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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G03820 |
| fragaria_vesca | FvH4_2g19240 FvH4_6g18310 FvH4_6g26650 |
| malus_domestica | MD09G1241900.v1.1 |
| prunus_persica | Prupe.3G134900_v2.0.a1 |
| pyrus_communis | pycom09g16050 |
| rosa_laevigata | RLG00000018973 RLG00000024057 |
| rosa_multiflora | Rmu_sc0005608.1_g000022 Rmu_ssc0000018.1_g000064 |
| rosa_roxburghii | Rroxscaffold_2G00116280 Rroxscaffold_6G00408620 |
| rosa_rugosa | Rorug02G0276200 Rorug03G0127800 |
| rosa_samantha | Rh2AG327000 Rh2BG337300 Rh2BG337400 Rh2CG314400 Rh2DG354400 Rh3BG206300 Rh3CG203400 Rh3CG221700 Rh3DG169400 |
| rosa_wichuraiana | Rw0G004980 Rw2G026520 Rw2G026560 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 2 cut(s) | 1152, 3077 |
| Acc36I | ACCTGC | 2 cut(s) | 1520, 1932 |
| Acc65I | GGTACC | 1 cut(s) | 3061 |
| AccB1I | GGYRCC | 5 cut(s) | 2265, 2373, 3061, 3182, 3217 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2732 |
| AccBSI | CCGCTC | 1 cut(s) | 406 |
| AccI | GTMKAC | 2 cut(s) | 1705, 2000 |
| AciI | CCGC | 8 cut(s) | 77, 305, 406, 623, 862, 1957, 2390, 2758 |
| AclI | AACGTT | 1 cut(s) | 1627 |
| AclWI | GGATC | 7 cut(s) | 368, 381, 815, 1652, 2462, 2842, 3044 |
| AcoI | YGGCCR | 2 cut(s) | 466, 1598 |
| AcuI | CTGAAG | 2 cut(s) | 1790, 2166 |
| AcvI | CACGTG | 1 cut(s) | 1604 |
| AcyI | GRCGYC | 1 cut(s) | 3070 |
| AdeI | CACNNNGTG | 1 cut(s) | 250 |
| AfaI | GTAC | 5 cut(s) | 811, 986, 1428, 2191, 3063 |
| AflII | CTTAAG | 1 cut(s) | 3056 |
| AflIII | ACRYGT | 1 cut(s) | 1204 |
| AgeI | ACCGGT | 1 cut(s) | 2164 |
| AjnI | CCWGG | 7 cut(s) | 11, 135, 468, 1419, 1688, 2781, 2976 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2434, 2466 |
| Alw21I | GWGCWC | 3 cut(s) | 514, 1502, 2938 |
| Alw26I | GTCTC | 6 cut(s) | 1409, 1895, 2489, 2545, 2615, 2754 |
| AlwI | GGATC | 7 cut(s) | 368, 381, 815, 1652, 2462, 2842, 3044 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 2435, 2438, 3161, 3229 |
| ApaI | GGGCCC | 1 cut(s) | 2362 |
| AseI | ATTAAT | 2 cut(s) | 2690, 3045 |
| AsiGI | ACCGGT | 1 cut(s) | 2164 |
| Asp718I | GGTACC | 1 cut(s) | 3061 |
| AspA2I | CCTAGG | 1 cut(s) | 2237 |
| AspLEI | GCGC | 4 cut(s) | 1153, 1170, 1609, 3078 |
| AvaII | GGWCC | 4 cut(s) | 104, 1081, 1120, 2793 |
| AvrII | CCTAGG | 1 cut(s) | 2237 |
| AxyI | CCTNAGG | 1 cut(s) | 2106 |
| BaeGI | GKGCMC | 1 cut(s) | 2362 |
| BalI | TGGCCA | 2 cut(s) | 468, 1600 |
| BamHI | GGATCC | 1 cut(s) | 373 |
| BanI | GGYRCC | 5 cut(s) | 2265, 2373, 3061, 3182, 3217 |
| BanII | GRGCYC | 3 cut(s) | 1502, 2362, 2640 |
| BbrPI | CACGTG | 1 cut(s) | 1604 |
| BbsI | GAAGAC | 1 cut(s) | 138 |
| Bbv12I | GWGCWC | 3 cut(s) | 514, 1502, 2938 |
| BceAI | ACGGC | 2 cut(s) | 2403, 2781 |
| BciT130I | CCWGG | 7 cut(s) | 13, 137, 470, 1421, 1690, 2783, 2978 |
| BciVI | GTATCC | 1 cut(s) | 212 |
| BclI | TGATCA | 2 cut(s) | 190, 2845 |
| BcoDI | GTCTC | 6 cut(s) | 1409, 1895, 2489, 2545, 2615, 2754 |
| BfaI | CTAG | 4 cut(s) | 704, 2238, 2685, 3108 |
| BfmI | CTRYAG | 5 cut(s) | 108, 1238, 1538, 1702, 2641 |
| BfrI | CTTAAG | 1 cut(s) | 3056 |
| BfuAI | ACCTGC | 2 cut(s) | 1520, 1932 |
| BfuI | GTATCC | 1 cut(s) | 212 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 332 |
| BlnI | CCTAGG | 1 cut(s) | 2237 |
| Bme1390I | CCNGG | 7 cut(s) | 13, 137, 470, 1421, 1690, 2783, 2978 |
| Bme18I | GGWCC | 4 cut(s) | 104, 1081, 1120, 2793 |
| BmrFI | CCNGG | 7 cut(s) | 13, 137, 470, 1421, 1690, 2783, 2978 |
| BmsI | GCATC | 6 cut(s) | 64, 307, 307, 1179, 1863, 2416 |
| BpiI | GAAGAC | 1 cut(s) | 138 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 3005, 3037 |
| BpmI | CTGGAG | 2 cut(s) | 34, 683 |
| BpuEI | CTTGAG | 2 cut(s) | 1877, 2597 |
| Bsa29I | ATCGAT | 1 cut(s) | 278 |
| BsaAI | YACGTR | 1 cut(s) | 1604 |
| BsaHI | GRCGYC | 1 cut(s) | 3070 |
| BsaI | GGTCTC | 2 cut(s) | 1895, 2489 |
| BsaJI | CCNNGG | 5 cut(s) | 1420, 2081, 2202, 2237, 3065 |
| BsaWI | WCCGGW | 1 cut(s) | 2164 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 3079, 3109, 3186, 3216 |
| Bse118I | RCCGGY | 1 cut(s) | 2164 |
| Bse1I | ACTGG | 3 cut(s) | 132, 469, 2499 |
| Bse21I | CCTNAGG | 1 cut(s) | 2106 |
| Bse3DI | GCAATG | 3 cut(s) | 1470, 1849, 1934 |
| BseBI | CCWGG | 7 cut(s) | 13, 137, 470, 1421, 1690, 2783, 2978 |
| BseCI | ATCGAT | 1 cut(s) | 278 |
| BseDI | CCNNGG | 5 cut(s) | 1420, 2081, 2202, 2237, 3065 |
| BseGI | GGATG | 5 cut(s) | 40, 505, 1675, 1948, 2702 |
| BseMI | GCAATG | 3 cut(s) | 1470, 1849, 1934 |
| BseMII | CTCAG | 8 cut(s) | 420, 852, 1115, 1188, 2097, 2589, 3049, 3236 |
| BseNI | ACTGG | 3 cut(s) | 132, 469, 2499 |
| BseRI | GAGGAG | 6 cut(s) | 101, 680, 1130, 1298, 2793, 2796 |
| BseSI | GKGCMC | 1 cut(s) | 2362 |
| BseYI | CCCAGC | 2 cut(s) | 1978, 2314 |
| BsgI | GTGCAG | 1 cut(s) | 2173 |
| BshNI | GGYRCC | 5 cut(s) | 2265, 2373, 3061, 3182, 3217 |
| BshTI | ACCGGT | 1 cut(s) | 2164 |
| BshVI | ATCGAT | 1 cut(s) | 278 |
| BsiHKAI | GWGCWC | 3 cut(s) | 514, 1502, 2938 |
| BsiSI | CCGG | 2 cut(s) | 19, 2165 |
| BslFI | GGGAC | 5 cut(s) | 181, 236, 1094, 2779, 2912 |
| BsmAI | GTCTC | 6 cut(s) | 1409, 1895, 2489, 2545, 2615, 2754 |
| BsmFI | GGGAC | 5 cut(s) | 181, 236, 1094, 2779, 2912 |
| BsmI | GAATGC | 1 cut(s) | 3232 |
| Bso31I | GGTCTC | 2 cut(s) | 1895, 2489 |
| Bsp120I | GGGCCC | 1 cut(s) | 2358 |
| Bsp1286I | GDGCHC | 5 cut(s) | 514, 1502, 2362, 2640, 2938 |
| Bsp19I | CCATGG | 1 cut(s) | 2081 |
| BspACI | CCGC | 8 cut(s) | 77, 305, 406, 623, 862, 1957, 2390, 2758 |
| BspCNI | CTCAG | 8 cut(s) | 421, 851, 1116, 1187, 2098, 2590, 3048, 3235 |
| BspDI | ATCGAT | 1 cut(s) | 278 |
| BspHI | TCATGA | 1 cut(s) | 2512 |
| BspMAI | CTGCAG | 1 cut(s) | 1542 |
| BspMI | ACCTGC | 2 cut(s) | 1520, 1932 |
| BspPI | GGATC | 7 cut(s) | 368, 381, 815, 1652, 2462, 2842, 3044 |
| BspT107I | GGYRCC | 5 cut(s) | 2265, 2373, 3061, 3182, 3217 |
| BspTI | CTTAAG | 1 cut(s) | 3056 |
| BspTNI | GGTCTC | 2 cut(s) | 1895, 2489 |
| BsrBI | CCGCTC | 1 cut(s) | 406 |
| BsrDI | GCAATG | 3 cut(s) | 1470, 1849, 1934 |
| BsrFI | RCCGGY | 1 cut(s) | 2164 |
| BsrI | ACTGG | 3 cut(s) | 132, 469, 2499 |
| BssAI | RCCGGY | 1 cut(s) | 2164 |
| BssECI | CCNNGG | 5 cut(s) | 1420, 2081, 2202, 2237, 3065 |
| BssNI | GRCGYC | 1 cut(s) | 3070 |
| BssT1I | CCWWGG | 4 cut(s) | 2081, 2202, 2237, 3065 |
| Bst2UI | CCWGG | 7 cut(s) | 13, 137, 470, 1421, 1690, 2783, 2978 |
| Bst4CI | ACNGT | 7 cut(s) | 487, 1058, 1614, 2426, 2843, 3095, 3114 |
| Bst6I | CTCTTC | 2 cut(s) | 1766, 2142 |
| BstACI | GRCGYC | 1 cut(s) | 3070 |
| BstAFI | CTTAAG | 1 cut(s) | 3056 |
| BstBAI | YACGTR | 1 cut(s) | 1604 |
| BstC8I | GCNNGC | 4 cut(s) | 174, 557, 2244, 3171 |
| BstDSI | CCRYGG | 1 cut(s) | 2081 |
| BstEII | GGTNACC | 1 cut(s) | 1800 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 367, 1112 |
| BstF5I | GGATG | 5 cut(s) | 40, 505, 1675, 1948, 2702 |
| BstHHI | GCGC | 4 cut(s) | 1153, 1170, 1609, 3078 |
| BstMAI | GTCTC | 6 cut(s) | 1409, 1895, 2489, 2545, 2615, 2754 |
| BstNI | CCWGG | 7 cut(s) | 13, 137, 470, 1421, 1690, 2783, 2978 |
| BstNSI | RCATGY | 1 cut(s) | 1208 |
| BstPI | GGTNACC | 1 cut(s) | 1800 |
| BstSCI | CCNGG | 7 cut(s) | 11, 135, 468, 1419, 1688, 2781, 2976 |
| BstSFI | CTRYAG | 5 cut(s) | 108, 1238, 1538, 1702, 2641 |
| BstSLI | GKGCMC | 1 cut(s) | 2362 |
| BstV2I | GAAGAC | 1 cut(s) | 138 |
| BstX2I | RGATCY | 3 cut(s) | 373, 1657, 2454 |
| BstYI | RGATCY | 3 cut(s) | 373, 1657, 2454 |
| Bsu15I | ATCGAT | 1 cut(s) | 278 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2106 |
| BsuI | GTATCC | 1 cut(s) | 212 |
| BsuTUI | ATCGAT | 1 cut(s) | 278 |
| BtgI | CCRYGG | 1 cut(s) | 2081 |
| BtsCI | GGATG | 5 cut(s) | 40, 505, 1675, 1948, 2702 |
| BtsI | GCAGTG | 1 cut(s) | 2161 |
| BtsIMutI | CAGTG | 7 cut(s) | 125, 514, 846, 1030, 2161, 2506, 3162 |
| BveI | ACCTGC | 2 cut(s) | 1520, 1932 |
| Cac8I | GCNNGC | 4 cut(s) | 174, 557, 2244, 3171 |
| CaiI | CAGNNNCTG | 4 cut(s) | 2435, 2438, 3161, 3229 |
| CciI | TCATGA | 1 cut(s) | 2512 |
| CfoI | GCGC | 4 cut(s) | 1153, 1170, 1609, 3078 |
| Cfr10I | RCCGGY | 1 cut(s) | 2164 |
| ClaI | ATCGAT | 1 cut(s) | 278 |
| CseI | GACGC | 3 cut(s) | 273, 2951, 3059 |
| Csp6I | GTAC | 5 cut(s) | 810, 985, 1427, 2190, 3062 |
| CspAI | ACCGGT | 1 cut(s) | 2164 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1024, 1059 |
| CviQI | GTAC | 5 cut(s) | 810, 985, 1427, 2190, 3062 |
| DraI | TTTAAA | 4 cut(s) | 721, 1260, 2650, 2988 |
| DraIII | CACNNNGTG | 1 cut(s) | 250 |
| EaeI | YGGCCR | 2 cut(s) | 466, 1598 |
| Eam1104I | CTCTTC | 2 cut(s) | 1766, 2142 |
| EarI | CTCTTC | 2 cut(s) | 1766, 2142 |
| Ecl136II | GAGCTC | 1 cut(s) | 1500 |
| Eco130I | CCWWGG | 4 cut(s) | 2081, 2202, 2237, 3065 |
| Eco147I | AGGCCT | 2 cut(s) | 694, 2891 |
| Eco24I | GRGCYC | 3 cut(s) | 1502, 2362, 2640 |
| Eco31I | GGTCTC | 2 cut(s) | 1895, 2489 |
| Eco47I | GGWCC | 4 cut(s) | 104, 1081, 1120, 2793 |
| Eco53kI | GAGCTC | 1 cut(s) | 1500 |
| Eco57I | CTGAAG | 2 cut(s) | 1790, 2166 |
| Eco72I | CACGTG | 1 cut(s) | 1604 |
| Eco81I | CCTNAGG | 1 cut(s) | 2106 |
| Eco91I | GGTNACC | 1 cut(s) | 1800 |
| EcoICRI | GAGCTC | 1 cut(s) | 1500 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 367, 1112 |
| EcoO109I | RGGNCCY | 6 cut(s) | 518, 569, 1081, 1120, 2358, 2359 |
| EcoO65I | GGTNACC | 1 cut(s) | 1800 |
| EcoRI | GAATTC | 3 cut(s) | 1297, 1380, 3151 |
| EcoRII | CCWGG | 7 cut(s) | 11, 135, 468, 1419, 1688, 2781, 2976 |
| EcoT14I | CCWWGG | 4 cut(s) | 2081, 2202, 2237, 3065 |
| EcoT38I | GRGCYC | 3 cut(s) | 1502, 2362, 2640 |
| ErhI | CCWWGG | 4 cut(s) | 2081, 2202, 2237, 3065 |
| FaqI | GGGAC | 5 cut(s) | 181, 236, 1094, 2779, 2912 |
| FbaI | TGATCA | 2 cut(s) | 190, 2845 |
| FblI | GTMKAC | 2 cut(s) | 1705, 2000 |
| FokI | GGATG | 5 cut(s) | 47, 512, 1682, 1955, 2689 |
| FriOI | GRGCYC | 3 cut(s) | 1502, 2362, 2640 |
| FspBI | CTAG | 4 cut(s) | 704, 2238, 2685, 3108 |
| FspI | TGCGCA | 2 cut(s) | 1152, 3077 |
| GlaI | GCGC | 4 cut(s) | 1152, 1169, 1608, 3077 |
| GsaI | CCCAGC | 2 cut(s) | 1982, 2318 |
| GsuI | CTGGAG | 2 cut(s) | 34, 683 |
| HapII | CCGG | 2 cut(s) | 19, 2165 |
| HgaI | GACGC | 3 cut(s) | 273, 2951, 3059 |
| HhaI | GCGC | 4 cut(s) | 1153, 1170, 1609, 3078 |
| Hin1I | GRCGYC | 1 cut(s) | 3070 |
| Hin6I | GCGC | 4 cut(s) | 1151, 1168, 1607, 3076 |
| HinP1I | GCGC | 4 cut(s) | 1151, 1168, 1607, 3076 |
| HindIII | AAGCTT | 2 cut(s) | 54, 3167 |
| HpaII | CCGG | 2 cut(s) | 19, 2165 |
| Hpy166II | GTNNAC | 7 cut(s) | 388, 1061, 1315, 1706, 2001, 2422, 3019 |
| Hpy8I | GTNNAC | 7 cut(s) | 388, 1061, 1315, 1706, 2001, 2422, 3019 |
| Hpy99I | CGWCG | 1 cut(s) | 1371 |
| HpyCH4III | ACNGT | 7 cut(s) | 487, 1058, 1614, 2426, 2843, 3095, 3114 |
| HpyCH4IV | ACGT | 4 cut(s) | 987, 1366, 1603, 1627 |
| HpySE526I | ACGT | 4 cut(s) | 987, 1366, 1603, 1627 |
| Hsp92I | GRCGYC | 1 cut(s) | 3070 |
| HspAI | GCGC | 4 cut(s) | 1151, 1168, 1607, 3076 |
| KflI | GGGWCCC | 1 cut(s) | 1081 |
| KpnI | GGTACC | 1 cut(s) | 3065 |
| Ksp22I | TGATCA | 2 cut(s) | 190, 2845 |
| LmnI | GCTCC | 4 cut(s) | 15, 403, 930, 2773 |
| LweI | GCATC | 6 cut(s) | 64, 307, 307, 1179, 1863, 2416 |
| MaeI | CTAG | 4 cut(s) | 704, 2238, 2685, 3108 |
| MaeII | ACGT | 4 cut(s) | 987, 1366, 1603, 1627 |
| MaeIII | GTNAC | 5 cut(s) | 424, 1189, 1565, 1800, 2009 |
| MbiI | CCGCTC | 1 cut(s) | 406 |
| MboII | GAAGA | 7 cut(s) | 143, 233, 1542, 1727, 1783, 2159, 2449 |
| MfeI | CAATTG | 2 cut(s) | 2005, 2670 |
| MflI | RGATCY | 3 cut(s) | 373, 1657, 2454 |
| MhlI | GDGCHC | 5 cut(s) | 514, 1502, 2362, 2640, 2938 |
| MlsI | TGGCCA | 2 cut(s) | 468, 1600 |
| MluNI | TGGCCA | 2 cut(s) | 468, 1600 |
| MlyI | GAGTC | 5 cut(s) | 677, 1077, 1627, 2006, 3014 |
| MmeI | TCCRAC | 3 cut(s) | 370, 1610, 2351 |
| Mox20I | TGGCCA | 2 cut(s) | 468, 1600 |
| MscI | TGGCCA | 2 cut(s) | 468, 1600 |
| MslI | CAYNNNNRTG | 1 cut(s) | 395 |
| Msp20I | TGGCCA | 2 cut(s) | 468, 1600 |
| MspA1I | CMGCKG | 2 cut(s) | 1543, 2435 |
| MspCI | CTTAAG | 1 cut(s) | 3056 |
| MspI | CCGG | 2 cut(s) | 19, 2165 |
| MspR9I | CCNGG | 7 cut(s) | 13, 137, 470, 1421, 1690, 2783, 2978 |
| MssI | GTTTAAAC | 1 cut(s) | 2650 |
| MunI | CAATTG | 2 cut(s) | 2005, 2670 |
| Mva1269I | GAATGC | 1 cut(s) | 3232 |
| MvaI | CCWGG | 7 cut(s) | 13, 137, 470, 1421, 1690, 2783, 2978 |
| NcoI | CCATGG | 1 cut(s) | 2081 |
| NmuCI | GTSAC | 4 cut(s) | 424, 1565, 1800, 2009 |
| NsbI | TGCGCA | 2 cut(s) | 1152, 3077 |
| NspI | RCATGY | 1 cut(s) | 1208 |
| PagI | TCATGA | 1 cut(s) | 2512 |
| PceI | AGGCCT | 2 cut(s) | 694, 2891 |
| PciI | ACATGT | 1 cut(s) | 1204 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1069, 1633 |
| PctI | GAATGC | 1 cut(s) | 3232 |
| PfeI | GAWTC | 8 cut(s) | 120, 1354, 1830, 2399, 2662, 2678, 2971, 3276 |
| PflFI | GACNNNGTC | 3 cut(s) | 102, 1223, 1365 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2732 |
| PfoI | TCCNGGA | 1 cut(s) | 11 |
| PinAI | ACCGGT | 1 cut(s) | 2164 |
| PleI | GAGTC | 5 cut(s) | 676, 1076, 1627, 2005, 3014 |
| PmaCI | CACGTG | 1 cut(s) | 1604 |
| PmeI | GTTTAAAC | 1 cut(s) | 2650 |
| PmlI | CACGTG | 1 cut(s) | 1604 |
| PpsI | GAGTC | 5 cut(s) | 676, 1076, 1627, 2005, 3014 |
| Ppu21I | YACGTR | 1 cut(s) | 1604 |
| PpuMI | RGGWCCY | 2 cut(s) | 1081, 1120 |
| PscI | ACATGT | 1 cut(s) | 1204 |
| PshBI | ATTAAT | 2 cut(s) | 2690, 3045 |
| Psp124BI | GAGCTC | 1 cut(s) | 1502 |
| Psp1406I | AACGTT | 1 cut(s) | 1627 |
| Psp5II | RGGWCCY | 2 cut(s) | 1081, 1120 |
| Psp6I | CCWGG | 7 cut(s) | 11, 135, 468, 1419, 1688, 2781, 2976 |
| PspCI | CACGTG | 1 cut(s) | 1604 |
| PspEI | GGTNACC | 1 cut(s) | 1800 |
| PspFI | CCCAGC | 2 cut(s) | 1978, 2314 |
| PspGI | CCWGG | 7 cut(s) | 11, 135, 468, 1419, 1688, 2781, 2976 |
| PspOMI | GGGCCC | 1 cut(s) | 2358 |
| PspPPI | RGGWCCY | 2 cut(s) | 1081, 1120 |
| PstI | CTGCAG | 1 cut(s) | 1542 |
| PstNI | CAGNNNCTG | 4 cut(s) | 2435, 2438, 3161, 3229 |
| PsuI | RGATCY | 3 cut(s) | 373, 1657, 2454 |
| PsyI | GACNNNGTC | 3 cut(s) | 102, 1223, 1365 |
| PvuII | CAGCTG | 2 cut(s) | 1543, 2435 |
| RsaI | GTAC | 5 cut(s) | 811, 986, 1428, 2191, 3063 |
| RsaNI | GTAC | 5 cut(s) | 810, 985, 1427, 2190, 3062 |
| RseI | CAYNNNNRTG | 1 cut(s) | 395 |
| SacI | GAGCTC | 1 cut(s) | 1502 |
| SchI | GAGTC | 5 cut(s) | 677, 1077, 1627, 2006, 3014 |
| ScrFI | CCNGG | 7 cut(s) | 13, 137, 470, 1421, 1690, 2783, 2978 |
| SduI | GDGCHC | 5 cut(s) | 514, 1502, 2362, 2640, 2938 |
| SfaNI | GCATC | 6 cut(s) | 64, 307, 307, 1179, 1863, 2416 |
| SfcI | CTRYAG | 5 cut(s) | 108, 1238, 1538, 1702, 2641 |
| SinI | GGWCC | 4 cut(s) | 104, 1081, 1120, 2793 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 395 |
| SmlI | CTYRAG | 3 cut(s) | 1892, 2612, 3056 |
| SmoI | CTYRAG | 3 cut(s) | 1892, 2612, 3056 |
| SseBI | AGGCCT | 2 cut(s) | 694, 2891 |
| SsiI | CCGC | 8 cut(s) | 77, 305, 406, 623, 862, 1957, 2390, 2758 |
| SspI | AATATT | 6 cut(s) | 738, 997, 1396, 1460, 2578, 3121 |
| SspMI | CTAG | 4 cut(s) | 704, 2238, 2685, 3108 |
| SstI | GAGCTC | 1 cut(s) | 1502 |
| StuI | AGGCCT | 2 cut(s) | 694, 2891 |
| StyD4I | CCNGG | 7 cut(s) | 11, 135, 468, 1419, 1688, 2781, 2976 |
| StyI | CCWWGG | 4 cut(s) | 2081, 2202, 2237, 3065 |
| TaaI | ACNGT | 7 cut(s) | 487, 1058, 1614, 2426, 2843, 3095, 3114 |
| TaiI | ACGT | 4 cut(s) | 990, 1369, 1606, 1630 |
| TaqI | TCGA | 2 cut(s) | 278, 1995 |
| TaqII | GACCGA | 1 cut(s) | 1877 |
| TauI | GCSGC | 4 cut(s) | 409, 625, 2393, 2761 |
| TfiI | GAWTC | 8 cut(s) | 120, 1354, 1830, 2399, 2662, 2678, 2971, 3276 |
| TscAI | CASTG | 7 cut(s) | 132, 514, 846, 1030, 2161, 2506, 3162 |
| TseFI | GTSAC | 4 cut(s) | 424, 1565, 1800, 2009 |
| Tsp45I | GTSAC | 4 cut(s) | 424, 1565, 1800, 2009 |
| TspDTI | ATGAA | 9 cut(s) | 29, 604, 863, 1267, 1310, 1838, 2501, 2512, 3113 |
| TspGWI | ACGGA | 2 cut(s) | 1061, 1853 |
| TspRI | CASTG | 7 cut(s) | 132, 514, 846, 1030, 2161, 2506, 3162 |
| Tth111I | GACNNNGTC | 3 cut(s) | 102, 1223, 1365 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2732 |
| Vha464I | CTTAAG | 1 cut(s) | 3056 |
| VpaK11BI | GGWCC | 4 cut(s) | 104, 1081, 1120, 2793 |
| VspI | ATTAAT | 2 cut(s) | 2690, 3045 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 367, 1112 |
| XbaI | TCTAGA | 1 cut(s) | 3107 |
| XceI | RCATGY | 1 cut(s) | 1208 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1336 |
| XmaJI | CCTAGG | 1 cut(s) | 2237 |
| XmiI | GTMKAC | 2 cut(s) | 1705, 2000 |
| XspI | CTAG | 4 cut(s) | 704, 2238, 2685, 3108 |