Rorug03G0009500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003540
Physical Location & Seq
Forward (+)
698477 .. 699432
956 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0009500.1

Sequence Viewer

Length: 369 bp
ATGGCAAGCCTCACCACCAAACTTGGAGCAATTGCAAGGACTCCAACTTCTAGGATTTTTTCGAGACGGATTCAAGTGGGTTCGCAGCAAGGAAGAGACGCACTACAACAAGGAGCAAATGAAGCTAGGAATACTGCTGAACAGGTGGCAAATCAGAGCAATGATATGGCTGGGAAGGTGTCTGCAACAGCACAAGATGTGACAGAGAAAGCAAAGCAGACAGTGCAGGATGCATGGGGCTCAGCTAAGGACACTGCCCAGAAGGCGGCCGACAACGTCATGGGAAAGGCACAAGAATCAAAGGAGTTCGTCAAAGAAAATGCAGAGCAGGTCAAGAAAAGCATGAACACCAAGGGTCCTATCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

122

Amino Acids

13.05

Weight (kDa)

9.69

Isoelectric Point (pI)

31.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 319
AciI CCGC 1 cut(s) 266
AcoI YGGCCR 1 cut(s) 267
AfiI CCNNNNNNNGG 1 cut(s) 265
AgsI TTSAA 1 cut(s) 74
AluBI AGCT 2 cut(s) 125, 245
AluI AGCT 2 cut(s) 125, 245
Alw26I GTCTC 2 cut(s) 58, 90
AoxI GGCC 1 cut(s) 267
ApeKI GCWGC 1 cut(s) 85
AspS9I GGNCC 1 cut(s) 356
AsuHPI GGTGA 1 cut(s) 4
AvaII GGWCC 1 cut(s) 356
BanII GRGCYC 1 cut(s) 242
BbvI GCAGC 1 cut(s) 97
BcoDI GTCTC 2 cut(s) 58, 90
BfaI CTAG 2 cut(s) 51, 126
BfuAI ACCTGC 1 cut(s) 319
BglI GCCNNNNNGGC 1 cut(s) 263
BisI GCNGC 2 cut(s) 86, 267
BlpI GCTNAGC 1 cut(s) 241
BlsI GCNGC 2 cut(s) 87, 268
Bme18I GGWCC 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 1 cut(s) 357
BmsI GCATC 1 cut(s) 220
Bpu10I CCTNAGC 1 cut(s) 246
Bpu1102I GCTNAGC 1 cut(s) 241
BsaJI CCNNGG 1 cut(s) 351
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse3DI GCAATG 1 cut(s) 166
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 1 cut(s) 235
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMI GCAATG 1 cut(s) 166
BseMII CTCAG 1 cut(s) 255
BseX3I CGGCCG 1 cut(s) 267
BseXI GCAGC 1 cut(s) 97
BseYI CCCAGC 1 cut(s) 170
BsgI GTGCAG 1 cut(s) 245
Bsh1285I CGRYCG 1 cut(s) 270
BshFI GGCC 1 cut(s) 269
BsiEI CGRYCG 1 cut(s) 270
BslI CCNNNNNNNGG 1 cut(s) 265
BsmAI GTCTC 2 cut(s) 58, 90
BsmBI CGTCTC 2 cut(s) 58, 90
BsnI GGCC 1 cut(s) 269
Bsp1286I GDGCHC 1 cut(s) 242
Bsp1720I GCTNAGC 1 cut(s) 241
BspACI CCGC 1 cut(s) 266
BspANI GGCC 1 cut(s) 269
BspCNI CTCAG 1 cut(s) 254
BspLI GGNNCC 1 cut(s) 357
BspMI ACCTGC 1 cut(s) 319
BsrDI GCAATG 1 cut(s) 166
BssECI CCNNGG 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 1 cut(s) 88
BstAPI GCANNNNNTGC 1 cut(s) 223
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 2 cut(s) 241, 246
BstF5I GGATG 1 cut(s) 235
BstMAI GTCTC 2 cut(s) 58, 90
BstMCI CGRYCG 1 cut(s) 270
BstMWI GCNNNNNNNGC 3 cut(s) 122, 223, 263
BstV1I GCAGC 1 cut(s) 97
BstZI CGGCCG 1 cut(s) 267
BsuRI GGCC 1 cut(s) 269
BtsCI GGATG 1 cut(s) 235
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 2 cut(s) 228, 252
BveI ACCTGC 1 cut(s) 319
Cac8I GCNNGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 356
CseI GACGC 1 cut(s) 107
CspCI CAANNNNNGTGG 2 cut(s) 4, 39
CviAII CATG 3 cut(s) 234, 280, 343
CviJI RGCY 6 cut(s) 9, 125, 170, 240, 245, 269
CviKI_1 RGCY 6 cut(s) 9, 125, 170, 240, 245, 269
DdeI CTNAG 2 cut(s) 241, 246
EaeI YGGCCR 1 cut(s) 267
EagI CGGCCG 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 88
EarI CTCTTC 1 cut(s) 88
EclXI CGGCCG 1 cut(s) 267
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 242
Eco47I GGWCC 1 cut(s) 356
Eco52I CGGCCG 1 cut(s) 267
EcoO109I RGGNCCY 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 351
EcoT22I ATGCAT 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 242
ErhI CCWWGG 1 cut(s) 351
Esp3I CGTCTC 2 cut(s) 58, 90
FaeI CATG 3 cut(s) 237, 283, 346
FaiI YATR 4 cut(s) 167, 235, 281, 344
FatI CATG 3 cut(s) 233, 279, 342
Fnu4HI GCNGC 2 cut(s) 86, 267
FokI GGATG 1 cut(s) 242
FriOI GRGCYC 1 cut(s) 242
Fsp4HI GCNGC 2 cut(s) 86, 267
FspBI CTAG 2 cut(s) 51, 126
GluI GCNGC 2 cut(s) 86, 267
GsaI CCCAGC 1 cut(s) 174
HaeIII GGCC 1 cut(s) 269
HgaI GACGC 1 cut(s) 107
Hin1II CATG 3 cut(s) 237, 283, 346
HinfI GANTC 3 cut(s) 40, 70, 296
HphI GGTGA 1 cut(s) 4
Hpy188I TCNGA 1 cut(s) 156
Hpy188III TCNNGA 2 cut(s) 63, 334
HpyAV CCTTC 2 cut(s) 169, 256
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 5 cut(s) 35, 185, 226, 233, 323
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 223, 263
HpyF3I CTNAG 2 cut(s) 241, 246
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 3 cut(s) 237, 283, 346
LmnI GCTCC 2 cut(s) 26, 113
LpnPI CCDG 5 cut(s) 128, 156, 212, 272, 314
Lsp1109I GCAGC 1 cut(s) 97
LweI GCATC 1 cut(s) 220
MaeI CTAG 2 cut(s) 51, 126
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 1 cut(s) 199
MboII GAAGA 1 cut(s) 105
MfeI CAATTG 1 cut(s) 30
MhlI GDGCHC 1 cut(s) 242
MluCI AATT 2 cut(s) 30, 364
MlyI GAGTC 1 cut(s) 34
MmeI TCCRAC 1 cut(s) 68
MnlI CCTC 1 cut(s) 20
Mph1103I ATGCAT 1 cut(s) 235
MseI TTAA 1 cut(s) 367
MunI CAATTG 1 cut(s) 30
MwoI GCNNNNNNNGC 3 cut(s) 122, 223, 263
NlaIII CATG 3 cut(s) 237, 283, 346
NlaIV GGNNCC 1 cut(s) 357
NmuCI GTSAC 1 cut(s) 199
NsiI ATGCAT 1 cut(s) 235
PfeI GAWTC 2 cut(s) 70, 296
PflFI GACNNNGTC 1 cut(s) 275
PkrI GCNGC 2 cut(s) 87, 268
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
PpuMI RGGWCCY 1 cut(s) 356
Psp5II RGGWCCY 1 cut(s) 356
PspFI CCCAGC 1 cut(s) 170
PspN4I GGNNCC 1 cut(s) 357
PspPI GGNCC 1 cut(s) 356
PspPPI RGGWCCY 1 cut(s) 356
PsyI GACNNNGTC 1 cut(s) 275
SaqAI TTAA 1 cut(s) 367
SatI GCNGC 2 cut(s) 86, 267
Sau96I GGNCC 1 cut(s) 356
SchI GAGTC 1 cut(s) 34
SduI GDGCHC 1 cut(s) 242
SetI ASST 6 cut(s) 127, 147, 180, 247, 279, 333
SfaNI GCATC 1 cut(s) 220
SinI GGWCC 1 cut(s) 356
Sse9I AATT 2 cut(s) 30, 364
SsiI CCGC 1 cut(s) 266
SspMI CTAG 2 cut(s) 51, 126
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 1 cut(s) 223
TaiI ACGT 1 cut(s) 279
TaqI TCGA 1 cut(s) 62
TasI AATT 2 cut(s) 30, 364
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 2 cut(s) 70, 296
Tru1I TTAA 1 cut(s) 367
Tru9I TTAA 1 cut(s) 367
TscAI CASTG 2 cut(s) 228, 259
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 1 cut(s) 85
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 2 cut(s) 135, 359
TspGWI ACGGA 1 cut(s) 82
TspRI CASTG 2 cut(s) 228, 259
Tth111I GACNNNGTC 1 cut(s) 275
VpaK11BI GGWCC 1 cut(s) 356
XspI CTAG 2 cut(s) 51, 126
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.