Rorug02G0518900

hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005254
Physical Location & Seq
Forward (+)
65011192 .. 65012413
1222 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0518900.1

Sequence Viewer

Length: 684 bp
ATGAGGTTGACGCTGATGTCTGAAGAAGCCTGGTTCAGTGGCGCTGGGAAGGAGATTTGGGAATTCTCTCCATGGTGGAATTGTCCTTGGCTTGGATGGCAAAATATGGGGGTCGAGTTTCACACCCTCAATCCCAACTTTACGGTGGCGGTTTCTGGGAAGCTAGAGTCAATTTTGTGGAGAATGAAAGTTGATTGTGGCAGCTCATCTTATTTTTTTAGGGTTTTACTTTTTAGTTCGTGGTTAAGCTTAATAAGTCCCTCCTCTTTTGAGCGAGGGTTTGGGTGTTTTAGTGTAGGGTCGATGCCATTGTTATGGTGTCATGTTCGTACTGTTGTGCCATCTCTATGGCGTTATTACCGAGGACTATCCGTTCTATTGTCGGTGCTTTCTGGTTGTCAACGTGATGGTGAATCGCCCTTACACGTGGTCTGGCTGTTTTGGGACACGGTGTCGGAAATGGTGAAGTGGTTCGTCTTAATGTTAGTGGCGGGGTTGTATCGTTACACTCGGTATTGGTTACTATTCATTGTAGCCCGCTGGTATGAGCGTAAAATTTGGTTAGGGGTTGGGCCCTTTTGGCTCATGGATGTCATTCATATTGACGGACCTATAACTATGGATCAGGTTAGGAATGTGTGTCGTGGTGTTGTTACCACAACCGGTCGTTCCAATGCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

227

Amino Acids

26.45

Weight (kDa)

8.8

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 16
AciI CCGC 3 cut(s) 149, 491, 538
AclWI GGATC 1 cut(s) 630
AcsI RAATTY 2 cut(s) 62, 555
AcuI CTGAAG 1 cut(s) 42
AcvI CACGTG 1 cut(s) 427
AfaI GTAC 1 cut(s) 331
AfiI CCNNNNNNNGG 1 cut(s) 92
AflIII ACRYGT 1 cut(s) 424
AgeI ACCGGT 1 cut(s) 662
AhdI GACNNNNNGTC 1 cut(s) 451
AjnI CCWGG 1 cut(s) 29
AloI GAACNNNNNNTCC 2 cut(s) 357, 389
AluBI AGCT 3 cut(s) 163, 204, 249
AluI AGCT 3 cut(s) 163, 204, 249
AlwI GGATC 1 cut(s) 630
AoxI GGCC 1 cut(s) 572
ApaI GGGCCC 1 cut(s) 576
ApeKI GCWGC 1 cut(s) 201
ApoI RAATTY 2 cut(s) 62, 555
AsiGI ACCGGT 1 cut(s) 662
Asp700I GAANNNNTTC 1 cut(s) 470
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 3 cut(s) 572, 573, 608
AsuHPI GGTGA 2 cut(s) 422, 475
AvaII GGWCC 1 cut(s) 608
BaeGI GKGCMC 1 cut(s) 576
BanII GRGCYC 1 cut(s) 576
BbrPI CACGTG 1 cut(s) 427
BbvI GCAGC 1 cut(s) 213
BccI CCATC 3 cut(s) 90, 349, 401
BciT130I CCWGG 1 cut(s) 31
BfaI CTAG 1 cut(s) 164
BfoI RGCGCY 1 cut(s) 45
BglI GCCNNNNNGGC 1 cut(s) 580
BisI GCNGC 1 cut(s) 202
BlsI GCNGC 1 cut(s) 203
Bme1390I CCNGG 1 cut(s) 31
Bme18I GGWCC 1 cut(s) 608
BmeRI GACNNNNNGTC 1 cut(s) 451
BmgT120I GGNCC 3 cut(s) 572, 573, 608
BmiI GGNNCC 1 cut(s) 574
BmrFI CCNGG 1 cut(s) 31
BmsI GCATC 1 cut(s) 294
BsaAI YACGTR 1 cut(s) 427
BsaJI CCNNGG 3 cut(s) 71, 86, 361
BsaWI WCCGGW 1 cut(s) 662
Bsc4I CCNNNNNNNGG 1 cut(s) 92
Bse118I RCCGGY 1 cut(s) 662
BseBI CCWGG 1 cut(s) 31
BseDI CCNNGG 3 cut(s) 71, 86, 361
BseGI GGATG 2 cut(s) 101, 595
BseLI CCNNNNNNNGG 1 cut(s) 92
BseRI GAGGAG 1 cut(s) 253
BseSI GKGCMC 1 cut(s) 576
BseXI GCAGC 1 cut(s) 213
BseYI CCCAGC 1 cut(s) 44
Bsh1285I CGRYCG 1 cut(s) 667
BshFI GGCC 1 cut(s) 574
BshTI ACCGGT 1 cut(s) 662
BsiEI CGRYCG 1 cut(s) 667
BsiSI CCGG 1 cut(s) 663
BslFI GGGAC 2 cut(s) 243, 458
BslI CCNNNNNNNGG 1 cut(s) 92
BsmFI GGGAC 2 cut(s) 243, 458
BsnI GGCC 1 cut(s) 574
Bsp120I GGGCCC 1 cut(s) 572
Bsp1286I GDGCHC 1 cut(s) 576
Bsp143I GATC 1 cut(s) 622
Bsp19I CCATGG 1 cut(s) 71
BspACI CCGC 3 cut(s) 149, 491, 538
BspANI GGCC 1 cut(s) 574
BspLI GGNNCC 1 cut(s) 574
BspPI GGATC 1 cut(s) 630
BsrFI RCCGGY 1 cut(s) 662
BssAI RCCGGY 1 cut(s) 662
BssECI CCNNGG 3 cut(s) 71, 86, 361
BssMI GATC 1 cut(s) 622
BssT1I CCWWGG 2 cut(s) 71, 86
Bst2UI CCWGG 1 cut(s) 31
Bst4CI ACNGT 3 cut(s) 145, 334, 451
BstBAI YACGTR 1 cut(s) 427
BstC8I GCNNGC 1 cut(s) 538
BstDSI CCRYGG 1 cut(s) 71
BstF5I GGATG 2 cut(s) 101, 595
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 1 cut(s) 625
BstMBI GATC 1 cut(s) 622
BstMCI CGRYCG 1 cut(s) 667
BstMWI GCNNNNNNNGC 2 cut(s) 97, 580
BstNI CCWGG 1 cut(s) 31
BstSCI CCNGG 1 cut(s) 29
BstSLI GKGCMC 1 cut(s) 576
BstV1I GCAGC 1 cut(s) 213
BstXI CCANNNNNNTGG 2 cut(s) 315, 348
BsuRI GGCC 1 cut(s) 574
BtgI CCRYGG 1 cut(s) 71
BtsCI GGATG 2 cut(s) 101, 595
BtsIMutI CAGTG 1 cut(s) 43
Cac8I GCNNGC 1 cut(s) 538
CfoI GCGC 1 cut(s) 44
Cfr10I RCCGGY 1 cut(s) 662
Cfr13I GGNCC 3 cut(s) 572, 573, 608
CseI GACGC 1 cut(s) 19
Csp6I GTAC 1 cut(s) 330
CspAI ACCGGT 1 cut(s) 662
CviAII CATG 3 cut(s) 72, 323, 586
CviJI RGCY 9 cut(s) 29, 91, 163, 204, 249, 436, 536, 574, 583
CviKI_1 RGCY 9 cut(s) 29, 91, 163, 204, 249, 436, 536, 574, 583
CviQI GTAC 1 cut(s) 330
DpnI GATC 1 cut(s) 624
DpnII GATC 1 cut(s) 622
DrdI GACNNNNNNGTC 1 cut(s) 16
DriI GACNNNNNGTC 1 cut(s) 451
DseDI GACNNNNNNGTC 1 cut(s) 16
Eam1105I GACNNNNNGTC 1 cut(s) 451
Eco130I CCWWGG 2 cut(s) 71, 86
Eco24I GRGCYC 1 cut(s) 576
Eco47I GGWCC 1 cut(s) 608
Eco57I CTGAAG 1 cut(s) 42
Eco72I CACGTG 1 cut(s) 427
EcoO109I RGGNCCY 1 cut(s) 573
EcoRI GAATTC 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 29
EcoT14I CCWWGG 2 cut(s) 71, 86
EcoT38I GRGCYC 1 cut(s) 576
ErhI CCWWGG 2 cut(s) 71, 86
FaeI CATG 3 cut(s) 75, 326, 589
FaqI GGGAC 2 cut(s) 243, 458
FatI CATG 3 cut(s) 71, 322, 585
FauI CCCGC 2 cut(s) 484, 545
Fnu4HI GCNGC 1 cut(s) 202
FokI GGATG 2 cut(s) 108, 602
FriOI GRGCYC 1 cut(s) 576
Fsp4HI GCNGC 1 cut(s) 202
FspBI CTAG 1 cut(s) 164
GlaI GCGC 1 cut(s) 43
GluI GCNGC 1 cut(s) 202
GsaI CCCAGC 1 cut(s) 48
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 1 cut(s) 574
HapII CCGG 1 cut(s) 663
HgaI GACGC 1 cut(s) 19
HhaI GCGC 1 cut(s) 44
Hin1II CATG 3 cut(s) 75, 326, 589
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HincII GTYRAC 2 cut(s) 9, 401
HindII GTYRAC 2 cut(s) 9, 401
HindIII AAGCTT 1 cut(s) 247
HinfI GANTC 2 cut(s) 167, 413
HpaII CCGG 1 cut(s) 663
HphI GGTGA 2 cut(s) 422, 475
Hpy166II GTNNAC 2 cut(s) 9, 401
Hpy188I TCNGA 2 cut(s) 22, 457
Hpy8I GTNNAC 2 cut(s) 9, 401
HpyAV CCTTC 1 cut(s) 43
HpyCH4III ACNGT 3 cut(s) 145, 334, 451
HpyCH4IV ACGT 2 cut(s) 403, 426
HpyF10VI GCNNNNNNNGC 2 cut(s) 97, 580
HpySE526I ACGT 2 cut(s) 403, 426
Hsp92II CATG 3 cut(s) 75, 326, 589
HspAI GCGC 1 cut(s) 42
Kzo9I GATC 1 cut(s) 622
LpnPI CCDG 9 cut(s) 16, 30, 43, 141, 378, 418, 526, 611, 676
Lsp1109I GCAGC 1 cut(s) 213
LweI GCATC 1 cut(s) 294
MaeI CTAG 1 cut(s) 164
MaeII ACGT 2 cut(s) 403, 426
MaeIII GTNAC 3 cut(s) 503, 519, 652
MalI GATC 1 cut(s) 624
MboI GATC 1 cut(s) 622
MboII GAAGA 1 cut(s) 35
MhlI GDGCHC 1 cut(s) 576
MluCI AATT 4 cut(s) 62, 79, 171, 555
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 435
MnlI CCTC 5 cut(s) 137, 269, 271, 274, 356
MroXI GAANNNNTTC 1 cut(s) 470
MseI TTAA 3 cut(s) 245, 251, 479
MslI CAYNNNNRTG 2 cut(s) 313, 346
MspA1I CMGCKG 1 cut(s) 540
MspI CCGG 1 cut(s) 663
MspR9I CCNGG 1 cut(s) 31
MvaI CCWGG 1 cut(s) 31
MwoI GCNNNNNNNGC 2 cut(s) 97, 580
NcoI CCATGG 1 cut(s) 71
NdeII GATC 1 cut(s) 622
NlaIII CATG 3 cut(s) 75, 326, 589
NlaIV GGNNCC 1 cut(s) 574
PdmI GAANNNNTTC 1 cut(s) 470
PfeI GAWTC 1 cut(s) 413
PinAI ACCGGT 1 cut(s) 662
PkrI GCNGC 1 cut(s) 203
PleI GAGTC 1 cut(s) 175
PmaCI CACGTG 1 cut(s) 427
PmlI CACGTG 1 cut(s) 427
PpsI GAGTC 1 cut(s) 175
Ppu21I YACGTR 1 cut(s) 427
Psp6I CCWGG 1 cut(s) 29
PspCI CACGTG 1 cut(s) 427
PspFI CCCAGC 1 cut(s) 44
PspGI CCWGG 1 cut(s) 29
PspN4I GGNNCC 1 cut(s) 574
PspOMI GGGCCC 1 cut(s) 572
PspPI GGNCC 3 cut(s) 572, 573, 608
RsaI GTAC 1 cut(s) 331
RsaNI GTAC 1 cut(s) 330
RseI CAYNNNNRTG 2 cut(s) 313, 346
SaqAI TTAA 3 cut(s) 245, 251, 479
SatI GCNGC 1 cut(s) 202
Sau3AI GATC 1 cut(s) 622
Sau96I GGNCC 3 cut(s) 572, 573, 608
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 1 cut(s) 31
SduI GDGCHC 1 cut(s) 576
SetI ASST 8 cut(s) 8, 165, 206, 251, 406, 429, 613, 630
SfaNI GCATC 1 cut(s) 294
SinI GGWCC 1 cut(s) 608
SmiMI CAYNNNNRTG 2 cut(s) 313, 346
Sse9I AATT 4 cut(s) 62, 79, 171, 555
SsiI CCGC 3 cut(s) 149, 491, 538
SspMI CTAG 1 cut(s) 164
StyD4I CCNGG 1 cut(s) 29
StyI CCWWGG 2 cut(s) 71, 86
TaaI ACNGT 3 cut(s) 145, 334, 451
TaiI ACGT 2 cut(s) 406, 429
TaqI TCGA 2 cut(s) 114, 302
TasI AATT 4 cut(s) 62, 79, 171, 555
TfiI GAWTC 1 cut(s) 413
Tru1I TTAA 3 cut(s) 245, 251, 479
Tru9I TTAA 3 cut(s) 245, 251, 479
TscAI CASTG 1 cut(s) 43
TseI GCWGC 1 cut(s) 201
TspDTI ATGAA 3 cut(s) 200, 517, 587
TspGWI ACGGA 2 cut(s) 361, 621
TspRI CASTG 1 cut(s) 43
VpaK11BI GGWCC 1 cut(s) 608
XapI RAATTY 2 cut(s) 62, 555
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
XmnI GAANNNNTTC 1 cut(s) 470
XspI CTAG 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.