Rorug05G0031500

WPP domain-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001383
Physical Location & Seq
Reverse (-)
2022593 .. 2024293
1701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0031500.1

Sequence Viewer

Length: 1701 bp
ATGCTTCTTCTTCGCAAAGCTTATTCAACAACTTCCAGTACTTCCCTAACTTGGAGAACCCAAATCAAACAAGCTCACTTAGCCTCCCAAATCTCCTCGATTCTCTTACAGAGACGCAACTGGGTTCCACTCCTCCAAAACCTCAACGTCCCCAAACTAAACCCTCCTCTGTTTCTCCAGATCATCCACAAAATCCAGAGCAACCCACAACTCTCTTTGGATTTCTTCAACTGGGCCAAGTCCAATTTACGTTTCGAACCAGACCTCAAATCCCACTGCCAAATCATCCAACTCTCACTCGGGTCTGGCCTCACCCGACCCGCCAAACCCATCTTGGACACACTAATCCAGACCCATCATGTTTCCAAGCTTGTACAGTCCATGTCTCAGGCTTGTAAAGGTACAGATTCTCTATCCAATATGTTAAGTTTTGTGCTTGGTTGCTATGCAAGAAAGGGTTTGGTTAGAGAAGGCATAGAAGTTTATAGAGAAATGAGTGTTCTTGGTTGTGTGCCTTCAGTTTTAGCCTGCAATGCATTGCTTAATGCTATGAGGCAAGGAAATGAGACTAGATTGGGTTGGTGTTTTTATGGGGTTATGCTCAGAAATGGGGTTTCACCAGACCAGTTTACATGGTCTCTGGTTGCTCAGTTTCTTTGTAAAGATGGGAAATTTGAAAGGATTCTTAGACTTTTGGATTTGGGTGTTTGTGATTCTATGTTTTATAGTCTTCTTGTCGAAGGTTATAGCAAAAGTGGGAATTTTGATGCTGCATTTTGCTACTTGGAGAAAATGAGTGATGGGAAATTGGAACCTGGTTTTAGTACTTATAGTTCTGTTCTTGATGGGGCATGTAAACTTGGGTATGTGGAAGTGGTTGAAAGGGTAATGAGGATTATGGTAGAGAAGAAATTGCTTTCGGAGAGTCCTGTTTCGGAGTATGATTCAATTGTTAAGAAGCTTTGTGCTTTGGACAAAACTTATGCGGCAGAGATGTTCTTTAAGAAAGCGTGTGATGAGAAAATTGAATTGCAAGGTGCTACTTATGGATGTATGCTGCAGGCATTGACTAAAGAAATGAGAATAGTGGAAGCTATTCGGGTTTATCGGGTGGTTTGTGAGAGGGGTATAGTAGTGGATGATAGCAGTTATTATGCATTTGCTAATGTCCTTTGCAAGGAAGAGCGATCTGAGGAGGGTTTCGAGTTGTTAATGGATGTTATAAGAAGAGGGTTTAGTCCTTGTGCATCAGAATTGTCTGGATTTATATCATTTCTATGTGGCAGGCGTAGATGGAGAGAAGCAGAAGATGTGTTGAATTCAGTTCTAGACAAAGGGCTACTACCGGATTTGACTTCTTGTTCTTCGTTAGTAGGGCATTACTGCTCTAGAAGACGGATTGATTCAGCGATGGCATTGCACAGTAAGATGGAAAAACTGAACGGCAGTTTGGATGTGACAACTTACAATGTACTTCTTGGTGGGCTTTTTGCAGCAAGGAGAGTTGAAGAAGCAGTGAGAGTGTTTGATTACATGAGAATTCACAACTTGATGAGCAGCGAAAGCTTTACGATCATGATTCGTGGGCTTTGTCAAGTAAAGGAACTGAGAAAGGCCATGAAAATTCATGATGAAATGTTGAAGATGGGACTTAAGCCTCCTGCAGCAACTTATAAACGTTTGATATATGCATTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
No domains found.

Protein Analysis

566

Amino Acids

64.03

Weight (kDa)

8.95

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 172 - 221 4.3e-06 PPR repeat family
PPR_2 PF13041 239 - 286 3.6e-07 PPR repeat family
PPR_2 PF13041 449 - 496 2.1e-08 PPR repeat family
PPR_1 PF12854 484 - 513 4.3e-06 PPR repeat
PPR_2 PF13041 485 - 532 1.2e-10 PPR repeat family
PPR_2 PF13041 521 - 565 1.2e-08 PPR repeat family
PPR PF01535 522 - 551 3.2e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1223, 1674
AciI CCGC 2 cut(s) 321, 986
AclI AACGTT 1 cut(s) 1678
AcsI RAATTY 5 cut(s) 671, 760, 1318, 1539, 1623
AcuI CTGAAG 1 cut(s) 501
AfaI GTAC 5 cut(s) 40, 375, 403, 826, 1473
AfiI CCNNNNNNNGG 2 cut(s) 51, 1177
AflII CTTAAG 1 cut(s) 1652
AjnI CCWGG 1 cut(s) 814
AjuI GAANNNNNNNTTGG 4 cut(s) 236, 268, 1433, 1465
AluBI AGCT 6 cut(s) 20, 74, 370, 961, 1094, 1566
AluI AGCT 6 cut(s) 20, 74, 370, 961, 1094, 1566
Alw26I GTCTC 4 cut(s) 106, 390, 560, 642
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 3 cut(s) 234, 307, 1614
ApeKI GCWGC 5 cut(s) 770, 1057, 1493, 1557, 1664
ApoI RAATTY 5 cut(s) 671, 760, 1318, 1539, 1623
Asp700I GAANNNNTTC 2 cut(s) 681, 1095
AspS9I GGNCC 1 cut(s) 234
AsuHPI GGTGA 2 cut(s) 304, 609
AsuII TTCGAA 1 cut(s) 255
AvaI CYCGRG 1 cut(s) 299
BbsI GAAGAC 2 cut(s) 722, 1399
BbvI GCAGC 5 cut(s) 757, 1044, 1505, 1569, 1676
BccI CCATC 9 cut(s) 338, 363, 659, 794, 839, 1287, 1405, 1423, 1639
BceAI ACGGC 1 cut(s) 1459
BcgI CGANNNNNNTGC 2 cut(s) 1399, 1433
BciT130I CCWGG 1 cut(s) 816
BcoDI GTCTC 4 cut(s) 106, 390, 560, 642
BfaI CTAG 3 cut(s) 570, 1328, 1389
BfmI CTRYAG 2 cut(s) 1058, 1662
BfrI CTTAAG 1 cut(s) 1652
BisI GCNGC 6 cut(s) 771, 987, 1058, 1494, 1558, 1665
BlsI GCNGC 6 cut(s) 772, 988, 1059, 1495, 1559, 1666
BmcAI AGTACT 2 cut(s) 40, 826
Bme1390I CCNGG 1 cut(s) 816
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 1 cut(s) 234
BmiI GGNNCC 2 cut(s) 126, 813
BmrFI CCNGG 1 cut(s) 816
BmrI ACTGGG 2 cut(s) 130, 241
BmsI GCATC 2 cut(s) 757, 1256
BmuI ACTGGG 2 cut(s) 130, 241
BpiI GAAGAC 2 cut(s) 722, 1399
BpmI CTGGAG 1 cut(s) 161
Bpu14I TTCGAA 1 cut(s) 255
BsaBI GATNNNNATC 1 cut(s) 1267
BsaI GGTCTC 1 cut(s) 642
BsaWI WCCGGW 1 cut(s) 1345
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 1177
Bse1I ACTGG 4 cut(s) 36, 125, 236, 625
Bse3DI GCAATG 3 cut(s) 536, 538, 1415
Bse8I GATNNNNATC 1 cut(s) 1267
BseBI CCWGG 1 cut(s) 816
BseGI GGATG 6 cut(s) 183, 285, 1055, 1144, 1222, 1459
BseJI GATNNNNATC 1 cut(s) 1267
BseLI CCNNNNNNNGG 2 cut(s) 51, 1177
BseMI GCAATG 3 cut(s) 536, 538, 1415
BseMII CTCAG 5 cut(s) 401, 616, 662, 1182, 1598
BseNI ACTGG 4 cut(s) 36, 125, 236, 625
BseRI GAGGAG 4 cut(s) 85, 122, 156, 1208
BseXI GCAGC 5 cut(s) 757, 1044, 1505, 1569, 1676
BshFI GGCC 3 cut(s) 236, 309, 1616
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 1 cut(s) 1346
BslFI GGGAC 2 cut(s) 134, 1662
BslI CCNNNNNNNGG 2 cut(s) 51, 1177
BsmAI GTCTC 4 cut(s) 106, 390, 560, 642
BsmBI CGTCTC 1 cut(s) 106
BsmFI GGGAC 2 cut(s) 134, 1662
BsmI GAATGC 1 cut(s) 1691
BsnI GGCC 3 cut(s) 236, 309, 1616
Bso31I GGTCTC 1 cut(s) 642
BsoBI CYCGRG 1 cut(s) 299
Bsp119I TTCGAA 1 cut(s) 255
Bsp1407I TGTACA 1 cut(s) 373
Bsp143I GATC 3 cut(s) 180, 1187, 1572
BspACI CCGC 2 cut(s) 321, 986
BspANI GGCC 3 cut(s) 236, 309, 1616
BspCNI CTCAG 5 cut(s) 400, 615, 661, 1183, 1599
BspHI TCATGA 2 cut(s) 1575, 1627
BspLI GGNNCC 2 cut(s) 126, 813
BspMAI CTGCAG 2 cut(s) 1062, 1666
BspQI GCTCTTC 1 cut(s) 1176
BspT104I TTCGAA 1 cut(s) 255
BspTI CTTAAG 1 cut(s) 1652
BspTNI GGTCTC 1 cut(s) 642
BsrDI GCAATG 3 cut(s) 536, 538, 1415
BsrGI TGTACA 1 cut(s) 373
BsrI ACTGG 4 cut(s) 36, 125, 236, 625
BssMI GATC 3 cut(s) 180, 1187, 1572
Bst2UI CCWGG 1 cut(s) 816
Bst4CI ACNGT 2 cut(s) 378, 1424
Bst6I CTCTTC 2 cut(s) 1176, 1222
BstAFI CTTAAG 1 cut(s) 1652
BstAUI TGTACA 1 cut(s) 373
BstBI TTCGAA 1 cut(s) 255
BstC8I GCNNGC 3 cut(s) 529, 1062, 1286
BstDEI CTNAG 7 cut(s) 79, 387, 602, 648, 686, 1191, 1607
BstENI CCTNNNNNAGG 1 cut(s) 1175
BstF5I GGATG 6 cut(s) 183, 285, 1055, 1144, 1222, 1459
BstKTI GATC 3 cut(s) 183, 1190, 1575
BstMAI GTCTC 4 cut(s) 106, 390, 560, 642
BstMBI GATC 3 cut(s) 180, 1187, 1572
BstMWI GCNNNNNNNGC 3 cut(s) 80, 533, 1563
BstNI CCWGG 1 cut(s) 816
BstNSI RCATGY 1 cut(s) 855
BstSCI CCNGG 1 cut(s) 814
BstSFI CTRYAG 2 cut(s) 1058, 1662
BstV1I GCAGC 5 cut(s) 757, 1044, 1505, 1569, 1676
BstV2I GAAGAC 2 cut(s) 722, 1399
BsuRI GGCC 3 cut(s) 236, 309, 1616
BtgZI GCGATG 1 cut(s) 1424
BtsCI GGATG 6 cut(s) 183, 285, 1055, 1144, 1222, 1459
BtsI GCAGTG 2 cut(s) 274, 1521
BtsIMutI CAGTG 2 cut(s) 274, 1521
Cac8I GCNNGC 3 cut(s) 529, 1062, 1286
CciI TCATGA 2 cut(s) 1575, 1627
Cfr13I GGNCC 1 cut(s) 234
CseI GACGC 1 cut(s) 123
CsiI ACCWGGT 1 cut(s) 814
Csp6I GTAC 5 cut(s) 39, 374, 402, 825, 1472
CviAII CATG 8 cut(s) 359, 382, 633, 852, 1534, 1576, 1618, 1628
CviQI GTAC 5 cut(s) 39, 374, 402, 825, 1472
DdeI CTNAG 7 cut(s) 79, 387, 602, 648, 686, 1191, 1607
DpnI GATC 3 cut(s) 182, 1189, 1574
DpnII GATC 3 cut(s) 180, 1187, 1572
Eam1104I CTCTTC 2 cut(s) 1176, 1222
EarI CTCTTC 2 cut(s) 1176, 1222
Eco31I GGTCTC 1 cut(s) 642
Eco57I CTGAAG 1 cut(s) 501
Eco88I CYCGRG 1 cut(s) 299
EcoNI CCTNNNNNAGG 1 cut(s) 1175
EcoRI GAATTC 2 cut(s) 1318, 1539
EcoRII CCWGG 1 cut(s) 814
EcoT22I ATGCAT 3 cut(s) 538, 1159, 1693
Esp3I CGTCTC 1 cut(s) 106
FaeI CATG 8 cut(s) 362, 385, 636, 855, 1537, 1579, 1621, 1631
FaqI GGGAC 2 cut(s) 134, 1662
FatI CATG 8 cut(s) 358, 381, 632, 851, 1533, 1575, 1617, 1627
FauI CCCGC 1 cut(s) 328
Fnu4HI GCNGC 6 cut(s) 771, 987, 1058, 1494, 1558, 1665
FokI GGATG 6 cut(s) 170, 272, 1062, 1151, 1229, 1466
Fsp4HI GCNGC 6 cut(s) 771, 987, 1058, 1494, 1558, 1665
FspBI CTAG 3 cut(s) 570, 1328, 1389
GluI GCNGC 6 cut(s) 771, 987, 1058, 1494, 1558, 1665
GsuI CTGGAG 1 cut(s) 161
HaeIII GGCC 3 cut(s) 236, 309, 1616
HapII CCGG 1 cut(s) 1346
HgaI GACGC 1 cut(s) 123
Hin1II CATG 8 cut(s) 362, 385, 636, 855, 1537, 1579, 1621, 1631
HindIII AAGCTT 4 cut(s) 18, 368, 959, 1564
HinfI GANTC 8 cut(s) 100, 407, 682, 713, 925, 944, 1403, 1579
HpaII CCGG 1 cut(s) 1346
HphI GGTGA 2 cut(s) 304, 609
Hpy166II GTNNAC 2 cut(s) 630, 857
Hpy188I TCNGA 5 cut(s) 605, 922, 937, 1192, 1252
Hpy188III TCNNGA 9 cut(s) 178, 196, 349, 842, 1260, 1328, 1389, 1576, 1628
Hpy8I GTNNAC 2 cut(s) 630, 857
HpyAV CCTTC 3 cut(s) 464, 525, 734
HpyCH4III ACNGT 2 cut(s) 378, 1424
HpyCH4IV ACGT 3 cut(s) 147, 250, 1678
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 533, 1563
HpyF3I CTNAG 7 cut(s) 79, 387, 602, 648, 686, 1191, 1607
HpySE526I ACGT 3 cut(s) 147, 250, 1678
Hsp92II CATG 8 cut(s) 362, 385, 636, 855, 1537, 1579, 1621, 1631
Kzo9I GATC 3 cut(s) 180, 1187, 1572
LguI GCTCTTC 1 cut(s) 1176
Lsp1109I GCAGC 5 cut(s) 757, 1044, 1505, 1569, 1676
LweI GCATC 2 cut(s) 757, 1256
MabI ACCWGGT 1 cut(s) 814
MaeI CTAG 3 cut(s) 570, 1328, 1389
MaeII ACGT 3 cut(s) 147, 250, 1678
MaeIII GTNAC 1 cut(s) 1456
MalI GATC 3 cut(s) 182, 1189, 1574
MboI GATC 3 cut(s) 180, 1187, 1572
MfeI CAATTG 1 cut(s) 948
MlyI GAGTC 1 cut(s) 934
MmeI TCCRAC 1 cut(s) 313
Mph1103I ATGCAT 3 cut(s) 538, 1159, 1693
MroXI GAANNNNTTC 2 cut(s) 681, 1095
MseI TTAA 6 cut(s) 425, 543, 954, 1002, 1211, 1653
MslI CAYNNNNRTG 1 cut(s) 1276
MspCI CTTAAG 1 cut(s) 1652
MspI CCGG 1 cut(s) 1346
MspR9I CCNGG 1 cut(s) 816
MunI CAATTG 1 cut(s) 948
Mva1269I GAATGC 1 cut(s) 1691
MvaI CCWGG 1 cut(s) 816
MwoI GCNNNNNNNGC 3 cut(s) 80, 533, 1563
NdeII GATC 3 cut(s) 180, 1187, 1572
NlaIII CATG 8 cut(s) 362, 385, 636, 855, 1537, 1579, 1621, 1631
NlaIV GGNNCC 2 cut(s) 126, 813
NmuCI GTSAC 1 cut(s) 1456
NsiI ATGCAT 3 cut(s) 538, 1159, 1693
NspI RCATGY 1 cut(s) 855
NspV TTCGAA 1 cut(s) 255
PagI TCATGA 2 cut(s) 1575, 1627
PciSI GCTCTTC 1 cut(s) 1176
PctI GAATGC 1 cut(s) 1691
PdmI GAANNNNTTC 2 cut(s) 681, 1095
PfeI GAWTC 7 cut(s) 100, 407, 682, 713, 944, 1403, 1579
PkrI GCNGC 6 cut(s) 772, 988, 1059, 1495, 1559, 1666
PleI GAGTC 1 cut(s) 933
PpsI GAGTC 1 cut(s) 933
PsiI TTATAA 2 cut(s) 1223, 1674
Psp1406I AACGTT 1 cut(s) 1678
Psp6I CCWGG 1 cut(s) 814
PspGI CCWGG 1 cut(s) 814
PspN4I GGNNCC 2 cut(s) 126, 813
PspPI GGNCC 1 cut(s) 234
PsrI GAACNNNNNNTAC 2 cut(s) 817, 849
PstI CTGCAG 2 cut(s) 1062, 1666
RsaI GTAC 5 cut(s) 40, 375, 403, 826, 1473
RsaNI GTAC 5 cut(s) 39, 374, 402, 825, 1472
RseI CAYNNNNRTG 1 cut(s) 1276
SapI GCTCTTC 1 cut(s) 1176
SaqAI TTAA 6 cut(s) 425, 543, 954, 1002, 1211, 1653
SatI GCNGC 6 cut(s) 771, 987, 1058, 1494, 1558, 1665
Sau3AI GATC 3 cut(s) 180, 1187, 1572
Sau96I GGNCC 1 cut(s) 234
ScaI AGTACT 2 cut(s) 40, 826
SchI GAGTC 1 cut(s) 934
ScrFI CCNGG 1 cut(s) 816
SexAI ACCWGGT 1 cut(s) 814
SfaNI GCATC 2 cut(s) 757, 1256
SfcI CTRYAG 2 cut(s) 1058, 1662
SfuI TTCGAA 1 cut(s) 255
SmiMI CAYNNNNRTG 1 cut(s) 1276
SmlI CTYRAG 1 cut(s) 1652
SmoI CTYRAG 1 cut(s) 1652
SsiI CCGC 2 cut(s) 321, 986
SspMI CTAG 3 cut(s) 570, 1328, 1389
StyD4I CCNGG 1 cut(s) 814
TaaI ACNGT 2 cut(s) 378, 1424
TaiI ACGT 3 cut(s) 150, 253, 1681
TaqI TCGA 4 cut(s) 98, 255, 738, 1203
TatI WGTACW 4 cut(s) 38, 373, 824, 1471
TauI GCSGC 1 cut(s) 989
TfiI GAWTC 7 cut(s) 100, 407, 682, 713, 944, 1403, 1579
Tru1I TTAA 6 cut(s) 425, 543, 954, 1002, 1211, 1653
Tru9I TTAA 6 cut(s) 425, 543, 954, 1002, 1211, 1653
TscAI CASTG 2 cut(s) 281, 1521
TseFI GTSAC 1 cut(s) 1456
TseI GCWGC 5 cut(s) 770, 1057, 1493, 1557, 1664
Tsp45I GTSAC 1 cut(s) 1456
TspDTI ATGAA 3 cut(s) 1616, 1634, 1647
TspGWI ACGGA 1 cut(s) 1411
TspRI CASTG 2 cut(s) 281, 1521
Vha464I CTTAAG 1 cut(s) 1652
XagI CCTNNNNNAGG 1 cut(s) 1175
XapI RAATTY 5 cut(s) 671, 760, 1318, 1539, 1623
XbaI TCTAGA 2 cut(s) 1327, 1388
XceI RCATGY 1 cut(s) 855
XcmI CCANNNNNNNNNTGG 1 cut(s) 331
XmnI GAANNNNTTC 2 cut(s) 681, 1095
XspI CTAG 3 cut(s) 570, 1328, 1389
ZrmI AGTACT 2 cut(s) 40, 826
Zsp2I ATGCAT 3 cut(s) 538, 1159, 1693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.