Rroxscaffold_7G00161230

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008911
Physical Location & Seq
Forward (+)
4024151 .. 4033885
9735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00161230.1

Sequence Viewer

Length: 552 bp
ATGGAAAATTGTTTGCTGAGTGACGATGAAGTAGGCCTAATCTTCGAAAGGTTAAGCGACCCAGATGATAGAAAATCCTTCTCCAAAGTCTGCAAGCAATGGCAGAGAGTGGAGGGTCTAACCCGACCATCAATTCTTCTCCTCGGACCTGAATTTCTCAGTCAATTCCCCAATTTAGTCACATTCGAAACGTCCAAGGATATCAGCGAAACCGACCTCAAATTCATAGCTGAAACCTGTCCCAATATCGAGTCAATTTCACCGTATTTTCGAACCCGATCGGATTGGTCAACCATAGTCACTTGTATGTTTTCTTGGTTGATTGATGGCGTGATGACCGCAAAACTTGCTCATTTTTTTACATCACATGCATTGCTTGGAGGACCTGAATTTGGCTCACTTGTCATCAGATGTCACCGACATTGGAGATGTGGCAATCTCGGGTTGTCAAACACTCAAGAGATTGAACTTGTACGCGCGGGTCGAGAGTGTCAGACCGCACCATTGTTGCTCTTGCTGAAAATTGCCTCAGCTTGGAGTGGCTTGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6

Protein Analysis

183

Amino Acids

20.67

Weight (kDa)

5.58

Isoelectric Point (pI)

38.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box_5 PF18511 13 - 42 6.7e-07 F-box
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 477, 479
AciI CCGC 3 cut(s) 339, 479, 498
AcsI RAATTY 3 cut(s) 152, 221, 389
AfaI GTAC 1 cut(s) 474
AfiI CCNNNNNNNGG 2 cut(s) 392, 534
AgsI TTSAA 1 cut(s) 467
AluBI AGCT 2 cut(s) 230, 533
AluI AGCT 2 cut(s) 230, 533
Ama87I CYCGRG 1 cut(s) 440
AoxI GGCC 1 cut(s) 34
ApoI RAATTY 3 cut(s) 152, 221, 389
AspLEI GCGC 1 cut(s) 479
AspS9I GGNCC 2 cut(s) 146, 383
AsuHPI GGTGA 2 cut(s) 252, 407
AsuII TTCGAA 3 cut(s) 45, 186, 271
AvaI CYCGRG 1 cut(s) 440
AvaII GGWCC 2 cut(s) 146, 383
BbvCI CCTCAGC 1 cut(s) 529
BccI CCATC 2 cut(s) 136, 320
Bme18I GGWCC 2 cut(s) 146, 383
BmeT110I CYCGRG 1 cut(s) 440
BmgT120I GGNCC 2 cut(s) 146, 383
Bpu10I CCTNAGC 1 cut(s) 529
Bpu14I TTCGAA 3 cut(s) 45, 186, 271
BpuEI CTTGAG 1 cut(s) 441
BsaJI CCNNGG 2 cut(s) 142, 195
Bsc4I CCNNNNNNNGG 2 cut(s) 392, 534
Bse3DI GCAATG 2 cut(s) 104, 371
BseDI CCNNGG 2 cut(s) 142, 195
BseLI CCNNNNNNNGG 2 cut(s) 392, 534
BseMI GCAATG 2 cut(s) 104, 371
BseMII CTCAG 3 cut(s) 8, 172, 543
BseRI GAGGAG 1 cut(s) 131
Bsh1236I CGCG 2 cut(s) 477, 479
Bsh1285I CGRYCG 1 cut(s) 281
BshFI GGCC 1 cut(s) 36
BsiEI CGRYCG 1 cut(s) 281
BsiHKCI CYCGRG 1 cut(s) 440
BslFI GGGAC 1 cut(s) 225
BslI CCNNNNNNNGG 2 cut(s) 392, 534
BsmFI GGGAC 1 cut(s) 225
BsnI GGCC 1 cut(s) 36
BsoBI CYCGRG 1 cut(s) 440
Bsp119I TTCGAA 3 cut(s) 45, 186, 271
Bsp143I GATC 1 cut(s) 278
BspACI CCGC 3 cut(s) 339, 479, 498
BspANI GGCC 1 cut(s) 36
BspCNI CTCAG 3 cut(s) 9, 171, 542
BspFNI CGCG 2 cut(s) 477, 479
BspT104I TTCGAA 3 cut(s) 45, 186, 271
BsrDI GCAATG 2 cut(s) 104, 371
BssECI CCNNGG 2 cut(s) 142, 195
BssMI GATC 1 cut(s) 278
BssT1I CCWWGG 1 cut(s) 195
Bst4CI ACNGT 1 cut(s) 264
BstAPI GCANNNNNTGC 1 cut(s) 347
BstBI TTCGAA 3 cut(s) 45, 186, 271
BstC8I GCNNGC 1 cut(s) 95
BstDEI CTNAG 3 cut(s) 17, 158, 529
BstFNI CGCG 2 cut(s) 477, 479
BstHHI GCGC 1 cut(s) 479
BstKTI GATC 1 cut(s) 281
BstMBI GATC 1 cut(s) 278
BstMCI CGRYCG 1 cut(s) 281
BstMWI GCNNNNNNNGC 1 cut(s) 347
BstNSI RCATGY 1 cut(s) 371
BstUI CGCG 2 cut(s) 477, 479
BsuRI GGCC 1 cut(s) 36
Cac8I GCNNGC 1 cut(s) 95
CfoI GCGC 1 cut(s) 479
Cfr13I GGNCC 2 cut(s) 146, 383
Csp6I GTAC 1 cut(s) 473
CviAII CATG 1 cut(s) 368
CviJI RGCY 5 cut(s) 36, 230, 396, 533, 543
CviKI_1 RGCY 5 cut(s) 36, 230, 396, 533, 543
CviQI GTAC 1 cut(s) 473
DdeI CTNAG 3 cut(s) 17, 158, 529
DpnI GATC 1 cut(s) 280
DpnII GATC 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 195
Eco147I AGGCCT 1 cut(s) 36
Eco32I GATATC 1 cut(s) 202
Eco47I GGWCC 2 cut(s) 146, 383
Eco88I CYCGRG 1 cut(s) 440
EcoO109I RGGNCCY 1 cut(s) 383
EcoRV GATATC 1 cut(s) 202
EcoT14I CCWWGG 1 cut(s) 195
EcoT22I ATGCAT 1 cut(s) 373
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 1 cut(s) 371
FaiI YATR 4 cut(s) 227, 296, 308, 369
FaqI GGGAC 1 cut(s) 225
FatI CATG 1 cut(s) 367
FauI CCCGC 1 cut(s) 472
GlaI GCGC 1 cut(s) 478
HaeIII GGCC 1 cut(s) 36
HhaI GCGC 1 cut(s) 479
Hin1II CATG 1 cut(s) 371
Hin6I GCGC 1 cut(s) 477
HinP1I GCGC 1 cut(s) 477
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HinfI GANTC 1 cut(s) 251
HphI GGTGA 2 cut(s) 252, 407
Hpy166II GTNNAC 1 cut(s) 291
Hpy188I TCNGA 4 cut(s) 146, 283, 410, 495
Hpy188III TCNNGA 2 cut(s) 458, 485
Hpy8I GTNNAC 1 cut(s) 291
HpyAV CCTTC 1 cut(s) 88
HpyCH4III ACNGT 1 cut(s) 264
HpyCH4IV ACGT 1 cut(s) 191
HpyCH4V TGCA 2 cut(s) 93, 371
HpyF10VI GCNNNNNNNGC 1 cut(s) 347
HpyF3I CTNAG 3 cut(s) 17, 158, 529
HpySE526I ACGT 1 cut(s) 191
Hsp92II CATG 1 cut(s) 371
HspAI GCGC 1 cut(s) 477
Kzo9I GATC 1 cut(s) 278
LpnPI CCDG 4 cut(s) 75, 162, 250, 399
MaeII ACGT 1 cut(s) 191
MaeIII GTNAC 4 cut(s) 20, 178, 298, 413
MalI GATC 1 cut(s) 280
MboI GATC 1 cut(s) 278
MboII GAAGA 2 cut(s) 34, 128
MluCI AATT 9 cut(s) 7, 132, 152, 164, 172, 221, 255, 389, 522
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 5 cut(s) 106, 152, 227, 374, 538
Mph1103I ATGCAT 1 cut(s) 373
MseI TTAA 2 cut(s) 53, 550
MslI CAYNNNNRTG 1 cut(s) 305
MvnI CGCG 2 cut(s) 477, 479
MwoI GCNNNNNNNGC 1 cut(s) 347
NdeII GATC 1 cut(s) 278
NlaIII CATG 1 cut(s) 371
NmuCI GTSAC 4 cut(s) 20, 178, 298, 413
NsiI ATGCAT 1 cut(s) 373
NspI RCATGY 1 cut(s) 371
NspV TTCGAA 3 cut(s) 45, 186, 271
PceI AGGCCT 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 481
Ple19I CGATCG 1 cut(s) 281
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PpuMI RGGWCCY 1 cut(s) 383
Psp5II RGGWCCY 1 cut(s) 383
PspPI GGNCC 2 cut(s) 146, 383
PspPPI RGGWCCY 1 cut(s) 383
PvuI CGATCG 1 cut(s) 281
RsaI GTAC 1 cut(s) 474
RsaNI GTAC 1 cut(s) 473
RseI CAYNNNNRTG 1 cut(s) 305
SaqAI TTAA 2 cut(s) 53, 550
Sau3AI GATC 1 cut(s) 278
Sau96I GGNCC 2 cut(s) 146, 383
SchI GAGTC 1 cut(s) 260
SetI ASST 8 cut(s) 53, 151, 194, 219, 232, 239, 388, 535
SfuI TTCGAA 3 cut(s) 45, 186, 271
SinI GGWCC 2 cut(s) 146, 383
SmiMI CAYNNNNRTG 1 cut(s) 305
SmlI CTYRAG 1 cut(s) 456
SmoI CTYRAG 1 cut(s) 456
Sse9I AATT 9 cut(s) 7, 132, 152, 164, 172, 221, 255, 389, 522
SseBI AGGCCT 1 cut(s) 36
SsiI CCGC 3 cut(s) 339, 479, 498
StuI AGGCCT 1 cut(s) 36
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 1 cut(s) 264
TaiI ACGT 1 cut(s) 194
TaqI TCGA 5 cut(s) 45, 186, 249, 271, 484
TasI AATT 9 cut(s) 7, 132, 152, 164, 172, 221, 255, 389, 522
Tru1I TTAA 2 cut(s) 53, 550
Tru9I TTAA 2 cut(s) 53, 550
TseFI GTSAC 4 cut(s) 20, 178, 298, 413
Tsp45I GTSAC 4 cut(s) 20, 178, 298, 413
TspDTI ATGAA 2 cut(s) 42, 214
VpaK11BI GGWCC 2 cut(s) 146, 383
XapI RAATTY 3 cut(s) 152, 221, 389
XceI RCATGY 1 cut(s) 371
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.