Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0008170

Physical Location & Seq

Genomic Coordinates Forward (+)

15179302 .. 15186867

Gene Structure 7566 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0178600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2124 bp

ATGGACTGGGCTTTTGAAACATGGCCGTGTCTAAAACCCGACCCGAACTCCAAGCTTCTAATATCGATTCGTTACAAACCCGAGTCTCACCGCCAAGGCATGGGACGCAGCAGCAGGAGTAGGAAGGAGGCCTCATCCAGTCGACGCAGACGAAAGTCCGACGACTCCGACTCCGACTCCGACTCCGATTCGTACACCGACTCCGACGATTCCGACAGCCTCGACTCCTCTCCGGCGAGGAGTTCCGGCAAGCGCAAGGAGCGAAGCAGTCGCAGCAGAAAGAGCAGCAGCCGCCGCCGGAACCACTCCGACGACGAGTCGTATGAAAGCGACGACAGCGACCGCAAGAAGAAGAGGTCGTCGAGGAACGTCACCGAGGAGCAAATCGCCGAGTACTTGGCTAAGAAGGCACAGAAAAAGGCCGCTCGAGTAGCTAAGAAGCTGAAATCGAAGACGGTTTCGGGTTATTCCAATGATTCGAATCCCTTTGGTGATTCCAATCTCAATGAGACATTTGTATGGAGAAAGAAGATTGAGCGTGATGTCTCCCACGGCGTGCCACTCGACGTGTATTCAGTTAAAGCTGAGAAGCAAAGACAGAGAGACAGGATGGCTGAGATAGAAAAGGTTAAGAAGAGAAGGGAGGAAAGAGCACTTGAAAAAGCTCAACATGAGGAAGAAATGGCGCTGCTTGCCAGAGAACGCGCTCGGGCTGAGTTCCAAGACTGGGAGAAAAAAGAAGAAGAGTTCCACTTTGATCAAAGCAAAATGAGGTCAGAGATCAGATTGCGTGAAGGGCGTATCAAGCCAATTGATGTACTTTCCAAGCATCTCGATGGCTCAGATGATTTAGATATTGAGATAAATGAGCCATACATGGTTTTCAAGGGCTTGACGGTAAAGGAAATGGAAGAGCTTCGAGATGACATCAAAATGCATCTTGATTTGGACAGGGCAACCCCTACACATATAGAATACTGGGAGGCACTTTTGGTGGTTTGCGATTGGGAGCTATCTGAAGCTCGGAAAAAGGATGCTTTGGATCGAGCTAGGGTTCGTGGGGAGGAACCTCCTGCCGAGTTACTTGCTGAACAAAGAGGCATGCATTCTAGCATTGAAGCAGATGTCAAGAATCTCTTGGAAGGGAAGACTTACAGTCAGTTGGAGGCATTACAGTCTGAAATTGAGTCACAGATGCGTTCTGGTACAGCCAAGGTCGTTGAGTATTGGGAGGCTGTTCTTACACGCCTCCATATATTCAAGGCAAAGGCCTGTTTGAAAGAAATCCATGCTAAAATGTTGCACAAGCATCTGCAACGCCTTGAGGGACGAGAGGATGATGAAGAGAAATTGGATATGCCCGATGACTTGCAACCTGAAGAGGAGAGTGAGCCTGATGCTAATGGTGCTGAAACATATTCACCAGAACCTGTAGAGGAGGAGATCCACATTGCTGAAGAAGAGGCTGAAGAGGCCGGTTCATTTTCACCTGAACTATTACATGGAGATGAAAATGAGGAAGTGATGGACCCTGAAGAGGATAGGGCTATACTGGAACGCAAGCGTATGGCCGTATTAGAAGAACAACAAAGACGAATTCAAGAGGCTATGGCATCAAAGCCAGCTCCACCATCAGAAGATAACTTTGAGATGAAGGCTGCCATGAAAGCCATGGGTGCCATGGAGGAAGGTGATGCAATATTTGGCTCTGGTTCTGAAGTGAACCTTGACTCACAGGTTTACTGGTGGCACGACAAGTACCGACCTAGAAAGCCGAAATATTTCAACCGTGTTCACACAGGATATGAATGGAACAAGTACAATCAGACCCACTATGATCACGATAACCCACCTCCAAAGATTGTGCAAGGATATAAATTCAACATCTTCTATCCAGACCTTGTTGACAAGATAAAGGCTCCAACTTATACTATTGAGAAGGATGGAAACAGCAGCGAGACCTGCATTATAAGGTTTCATGCAGGGCCACCTTACGAAGACATTGCATTCCGTATAGTGAACAAAGAATGGGAATACTCTCACAAGAAGGGGTTTAAATGCACATTCGAGCGTGGAATTTTGCACGTATACTTTAACTTCAAACGATACCGGTACCGCAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000913 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005819 GO:0005829 GO:0005856 GO:0005871 GO:0005875 GO:0005886 GO:0006810 GO:0006928 GO:0006996 GO:0007017 GO:0007018 GO:0007049 GO:0007097 GO:0008017 GO:0008092 GO:0008150 GO:0009504 GO:0009507 GO:0009524 GO:0009526 GO:0009536 GO:0009657 GO:0009658 GO:0009902 GO:0009903 GO:0009904 GO:0009941 GO:0009987 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019750 GO:0022402 GO:0022607 GO:0030029 GO:0030048 GO:0030705 GO:0031022 GO:0031967 GO:0031975 GO:0032506 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0051179 GO:0051234 GO:0051301 GO:0051640 GO:0051641 GO:0051644 GO:0051647 GO:0051649 GO:0051656 GO:0051667 GO:0061640 GO:0071840 GO:0071944 GO:0099515 GO:1902410 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

707

Amino Acids

82.69

Weight (kDa)

5.71

Isoelectric Point (pI)

69.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cactin_mid	PF10312	233 - 430	3.5e-62	Conserved mid region of cactin
CactinC_cactus	PF09732	583 - 707	3e-63	Cactus-binding C-terminus of cactin protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008170)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr1g0360281
rosa_laevigata	RLG00000018534 RLG00000022811 RLG00000029560
rosa_multiflora	Rmu_co8335897.1_g000001 Rmu_sc0000082.1_g000021
rosa_roxburghii	Rroxscaffold_4G00320420 Rroxscaffold_5G00346690
rosa_rugosa	Rorug03G0178600 Rorug05G0448700.1 Rorug06G0217900 Rorug06G0217900
rosa_samantha	Rh2AG271400 Rh2AG444000 Rh2CG372200 Rh3BG356100 Rh4CG185900 Rh5AG263200 Rh5BG273100 Rh5CG578900 Rh5DG281100 Rh6AG351000 Rh6BG096600 Rh7AG137200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1970
AarI	CACCTGC	1 cut(s)	2109
Acc36I	ACCTGC	2 cut(s)	1970, 2109
Acc65I	GGTACC	1 cut(s)	2112
AccB1I	GGYRCC	2 cut(s)	1676, 2112
AccB7I	CCANNNNNTGG	1 cut(s)	100
AccBSI	CCGCTC	1 cut(s)	425
AccI	GTMKAC	2 cut(s)	142, 2088
AccII	CGCG	1 cut(s)	705
AciI	CCGC	6 cut(s)	91, 292, 295, 343, 423, 2116
AclWI	GGATC	2 cut(s)	1050, 1438
AcoI	YGGCCR	2 cut(s)	23, 1569
AcsI	RAATTY	3 cut(s)	1596, 1877, 2076
AcuI	CTGAAG	6 cut(s)	1038, 1398, 1476, 1488, 1554, 1737
AdeI	CACNNNGTG	1 cut(s)	556
AfaI	GTAC	7 cut(s)	194, 395, 819, 1207, 1760, 1820, 2114
AfiI	CCNNNNNNNGG	3 cut(s)	100, 727, 1537
AflIII	ACRYGT	1 cut(s)	567
AgeI	ACCGGT	1 cut(s)	2109
AhdI	GACNNNNNGTC	2 cut(s)	316, 1155
AjiI	CACGTC	1 cut(s)	568
Alw21I	GWGCWC	1 cut(s)	655
Alw26I	GTCTC	5 cut(s)	90, 503, 550, 597, 1952
AlwI	GGATC	2 cut(s)	1050, 1438
AlwNI	CAGNNNCTG	1 cut(s)	1430
Ama87I	CYCGRG	3 cut(s)	80, 426, 708
AoxI	GGCC	7 cut(s)	23, 129, 420, 1269, 1473, 1569, 1985
ApeKI	GCWGC	8 cut(s)	108, 111, 273, 285, 288, 688, 1658, 1953
ApoI	RAATTY	3 cut(s)	1596, 1877, 2076
ArsI	GACNNNNNNTTYG	1 cut(s)	28
AsiGI	ACCGGT	1 cut(s)	2109
Asp700I	GAANNNNTTC	2 cut(s)	915, 1781
Asp718I	GGTACC	1 cut(s)	2112
AspLEI	GCGC	3 cut(s)	255, 688, 707
AspS9I	GGNCC	2 cut(s)	1528, 1985
AsuHPI	GGTGA	6 cut(s)	80, 364, 503, 1413, 1479, 1703
AsuII	TTCGAA	1 cut(s)	479
AvaI	CYCGRG	3 cut(s)	80, 426, 708
AvaII	GGWCC	1 cut(s)	1528
BanI	GGYRCC	2 cut(s)	1676, 2112
BbsI	GAAGAC	3 cut(s)	458, 1154, 2004
Bbv12I	GWGCWC	1 cut(s)	655
BbvI	GCAGC	8 cut(s)	120, 123, 285, 297, 300, 675, 1645, 1965
BccI	CCATC	5 cut(s)	604, 830, 1519, 1639, 1937
BceAI	ACGGC	3 cut(s)	10, 568, 1556
BcgI	CGANNNNNNTGC	4 cut(s)	1067, 1101, 1985, 2019
BclI	TGATCA	2 cut(s)	757, 1837
BcoDI	GTCTC	5 cut(s)	90, 503, 550, 597, 1952
BfaI	CTAG	3 cut(s)	1050, 1110, 1767
BfmI	CTRYAG	1 cut(s)	1431
BfoI	RGCGCY	1 cut(s)	689
BfuAI	ACCTGC	2 cut(s)	1970, 2109
BmcAI	AGTACT	1 cut(s)	395
Bme18I	GGWCC	1 cut(s)	1528
BmeRI	GACNNNNNGTC	2 cut(s)	316, 1155
BmeT110I	CYCGRG	3 cut(s)	80, 426, 708
BmgBI	CACGTC	1 cut(s)	568
BmgT120I	GGNCC	2 cut(s)	1528, 1985
BmiI	GGNNCC	6 cut(s)	302, 1068, 1530, 1678, 1920, 2114
BmrI	ACTGGG	3 cut(s)	16, 736, 988
BmsI	GCATC	8 cut(s)	838, 946, 1024, 1185, 1318, 1387, 1622, 1684
BmuI	ACTGGG	3 cut(s)	16, 736, 988
BoxI	GACNNNNGTC	1 cut(s)	154
BpiI	GAAGAC	3 cut(s)	458, 1154, 2004
Bpu14I	TTCGAA	1 cut(s)	479
BpuEI	CTTGAG	1 cut(s)	1343
Bsa29I	ATCGAT	1 cut(s)	65
BsaAI	YACGTR	1 cut(s)	2086
BsaBI	GATNNNNATC	2 cut(s)	480, 498
BsaI	GGTCTC	1 cut(s)	1952
BsaJI	CCNNGG	6 cut(s)	94, 375, 550, 1212, 1671, 1680
BsaWI	WCCGGW	1 cut(s)	2109
Bsc4I	CCNNNNNNNGG	3 cut(s)	100, 727, 1537
Bse118I	RCCGGY	2 cut(s)	1475, 2109
Bse1I	ACTGG	6 cut(s)	11, 138, 731, 983, 1557, 1748
Bse3DI	GCAATG	2 cut(s)	1449, 2001
Bse8I	GATNNNNATC	2 cut(s)	480, 498
BseCI	ATCGAT	1 cut(s)	65
BseDI	CCNNGG	6 cut(s)	94, 375, 550, 1212, 1671, 1680
BseGI	GGATG	5 cut(s)	134, 615, 1039, 1342, 1948
BseJI	GATNNNNATC	2 cut(s)	480, 498
BseLI	CCNNNNNNNGG	3 cut(s)	100, 727, 1537
BseMI	GCAATG	2 cut(s)	1449, 2001
BseMII	CTCAG	4 cut(s)	576, 606, 705, 855
BseNI	ACTGG	6 cut(s)	11, 138, 731, 983, 1557, 1748
BseRI	GAGGAG	6 cut(s)	217, 253, 392, 1397, 1451, 1454
BseXI	GCAGC	8 cut(s)	120, 123, 285, 297, 300, 675, 1645, 1965
Bsh1236I	CGCG	1 cut(s)	705
Bsh1285I	CGRYCG	1 cut(s)	343
BshFI	GGCC	7 cut(s)	25, 131, 422, 1271, 1475, 1571, 1987
BshNI	GGYRCC	2 cut(s)	1676, 2112
BshTI	ACCGGT	1 cut(s)	2109
BshVI	ATCGAT	1 cut(s)	65
BsiEI	CGRYCG	1 cut(s)	343
BsiHKAI	GWGCWC	1 cut(s)	655
BsiHKCI	CYCGRG	3 cut(s)	80, 426, 708
BsiSI	CCGG	5 cut(s)	233, 246, 298, 1476, 2110
BslFI	GGGAC	2 cut(s)	117, 1341
BslI	CCNNNNNNNGG	3 cut(s)	100, 727, 1537
BsmAI	GTCTC	5 cut(s)	90, 503, 550, 597, 1952
BsmFI	GGGAC	2 cut(s)	117, 1341
BsmI	GAATGC	2 cut(s)	1105, 2006
BsnI	GGCC	7 cut(s)	25, 131, 422, 1271, 1475, 1571, 1987
Bso31I	GGTCTC	1 cut(s)	1952
BsoBI	CYCGRG	3 cut(s)	80, 426, 708
Bsp119I	TTCGAA	1 cut(s)	479
Bsp1286I	GDGCHC	1 cut(s)	655
Bsp143I	GATC	5 cut(s)	757, 780, 1042, 1443, 1837
Bsp19I	CCATGG	2 cut(s)	1671, 1680
BspACI	CCGC	6 cut(s)	91, 292, 295, 343, 423, 2116
BspANI	GGCC	7 cut(s)	25, 131, 422, 1271, 1475, 1571, 1987
BspCNI	CTCAG	4 cut(s)	577, 607, 706, 854
BspDI	ATCGAT	1 cut(s)	65
BspFNI	CGCG	1 cut(s)	705
BspLI	GGNNCC	6 cut(s)	302, 1068, 1530, 1678, 1920, 2114
BspMI	ACCTGC	2 cut(s)	1970, 2109
BspPI	GGATC	2 cut(s)	1050, 1438
BspQI	GCTCTTC	1 cut(s)	906
BspT104I	TTCGAA	1 cut(s)	479
BspT107I	GGYRCC	2 cut(s)	1676, 2112
BspTNI	GGTCTC	1 cut(s)	1952
BsrBI	CCGCTC	1 cut(s)	425
BsrDI	GCAATG	2 cut(s)	1449, 2001
BsrFI	RCCGGY	2 cut(s)	1475, 2109
BsrI	ACTGG	6 cut(s)	11, 138, 731, 983, 1557, 1748
BssAI	RCCGGY	2 cut(s)	1475, 2109
BssECI	CCNNGG	6 cut(s)	94, 375, 550, 1212, 1671, 1680
BssMI	GATC	5 cut(s)	757, 780, 1042, 1443, 1837
BssNAI	GTATAC	1 cut(s)	2089
BssT1I	CCWWGG	4 cut(s)	94, 1212, 1671, 1680
Bst1107I	GTATAC	1 cut(s)	2089
Bst4CI	ACNGT	5 cut(s)	457, 898, 1157, 1176, 1790
Bst6I	CTCTTC	9 cut(s)	347, 629, 738, 906, 1338, 1374, 1455, 1464, 1530
BstBAI	YACGTR	1 cut(s)	2086
BstBI	TTCGAA	1 cut(s)	479
BstC8I	GCNNGC	6 cut(s)	251, 557, 693, 1103, 1562, 1623
BstDEI	CTNAG	6 cut(s)	402, 435, 585, 615, 714, 841
BstDSI	CCRYGG	3 cut(s)	550, 1671, 1680
BstF5I	GGATG	5 cut(s)	134, 615, 1039, 1342, 1948
BstFNI	CGCG	1 cut(s)	705
BstH2I	RGCGCY	1 cut(s)	689
BstHHI	GCGC	3 cut(s)	255, 688, 707
BstKTI	GATC	5 cut(s)	760, 783, 1045, 1446, 1840
BstMAI	GTCTC	5 cut(s)	90, 503, 550, 597, 1952
BstMBI	GATC	5 cut(s)	757, 780, 1042, 1443, 1837
BstMCI	CGRYCG	1 cut(s)	343
BstNSI	RCATGY	1 cut(s)	1105
BstPAI	GACNNNNGTC	1 cut(s)	154
BstSFI	CTRYAG	1 cut(s)	1431
BstUI	CGCG	1 cut(s)	705
BstV1I	GCAGC	8 cut(s)	120, 123, 285, 297, 300, 675, 1645, 1965
BstV2I	GAAGAC	3 cut(s)	458, 1154, 2004
BstX2I	RGATCY	1 cut(s)	1443
BstYI	RGATCY	1 cut(s)	1443
BstZ17I	GTATAC	1 cut(s)	2089
Bsu15I	ATCGAT	1 cut(s)	65
BsuRI	GGCC	7 cut(s)	25, 131, 422, 1271, 1475, 1571, 1987
BsuTUI	ATCGAT	1 cut(s)	65
BtgI	CCRYGG	3 cut(s)	550, 1671, 1680
BtrI	CACGTC	1 cut(s)	568
BtsCI	GGATG	5 cut(s)	134, 615, 1039, 1342, 1948
BveI	ACCTGC	2 cut(s)	1970, 2109
Cac8I	GCNNGC	6 cut(s)	251, 557, 693, 1103, 1562, 1623
CaiI	CAGNNNCTG	1 cut(s)	1430
CfoI	GCGC	3 cut(s)	255, 688, 707
Cfr10I	RCCGGY	2 cut(s)	1475, 2109
Cfr13I	GGNCC	2 cut(s)	1528, 1985
ClaI	ATCGAT	1 cut(s)	65
CseI	GACGC	2 cut(s)	114, 153
Csp6I	GTAC	7 cut(s)	193, 394, 818, 1206, 1759, 1819, 2113
CspAI	ACCGGT	1 cut(s)	2109
CviQI	GTAC	7 cut(s)	193, 394, 818, 1206, 1759, 1819, 2113
DdeI	CTNAG	6 cut(s)	402, 435, 585, 615, 714, 841
DpnI	GATC	5 cut(s)	759, 782, 1044, 1445, 1839
DpnII	GATC	5 cut(s)	757, 780, 1042, 1443, 1837
DraI	TTTAAA	1 cut(s)	2056
DraIII	CACNNNGTG	1 cut(s)	556
DriI	GACNNNNNGTC	2 cut(s)	316, 1155
EaeI	YGGCCR	2 cut(s)	23, 1569
Eam1104I	CTCTTC	9 cut(s)	347, 629, 738, 906, 1338, 1374, 1455, 1464, 1530
Eam1105I	GACNNNNNGTC	2 cut(s)	316, 1155
EarI	CTCTTC	9 cut(s)	347, 629, 738, 906, 1338, 1374, 1455, 1464, 1530
Eco130I	CCWWGG	4 cut(s)	94, 1212, 1671, 1680
Eco147I	AGGCCT	2 cut(s)	131, 1271
Eco31I	GGTCTC	1 cut(s)	1952
Eco47I	GGWCC	1 cut(s)	1528
Eco57I	CTGAAG	6 cut(s)	1038, 1398, 1476, 1488, 1554, 1737
Eco88I	CYCGRG	3 cut(s)	80, 426, 708
EcoRI	GAATTC	1 cut(s)	1596
EcoT14I	CCWWGG	4 cut(s)	94, 1212, 1671, 1680
EcoT22I	ATGCAT	2 cut(s)	939, 1107
ErhI	CCWWGG	4 cut(s)	94, 1212, 1671, 1680
FalI	AAGNNNNNCTT	4 cut(s)	1121, 1153, 1710, 1742
FaqI	GGGAC	2 cut(s)	117, 1341
FbaI	TGATCA	2 cut(s)	757, 1837
FblI	GTMKAC	2 cut(s)	142, 2088
FokI	GGATG	5 cut(s)	121, 622, 1046, 1349, 1955
FspBI	CTAG	3 cut(s)	1050, 1110, 1767
GlaI	GCGC	3 cut(s)	254, 687, 706
HaeII	RGCGCY	1 cut(s)	689
HaeIII	GGCC	7 cut(s)	25, 131, 422, 1271, 1475, 1571, 1987
HapII	CCGG	5 cut(s)	233, 246, 298, 1476, 2110
HgaI	GACGC	2 cut(s)	114, 153
HhaI	GCGC	3 cut(s)	255, 688, 707
Hin6I	GCGC	3 cut(s)	253, 686, 705
HinP1I	GCGC	3 cut(s)	253, 686, 705
HincII	GTYRAC	2 cut(s)	143, 1906
HindII	GTYRAC	2 cut(s)	143, 1906
HindIII	AAGCTT	1 cut(s)	53
HpaII	CCGG	5 cut(s)	233, 246, 298, 1476, 2110
HphI	GGTGA	6 cut(s)	80, 364, 503, 1413, 1479, 1703
Hpy166II	GTNNAC	8 cut(s)	143, 195, 1723, 1741, 1795, 1906, 2020, 2089
Hpy188III	TCNNGA	7 cut(s)	833, 920, 941, 1129, 1601, 1841, 1895
Hpy8I	GTNNAC	8 cut(s)	143, 195, 1723, 1741, 1795, 1906, 2020, 2089
Hpy99I	CGWCG	8 cut(s)	147, 164, 209, 314, 317, 335, 364, 569
HpyAV	CCTTC	9 cut(s)	118, 400, 633, 788, 1136, 1648, 1682, 1933, 2041
HpyCH4III	ACNGT	5 cut(s)	457, 898, 1157, 1176, 1790
HpyCH4IV	ACGT	3 cut(s)	369, 567, 2085
HpyF3I	CTNAG	6 cut(s)	402, 435, 585, 615, 714, 841
HpySE526I	ACGT	3 cut(s)	369, 567, 2085
HspAI	GCGC	3 cut(s)	253, 686, 705
KpnI	GGTACC	1 cut(s)	2116
Ksp22I	TGATCA	2 cut(s)	757, 1837
Kzo9I	GATC	5 cut(s)	757, 780, 1042, 1443, 1837
LguI	GCTCTTC	1 cut(s)	906
LmnI	GCTCC	5 cut(s)	259, 379, 1009, 1630, 1924
Lsp1109I	GCAGC	8 cut(s)	120, 123, 285, 297, 300, 675, 1645, 1965
LweI	GCATC	8 cut(s)	838, 946, 1024, 1185, 1318, 1387, 1622, 1684
MaeI	CTAG	3 cut(s)	1050, 1110, 1767
MaeII	ACGT	3 cut(s)	369, 567, 2085
MaeIII	GTNAC	4 cut(s)	71, 370, 1080, 1188
MalI	GATC	5 cut(s)	759, 782, 1044, 1445, 1839
MbiI	CCGCTC	1 cut(s)	425
MboI	GATC	5 cut(s)	757, 780, 1042, 1443, 1837
MfeI	CAATTG	1 cut(s)	810
MflI	RGATCY	1 cut(s)	1443
MhlI	GDGCHC	1 cut(s)	655
MluCI	AATT	6 cut(s)	810, 1182, 1349, 1596, 1877, 2076
MmeI	TCCRAC	9 cut(s)	183, 192, 198, 204, 228, 237, 333, 1143, 1946
Mph1103I	ATGCAT	2 cut(s)	939, 1107
MroXI	GAANNNNTTC	2 cut(s)	915, 1781
MseI	TTAA	4 cut(s)	579, 630, 2055, 2094
MslI	CAYNNNNRTG	5 cut(s)	25, 517, 834, 932, 1506
MspI	CCGG	5 cut(s)	233, 246, 298, 1476, 2110
MunI	CAATTG	1 cut(s)	810
Mva1269I	GAATGC	2 cut(s)	1105, 2006
MvnI	CGCG	1 cut(s)	705
NcoI	CCATGG	2 cut(s)	1671, 1680
NdeII	GATC	5 cut(s)	757, 780, 1042, 1443, 1837
NlaIV	GGNNCC	6 cut(s)	302, 1068, 1530, 1678, 1920, 2114
NmeAIII	GCCGAG	2 cut(s)	415, 1102
NmuCI	GTSAC	2 cut(s)	370, 1188
NsiI	ATGCAT	2 cut(s)	939, 1107
NspI	RCATGY	1 cut(s)	1105
NspV	TTCGAA	1 cut(s)	479
PaeI	GCATGC	1 cut(s)	1105
PaeR7I	CTCGAG	1 cut(s)	426
PaqCI	CACCTGC	1 cut(s)	2109
PceI	AGGCCT	2 cut(s)	131, 1271
PciSI	GCTCTTC	1 cut(s)	906
PcsI	WCGNNNNNNNCGW	1 cut(s)	148
PctI	GAATGC	2 cut(s)	1105, 2006
PdmI	GAANNNNTTC	2 cut(s)	915, 1781
PfeI	GAWTC	7 cut(s)	67, 188, 209, 476, 481, 494, 1132
PflMI	CCANNNNNTGG	1 cut(s)	100
PinAI	ACCGGT	1 cut(s)	2109
Ppu21I	YACGTR	1 cut(s)	2086
PshAI	GACNNNNGTC	1 cut(s)	154
PsiI	TTATAA	1 cut(s)	1970
PspN4I	GGNNCC	6 cut(s)	302, 1068, 1530, 1678, 1920, 2114
PspPI	GGNCC	2 cut(s)	1528, 1985
PspXI	VCTCGAGB	1 cut(s)	426
PstNI	CAGNNNCTG	1 cut(s)	1430
PsuI	RGATCY	1 cut(s)	1443
RsaI	GTAC	7 cut(s)	194, 395, 819, 1207, 1760, 1820, 2114
RsaNI	GTAC	7 cut(s)	193, 394, 818, 1206, 1759, 1819, 2113
RseI	CAYNNNNRTG	5 cut(s)	25, 517, 834, 932, 1506
SalI	GTCGAC	1 cut(s)	141
SapI	GCTCTTC	1 cut(s)	906
SaqAI	TTAA	4 cut(s)	579, 630, 2055, 2094
Sau3AI	GATC	5 cut(s)	757, 780, 1042, 1443, 1837
Sau96I	GGNCC	2 cut(s)	1528, 1985
ScaI	AGTACT	1 cut(s)	395
SduI	GDGCHC	1 cut(s)	655
SfaNI	GCATC	8 cut(s)	838, 946, 1024, 1185, 1318, 1387, 1622, 1684
SfcI	CTRYAG	1 cut(s)	1431
Sfr274I	CTCGAG	1 cut(s)	426
SfuI	TTCGAA	1 cut(s)	479
SinI	GGWCC	1 cut(s)	1528
SlaI	CTCGAG	1 cut(s)	426
SmiMI	CAYNNNNRTG	5 cut(s)	25, 517, 834, 932, 1506
SmlI	CTYRAG	2 cut(s)	426, 1322
SmoI	CTYRAG	2 cut(s)	426, 1322
SphI	GCATGC	1 cut(s)	1105
Sse9I	AATT	6 cut(s)	810, 1182, 1349, 1596, 1877, 2076
SseBI	AGGCCT	2 cut(s)	131, 1271
SsiI	CCGC	6 cut(s)	91, 292, 295, 343, 423, 2116
SspI	AATATT	2 cut(s)	1701, 1781
SspMI	CTAG	3 cut(s)	1050, 1110, 1767
StuI	AGGCCT	2 cut(s)	131, 1271
StyI	CCWWGG	4 cut(s)	94, 1212, 1671, 1680
TaaI	ACNGT	5 cut(s)	457, 898, 1157, 1176, 1790
TaiI	ACGT	3 cut(s)	372, 570, 2088
TasI	AATT	6 cut(s)	810, 1182, 1349, 1596, 1877, 2076
TatI	WGTACW	3 cut(s)	393, 817, 1818
TauI	GCSGC	3 cut(s)	294, 297, 425
TfiI	GAWTC	7 cut(s)	67, 188, 209, 476, 481, 494, 1132
Tru1I	TTAA	4 cut(s)	579, 630, 2055, 2094
Tru9I	TTAA	4 cut(s)	579, 630, 2055, 2094
TseFI	GTSAC	2 cut(s)	370, 1188
TseI	GCWGC	8 cut(s)	108, 111, 273, 285, 288, 688, 1658, 1953
Tsp45I	GTSAC	2 cut(s)	370, 1188
TspDTI	ATGAA	8 cut(s)	339, 1356, 1470, 1524, 1667, 1679, 1821, 1967
TspGWI	ACGGA	1 cut(s)	2000
Van91I	CCANNNNNTGG	1 cut(s)	100
VpaK11BI	GGWCC	1 cut(s)	1528
XapI	RAATTY	3 cut(s)	1596, 1877, 2076
XceI	RCATGY	1 cut(s)	1105
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1669, 1678
XhoI	CTCGAG	1 cut(s)	426
XmiI	GTMKAC	2 cut(s)	142, 2088
XmnI	GAANNNNTTC	2 cut(s)	915, 1781
XspI	CTAG	3 cut(s)	1050, 1110, 1767
ZrmI	AGTACT	1 cut(s)	395
Zsp2I	ATGCAT	2 cut(s)	939, 1107

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.