Rorug05G0342100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004205
Physical Location & Seq
Forward (+)
41832157 .. 41833079
923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0342100.1

Sequence Viewer

Length: 426 bp
ATGGGAGCGGAGAAAGCAGAGGCGTCGTTAGATTCGCTGATCGCTGGGTTCAACAGTCGGATCTCAGAGCTCCAAGAGCTCGTTATTGCGCGAAAAACCAGCAGTGTCACCGATTTGTCGGCCGTCGACGCCGCCATGAAGGACATGGAGCTTCAGGTCCAGGCCATCAAGGACCGGGTTCGTGACGAAACCCTAGCCATCCCCAAAGCAAAAGTAGTTCCCTCTCTCTTTCTCTCTAGGTTTTGCTCTTCACTGCCACTACCAGCTTCTGTCGCTGAACCTCCAAACGACTCCATTTCGAGCCTCAGCTTCAGCCCCAAAGCCAATCACCTGTCGCCACCTCCTGGGACAACTAGGTTTGATGCTGGGAGATACAACACAGTGGACAAAATCTTGGTAGTATCCCAAGGGAATGATTTCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

15.11

Weight (kDa)

5.37

Isoelectric Point (pI)

39.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKA1 PF07160 9 - 74 4.8e-12 Spindle and kinetochore-associated protein 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 344
AccBSI CCGCTC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 126
AccII CGCG 1 cut(s) 91
AciI CCGC 2 cut(s) 8, 132
AclWI GGATC 1 cut(s) 68
AcoI YGGCCR 1 cut(s) 120
AcuI CTGAAG 2 cut(s) 137, 295
AcyI GRCGYC 2 cut(s) 23, 129
AfiI CCNNNNNNNGG 1 cut(s) 344
AgsI TTSAA 1 cut(s) 52
AjnI CCWGG 2 cut(s) 159, 343
AluBI AGCT 5 cut(s) 70, 79, 151, 266, 309
AluI AGCT 5 cut(s) 70, 79, 151, 266, 309
Alw21I GWGCWC 2 cut(s) 72, 81
AlwI GGATC 1 cut(s) 68
AlwNI CAGNNNCTG 1 cut(s) 269
AoxI GGCC 2 cut(s) 120, 162
Asp700I GAANNNNTTC 1 cut(s) 416
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 2 cut(s) 157, 172
AsuC2I CCSGG 1 cut(s) 176
AsuHPI GGTGA 2 cut(s) 100, 320
AvaII GGWCC 2 cut(s) 157, 172
BanII GRGCYC 2 cut(s) 72, 81
Bbv12I GWGCWC 2 cut(s) 72, 81
BbvCI CCTCAGC 1 cut(s) 305
BccI CCATC 2 cut(s) 173, 206
BceAI ACGGC 1 cut(s) 107
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 2 cut(s) 161, 345
BciVI GTATCC 1 cut(s) 412
BcnI CCSGG 1 cut(s) 176
BfaI CTAG 3 cut(s) 194, 237, 354
BfuI GTATCC 1 cut(s) 412
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme1390I CCNGG 3 cut(s) 161, 176, 345
Bme18I GGWCC 2 cut(s) 157, 172
BmgT120I GGNCC 2 cut(s) 157, 172
BmrFI CCNGG 3 cut(s) 161, 176, 345
BmsI GCATC 1 cut(s) 352
Bpu10I CCTNAGC 1 cut(s) 305
BpuMI CCSGG 1 cut(s) 176
BsaHI GRCGYC 2 cut(s) 23, 129
BsaJI CCNNGG 2 cut(s) 344, 406
Bsc4I CCNNNNNNNGG 1 cut(s) 344
BseBI CCWGG 2 cut(s) 161, 345
BseDI CCNNGG 2 cut(s) 344, 406
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 1 cut(s) 344
BseMII CTCAG 2 cut(s) 78, 319
BseX3I CGGCCG 1 cut(s) 120
BseYI CCCAGC 2 cut(s) 44, 365
Bsh1236I CGCG 1 cut(s) 91
Bsh1285I CGRYCG 1 cut(s) 123
BshFI GGCC 2 cut(s) 122, 164
BsiEI CGRYCG 1 cut(s) 123
BsiHKAI GWGCWC 2 cut(s) 72, 81
BsiSI CCGG 1 cut(s) 175
BslFI GGGAC 1 cut(s) 361
BslI CCNNNNNNNGG 1 cut(s) 344
BsmFI GGGAC 1 cut(s) 361
BsnI GGCC 2 cut(s) 122, 164
Bsp1286I GDGCHC 2 cut(s) 72, 81
Bsp143I GATC 2 cut(s) 39, 60
BspACI CCGC 2 cut(s) 8, 132
BspANI GGCC 2 cut(s) 122, 164
BspCNI CTCAG 2 cut(s) 77, 318
BspFNI CGCG 1 cut(s) 91
BspPI GGATC 1 cut(s) 68
BspQI GCTCTTC 1 cut(s) 253
BsrBI CCGCTC 1 cut(s) 8
BssECI CCNNGG 2 cut(s) 344, 406
BssMI GATC 2 cut(s) 39, 60
BssNI GRCGYC 2 cut(s) 23, 129
BssT1I CCWWGG 1 cut(s) 406
Bst2UI CCWGG 2 cut(s) 161, 345
Bst4CI ACNGT 2 cut(s) 56, 382
Bst6I CTCTTC 1 cut(s) 253
BstACI GRCGYC 2 cut(s) 23, 129
BstDEI CTNAG 2 cut(s) 64, 305
BstF5I GGATG 1 cut(s) 198
BstFNI CGCG 1 cut(s) 91
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 2 cut(s) 42, 63
BstMBI GATC 2 cut(s) 39, 60
BstMCI CGRYCG 1 cut(s) 123
BstMWI GCNNNNNNNGC 4 cut(s) 14, 76, 128, 272
BstNI CCWGG 2 cut(s) 161, 345
BstSCI CCNGG 3 cut(s) 159, 174, 343
BstUI CGCG 1 cut(s) 91
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
BstZI CGGCCG 1 cut(s) 120
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 2 cut(s) 122, 164
BtsCI GGATG 1 cut(s) 198
BtsI GCAGTG 2 cut(s) 109, 251
BtsIMutI CAGTG 3 cut(s) 109, 251, 387
CaiI CAGNNNCTG 1 cut(s) 269
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 2 cut(s) 157, 172
CseI GACGC 2 cut(s) 12, 137
CviAII CATG 3 cut(s) 136, 145, 423
DdeI CTNAG 2 cut(s) 64, 305
DpnI GATC 2 cut(s) 41, 62
DpnII GATC 2 cut(s) 39, 60
EaeI YGGCCR 1 cut(s) 120
EagI CGGCCG 1 cut(s) 120
Eam1104I CTCTTC 1 cut(s) 253
EarI CTCTTC 1 cut(s) 253
Ecl136II GAGCTC 2 cut(s) 70, 79
EclXI CGGCCG 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 406
Eco24I GRGCYC 2 cut(s) 72, 81
Eco47I GGWCC 2 cut(s) 157, 172
Eco52I CGGCCG 1 cut(s) 120
Eco53kI GAGCTC 2 cut(s) 70, 79
Eco57I CTGAAG 2 cut(s) 137, 295
EcoICRI GAGCTC 2 cut(s) 70, 79
EcoRII CCWGG 2 cut(s) 159, 343
EcoT14I CCWWGG 1 cut(s) 406
EcoT38I GRGCYC 2 cut(s) 72, 81
ErhI CCWWGG 1 cut(s) 406
FaeI CATG 3 cut(s) 139, 148, 426
FaiI YATR 3 cut(s) 137, 146, 424
FaqI GGGAC 1 cut(s) 361
FatI CATG 3 cut(s) 135, 144, 422
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 1 cut(s) 185
FriOI GRGCYC 2 cut(s) 72, 81
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 3 cut(s) 194, 237, 354
GlaI GCGC 1 cut(s) 90
GluI GCNGC 1 cut(s) 132
GsaI CCCAGC 2 cut(s) 48, 369
HaeIII GGCC 2 cut(s) 122, 164
HapII CCGG 1 cut(s) 175
HgaI GACGC 2 cut(s) 12, 137
HhaI GCGC 1 cut(s) 91
Hin1I GRCGYC 2 cut(s) 23, 129
Hin1II CATG 3 cut(s) 139, 148, 426
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 2 cut(s) 32, 290
HpaII CCGG 1 cut(s) 175
HphI GGTGA 2 cut(s) 100, 320
Hpy166II GTNNAC 2 cut(s) 127, 385
Hpy188I TCNGA 2 cut(s) 60, 67
Hpy188III TCNNGA 1 cut(s) 182
Hpy8I GTNNAC 2 cut(s) 127, 385
Hpy99I CGWCG 3 cut(s) 28, 128, 131
HpyAV CCTTC 1 cut(s) 133
HpyCH4III ACNGT 2 cut(s) 56, 382
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 76, 128, 272
HpyF3I CTNAG 2 cut(s) 64, 305
Hsp92I GRCGYC 2 cut(s) 23, 129
Hsp92II CATG 3 cut(s) 139, 148, 426
HspAI GCGC 1 cut(s) 89
Kzo9I GATC 2 cut(s) 39, 60
LguI GCTCTTC 1 cut(s) 253
LmnI GCTCC 3 cut(s) 5, 75, 148
LweI GCATC 1 cut(s) 352
MaeI CTAG 3 cut(s) 194, 237, 354
MaeIII GTNAC 2 cut(s) 106, 182
MalI GATC 2 cut(s) 41, 62
MbiI CCGCTC 1 cut(s) 8
MboI GATC 2 cut(s) 39, 60
MboII GAAGA 1 cut(s) 240
MflI RGATCY 1 cut(s) 60
MhlI GDGCHC 2 cut(s) 72, 81
MlyI GAGTC 1 cut(s) 284
MmeI TCCRAC 1 cut(s) 38
MnlI CCTC 5 cut(s) 13, 232, 291, 314, 351
MroXI GAANNNNTTC 1 cut(s) 416
MspI CCGG 1 cut(s) 175
MspR9I CCNGG 3 cut(s) 161, 176, 345
MvaI CCWGG 2 cut(s) 161, 345
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 4 cut(s) 14, 76, 128, 272
NciI CCSGG 1 cut(s) 176
NdeII GATC 2 cut(s) 39, 60
NlaIII CATG 3 cut(s) 139, 148, 426
NmuCI GTSAC 2 cut(s) 106, 182
PciSI GCTCTTC 1 cut(s) 253
PdmI GAANNNNTTC 1 cut(s) 416
PfeI GAWTC 1 cut(s) 32
PflMI CCANNNNNTGG 1 cut(s) 344
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
Psp124BI GAGCTC 2 cut(s) 72, 81
Psp6I CCWGG 2 cut(s) 159, 343
PspFI CCCAGC 2 cut(s) 44, 365
PspGI CCWGG 2 cut(s) 159, 343
PspPI GGNCC 2 cut(s) 157, 172
PstNI CAGNNNCTG 1 cut(s) 269
PsuI RGATCY 1 cut(s) 60
SacI GAGCTC 2 cut(s) 72, 81
SalI GTCGAC 1 cut(s) 125
SapI GCTCTTC 1 cut(s) 253
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 2 cut(s) 39, 60
Sau96I GGNCC 2 cut(s) 157, 172
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 3 cut(s) 161, 176, 345
SduI GDGCHC 2 cut(s) 72, 81
SfaNI GCATC 1 cut(s) 352
SgrDI CGTCGACG 1 cut(s) 125
SinI GGWCC 2 cut(s) 157, 172
SsiI CCGC 2 cut(s) 8, 132
SspMI CTAG 3 cut(s) 194, 237, 354
SstI GAGCTC 2 cut(s) 72, 81
StyD4I CCNGG 3 cut(s) 159, 174, 343
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 2 cut(s) 56, 382
TaqI TCGA 2 cut(s) 126, 299
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 1 cut(s) 32
TscAI CASTG 3 cut(s) 109, 258, 387
TseFI GTSAC 2 cut(s) 106, 182
Tsp45I GTSAC 2 cut(s) 106, 182
TspDTI ATGAA 1 cut(s) 152
TspRI CASTG 3 cut(s) 109, 258, 387
Van91I CCANNNNNTGG 1 cut(s) 344
VpaK11BI GGWCC 2 cut(s) 157, 172
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
XmiI GTMKAC 1 cut(s) 126
XmnI GAANNNNTTC 1 cut(s) 416
XspI CTAG 3 cut(s) 194, 237, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.