Rorug03G0127800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0006365
Physical Location & Seq
Reverse (-)
10335508 .. 10335888
381 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0127800.1

Sequence Viewer

Length: 381 bp
ATGGTCTTTACCAGGAGATTGGCAAAGCTGATCTGCTACTTCATCAACATGAGCTCGTTTTTCACCGTAGTCAACACTCCGCAAGCCGTCTACTTTGCTCGGAGGGCTACATGCAGCACCAGCCTCTTCGCCTTCGAGTTCTTCAAGGCAGTGGCATTTGCTGTGGTATTCACCGCCTACGGAATCTGCTTCTTCTGGGAGGATTTCCCTGACGCAGAGGTCCGGATCTACATGAAGGTGTCGCTGCTGTCCCTTACCTTTGCCCACCTCTTGCGCTTGTTCTACATTTTACCGGCAAGCAACTTCTGGGTGGTGGTCACCTTCTCTGTCTTCTCTATGTTCATCACTAGGGCTCTGCTCCTCCCCCGCTACCTCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

126

Amino Acids

14.78

Weight (kDa)

9.55

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
AccI GTMKAC 1 cut(s) 90
AccIII TCCGGA 1 cut(s) 222
AciI CCGC 3 cut(s) 80, 174, 367
AclWI GGATC 1 cut(s) 233
AgsI TTSAA 1 cut(s) 145
AjnI CCWGG 1 cut(s) 11
AluBI AGCT 2 cut(s) 28, 54
AluI AGCT 2 cut(s) 28, 54
Alw21I GWGCWC 1 cut(s) 56
AlwI GGATC 1 cut(s) 233
Aor13HI TCCGGA 1 cut(s) 222
ApeKI GCWGC 2 cut(s) 114, 244
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 220
AsuHPI GGTGA 3 cut(s) 55, 163, 310
AvaII GGWCC 1 cut(s) 220
BanII GRGCYC 2 cut(s) 56, 355
BbsI GAAGAC 1 cut(s) 322
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 126, 231
BceAI ACGGC 1 cut(s) 71
BciT130I CCWGG 1 cut(s) 13
BfaI CTAG 2 cut(s) 348, 379
BisI GCNGC 2 cut(s) 115, 245
BlsI GCNGC 2 cut(s) 116, 246
Bme1390I CCNGG 1 cut(s) 13
Bme18I GGWCC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 220
BmrFI CCNGG 1 cut(s) 13
BpiI GAAGAC 1 cut(s) 322
BsaWI WCCGGW 1 cut(s) 222
Bse118I RCCGGY 1 cut(s) 292
BseAI TCCGGA 1 cut(s) 222
BseBI CCWGG 1 cut(s) 13
BseRI GAGGAG 1 cut(s) 350
BseXI GCAGC 2 cut(s) 126, 231
BsiHKAI GWGCWC 1 cut(s) 56
BsiSI CCGG 2 cut(s) 223, 293
BslFI GGGAC 1 cut(s) 235
BsmFI GGGAC 1 cut(s) 235
Bsp1286I GDGCHC 2 cut(s) 56, 355
Bsp13I TCCGGA 1 cut(s) 222
Bsp143I GATC 2 cut(s) 30, 225
BspACI CCGC 3 cut(s) 80, 174, 367
BspEI TCCGGA 1 cut(s) 222
BspPI GGATC 1 cut(s) 233
BsrFI RCCGGY 1 cut(s) 292
BssAI RCCGGY 1 cut(s) 292
BssMI GATC 2 cut(s) 30, 225
Bst2UI CCWGG 1 cut(s) 13
Bst4CI ACNGT 1 cut(s) 67
Bst6I CTCTTC 1 cut(s) 131
BstC8I GCNNGC 2 cut(s) 84, 298
BstEII GGTNACC 1 cut(s) 316
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 2 cut(s) 33, 228
BstMBI GATC 2 cut(s) 30, 225
BstMWI GCNNNNNNNGC 2 cut(s) 104, 120
BstNI CCWGG 1 cut(s) 13
BstNSI RCATGY 1 cut(s) 114
BstPI GGTNACC 1 cut(s) 316
BstSCI CCNGG 1 cut(s) 11
BstV1I GCAGC 2 cut(s) 126, 231
BstV2I GAAGAC 1 cut(s) 322
BstX2I RGATCY 1 cut(s) 225
BstXI CCANNNNNNTGG 1 cut(s) 19
BstYI RGATCY 1 cut(s) 225
BtsI GCAGTG 1 cut(s) 156
BtsIMutI CAGTG 1 cut(s) 156
Cac8I GCNNGC 2 cut(s) 84, 298
CfoI GCGC 1 cut(s) 276
Cfr10I RCCGGY 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 220
CseI GACGC 1 cut(s) 221
CviAII CATG 3 cut(s) 49, 111, 232
CviJI RGCY 6 cut(s) 28, 54, 86, 107, 123, 353
CviKI_1 RGCY 6 cut(s) 28, 54, 86, 107, 123, 353
DpnI GATC 2 cut(s) 32, 227
DpnII GATC 2 cut(s) 30, 225
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Ecl136II GAGCTC 1 cut(s) 54
Eco24I GRGCYC 2 cut(s) 56, 355
Eco47I GGWCC 1 cut(s) 220
Eco53kI GAGCTC 1 cut(s) 54
Eco91I GGTNACC 1 cut(s) 316
EcoICRI GAGCTC 1 cut(s) 54
EcoO65I GGTNACC 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 11
EcoT38I GRGCYC 2 cut(s) 56, 355
FaeI CATG 3 cut(s) 52, 114, 235
FaiI YATR 4 cut(s) 50, 112, 233, 338
FaqI GGGAC 1 cut(s) 235
FatI CATG 3 cut(s) 48, 110, 231
FauI CCCGC 1 cut(s) 374
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 2 cut(s) 115, 245
FriOI GRGCYC 2 cut(s) 56, 355
Fsp4HI GCNGC 2 cut(s) 115, 245
FspBI CTAG 2 cut(s) 348, 379
GlaI GCGC 1 cut(s) 275
GluI GCNGC 2 cut(s) 115, 245
HapII CCGG 2 cut(s) 223, 293
HgaI GACGC 1 cut(s) 221
HhaI GCGC 1 cut(s) 276
Hin1II CATG 3 cut(s) 52, 114, 235
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HinfI GANTC 1 cut(s) 183
HpaII CCGG 2 cut(s) 223, 293
HphI GGTGA 3 cut(s) 55, 163, 310
Hpy166II GTNNAC 2 cut(s) 73, 91
Hpy188I TCNGA 1 cut(s) 102
Hpy188III TCNNGA 1 cut(s) 223
Hpy8I GTNNAC 2 cut(s) 73, 91
HpyAV CCTTC 3 cut(s) 142, 229, 331
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4V TGCA 1 cut(s) 114
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 120
Hsp92II CATG 3 cut(s) 52, 114, 235
HspAI GCGC 1 cut(s) 274
Kpn2I TCCGGA 1 cut(s) 222
Kzo9I GATC 2 cut(s) 30, 225
LmnI GCTCC 1 cut(s) 363
LpnPI CCDG 7 cut(s) 25, 133, 181, 222, 236, 292, 306
Lsp1109I GCAGC 2 cut(s) 126, 231
MaeI CTAG 2 cut(s) 348, 379
MaeIII GTNAC 1 cut(s) 316
MalI GATC 2 cut(s) 32, 227
MboI GATC 2 cut(s) 30, 225
MboII GAAGA 4 cut(s) 118, 133, 184, 322
MflI RGATCY 1 cut(s) 225
MhlI GDGCHC 2 cut(s) 56, 355
MnlI CCTC 6 cut(s) 96, 134, 193, 211, 278, 371
MroI TCCGGA 1 cut(s) 222
MslI CAYNNNNRTG 2 cut(s) 47, 236
MspI CCGG 2 cut(s) 223, 293
MspR9I CCNGG 1 cut(s) 13
MvaI CCWGG 1 cut(s) 13
MwoI GCNNNNNNNGC 2 cut(s) 104, 120
NdeII GATC 2 cut(s) 30, 225
NlaIII CATG 3 cut(s) 52, 114, 235
NmuCI GTSAC 1 cut(s) 316
NspI RCATGY 1 cut(s) 114
PfeI GAWTC 1 cut(s) 183
PkrI GCNGC 2 cut(s) 116, 246
Psp124BI GAGCTC 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 11
PspEI GGTNACC 1 cut(s) 316
PspGI CCWGG 1 cut(s) 11
PspPI GGNCC 1 cut(s) 220
PsuI RGATCY 1 cut(s) 225
RseI CAYNNNNRTG 2 cut(s) 47, 236
SacI GAGCTC 1 cut(s) 56
SatI GCNGC 2 cut(s) 115, 245
Sau3AI GATC 2 cut(s) 30, 225
Sau96I GGNCC 1 cut(s) 220
ScrFI CCNGG 1 cut(s) 13
SduI GDGCHC 2 cut(s) 56, 355
SetI ASST 8 cut(s) 30, 56, 222, 240, 260, 270, 323, 375
SinI GGWCC 1 cut(s) 220
SmiMI CAYNNNNRTG 2 cut(s) 47, 236
SsiI CCGC 3 cut(s) 80, 174, 367
SspMI CTAG 2 cut(s) 348, 379
SstI GAGCTC 1 cut(s) 56
StyD4I CCNGG 1 cut(s) 11
TaaI ACNGT 1 cut(s) 67
TaqI TCGA 1 cut(s) 135
TfiI GAWTC 1 cut(s) 183
TscAI CASTG 1 cut(s) 156
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 2 cut(s) 114, 244
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 3 cut(s) 31, 248, 331
TspGWI ACGGA 1 cut(s) 195
TspRI CASTG 1 cut(s) 156
VpaK11BI GGWCC 1 cut(s) 220
XceI RCATGY 1 cut(s) 114
XmiI GTMKAC 1 cut(s) 90
XspI CTAG 2 cut(s) 348, 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.