Rh2AG584600

hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0005254
Physical Location & Seq
Forward (+)
81778780 .. 81782580
3801 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG584600.1

Sequence Viewer

Length: 816 bp
ATGATACCTCTGGTGTTTGGTTCTTCACTGCCCTCACCAACACCGCCAGAGGCAGTAGAGACGCCGGCTACAAAGAAATGTGGGAGCTTAACGGGTCCCCGGATCTTCCCAAATGGAAGCCGTTCGGGTTCCGGTAACGGGTTCCCGCCGTTTCTTCCCAAAGAGGTTGAGAAGATCAGAGACCCTTATGCCAGAAACTTGGCTCAAAGAATGGAGAGGCTCCCTGTGCAGATGGGGTTATATGAAAGTTGTATAATGAGTAGTTGTGTGAAACCTCTAGTACAAACCAAGAACAATCCGGTGGTTCTTCTCCATTGCTTTGACAGTTCTTGTTTAGAATGGAGGTGCACATATCCCCTGCTTGAAGAGGCCGGGTTGGAGGCTTGGGCGATTGATGTGCTCGGGTGGGGCTTCTCCGATTTAGAAAGGCTTCCACCATGCAATCCGGAATCAAAGCGTCATCACCTCTATCAGTTTTGGAAGTCCTACATCAAAAGGCCGATGATATTAGTTGGACCAAGCCTCGGTGCTTCTGTTGCAATTGACTTTGCAGTTAGTTATCCGTTTGCTGTTGAAAAGCTGGTTTTGATTAATGCAAGTGTGTATGCAGAAGGCACCAAAGGCCTTGCAGAATTTCCTAGAGTATTAGCCTATGCTGGGGTTTCCTTGTTGAGGAGCATCCCTCTACGGCTTTACGCCAATACGCTGGCCTTCAATGACATTTCATTAGCTACAACCTTTGATTGGACTAATGTACAATTATCTTTTGTTAACCAATATTTGTTCAACTTTACATTGACGTGTTTGTTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

271

Amino Acids

30.18

Weight (kDa)

8.08

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 99 - 199 3.4e-06 alpha/beta hydrolase fold
Hydrolase_4 PF12146 101 - 215 8.5e-07 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 101 - 220 1.3e-06 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 614
AccIII TCCGGA 1 cut(s) 445
AciI CCGC 2 cut(s) 44, 146
AclWI GGATC 1 cut(s) 110
AcsI RAATTY 1 cut(s) 632
AcyI GRCGYC 1 cut(s) 62
AfaI GTAC 2 cut(s) 282, 756
AfiI CCNNNNNNNGG 5 cut(s) 138, 524, 657, 672, 744
AflIII ACRYGT 1 cut(s) 800
AgsI TTSAA 4 cut(s) 365, 575, 715, 787
AjiI CACGTC 1 cut(s) 801
AluBI AGCT 3 cut(s) 87, 580, 731
AluI AGCT 3 cut(s) 87, 580, 731
Alw21I GWGCWC 2 cut(s) 350, 402
Alw26I GTCTC 2 cut(s) 53, 174
Alw44I GTGCAC 1 cut(s) 346
AlwI GGATC 1 cut(s) 110
Ama87I CYCGRG 1 cut(s) 401
Aor13HI TCCGGA 1 cut(s) 445
AoxI GGCC 4 cut(s) 369, 497, 622, 708
ApaLI GTGCAC 1 cut(s) 346
ApoI RAATTY 1 cut(s) 632
AseI ATTAAT 1 cut(s) 591
Asp700I GAANNNNTTC 2 cut(s) 121, 429
AspS9I GGNCC 2 cut(s) 95, 515
AsuC2I CCSGG 2 cut(s) 100, 373
AsuHPI GGTGA 2 cut(s) 27, 455
AvaI CYCGRG 1 cut(s) 401
AvaII GGWCC 2 cut(s) 95, 515
BaeGI GKGCMC 1 cut(s) 350
BanI GGYRCC 1 cut(s) 614
Bbv12I GWGCWC 2 cut(s) 350, 402
BccI CCATC 1 cut(s) 226
BceAI ACGGC 3 cut(s) 105, 133, 704
BcgI CGANNNNNNTGC 2 cut(s) 379, 413
BcnI CCSGG 2 cut(s) 100, 373
BcoDI GTCTC 2 cut(s) 53, 174
BfaI CTAG 2 cut(s) 278, 639
Bme1390I CCNGG 2 cut(s) 100, 373
Bme18I GGWCC 2 cut(s) 95, 515
BmeT110I CYCGRG 1 cut(s) 401
BmgBI CACGTC 1 cut(s) 801
BmgT120I GGNCC 2 cut(s) 95, 515
BmiI GGNNCC 6 cut(s) 96, 97, 130, 143, 221, 616
BmrFI CCNGG 2 cut(s) 100, 373
BmsI GCATC 1 cut(s) 687
BplI GAGNNNNNCTC 2 cut(s) 667, 699
BpuMI CCSGG 2 cut(s) 100, 373
BsaHI GRCGYC 1 cut(s) 62
BsaI GGTCTC 1 cut(s) 174
BsaJI CCNNGG 2 cut(s) 98, 523
BsaWI WCCGGW 3 cut(s) 131, 298, 445
Bsc4I CCNNNNNNNGG 5 cut(s) 138, 524, 657, 672, 744
Bse118I RCCGGY 1 cut(s) 64
Bse3DI GCAATG 1 cut(s) 313
BseAI TCCGGA 1 cut(s) 445
BseDI CCNNGG 2 cut(s) 98, 523
BseGI GGATG 1 cut(s) 678
BseLI CCNNNNNNNGG 5 cut(s) 138, 524, 657, 672, 744
BseMI GCAATG 1 cut(s) 313
BseRI GAGGAG 1 cut(s) 688
BseSI GKGCMC 1 cut(s) 350
BseYI CCCAGC 1 cut(s) 656
BsgI GTGCAG 1 cut(s) 248
BshFI GGCC 4 cut(s) 371, 499, 624, 710
BshNI GGYRCC 1 cut(s) 614
BsiHKAI GWGCWC 2 cut(s) 350, 402
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 6 cut(s) 65, 100, 132, 299, 372, 446
BslFI GGGAC 1 cut(s) 81
BslI CCNNNNNNNGG 5 cut(s) 138, 524, 657, 672, 744
BsmAI GTCTC 2 cut(s) 53, 174
BsmBI CGTCTC 1 cut(s) 53
BsmFI GGGAC 1 cut(s) 81
BsnI GGCC 4 cut(s) 371, 499, 624, 710
Bso31I GGTCTC 1 cut(s) 174
BsoBI CYCGRG 1 cut(s) 401
Bsp1286I GDGCHC 2 cut(s) 350, 402
Bsp13I TCCGGA 1 cut(s) 445
Bsp1407I TGTACA 1 cut(s) 754
Bsp143I GATC 2 cut(s) 102, 174
BspACI CCGC 2 cut(s) 44, 146
BspANI GGCC 4 cut(s) 371, 499, 624, 710
BspEI TCCGGA 1 cut(s) 445
BspLI GGNNCC 6 cut(s) 96, 97, 130, 143, 221, 616
BspPI GGATC 1 cut(s) 110
BspT107I GGYRCC 1 cut(s) 614
BspTNI GGTCTC 1 cut(s) 174
BsrDI GCAATG 1 cut(s) 313
BsrFI RCCGGY 1 cut(s) 64
BsrGI TGTACA 1 cut(s) 754
BssAI RCCGGY 1 cut(s) 64
BssECI CCNNGG 2 cut(s) 98, 523
BssMI GATC 2 cut(s) 102, 174
BssNI GRCGYC 1 cut(s) 62
Bst4CI ACNGT 1 cut(s) 326
Bst6I CTCTTC 1 cut(s) 360
BstACI GRCGYC 1 cut(s) 62
BstAUI TGTACA 1 cut(s) 754
BstC8I GCNNGC 2 cut(s) 66, 708
BstENI CCTNNNNNAGG 1 cut(s) 670
BstF5I GGATG 1 cut(s) 678
BstKTI GATC 2 cut(s) 105, 177
BstMAI GTCTC 2 cut(s) 53, 174
BstMBI GATC 2 cut(s) 102, 174
BstMWI GCNNNNNNNGC 3 cut(s) 226, 536, 621
BstSCI CCNGG 2 cut(s) 98, 371
BstSLI GKGCMC 1 cut(s) 350
BstX2I RGATCY 1 cut(s) 102
BstXI CCANNNNNNTGG 2 cut(s) 199, 706
BstYI RGATCY 1 cut(s) 102
BsuRI GGCC 4 cut(s) 371, 499, 624, 710
BtrI CACGTC 1 cut(s) 801
BtsCI GGATG 1 cut(s) 678
BtsI GCAGTG 1 cut(s) 26
BtsIMutI CAGTG 1 cut(s) 26
Cac8I GCNNGC 2 cut(s) 66, 708
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 2 cut(s) 95, 515
CseI GACGC 2 cut(s) 70, 446
Csp6I GTAC 2 cut(s) 281, 755
CviAII CATG 1 cut(s) 438
CviQI GTAC 2 cut(s) 281, 755
DpnI GATC 2 cut(s) 104, 176
DpnII GATC 2 cut(s) 102, 174
Eam1104I CTCTTC 1 cut(s) 360
EarI CTCTTC 1 cut(s) 360
Eco147I AGGCCT 1 cut(s) 624
Eco31I GGTCTC 1 cut(s) 174
Eco47I GGWCC 2 cut(s) 95, 515
Eco88I CYCGRG 1 cut(s) 401
EcoNI CCTNNNNNAGG 1 cut(s) 670
EcoO109I RGGNCCY 1 cut(s) 95
Esp3I CGTCTC 1 cut(s) 53
FaeI CATG 1 cut(s) 441
FaiI YATR 8 cut(s) 189, 241, 243, 254, 352, 439, 606, 654
FaqI GGGAC 1 cut(s) 81
FatI CATG 1 cut(s) 437
FauI CCCGC 1 cut(s) 153
FokI GGATG 1 cut(s) 665
FspBI CTAG 2 cut(s) 278, 639
GsaI CCCAGC 1 cut(s) 660
HaeIII GGCC 4 cut(s) 371, 499, 624, 710
HapII CCGG 6 cut(s) 65, 100, 132, 299, 372, 446
HgaI GACGC 2 cut(s) 70, 446
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 1 cut(s) 441
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HinfI GANTC 1 cut(s) 449
HpaI GTTAAC 1 cut(s) 772
HpaII CCGG 6 cut(s) 65, 100, 132, 299, 372, 446
HphI GGTGA 2 cut(s) 27, 455
Hpy166II GTNNAC 2 cut(s) 348, 772
Hpy188I TCNGA 2 cut(s) 179, 418
Hpy188III TCNNGA 1 cut(s) 446
Hpy8I GTNNAC 2 cut(s) 348, 772
HpyAV CCTTC 2 cut(s) 605, 721
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4IV ACGT 1 cut(s) 800
HpyCH4V TGCA 8 cut(s) 229, 348, 441, 539, 551, 596, 608, 629
HpyF10VI GCNNNNNNNGC 3 cut(s) 226, 536, 621
HpySE526I ACGT 1 cut(s) 800
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 1 cut(s) 441
KflI GGGWCCC 1 cut(s) 95
Kpn2I TCCGGA 1 cut(s) 445
KroI GCCGGC 1 cut(s) 64
KroNI GCCGGC 1 cut(s) 66
KspAI GTTAAC 1 cut(s) 772
Kzo9I GATC 2 cut(s) 102, 174
LmnI GCTCC 3 cut(s) 84, 225, 675
LweI GCATC 1 cut(s) 687
MaeI CTAG 2 cut(s) 278, 639
MaeII ACGT 1 cut(s) 800
MaeIII GTNAC 1 cut(s) 134
MalI GATC 2 cut(s) 104, 176
MboI GATC 2 cut(s) 102, 174
MboII GAAGA 6 cut(s) 15, 97, 146, 184, 299, 377
MfeI CAATTG 1 cut(s) 540
MflI RGATCY 1 cut(s) 102
MhlI GDGCHC 2 cut(s) 350, 402
MluCI AATT 3 cut(s) 540, 632, 758
MmeI TCCRAC 2 cut(s) 357, 493
MroI TCCGGA 1 cut(s) 445
MroNI GCCGGC 1 cut(s) 64
MroXI GAANNNNTTC 2 cut(s) 121, 429
MseI TTAA 4 cut(s) 89, 591, 771, 814
MslI CAYNNNNRTG 1 cut(s) 799
MspI CCGG 6 cut(s) 65, 100, 132, 299, 372, 446
MspR9I CCNGG 2 cut(s) 100, 373
MunI CAATTG 1 cut(s) 540
MwoI GCNNNNNNNGC 3 cut(s) 226, 536, 621
NaeI GCCGGC 1 cut(s) 66
NciI CCSGG 2 cut(s) 100, 373
NdeII GATC 2 cut(s) 102, 174
NgoMIV GCCGGC 1 cut(s) 64
NlaIII CATG 1 cut(s) 441
NlaIV GGNNCC 6 cut(s) 96, 97, 130, 143, 221, 616
PceI AGGCCT 1 cut(s) 624
PdiI GCCGGC 1 cut(s) 66
PdmI GAANNNNTTC 2 cut(s) 121, 429
PfeI GAWTC 1 cut(s) 449
PpuMI RGGWCCY 1 cut(s) 95
PshBI ATTAAT 1 cut(s) 591
Psp5II RGGWCCY 1 cut(s) 95
PspFI CCCAGC 1 cut(s) 656
PspN4I GGNNCC 6 cut(s) 96, 97, 130, 143, 221, 616
PspPI GGNCC 2 cut(s) 95, 515
PspPPI RGGWCCY 1 cut(s) 95
PsuI RGATCY 1 cut(s) 102
RsaI GTAC 2 cut(s) 282, 756
RsaNI GTAC 2 cut(s) 281, 755
RseI CAYNNNNRTG 1 cut(s) 799
SaqAI TTAA 4 cut(s) 89, 591, 771, 814
Sau3AI GATC 2 cut(s) 102, 174
Sau96I GGNCC 2 cut(s) 95, 515
ScrFI CCNGG 2 cut(s) 100, 373
SduI GDGCHC 2 cut(s) 350, 402
SfaNI GCATC 1 cut(s) 687
SinI GGWCC 2 cut(s) 95, 515
SmiMI CAYNNNNRTG 1 cut(s) 799
Sse9I AATT 3 cut(s) 540, 632, 758
SseBI AGGCCT 1 cut(s) 624
SsiI CCGC 2 cut(s) 44, 146
SspI AATATT 1 cut(s) 779
SspMI CTAG 2 cut(s) 278, 639
StuI AGGCCT 1 cut(s) 624
StyD4I CCNGG 2 cut(s) 98, 371
TaaI ACNGT 1 cut(s) 326
TaiI ACGT 1 cut(s) 803
TasI AATT 3 cut(s) 540, 632, 758
TatI WGTACW 2 cut(s) 280, 754
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 4 cut(s) 89, 591, 771, 814
Tru9I TTAA 4 cut(s) 89, 591, 771, 814
TscAI CASTG 1 cut(s) 33
TspDTI ATGAA 2 cut(s) 258, 714
TspGWI ACGGA 1 cut(s) 552
TspRI CASTG 1 cut(s) 33
VneI GTGCAC 1 cut(s) 346
VpaK11BI GGWCC 2 cut(s) 95, 515
VspI ATTAAT 1 cut(s) 591
XagI CCTNNNNNAGG 1 cut(s) 670
XapI RAATTY 1 cut(s) 632
XmnI GAANNNNTTC 2 cut(s) 121, 429
XspI CTAG 2 cut(s) 278, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.