Rorug03G0038400

NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002333
Physical Location & Seq
Forward (+)
3012328 .. 3017987
5660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0038400.1

Sequence Viewer

Length: 966 bp
ATGGAGGGCCAATGTGCAATCAAGAAAGATGTCACTGAATTAATCGGAAATACCCCAATGGTATACCTGAACAATGTCGTGGAAGGTTGTGTAGCTCAAATTGCTGCCAAGCTCGAGATGATGGAACCCTGCTGTAGTGTGAAAGACAGGATTGCATATAGTATGATCAAGGACGCAGAGGATAAAGGTCTAATAACTCCGGGGAAGACTGTTCTCATTGAGCCCACAAGTGGAAACACTGGCATTGGATTGGCATTCATCGCAGCAAGCAAGGGTTACAAGCTGAAGTTAGTCATGCCATCTGTAATGAGTCTTGAAAAAAGAATTGTGCTCTTAGCTTTTGGAGCTGAGGTTTACCTCACAGATTGGGCCAAAGGCTTTAAGGGAAGTCTTGATAAGGTTGAGGAGTTGCTACGTGACACACCTAATAGTCATACGCTTCAGCAATTTGAAAACCCTGCCAATCCAAAGATTCATTATGAAACCACCGGCCCGGAGATATGGAGAGACTCGGCAGGGAAAGTTGATATCTTGGTTTCGGGGATAGGAACTGGTGGTACAGTAACTGGTGTAGGGAAGTTTCTTAAAGAGCAAAATCCAGAGATCAAGGTTTACGGTGTAGAACCAATTGAGAGTGCAGTCTTGAATGGTGGTCCACCTGGGAAGCATCTGATCCAAGGACTTGGTGCCGGCATCATACCTCAAGTGCTCGATGTTAACCTGCCTGATAAAGTTATTACAGTATCCAGTGAGGAATCTATCGAGACTGCTAAGCTGCTTGCCCTAAAAGGTCTTTTGGTGGGGATCTCATCTGGTGCTGCAGCAGCAGTAGCAATAAAGTTGGCAAAGAAGCAGGAAAATGCTGGAAAACTAATAGTTGTCGTGTTCCCCAGTTTCGGAGAGCGGTATCTGTCTTCTCCACTTTTTGATACCATTAGGCATGAGGCAGAAAAAATGACTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
MWFE

Protein Analysis

321

Amino Acids

34.31

Weight (kDa)

5.93

Isoelectric Point (pI)

22.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 11 - 297 1.3e-69 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 729
AccB1I GGYRCC 1 cut(s) 686
AccBSI CCGCTC 1 cut(s) 904
AccI GTMKAC 1 cut(s) 63
AciI CCGC 1 cut(s) 904
AclWI GGATC 2 cut(s) 667, 812
AcuI CTGAAG 2 cut(s) 305, 425
AfaI GTAC 1 cut(s) 559
AfiI CCNNNNNNNGG 2 cut(s) 230, 896
AgsI TTSAA 3 cut(s) 317, 452, 646
AjnI CCWGG 1 cut(s) 658
AluBI AGCT 6 cut(s) 95, 112, 283, 338, 347, 775
AluI AGCT 6 cut(s) 95, 112, 283, 338, 347, 775
Alw21I GWGCWC 2 cut(s) 333, 711
Alw26I GTCTC 2 cut(s) 501, 758
AlwI GGATC 2 cut(s) 667, 812
AlwNI CAGNNNCTG 1 cut(s) 566
Ama87I CYCGRG 1 cut(s) 113
AoxI GGCC 3 cut(s) 7, 369, 490
ApeKI GCWGC 6 cut(s) 104, 263, 775, 818, 821, 824
AseI ATTAAT 1 cut(s) 41
AspS9I GGNCC 4 cut(s) 7, 369, 491, 653
AsuC2I CCSGG 2 cut(s) 201, 494
AvaI CYCGRG 1 cut(s) 113
AvaII GGWCC 1 cut(s) 653
BanI GGYRCC 1 cut(s) 686
BanII GRGCYC 1 cut(s) 225
BbsI GAAGAC 2 cut(s) 212, 906
Bbv12I GWGCWC 2 cut(s) 333, 711
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 6 cut(s) 91, 275, 762, 805, 833, 836
BccI CCATC 2 cut(s) 115, 307
BciT130I CCWGG 1 cut(s) 660
BciVI GTATCC 1 cut(s) 754
BclI TGATCA 1 cut(s) 165
BcnI CCSGG 2 cut(s) 201, 494
BcoDI GTCTC 2 cut(s) 501, 758
BfmI CTRYAG 2 cut(s) 133, 819
BfuAI ACCTGC 1 cut(s) 729
BfuI GTATCC 1 cut(s) 754
BisI GCNGC 6 cut(s) 105, 264, 776, 819, 822, 825
BlpI GCTNAGC 1 cut(s) 771
BlsI GCNGC 6 cut(s) 106, 265, 777, 820, 823, 826
Bme1390I CCNGG 3 cut(s) 201, 494, 660
Bme18I GGWCC 1 cut(s) 653
BmeT110I CYCGRG 1 cut(s) 113
BmgT120I GGNCC 4 cut(s) 7, 369, 491, 653
BmiI GGNNCC 2 cut(s) 126, 688
BmrFI CCNGG 3 cut(s) 201, 494, 660
BmrI ACTGGG 1 cut(s) 885
BmsI GCATC 2 cut(s) 676, 702
BmuI ACTGGG 1 cut(s) 885
BpiI GAAGAC 2 cut(s) 212, 906
Bpu10I CCTNAGC 1 cut(s) 348
Bpu1102I GCTNAGC 1 cut(s) 771
BpuEI CTTGAG 1 cut(s) 687
BpuMI CCSGG 2 cut(s) 201, 494
BsaAI YACGTR 1 cut(s) 416
BsaJI CCNNGG 3 cut(s) 200, 659, 676
BsaXI ACNNNNNCTCC 2 cut(s) 336, 366
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 896
Bse118I RCCGGY 2 cut(s) 488, 689
Bse1I ACTGG 5 cut(s) 244, 556, 571, 747, 891
BseBI CCWGG 1 cut(s) 660
BseDI CCNNGG 3 cut(s) 200, 659, 676
BseLI CCNNNNNNNGG 2 cut(s) 230, 896
BseMII CTCAG 1 cut(s) 339
BseNI ACTGG 5 cut(s) 244, 556, 571, 747, 891
BseRI GAGGAG 1 cut(s) 419
BseXI GCAGC 6 cut(s) 91, 275, 762, 805, 833, 836
BsgI GTGCAG 1 cut(s) 657
BshFI GGCC 3 cut(s) 9, 371, 492
BshNI GGYRCC 1 cut(s) 686
BsiHKAI GWGCWC 2 cut(s) 333, 711
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 4 cut(s) 200, 489, 494, 690
BslI CCNNNNNNNGG 2 cut(s) 230, 896
BsmAI GTCTC 2 cut(s) 501, 758
BsmI GAATGC 1 cut(s) 254
BsnI GGCC 3 cut(s) 9, 371, 492
BsoBI CYCGRG 1 cut(s) 113
Bsp1286I GDGCHC 3 cut(s) 225, 333, 711
Bsp143I GATC 4 cut(s) 165, 603, 672, 804
Bsp1720I GCTNAGC 1 cut(s) 771
BspACI CCGC 1 cut(s) 904
BspANI GGCC 3 cut(s) 9, 371, 492
BspCNI CTCAG 1 cut(s) 340
BspLI GGNNCC 2 cut(s) 126, 688
BspMAI CTGCAG 1 cut(s) 823
BspMI ACCTGC 1 cut(s) 729
BspPI GGATC 2 cut(s) 667, 812
BspT107I GGYRCC 1 cut(s) 686
BsrBI CCGCTC 1 cut(s) 904
BsrFI RCCGGY 2 cut(s) 488, 689
BsrI ACTGG 5 cut(s) 244, 556, 571, 747, 891
BssAI RCCGGY 2 cut(s) 488, 689
BssECI CCNNGG 3 cut(s) 200, 659, 676
BssMI GATC 4 cut(s) 165, 603, 672, 804
BssNAI GTATAC 1 cut(s) 64
BssT1I CCWWGG 1 cut(s) 676
Bst1107I GTATAC 1 cut(s) 64
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 4 cut(s) 211, 562, 617, 742
BstBAI YACGTR 1 cut(s) 416
BstC8I GCNNGC 3 cut(s) 268, 691, 780
BstDEI CTNAG 3 cut(s) 334, 348, 771
BstKTI GATC 4 cut(s) 168, 606, 675, 807
BstMAI GTCTC 2 cut(s) 501, 758
BstMBI GATC 4 cut(s) 165, 603, 672, 804
BstMWI GCNNNNNNNGC 5 cut(s) 101, 260, 344, 824, 830
BstNI CCWGG 1 cut(s) 660
BstSCI CCNGG 3 cut(s) 199, 492, 658
BstSFI CTRYAG 2 cut(s) 133, 819
BstV1I GCAGC 6 cut(s) 91, 275, 762, 805, 833, 836
BstV2I GAAGAC 2 cut(s) 212, 906
BstX2I RGATCY 1 cut(s) 804
BstXI CCANNNNNNTGG 1 cut(s) 683
BstYI RGATCY 1 cut(s) 804
BstZ17I GTATAC 1 cut(s) 64
BsuI GTATCC 1 cut(s) 754
BsuRI GGCC 3 cut(s) 9, 371, 492
BtgZI GCGATG 1 cut(s) 244
BtsIMutI CAGTG 3 cut(s) 33, 237, 754
BveI ACCTGC 1 cut(s) 729
Cac8I GCNNGC 3 cut(s) 268, 691, 780
CaiI CAGNNNCTG 1 cut(s) 566
Cfr10I RCCGGY 2 cut(s) 488, 689
Cfr13I GGNCC 4 cut(s) 7, 369, 491, 653
CseI GACGC 1 cut(s) 182
Csp6I GTAC 1 cut(s) 558
CviAII CATG 2 cut(s) 295, 941
CviQI GTAC 1 cut(s) 558
DdeI CTNAG 3 cut(s) 334, 348, 771
DpnI GATC 4 cut(s) 167, 605, 674, 806
DpnII GATC 4 cut(s) 165, 603, 672, 804
Eco130I CCWWGG 1 cut(s) 676
Eco24I GRGCYC 1 cut(s) 225
Eco32I GATATC 1 cut(s) 529
Eco47I GGWCC 1 cut(s) 653
Eco57I CTGAAG 2 cut(s) 305, 425
Eco88I CYCGRG 1 cut(s) 113
EcoRII CCWGG 1 cut(s) 658
EcoRV GATATC 1 cut(s) 529
EcoT14I CCWWGG 1 cut(s) 676
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 676
FaeI CATG 2 cut(s) 298, 944
FatI CATG 2 cut(s) 294, 940
FbaI TGATCA 1 cut(s) 165
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 6 cut(s) 105, 264, 776, 819, 822, 825
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 6 cut(s) 105, 264, 776, 819, 822, 825
GluI GCNGC 6 cut(s) 105, 264, 776, 819, 822, 825
HaeIII GGCC 3 cut(s) 9, 371, 492
HapII CCGG 4 cut(s) 200, 489, 494, 690
HgaI GACGC 1 cut(s) 182
Hin1II CATG 2 cut(s) 298, 944
HincII GTYRAC 1 cut(s) 718
HindII GTYRAC 1 cut(s) 718
HinfI GANTC 4 cut(s) 310, 472, 509, 755
HpaI GTTAAC 1 cut(s) 718
HpaII CCGG 4 cut(s) 200, 489, 494, 690
Hpy166II GTNNAC 5 cut(s) 64, 355, 613, 656, 718
Hpy188I TCNGA 3 cut(s) 47, 672, 899
Hpy188III TCNNGA 7 cut(s) 22, 115, 314, 392, 599, 643, 763
Hpy8I GTNNAC 5 cut(s) 64, 355, 613, 656, 718
HpyAV CCTTC 1 cut(s) 77
HpyCH4III ACNGT 4 cut(s) 211, 562, 617, 742
HpyCH4IV ACGT 1 cut(s) 415
HpyCH4V TGCA 4 cut(s) 17, 155, 638, 821
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 260, 344, 824, 830
HpyF3I CTNAG 3 cut(s) 334, 348, 771
HpySE526I ACGT 1 cut(s) 415
Hsp92II CATG 2 cut(s) 298, 944
KroI GCCGGC 1 cut(s) 689
KroNI GCCGGC 1 cut(s) 691
Ksp22I TGATCA 1 cut(s) 165
KspAI GTTAAC 1 cut(s) 718
Kzo9I GATC 4 cut(s) 165, 603, 672, 804
LmnI GCTCC 1 cut(s) 344
Lsp1109I GCAGC 6 cut(s) 91, 275, 762, 805, 833, 836
LweI GCATC 2 cut(s) 676, 702
MaeII ACGT 1 cut(s) 415
MaeIII GTNAC 4 cut(s) 31, 275, 416, 562
MalI GATC 4 cut(s) 167, 605, 674, 806
MbiI CCGCTC 1 cut(s) 904
MboI GATC 4 cut(s) 165, 603, 672, 804
MboII GAAGA 2 cut(s) 217, 906
MfeI CAATTG 1 cut(s) 627
MflI RGATCY 1 cut(s) 804
MhlI GDGCHC 3 cut(s) 225, 333, 711
MluCI AATT 5 cut(s) 38, 99, 324, 446, 627
MlyI GAGTC 2 cut(s) 319, 503
MnlI CCTC 7 cut(s) 172, 343, 368, 397, 711, 745, 937
MroNI GCCGGC 1 cut(s) 689
MseI TTAA 4 cut(s) 41, 381, 585, 717
MspI CCGG 4 cut(s) 200, 489, 494, 690
MspR9I CCNGG 3 cut(s) 201, 494, 660
MunI CAATTG 1 cut(s) 627
Mva1269I GAATGC 1 cut(s) 254
MvaI CCWGG 1 cut(s) 660
MwoI GCNNNNNNNGC 5 cut(s) 101, 260, 344, 824, 830
NaeI GCCGGC 1 cut(s) 691
NciI CCSGG 2 cut(s) 201, 494
NdeII GATC 4 cut(s) 165, 603, 672, 804
NgoMIV GCCGGC 1 cut(s) 689
NlaIII CATG 2 cut(s) 298, 944
NlaIV GGNNCC 2 cut(s) 126, 688
NmeAIII GCCGAG 1 cut(s) 491
NmuCI GTSAC 2 cut(s) 31, 416
PaeR7I CTCGAG 1 cut(s) 113
PctI GAATGC 1 cut(s) 254
PdiI GCCGGC 1 cut(s) 691
PfeI GAWTC 2 cut(s) 472, 755
PkrI GCNGC 6 cut(s) 106, 265, 777, 820, 823, 826
PleI GAGTC 2 cut(s) 318, 503
PpsI GAGTC 2 cut(s) 318, 503
Ppu21I YACGTR 1 cut(s) 416
PshBI ATTAAT 1 cut(s) 41
Psp6I CCWGG 1 cut(s) 658
PspGI CCWGG 1 cut(s) 658
PspN4I GGNNCC 2 cut(s) 126, 688
PspPI GGNCC 4 cut(s) 7, 369, 491, 653
PsrI GAACNNNNNNTAC 2 cut(s) 541, 573
PstI CTGCAG 1 cut(s) 823
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 804
RsaI GTAC 1 cut(s) 559
RsaNI GTAC 1 cut(s) 558
SaqAI TTAA 4 cut(s) 41, 381, 585, 717
SatI GCNGC 6 cut(s) 105, 264, 776, 819, 822, 825
Sau3AI GATC 4 cut(s) 165, 603, 672, 804
Sau96I GGNCC 4 cut(s) 7, 369, 491, 653
SchI GAGTC 2 cut(s) 319, 503
ScrFI CCNGG 3 cut(s) 201, 494, 660
SduI GDGCHC 3 cut(s) 225, 333, 711
SfaNI GCATC 2 cut(s) 676, 702
SfcI CTRYAG 2 cut(s) 133, 819
Sfr274I CTCGAG 1 cut(s) 113
SinI GGWCC 1 cut(s) 653
SlaI CTCGAG 1 cut(s) 113
SmlI CTYRAG 2 cut(s) 113, 702
SmoI CTYRAG 2 cut(s) 113, 702
Sse9I AATT 5 cut(s) 38, 99, 324, 446, 627
SsiI CCGC 1 cut(s) 904
StyD4I CCNGG 3 cut(s) 199, 492, 658
StyI CCWWGG 1 cut(s) 676
TaaI ACNGT 4 cut(s) 211, 562, 617, 742
TaiI ACGT 1 cut(s) 418
TaqI TCGA 3 cut(s) 114, 711, 762
TasI AATT 5 cut(s) 38, 99, 324, 446, 627
TfiI GAWTC 2 cut(s) 472, 755
Tru1I TTAA 4 cut(s) 41, 381, 585, 717
Tru9I TTAA 4 cut(s) 41, 381, 585, 717
TscAI CASTG 3 cut(s) 40, 244, 754
TseFI GTSAC 2 cut(s) 31, 416
TseI GCWGC 6 cut(s) 104, 263, 775, 818, 821, 824
Tsp45I GTSAC 2 cut(s) 31, 416
TspDTI ATGAA 3 cut(s) 247, 464, 495
TspRI CASTG 3 cut(s) 40, 244, 754
VpaK11BI GGWCC 1 cut(s) 653
VspI ATTAAT 1 cut(s) 41
XhoI CTCGAG 1 cut(s) 113
XmiI GTMKAC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.