Rorug01G0356000

AAA domain (Cdc48 subfamily)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006159
Physical Location & Seq
Forward (+)
47224317 .. 47225647
1331 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0356000.1

Sequence Viewer

Length: 1263 bp
ATGACCTCCAAAGCAGTCAAGGCCACCTTCTACATTTTATTCCTAGCCATCATTACACTTGGATATGCCAACTCAGCTAGGATCCTTGATGAAGCAACTAATCCCCTCCCGACAACTGTCCAGGCTTCAAACCCGATAAATCCCACCACTCCGTTGCCAAGTGGCCAAATCCCTGCCACCACAACCGTGGATGACAACAAGGACGACGCTGAGGCACCAATTCCAGACACTGATGCCCCTGTTGATGATGTCACACCACCCGTTGATGTGGCATCACCAATAGTTACTCCTGTCACCCCCGCGGTGCCAGAAGCTGATGTCACACCACCCACTGATGTGGCATCACCAATAGTCGCTCCTGTTACCCCTGAGGTGCCAGAAGCCGACTCACCCCAACCCGAAGAACCCGACACTACTATCGTGCCAACACCCGTAGCGGGCCCCGTCATCCCCTCCGCAACCGCGCCAATCCCGGGCCCAGCCATCCAGACACCAAAAGTGCCGCTCCCAGTTGCAACACCCGTGGCGGGGCCCGCTACCACCGAAGCGAAACCGCATTTGGCCTTCTTCATGCACGACATTCTTGGAGGATCCCAACCGTCCGTTCGGGTGGTGACCGGGCTCATCGCCAGCACGGTGGCCAACGTCGCCTTCTCGAAACCCAACAACAACATCTTCCCCGTCAGCGGTGGGACCCCCCTAAACAACAACAACCTCAACGGCTTCCTCGACAACAACCGCAACAACCTCCACTCCATCGCCGGCCTCACGGGCACCACCGGCCTCACCAACTCCCAATCCAGCACTGTCATCCAAAACTCCGGCAACAACAACATAGTCAACGGCGGCGACAACCAGCCCTTCGTCACAGCCGGCCAGCTCCCCAACGGCGCCACTCTCCAGAAGCTCATGTTCGGCTCCGTCACCGTCATCGACGACCAACTGACCGAAGGACAGGAGCTCGGCTCCGCCGTGCTAGGAAAAGCTCAGGGTTTCTACTTGGCCAGCTCTTTGGACGGAAACAGCCACACAATGGCGTTCACTGTTCTCATGCACGGCGAGCATGACGTGGAGGACACCATCAGCTTCTTCGGGGTACACCGCACTGCGGCGCCGGTGTCGCATATTGCGGTGATCGGCGGGACCGGGAAGTATGAGAATGCGAATGGTTATGGGGCCATTGAGAGCCTTCATCAGGAGGACCAGCACACAACGGACGGTGTGGATACCATAATGCAGATCAGTGTTTACCTCTCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0010033 GO:0014070 GO:0033993 GO:0042221 GO:0050896 GO:1901700 GO:1902347
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_2 Clp_N

Protein Analysis

420

Amino Acids

43.37

Weight (kDa)

4.34

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 278 - 417 2.8e-27 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 6 cut(s) 214, 304, 373, 773, 890, 1111
AccB7I CCANNNNNTGG 1 cut(s) 1033
AccBSI CCGCTC 1 cut(s) 505
AccII CGCG 2 cut(s) 302, 464
AclWI GGATC 4 cut(s) 76, 89, 585, 598
AcoI YGGCCR 4 cut(s) 163, 639, 874, 1002
AcyI GRCGYC 2 cut(s) 891, 1112
AfaI GTAC 1 cut(s) 1098
AfiI CCNNNNNNNGG 8 cut(s) 437, 473, 527, 528, 686, 1033, 1108, 1195
AgsI TTSAA 1 cut(s) 129
AjiI CACGTC 1 cut(s) 1069
AjnI CCWGG 1 cut(s) 120
AjuI GAANNNNNNNTTGG 6 cut(s) 542, 574, 588, 620, 635, 667
AleI CACNNNNGTG 2 cut(s) 185, 335
AluBI AGCT 8 cut(s) 77, 314, 880, 907, 961, 986, 1008, 1086
AluI AGCT 8 cut(s) 77, 314, 880, 907, 961, 986, 1008, 1086
Alw21I GWGCWC 1 cut(s) 963
AlwI GGATC 4 cut(s) 76, 89, 585, 598
AlwNI CAGNNNCTG 2 cut(s) 230, 314
Ama87I CYCGRG 1 cut(s) 472
ApaI GGGCCC 3 cut(s) 443, 479, 534
AspLEI GCGC 3 cut(s) 466, 893, 1114
AsuC2I CCSGG 4 cut(s) 473, 474, 619, 1147
AsuHPI GGTGA 8 cut(s) 267, 286, 336, 381, 625, 778, 916, 1144
AvaI CYCGRG 1 cut(s) 472
AvaII GGWCC 3 cut(s) 693, 1143, 1201
AxyI CCTNAGG 1 cut(s) 369
BaeGI GKGCMC 4 cut(s) 443, 479, 534, 776
BalI TGGCCA 3 cut(s) 165, 641, 1004
BamHI GGATCC 2 cut(s) 81, 590
BanI GGYRCC 6 cut(s) 214, 304, 373, 773, 890, 1111
BanII GRGCYC 5 cut(s) 443, 479, 534, 624, 963
Bbv12I GWGCWC 1 cut(s) 963
BbvCI CCTCAGC 1 cut(s) 210
BccI CCATC 4 cut(s) 56, 491, 764, 1088
BceAI ACGGC 5 cut(s) 736, 859, 904, 956, 1072
BciT130I CCWGG 1 cut(s) 122
BciVI GTATCC 1 cut(s) 1219
BcnI CCSGG 4 cut(s) 473, 474, 619, 1147
BfaI CTAG 3 cut(s) 44, 78, 977
BfoI RGCGCY 2 cut(s) 894, 1115
BfuI GTATCC 1 cut(s) 1219
BglI GCCNNNNNGGC 1 cut(s) 771
BisI GCNGC 3 cut(s) 503, 847, 1110
BlsI GCNGC 3 cut(s) 504, 848, 1111
Bme1390I CCNGG 5 cut(s) 122, 473, 474, 619, 1147
Bme18I GGWCC 3 cut(s) 693, 1143, 1201
BmeT110I CYCGRG 1 cut(s) 472
BmgBI CACGTC 1 cut(s) 1069
BmrFI CCNGG 5 cut(s) 122, 473, 474, 619, 1147
BmrI ACTGGG 1 cut(s) 503
BmsI GCATC 3 cut(s) 223, 281, 350
BmuI ACTGGG 1 cut(s) 503
BoxI GACNNNNGTC 1 cut(s) 116
BplI GAGNNNNNCTC 2 cut(s) 950, 982
BpmI CTGGAG 1 cut(s) 884
Bpu10I CCTNAGC 2 cut(s) 210, 987
BpuMI CCSGG 4 cut(s) 473, 474, 619, 1147
BsaHI GRCGYC 2 cut(s) 891, 1112
BsaJI CCNNGG 4 cut(s) 186, 300, 472, 522
BsaXI ACNNNNNCTCC 2 cut(s) 737, 767
Bsc4I CCNNNNNNNGG 8 cut(s) 437, 473, 527, 528, 686, 1033, 1108, 1195
Bse118I RCCGGY 4 cut(s) 761, 779, 872, 1114
Bse1I ACTGG 1 cut(s) 509
Bse21I CCTNAGG 1 cut(s) 369
BseBI CCWGG 1 cut(s) 122
BseDI CCNNGG 4 cut(s) 186, 300, 472, 522
BseGI GGATG 4 cut(s) 196, 447, 483, 810
BseLI CCNNNNNNNGG 8 cut(s) 437, 473, 527, 528, 686, 1033, 1108, 1195
BseMII CTCAG 5 cut(s) 87, 201, 360, 1001, 1248
BseNI ACTGG 1 cut(s) 509
BseSI GKGCMC 4 cut(s) 443, 479, 534, 776
BseYI CCCAGC 1 cut(s) 478
Bsh1236I CGCG 2 cut(s) 302, 464
BshNI GGYRCC 6 cut(s) 214, 304, 373, 773, 890, 1111
BsiHKAI GWGCWC 1 cut(s) 963
BsiHKCI CYCGRG 1 cut(s) 472
BsiSI CCGG 8 cut(s) 473, 618, 762, 780, 822, 873, 1115, 1146
BslFI GGGAC 2 cut(s) 706, 1156
BslI CCNNNNNNNGG 8 cut(s) 437, 473, 527, 528, 686, 1033, 1108, 1195
BsmFI GGGAC 2 cut(s) 706, 1156
BsmI GAATGC 1 cut(s) 1165
BsoBI CYCGRG 1 cut(s) 472
Bsp120I GGGCCC 3 cut(s) 439, 475, 530
Bsp1286I GDGCHC 6 cut(s) 443, 479, 534, 624, 776, 963
Bsp143I GATC 4 cut(s) 81, 590, 1134, 1239
BspCNI CTCAG 5 cut(s) 86, 202, 361, 1000, 1249
BspFNI CGCG 2 cut(s) 302, 464
BspPI GGATC 4 cut(s) 76, 89, 585, 598
BspT107I GGYRCC 6 cut(s) 214, 304, 373, 773, 890, 1111
BsrBI CCGCTC 1 cut(s) 505
BsrFI RCCGGY 4 cut(s) 761, 779, 872, 1114
BsrI ACTGG 1 cut(s) 509
BssAI RCCGGY 4 cut(s) 761, 779, 872, 1114
BssECI CCNNGG 4 cut(s) 186, 300, 472, 522
BssMI GATC 4 cut(s) 81, 590, 1134, 1239
BssNI GRCGYC 2 cut(s) 891, 1112
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 8 cut(s) 118, 187, 600, 637, 808, 928, 1045, 1220
BstACI GRCGYC 2 cut(s) 891, 1112
BstC8I GCNNGC 8 cut(s) 439, 534, 631, 763, 874, 878, 1006, 1061
BstDEI CTNAG 5 cut(s) 73, 210, 369, 987, 1257
BstDSI CCRYGG 3 cut(s) 186, 300, 522
BstEII GGTNACC 1 cut(s) 613
BstENI CCTNNNNNAGG 1 cut(s) 1193
BstF5I GGATG 4 cut(s) 196, 447, 483, 810
BstFNI CGCG 2 cut(s) 302, 464
BstH2I RGCGCY 2 cut(s) 894, 1115
BstHHI GCGC 3 cut(s) 466, 893, 1114
BstKTI GATC 4 cut(s) 84, 593, 1137, 1242
BstMBI GATC 4 cut(s) 81, 590, 1134, 1239
BstMWI GCNNNNNNNGC 8 cut(s) 20, 74, 533, 647, 771, 780, 1060, 1120
BstNI CCWGG 1 cut(s) 122
BstPAI GACNNNNGTC 1 cut(s) 116
BstPI GGTNACC 1 cut(s) 613
BstSCI CCNGG 5 cut(s) 120, 471, 472, 617, 1145
BstSLI GKGCMC 4 cut(s) 443, 479, 534, 776
BstUI CGCG 2 cut(s) 302, 464
BstX2I RGATCY 2 cut(s) 81, 590
BstXI CCANNNNNNTGG 4 cut(s) 187, 337, 637, 1012
BstYI RGATCY 2 cut(s) 81, 590
Bsu36I CCTNAGG 1 cut(s) 369
BsuI GTATCC 1 cut(s) 1219
BtgI CCRYGG 3 cut(s) 186, 300, 522
BtgZI GCGATG 2 cut(s) 610, 742
BtrI CACGTC 1 cut(s) 1069
BtsCI GGATG 4 cut(s) 196, 447, 483, 810
BtsI GCAGTG 1 cut(s) 1104
BtsIMutI CAGTG 6 cut(s) 228, 330, 804, 1041, 1104, 1249
Cac8I GCNNGC 8 cut(s) 439, 534, 631, 763, 874, 878, 1006, 1061
CaiI CAGNNNCTG 2 cut(s) 230, 314
CfoI GCGC 3 cut(s) 466, 893, 1114
Cfr10I RCCGGY 4 cut(s) 761, 779, 872, 1114
Cfr42I CCGCGG 1 cut(s) 303
Cfr9I CCCGGG 1 cut(s) 472
CseI GACGC 1 cut(s) 215
Csp6I GTAC 1 cut(s) 1097
CspCI CAANNNNNGTGG 2 cut(s) 504, 539
CviAII CATG 4 cut(s) 571, 910, 1051, 1064
CviQI GTAC 1 cut(s) 1097
DdeI CTNAG 5 cut(s) 73, 210, 369, 987, 1257
DinI GGCGCC 2 cut(s) 892, 1113
DpnI GATC 4 cut(s) 83, 592, 1136, 1241
DpnII GATC 4 cut(s) 81, 590, 1134, 1239
EaeI YGGCCR 4 cut(s) 163, 639, 874, 1002
EciI GGCGGA 1 cut(s) 958
Ecl136II GAGCTC 1 cut(s) 961
Eco24I GRGCYC 5 cut(s) 443, 479, 534, 624, 963
Eco47I GGWCC 3 cut(s) 693, 1143, 1201
Eco53kI GAGCTC 1 cut(s) 961
Eco81I CCTNAGG 1 cut(s) 369
Eco88I CYCGRG 1 cut(s) 472
Eco91I GGTNACC 1 cut(s) 613
EcoICRI GAGCTC 1 cut(s) 961
EcoNI CCTNNNNNAGG 1 cut(s) 1193
EcoO109I RGGNCCY 3 cut(s) 440, 530, 693
EcoO65I GGTNACC 1 cut(s) 613
EcoRII CCWGG 1 cut(s) 120
EcoT38I GRGCYC 5 cut(s) 443, 479, 534, 624, 963
EgeI GGCGCC 2 cut(s) 892, 1113
EheI GGCGCC 2 cut(s) 892, 1113
FaeI CATG 4 cut(s) 574, 913, 1054, 1067
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 2 cut(s) 706, 1156
FatI CATG 4 cut(s) 570, 909, 1050, 1063
FauI CCCGC 5 cut(s) 307, 430, 520, 541, 1133
Fnu4HI GCNGC 3 cut(s) 503, 847, 1110
FokI GGATG 4 cut(s) 203, 434, 470, 797
FriOI GRGCYC 5 cut(s) 443, 479, 534, 624, 963
Fsp4HI GCNGC 3 cut(s) 503, 847, 1110
FspBI CTAG 3 cut(s) 44, 78, 977
GlaI GCGC 3 cut(s) 465, 892, 1113
GluI GCNGC 3 cut(s) 503, 847, 1110
GsaI CCCAGC 1 cut(s) 482
GsuI CTGGAG 1 cut(s) 884
HaeII RGCGCY 2 cut(s) 894, 1115
HapII CCGG 8 cut(s) 473, 618, 762, 780, 822, 873, 1115, 1146
HgaI GACGC 1 cut(s) 215
HhaI GCGC 3 cut(s) 466, 893, 1114
Hin1I GRCGYC 2 cut(s) 891, 1112
Hin1II CATG 4 cut(s) 574, 913, 1054, 1067
Hin6I GCGC 3 cut(s) 464, 891, 1112
HinP1I GCGC 3 cut(s) 464, 891, 1112
HincII GTYRAC 1 cut(s) 841
HindII GTYRAC 1 cut(s) 841
HinfI GANTC 1 cut(s) 386
HpaII CCGG 8 cut(s) 473, 618, 762, 780, 822, 873, 1115, 1146
HphI GGTGA 8 cut(s) 267, 286, 336, 381, 625, 778, 916, 1144
Hpy166II GTNNAC 4 cut(s) 841, 1041, 1099, 1249
Hpy188I TCNGA 1 cut(s) 1258
Hpy188III TCNNGA 6 cut(s) 109, 224, 487, 655, 901, 1196
Hpy8I GTNNAC 4 cut(s) 841, 1041, 1099, 1249
Hpy99I CGWCG 3 cut(s) 209, 650, 938
HpyAV CCTTC 6 cut(s) 37, 574, 661, 871, 944, 1199
HpyCH4III ACNGT 8 cut(s) 118, 187, 600, 637, 808, 928, 1045, 1220
HpyCH4IV ACGT 2 cut(s) 645, 1068
HpyCH4V TGCA 4 cut(s) 515, 574, 1054, 1237
HpyF10VI GCNNNNNNNGC 8 cut(s) 20, 74, 533, 647, 771, 780, 1060, 1120
HpyF3I CTNAG 5 cut(s) 73, 210, 369, 987, 1257
HpySE526I ACGT 2 cut(s) 645, 1068
Hsp92I GRCGYC 2 cut(s) 891, 1112
Hsp92II CATG 4 cut(s) 574, 913, 1054, 1067
HspAI GCGC 3 cut(s) 464, 891, 1112
KasI GGCGCC 2 cut(s) 890, 1111
KflI GGGWCCC 1 cut(s) 693
KroI GCCGGC 2 cut(s) 761, 872
KroNI GCCGGC 2 cut(s) 763, 874
KspI CCGCGG 1 cut(s) 303
Kzo9I GATC 4 cut(s) 81, 590, 1134, 1239
LmnI GCTCC 6 cut(s) 361, 510, 885, 923, 958, 971
LweI GCATC 3 cut(s) 223, 281, 350
MaeI CTAG 3 cut(s) 44, 78, 977
MaeII ACGT 2 cut(s) 645, 1068
MaeIII GTNAC 8 cut(s) 250, 283, 292, 319, 361, 613, 865, 922
MalI GATC 4 cut(s) 83, 592, 1136, 1241
MbiI CCGCTC 1 cut(s) 505
MboI GATC 4 cut(s) 81, 590, 1134, 1239
MboII GAAGA 4 cut(s) 413, 559, 667, 1081
MflI RGATCY 2 cut(s) 81, 590
MhlI GDGCHC 6 cut(s) 443, 479, 534, 624, 776, 963
MlsI TGGCCA 3 cut(s) 165, 641, 1004
MluCI AATT 1 cut(s) 219
MluNI TGGCCA 3 cut(s) 165, 641, 1004
Mly113I GGCGCC 2 cut(s) 891, 1112
MlyI GAGTC 1 cut(s) 380
Mox20I TGGCCA 3 cut(s) 165, 641, 1004
MroNI GCCGGC 2 cut(s) 761, 872
MscI TGGCCA 3 cut(s) 165, 641, 1004
MslI CAYNNNNRTG 2 cut(s) 185, 335
Msp20I TGGCCA 3 cut(s) 165, 641, 1004
MspA1I CMGCKG 2 cut(s) 302, 687
MspI CCGG 8 cut(s) 473, 618, 762, 780, 822, 873, 1115, 1146
MspR9I CCNGG 5 cut(s) 122, 473, 474, 619, 1147
Mva1269I GAATGC 1 cut(s) 1165
MvaI CCWGG 1 cut(s) 122
MvnI CGCG 2 cut(s) 302, 464
MwoI GCNNNNNNNGC 8 cut(s) 20, 74, 533, 647, 771, 780, 1060, 1120
NaeI GCCGGC 2 cut(s) 763, 874
NarI GGCGCC 2 cut(s) 891, 1112
NciI CCSGG 4 cut(s) 473, 474, 619, 1147
NdeII GATC 4 cut(s) 81, 590, 1134, 1239
NgoMIV GCCGGC 2 cut(s) 761, 872
NlaIII CATG 4 cut(s) 574, 913, 1054, 1067
NmeAIII GCCGAG 1 cut(s) 942
NmuCI GTSAC 6 cut(s) 250, 292, 319, 613, 865, 922
OliI CACNNNNGTG 2 cut(s) 185, 335
PcsI WCGNNNNNNNCGW 2 cut(s) 726, 969
PctI GAATGC 1 cut(s) 1165
PdiI GCCGGC 2 cut(s) 763, 874
PflMI CCANNNNNTGG 1 cut(s) 1033
PkrI GCNGC 3 cut(s) 504, 848, 1111
PleI GAGTC 1 cut(s) 380
PluTI GGCGCC 2 cut(s) 894, 1115
PpsI GAGTC 1 cut(s) 380
PpuMI RGGWCCY 1 cut(s) 693
PshAI GACNNNNGTC 1 cut(s) 116
Psp124BI GAGCTC 1 cut(s) 963
Psp5II RGGWCCY 1 cut(s) 693
Psp6I CCWGG 1 cut(s) 120
PspEI GGTNACC 1 cut(s) 613
PspFI CCCAGC 1 cut(s) 478
PspGI CCWGG 1 cut(s) 120
PspOMI GGGCCC 3 cut(s) 439, 475, 530
PspPPI RGGWCCY 1 cut(s) 693
PstNI CAGNNNCTG 2 cut(s) 230, 314
PsuI RGATCY 2 cut(s) 81, 590
RsaI GTAC 1 cut(s) 1098
RsaNI GTAC 1 cut(s) 1097
RseI CAYNNNNRTG 2 cut(s) 185, 335
SacI GAGCTC 1 cut(s) 963
SacII CCGCGG 1 cut(s) 303
SatI GCNGC 3 cut(s) 503, 847, 1110
Sau3AI GATC 4 cut(s) 81, 590, 1134, 1239
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 5 cut(s) 122, 473, 474, 619, 1147
SduI GDGCHC 6 cut(s) 443, 479, 534, 624, 776, 963
SfaNI GCATC 3 cut(s) 223, 281, 350
SfoI GGCGCC 2 cut(s) 892, 1113
Sfr303I CCGCGG 1 cut(s) 303
SgrAI CRCCGGYG 1 cut(s) 1114
SgrBI CCGCGG 1 cut(s) 303
SinI GGWCC 3 cut(s) 693, 1143, 1201
SmaI CCCGGG 1 cut(s) 474
SmiMI CAYNNNNRTG 2 cut(s) 185, 335
Sse9I AATT 1 cut(s) 219
SspDI GGCGCC 2 cut(s) 890, 1111
SspMI CTAG 3 cut(s) 44, 78, 977
SstI GAGCTC 1 cut(s) 963
StyD4I CCNGG 5 cut(s) 120, 471, 472, 617, 1145
TaaI ACNGT 8 cut(s) 118, 187, 600, 637, 808, 928, 1045, 1220
TaiI ACGT 2 cut(s) 648, 1071
TaqI TCGA 3 cut(s) 656, 729, 933
TaqII GACCGA 1 cut(s) 962
TasI AATT 1 cut(s) 219
TauI GCSGC 3 cut(s) 505, 849, 1112
TscAI CASTG 6 cut(s) 235, 337, 811, 1048, 1111, 1249
TseFI GTSAC 6 cut(s) 250, 292, 319, 613, 865, 922
Tsp45I GTSAC 6 cut(s) 250, 292, 319, 613, 865, 922
TspDTI ATGAA 3 cut(s) 105, 559, 1181
TspGWI ACGGA 5 cut(s) 141, 592, 910, 1032, 1229
TspMI CCCGGG 1 cut(s) 472
TspRI CASTG 6 cut(s) 235, 337, 811, 1048, 1111, 1249
Van91I CCANNNNNTGG 1 cut(s) 1033
VpaK11BI GGWCC 3 cut(s) 693, 1143, 1201
XagI CCTNNNNNAGG 1 cut(s) 1193
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XmaI CCCGGG 1 cut(s) 472
XspI CTAG 3 cut(s) 44, 78, 977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.