Rw2G048740

hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005254
Physical Location & Seq
Forward (+)
76305839 .. 76309624
3786 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G048740.1

Sequence Viewer

Length: 633 bp
ATGGAGAGGCTCCCTGTGCAGATGGGGTTATATGAAAGTTGTATAATGAGTAGTTGTGTGAAACCTCTAGTACAAACCAAGAACAATCCAGTGGTTCTTCTCCATTGCTTTGACAGTTCTTGTTTAGAATGGAGGTGCACATATCCCCTGCTTGAAGAGGCCGGGTTGGAGGCTTGGGCGATTGATGTGCTCGGGTGGGGCTTCTCCGATTTAGAAAGGCTTCCACCATGCAATCAGGAATCAAAGCGTCATCACCTCTATCAGTTTTGGAAGTCCTACATCAGAAGGCCGATGATATTAGTTGGACCAAGCCTCGGTGCTTCTGTTGCAATTGACTTTGCAGTTAGTTATCCGTTTGCTGTTGAAAAGCTGGTTTTGATTAATGCAAGTGTGTACGCAGAAGGCACCAAAGGCCTTGCAGAATTTCCTAGAGTATTAGCCTATGCTGGGGTTTCCTTGTTGAGCAGCATCCCTCTACGGCTTTACGCCAATACGCTGGCCTTCAATGACATTTCATTAGCTACAACCTTTGATTGGACTAATGTCGGCCGCTTACACTGTTTGCTGCCTTGGTGGAAAGATGCAACAGTAAGTTTCATGAGTAGTGGGGGCTACAATGTTATTGCCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

210

Amino Acids

23.52

Weight (kDa)

6.41

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 29 - 129 7.2e-07 alpha/beta hydrolase fold
Hydrolase_4 PF12146 31 - 141 3.6e-07 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 31 - 156 7.1e-07 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 404
AciI CCGC 1 cut(s) 550
AcoI YGGCCR 1 cut(s) 547
AcsI RAATTY 1 cut(s) 422
AfaI GTAC 2 cut(s) 72, 395
AfiI CCNNNNNNNGG 3 cut(s) 314, 447, 534
AgsI TTSAA 3 cut(s) 155, 365, 505
AluBI AGCT 2 cut(s) 370, 521
AluI AGCT 2 cut(s) 370, 521
Alw21I GWGCWC 2 cut(s) 140, 192
Alw44I GTGCAC 1 cut(s) 136
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 5 cut(s) 159, 287, 412, 498, 547
ApaLI GTGCAC 1 cut(s) 136
ApeKI GCWGC 2 cut(s) 465, 565
ApoI RAATTY 1 cut(s) 422
AseI ATTAAT 1 cut(s) 381
Asp700I GAANNNNTTC 1 cut(s) 219
AspS9I GGNCC 1 cut(s) 305
AsuC2I CCSGG 1 cut(s) 163
AsuHPI GGTGA 1 cut(s) 245
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 1 cut(s) 305
BaeGI GKGCMC 1 cut(s) 140
BanI GGYRCC 1 cut(s) 404
Bbv12I GWGCWC 2 cut(s) 140, 192
BbvI GCAGC 2 cut(s) 477, 552
BccI CCATC 1 cut(s) 16
BceAI ACGGC 1 cut(s) 494
BcgI CGANNNNNNTGC 2 cut(s) 169, 203
BcnI CCSGG 1 cut(s) 163
BfaI CTAG 2 cut(s) 68, 429
BisI GCNGC 3 cut(s) 466, 550, 566
BlsI GCNGC 3 cut(s) 467, 551, 567
Bme1390I CCNGG 1 cut(s) 163
Bme18I GGWCC 1 cut(s) 305
BmeT110I CYCGRG 1 cut(s) 191
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 2 cut(s) 11, 406
BmrFI CCNGG 1 cut(s) 163
BmsI GCATC 2 cut(s) 477, 571
BoxI GACNNNNGTC 1 cut(s) 542
BpuMI CCSGG 1 cut(s) 163
BsaJI CCNNGG 2 cut(s) 313, 569
Bsc4I CCNNNNNNNGG 3 cut(s) 314, 447, 534
Bse1I ACTGG 1 cut(s) 89
Bse3DI GCAATG 1 cut(s) 103
BseDI CCNNGG 2 cut(s) 313, 569
BseGI GGATG 1 cut(s) 468
BseLI CCNNNNNNNGG 3 cut(s) 314, 447, 534
BseMI GCAATG 1 cut(s) 103
BseNI ACTGG 1 cut(s) 89
BseSI GKGCMC 1 cut(s) 140
BseX3I CGGCCG 1 cut(s) 547
BseXI GCAGC 2 cut(s) 477, 552
BseYI CCCAGC 1 cut(s) 446
BsgI GTGCAG 1 cut(s) 38
Bsh1285I CGRYCG 1 cut(s) 550
BshFI GGCC 5 cut(s) 161, 289, 414, 500, 549
BshNI GGYRCC 1 cut(s) 404
BsiEI CGRYCG 1 cut(s) 550
BsiHKAI GWGCWC 2 cut(s) 140, 192
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 1 cut(s) 162
BslI CCNNNNNNNGG 3 cut(s) 314, 447, 534
BsnI GGCC 5 cut(s) 161, 289, 414, 500, 549
BsoBI CYCGRG 1 cut(s) 191
Bsp1286I GDGCHC 2 cut(s) 140, 192
BspACI CCGC 1 cut(s) 550
BspANI GGCC 5 cut(s) 161, 289, 414, 500, 549
BspHI TCATGA 1 cut(s) 597
BspLI GGNNCC 2 cut(s) 11, 406
BspT107I GGYRCC 1 cut(s) 404
BsrDI GCAATG 1 cut(s) 103
BsrI ACTGG 1 cut(s) 89
BssECI CCNNGG 2 cut(s) 313, 569
BssT1I CCWWGG 1 cut(s) 569
Bst4CI ACNGT 3 cut(s) 116, 560, 589
Bst6I CTCTTC 1 cut(s) 150
BstC8I GCNNGC 1 cut(s) 498
BstF5I GGATG 1 cut(s) 468
BstMCI CGRYCG 1 cut(s) 550
BstMWI GCNNNNNNNGC 3 cut(s) 16, 326, 411
BstPAI GACNNNNGTC 1 cut(s) 542
BstSCI CCNGG 1 cut(s) 161
BstSLI GKGCMC 1 cut(s) 140
BstV1I GCAGC 2 cut(s) 477, 552
BstXI CCANNNNNNTGG 1 cut(s) 496
BstZI CGGCCG 1 cut(s) 547
BsuRI GGCC 5 cut(s) 161, 289, 414, 500, 549
BtsCI GGATG 1 cut(s) 468
BtsIMutI CAGTG 2 cut(s) 96, 556
Cac8I GCNNGC 1 cut(s) 498
CciI TCATGA 1 cut(s) 597
Cfr13I GGNCC 1 cut(s) 305
CseI GACGC 1 cut(s) 236
Csp6I GTAC 2 cut(s) 71, 394
CviAII CATG 2 cut(s) 228, 598
CviQI GTAC 2 cut(s) 71, 394
EaeI YGGCCR 1 cut(s) 547
EagI CGGCCG 1 cut(s) 547
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
EclXI CGGCCG 1 cut(s) 547
Eco130I CCWWGG 1 cut(s) 569
Eco147I AGGCCT 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 305
Eco52I CGGCCG 1 cut(s) 547
Eco88I CYCGRG 1 cut(s) 191
EcoT14I CCWWGG 1 cut(s) 569
ErhI CCWWGG 1 cut(s) 569
FaeI CATG 2 cut(s) 231, 601
FaiI YATR 7 cut(s) 31, 33, 44, 142, 229, 444, 599
FatI CATG 2 cut(s) 227, 597
Fnu4HI GCNGC 3 cut(s) 466, 550, 566
FokI GGATG 1 cut(s) 455
Fsp4HI GCNGC 3 cut(s) 466, 550, 566
FspBI CTAG 2 cut(s) 68, 429
GluI GCNGC 3 cut(s) 466, 550, 566
GsaI CCCAGC 1 cut(s) 450
HaeIII GGCC 5 cut(s) 161, 289, 414, 500, 549
HapII CCGG 1 cut(s) 162
HgaI GACGC 1 cut(s) 236
Hin1II CATG 2 cut(s) 231, 601
HinfI GANTC 1 cut(s) 239
HpaII CCGG 1 cut(s) 162
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 2 cut(s) 138, 394
Hpy188I TCNGA 2 cut(s) 208, 284
Hpy188III TCNNGA 2 cut(s) 236, 598
Hpy8I GTNNAC 2 cut(s) 138, 394
HpyAV CCTTC 3 cut(s) 279, 395, 511
HpyCH4III ACNGT 3 cut(s) 116, 560, 589
HpyCH4V TGCA 8 cut(s) 19, 138, 231, 329, 341, 386, 419, 584
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 326, 411
Hsp92II CATG 2 cut(s) 231, 601
LmnI GCTCC 1 cut(s) 15
LpnPI CCDG 8 cut(s) 27, 102, 161, 175, 221, 356, 432, 482
Lsp1109I GCAGC 2 cut(s) 477, 552
LweI GCATC 2 cut(s) 477, 571
MaeI CTAG 2 cut(s) 68, 429
MboII GAAGA 2 cut(s) 89, 167
MfeI CAATTG 1 cut(s) 330
MhlI GDGCHC 2 cut(s) 140, 192
MluCI AATT 2 cut(s) 330, 422
MmeI TCCRAC 2 cut(s) 147, 283
MnlI CCTC 7 cut(s) 75, 126, 151, 163, 266, 323, 483
MroXI GAANNNNTTC 1 cut(s) 219
MseI TTAA 1 cut(s) 381
MspI CCGG 1 cut(s) 162
MspR9I CCNGG 1 cut(s) 163
MunI CAATTG 1 cut(s) 330
MwoI GCNNNNNNNGC 3 cut(s) 16, 326, 411
NciI CCSGG 1 cut(s) 163
NlaIII CATG 2 cut(s) 231, 601
NlaIV GGNNCC 2 cut(s) 11, 406
PagI TCATGA 1 cut(s) 597
PceI AGGCCT 1 cut(s) 414
PdmI GAANNNNTTC 1 cut(s) 219
PfeI GAWTC 1 cut(s) 239
PkrI GCNGC 3 cut(s) 467, 551, 567
PshAI GACNNNNGTC 1 cut(s) 542
PshBI ATTAAT 1 cut(s) 381
PspFI CCCAGC 1 cut(s) 446
PspN4I GGNNCC 2 cut(s) 11, 406
PspPI GGNCC 1 cut(s) 305
RsaI GTAC 2 cut(s) 72, 395
RsaNI GTAC 2 cut(s) 71, 394
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 3 cut(s) 466, 550, 566
Sau96I GGNCC 1 cut(s) 305
ScrFI CCNGG 1 cut(s) 163
SduI GDGCHC 2 cut(s) 140, 192
SetI ASST 6 cut(s) 67, 137, 258, 372, 523, 530
SfaNI GCATC 2 cut(s) 477, 571
SinI GGWCC 1 cut(s) 305
Sse9I AATT 2 cut(s) 330, 422
SseBI AGGCCT 1 cut(s) 414
SsiI CCGC 1 cut(s) 550
SspMI CTAG 2 cut(s) 68, 429
StuI AGGCCT 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 161
StyI CCWWGG 1 cut(s) 569
TaaI ACNGT 3 cut(s) 116, 560, 589
TasI AATT 2 cut(s) 330, 422
TatI WGTACW 1 cut(s) 70
TauI GCSGC 1 cut(s) 552
TfiI GAWTC 1 cut(s) 239
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 2 cut(s) 96, 563
TseI GCWGC 2 cut(s) 465, 565
TspDTI ATGAA 3 cut(s) 48, 504, 586
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 2 cut(s) 96, 563
VneI GTGCAC 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 305
VspI ATTAAT 1 cut(s) 381
XapI RAATTY 1 cut(s) 422
XmnI GAANNNNTTC 1 cut(s) 219
XspI CTAG 2 cut(s) 68, 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.