Rh5AG404000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004205
Physical Location & Seq
Forward (+)
70002335 .. 70009755
7421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG404000.1

Sequence Viewer

Length: 507 bp
ATGTGGCTTGGGTCAAACCCAGACGCCTCATCCACGATAACAAATGGGGGAGGTATCTTAAGACATGCCACATCCAATCTTGCAAGCGGACGCCCATTTAAGCACAAGTGCTTGCCAATTCCGGGCATTGTTCACTCACCCGTACTGGACATTCCAACTGTAACTTCTAGCAGAAGAGAATACAGCAAGCTTCTGATTTGCTTTGATATAATCAGTGAAGTATGTGTGTTTTCATATGAAAGCAGAGGTGAAACTAGGAATAATAACAGGTGGGTCGGGGATAAGATTACAGTACCAGGCTCGAAGAAGATGGAAATCCCAGAATCCAACTCCAACCACTGCCTAAAACGCCTCCCTCCTCCTAGAGGGCAGATCAAGAAAAAGATCTTCAAATCAATAACTTCAGCGGTGGGTGGGTTCATTCGCAAGCCGAAAGAAGATGGATCCCAAATTCCAGCTGAACCGCGGAGCGGGTACTCCTCAGATGCTCAATCCGAAAGTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

168

Amino Acids

18.37

Weight (kDa)

9.62

Isoelectric Point (pI)

52.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 471
AccII CGCG 1 cut(s) 466
AciI CCGC 5 cut(s) 87, 407, 464, 466, 471
AclWI GGATC 2 cut(s) 438, 451
AcsI RAATTY 1 cut(s) 450
AcuI CTGAAG 1 cut(s) 387
AcyI GRCGYC 2 cut(s) 24, 91
AfaI GTAC 3 cut(s) 144, 294, 476
AfiI CCNNNNNNNGG 3 cut(s) 122, 365, 470
AflII CTTAAG 1 cut(s) 58
AgsI TTSAA 1 cut(s) 391
AjnI CCWGG 1 cut(s) 295
AjuI GAANNNNNNNTTGG 2 cut(s) 148, 180
AluBI AGCT 2 cut(s) 190, 458
AluI AGCT 2 cut(s) 190, 458
AlwI GGATC 2 cut(s) 438, 451
ApoI RAATTY 1 cut(s) 450
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 2 cut(s) 129, 260
BamHI GGATCC 1 cut(s) 443
BccI CCATC 2 cut(s) 304, 434
BciT130I CCWGG 1 cut(s) 297
BcnI CCSGG 1 cut(s) 123
BfaI CTAG 3 cut(s) 168, 255, 363
BfrI CTTAAG 1 cut(s) 58
BglII AGATCT 1 cut(s) 384
Bme1390I CCNGG 2 cut(s) 123, 297
BmiI GGNNCC 1 cut(s) 445
BmrFI CCNGG 2 cut(s) 123, 297
BmsI GCATC 1 cut(s) 475
BpuMI CCSGG 1 cut(s) 123
BsaBI GATNNNNATC 1 cut(s) 314
BsaHI GRCGYC 2 cut(s) 24, 91
BsaJI CCNNGG 1 cut(s) 464
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 365, 470
Bse1I ACTGG 1 cut(s) 150
Bse8I GATNNNNATC 1 cut(s) 314
BseBI CCWGG 1 cut(s) 297
BseDI CCNNGG 1 cut(s) 464
BseGI GGATG 2 cut(s) 29, 71
BseJI GATNNNNATC 1 cut(s) 314
BseLI CCNNNNNNNGG 3 cut(s) 122, 365, 470
BseMII CTCAG 1 cut(s) 495
BseNI ACTGG 1 cut(s) 150
BseRI GAGGAG 2 cut(s) 348, 469
Bsh1236I CGCG 1 cut(s) 466
BsiSI CCGG 1 cut(s) 122
BslI CCNNNNNNNGG 3 cut(s) 122, 365, 470
Bsp143I GATC 3 cut(s) 372, 384, 443
BspACI CCGC 5 cut(s) 87, 407, 464, 466, 471
BspCNI CTCAG 1 cut(s) 494
BspFNI CGCG 1 cut(s) 466
BspLI GGNNCC 1 cut(s) 445
BspPI GGATC 2 cut(s) 438, 451
BspTI CTTAAG 1 cut(s) 58
BsrBI CCGCTC 1 cut(s) 471
BsrI ACTGG 1 cut(s) 150
BssECI CCNNGG 1 cut(s) 464
BssMI GATC 3 cut(s) 372, 384, 443
BssNI GRCGYC 2 cut(s) 24, 91
Bst2UI CCWGG 1 cut(s) 297
Bst4CI ACNGT 2 cut(s) 160, 292
Bst6I CTCTTC 1 cut(s) 169
BstACI GRCGYC 2 cut(s) 24, 91
BstAFI CTTAAG 1 cut(s) 58
BstC8I GCNNGC 4 cut(s) 85, 113, 188, 428
BstDEI CTNAG 1 cut(s) 481
BstDSI CCRYGG 1 cut(s) 464
BstENI CCTNNNNNAGG 1 cut(s) 363
BstF5I GGATG 2 cut(s) 29, 71
BstFNI CGCG 1 cut(s) 466
BstKTI GATC 3 cut(s) 375, 387, 446
BstMBI GATC 3 cut(s) 372, 384, 443
BstMWI GCNNNNNNNGC 1 cut(s) 348
BstNI CCWGG 1 cut(s) 297
BstNSI RCATGY 1 cut(s) 68
BstSCI CCNGG 2 cut(s) 121, 295
BstUI CGCG 1 cut(s) 466
BstX2I RGATCY 2 cut(s) 384, 443
BstYI RGATCY 2 cut(s) 384, 443
BtgI CCRYGG 1 cut(s) 464
BtsCI GGATG 2 cut(s) 29, 71
BtsI GCAGTG 1 cut(s) 337
BtsIMutI CAGTG 2 cut(s) 220, 337
Cac8I GCNNGC 4 cut(s) 85, 113, 188, 428
Cfr42I CCGCGG 1 cut(s) 467
CseI GACGC 2 cut(s) 32, 99
Csp6I GTAC 3 cut(s) 143, 293, 475
CviAII CATG 1 cut(s) 65
CviJI RGCY 5 cut(s) 7, 190, 300, 430, 458
CviKI_1 RGCY 5 cut(s) 7, 190, 300, 430, 458
CviQI GTAC 3 cut(s) 143, 293, 475
DdeI CTNAG 1 cut(s) 481
DpnI GATC 3 cut(s) 374, 386, 445
DpnII GATC 3 cut(s) 372, 384, 443
Eam1104I CTCTTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 387
EcoNI CCTNNNNNAGG 1 cut(s) 363
EcoRII CCWGG 1 cut(s) 295
FaeI CATG 1 cut(s) 68
FaiI YATR 5 cut(s) 66, 209, 223, 235, 237
FatI CATG 1 cut(s) 64
FauI CCCGC 1 cut(s) 464
FauNDI CATATG 1 cut(s) 235
FokI GGATG 2 cut(s) 16, 58
FspBI CTAG 3 cut(s) 168, 255, 363
HapII CCGG 1 cut(s) 122
HgaI GACGC 2 cut(s) 32, 99
Hin1I GRCGYC 2 cut(s) 24, 91
Hin1II CATG 1 cut(s) 68
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 1 cut(s) 323
HpaII CCGG 1 cut(s) 122
HphI GGTGA 2 cut(s) 129, 260
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 3 cut(s) 195, 484, 496
Hpy188III TCNNGA 2 cut(s) 376, 504
Hpy8I GTNNAC 1 cut(s) 133
HpyCH4III ACNGT 2 cut(s) 160, 292
HpyCH4V TGCA 1 cut(s) 83
HpyF10VI GCNNNNNNNGC 1 cut(s) 348
HpyF3I CTNAG 1 cut(s) 481
Hsp92I GRCGYC 2 cut(s) 24, 91
Hsp92II CATG 1 cut(s) 68
KspI CCGCGG 1 cut(s) 467
Kzo9I GATC 3 cut(s) 372, 384, 443
LmnI GCTCC 1 cut(s) 468
LpnPI CCDG 8 cut(s) 33, 131, 135, 253, 282, 309, 333, 468
LweI GCATC 1 cut(s) 475
MaeI CTAG 3 cut(s) 168, 255, 363
MaeIII GTNAC 1 cut(s) 160
MalI GATC 3 cut(s) 374, 386, 445
MbiI CCGCTC 1 cut(s) 471
MboI GATC 3 cut(s) 372, 384, 443
MboII GAAGA 5 cut(s) 186, 316, 319, 379, 449
MflI RGATCY 2 cut(s) 384, 443
MluCI AATT 2 cut(s) 117, 450
MmeI TCCRAC 3 cut(s) 179, 351, 357
MnlI CCTC 8 cut(s) 37, 44, 239, 359, 362, 366, 369, 490
MseI TTAA 2 cut(s) 59, 99
MspA1I CMGCKG 3 cut(s) 407, 458, 466
MspCI CTTAAG 1 cut(s) 58
MspI CCGG 1 cut(s) 122
MspR9I CCNGG 2 cut(s) 123, 297
MvaI CCWGG 1 cut(s) 297
MvnI CGCG 1 cut(s) 466
MwoI GCNNNNNNNGC 1 cut(s) 348
NciI CCSGG 1 cut(s) 123
NdeI CATATG 1 cut(s) 235
NdeII GATC 3 cut(s) 372, 384, 443
NlaIII CATG 1 cut(s) 68
NlaIV GGNNCC 1 cut(s) 445
NspI RCATGY 1 cut(s) 68
PfeI GAWTC 1 cut(s) 323
Psp6I CCWGG 1 cut(s) 295
PspGI CCWGG 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 445
PsuI RGATCY 2 cut(s) 384, 443
PvuII CAGCTG 1 cut(s) 458
RsaI GTAC 3 cut(s) 144, 294, 476
RsaNI GTAC 3 cut(s) 143, 293, 475
SacII CCGCGG 1 cut(s) 467
SaqAI TTAA 2 cut(s) 59, 99
Sau3AI GATC 3 cut(s) 372, 384, 443
ScrFI CCNGG 2 cut(s) 123, 297
SetI ASST 5 cut(s) 55, 192, 250, 272, 460
SfaNI GCATC 1 cut(s) 475
Sfr303I CCGCGG 1 cut(s) 467
SgrBI CCGCGG 1 cut(s) 467
SmlI CTYRAG 1 cut(s) 58
SmoI CTYRAG 1 cut(s) 58
Sse9I AATT 2 cut(s) 117, 450
SsiI CCGC 5 cut(s) 87, 407, 464, 466, 471
SspMI CTAG 3 cut(s) 168, 255, 363
StyD4I CCNGG 2 cut(s) 121, 295
TaaI ACNGT 2 cut(s) 160, 292
TaqI TCGA 1 cut(s) 302
TasI AATT 2 cut(s) 117, 450
TfiI GAWTC 1 cut(s) 323
Tru1I TTAA 2 cut(s) 59, 99
Tru9I TTAA 2 cut(s) 59, 99
TscAI CASTG 2 cut(s) 220, 344
TspDTI ATGAA 3 cut(s) 222, 252, 409
TspRI CASTG 2 cut(s) 220, 344
Vha464I CTTAAG 1 cut(s) 58
XagI CCTNNNNNAGG 1 cut(s) 363
XapI RAATTY 1 cut(s) 450
XceI RCATGY 1 cut(s) 68
XspI CTAG 3 cut(s) 168, 255, 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.