1 cut(s) 204
BtsCI GGATG 1 cut(s) 124
CciI TCATGA 1 cut(s) 163
ClaI ATCGAT 1 cut(s) 204
Csp6I GTAC 1 cut(s) 129
CviAII CATG 1 cut(s) 164
CviJI RGCY 5 cut(s) 69, 103, 240, 263, 278
CviKI_1 RGCY 5 cut(s) 69, 103, 240, 263, 278
CviQI GTAC 1 cut(s) 129
DdeI CTNAG 1 cut(s) 33
DpnI GATC 1 cut(s) 59
DpnII GATC 1 cut(s) 57
EciI GGCGGA 1 cut(s) 89
Ecl136II GAGCTC 2 cut(s) 69, 263
Eco24I GRGCYC 2 cut(s) 71, 265
Eco53kI GAGCTC 2 cut(s) 69, 263
Eco88I CYCGRG 1 cut(s) 258
EcoICRI GAGCTC 2 cut(s) 69, 263
EcoRII CCWGG 1 cut(s) 148
EcoT38I GRGCYC 2 cut(s) 71, 265
FaeI CATG 1 cut(s) 167
FaiI YATR 8 cut(s) 81, 146, 165, 219, 231, 286, 316, 330
FaqI GGGAC 2 cut(s) 18, 266
FatI CATG 1 cut(s) 163
Fnu4HI GCNGC 3 cut(s) 136, 192, 276
FokI GGATG 1 cut(s) 131
FriOI GRGCYC 2 cut(s) 71, 265
Fsp4HI GCNGC 3 cut(s) 136, 192, 276
FspBI CTAG 1 cut(s) 104
GluI GCNGC 3 cut(s) 136, 192, 276
Hin1II CATG 1 cut(s) 167
HinfI GANTC 2 cut(s) 108, 124
Hpy166II GTNNAC 2 cut(s) 85, 155
Hpy188I TCNGA 2 cut(s) 62, 118
Hpy188III TCNNGA 1 cut(s) 164
Hpy8I GTNNAC 2 cut(s) 85, 155
HpyAV CCTTC 2 cut(s) 24, 85
HpyCH4III ACNGT 1 cut(s) 227
HpyCH4V TGCA 2 cut(s) 135, 318
HpyF3I CTNAG 1 cut(s) 33
Hsp92II CATG 1 cut(s) 167
Kzo9I GATC 1 cut(s) 57
LpnPI CCDG 2 cut(s) 135, 162
Lsp1109I GCAGC 3 cut(s) 147, 178, 287
LweI GCATC 3 cut(s) 109, 166, 210
MaeI CTAG 1 cut(s) 104
MalI GATC 1 cut(s) 59
MboI GATC 1 cut(s) 57
MboII GAAGA 2 cut(s) 179, 282
MflI RGATCY 1 cut(s) 57
MhlI GDGCHC 2 cut(s) 71, 265
MlyI GAGTC 1 cut(s) 133
MnlI CCTC 1 cut(s) 331
MseI TTAA 1 cut(s) 99
MspCI CTTAAG 1 cut(s) 98
MspR9I CCNGG 1 cut(s) 150
MvaI CCWGG 1 cut(s) 150
NdeII GATC 1 cut(s) 57
NlaIII CATG 1 cut(s) 167
NlaIV GGNNCC 2 cut(s) 11, 336
NmeAIII GCCGAG 1 cut(s) 50
PagI TCATGA 1 cut(s) 163
PfeI GAWTC 1 cut(s) 108
PkrI GCNGC 3 cut(s) 137, 193, 277
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
PsiI TTATAA 1 cut(s) 231
Psp124BI GAGCTC 2 cut(s) 71, 265
Psp6I CCWGG 1 cut(s) 148
PspGI CCWGG 1 cut(s) 148
PspN4I GGNNCC 2 cut(s) 11, 336
PsuI RGATCY 1 cut(s) 57
RsaI GTAC 1 cut(s) 130
RsaNI GTAC 1 cut(s) 129
SacI GAGCTC 2 cut(s) 71, 265
SaqAI TTAA 1 cut(s) 99
SatI GCNGC 3 cut(s) 136, 192, 276
Sau3AI GATC 1 cut(s) 57
SchI GAGTC 1 cut(s) 133
ScrFI CCNGG 1 cut(s) 150
SduI GDGCHC 2 cut(s) 71, 265
SetI ASST 5 cut(s) 71, 105, 188, 265, 323
SfaNI GCATC 3 cut(s) 109, 166, 210
SmlI CTYRAG 2 cut(s) 48, 98
SmoI CTYRAG 2 cut(s) 48, 98
SsiI CCGC 1 cut(s) 74
SspMI CTAG 1 cut(s) 104
SstI GAGCTC 2 cut(s) 71, 265
StyD4I CCNGG 1 cut(s) 148
TaaI ACNGT 1 cut(s) 227
TaqI TCGA 2 cut(s) 29, 204
TfiI GAWTC 1 cut(s) 108
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TseI GCWGC 3 cut(s) 135, 191, 275
TspDTI ATGAA 2 cut(s) 180, 284
TspGWI ACGGA 1 cut(s) 22
Vha464I CTTAAG 1 cut(s) 98
XspI CTAG 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.