Rh5DG430800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004205
Physical Location & Seq
Forward (+)
68926866 .. 68930953
4088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG430800.1

Sequence Viewer

Length: 276 bp
ATGGAAAGCCCAGAATCCAACTCCAACCGCTGCCTAAAACGCCTCCCTCCTCCTAGAGGGCAGATCAAGAAAAAGATCTTCAAATCAGTGGTTTCAGCGGTGGGTGGGCTCATTAGCAAACCGAAAGAAGATGGATCCCAAATTCCAGCTGAACCGCGGAGCGGTGTTCAAGAAATTAGCATGTTATCAACCAGTATGCTAAAGTTCATGTGGACAATCAAAAGAATGGCCAAGAACATTGCTGCTAGGTTAATTCTAGCAAGTTTGTATCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

91

Amino Acids

10.07

Weight (kDa)

10.4

Isoelectric Point (pI)

52.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 162
AccII CGCG 1 cut(s) 157
AciI CCGC 5 cut(s) 28, 98, 155, 157, 162
AclWI GGATC 2 cut(s) 129, 142
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 1 cut(s) 141
AfiI CCNNNNNNNGG 2 cut(s) 56, 161
AgsI TTSAA 2 cut(s) 82, 170
AluBI AGCT 1 cut(s) 149
AluI AGCT 1 cut(s) 149
AlwI GGATC 2 cut(s) 129, 142
AoxI GGCC 1 cut(s) 228
ApeKI GCWGC 2 cut(s) 30, 242
ApoI RAATTY 1 cut(s) 141
BalI TGGCCA 1 cut(s) 230
BamHI GGATCC 1 cut(s) 134
BanII GRGCYC 1 cut(s) 111
BbvI GCAGC 2 cut(s) 17, 229
BccI CCATC 1 cut(s) 125
BfaI CTAG 3 cut(s) 54, 246, 257
BglII AGATCT 1 cut(s) 75
BisI GCNGC 2 cut(s) 31, 243
BlsI GCNGC 2 cut(s) 32, 244
BmiI GGNNCC 1 cut(s) 136
BsaJI CCNNGG 1 cut(s) 155
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 161
Bse1I ACTGG 1 cut(s) 192
Bse3DI GCAATG 1 cut(s) 237
BseDI CCNNGG 1 cut(s) 155
BseLI CCNNNNNNNGG 2 cut(s) 56, 161
BseMI GCAATG 1 cut(s) 237
BseNI ACTGG 1 cut(s) 192
BseRI GAGGAG 1 cut(s) 39
BseXI GCAGC 2 cut(s) 17, 229
Bsh1236I CGCG 1 cut(s) 157
BshFI GGCC 1 cut(s) 230
BslI CCNNNNNNNGG 2 cut(s) 56, 161
BsnI GGCC 1 cut(s) 230
Bsp1286I GDGCHC 1 cut(s) 111
Bsp143I GATC 3 cut(s) 63, 75, 134
BspACI CCGC 5 cut(s) 28, 98, 155, 157, 162
BspANI GGCC 1 cut(s) 230
BspFNI CGCG 1 cut(s) 157
BspLI GGNNCC 1 cut(s) 136
BspPI GGATC 2 cut(s) 129, 142
BsrBI CCGCTC 1 cut(s) 162
BsrDI GCAATG 1 cut(s) 237
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 1 cut(s) 155
BssMI GATC 3 cut(s) 63, 75, 134
BstDSI CCRYGG 1 cut(s) 155
BstENI CCTNNNNNAGG 1 cut(s) 54
BstFNI CGCG 1 cut(s) 157
BstKTI GATC 3 cut(s) 66, 78, 137
BstMBI GATC 3 cut(s) 63, 75, 134
BstMWI GCNNNNNNNGC 1 cut(s) 39
BstNSI RCATGY 1 cut(s) 184
BstUI CGCG 1 cut(s) 157
BstV1I GCAGC 2 cut(s) 17, 229
BstX2I RGATCY 2 cut(s) 75, 134
BstYI RGATCY 2 cut(s) 75, 134
BsuRI GGCC 1 cut(s) 230
BtgI CCRYGG 1 cut(s) 155
BtsIMutI CAGTG 1 cut(s) 93
Cfr42I CCGCGG 1 cut(s) 158
CviAII CATG 2 cut(s) 181, 208
CviJI RGCY 4 cut(s) 9, 109, 149, 230
CviKI_1 RGCY 4 cut(s) 9, 109, 149, 230
DpnI GATC 3 cut(s) 65, 77, 136
DpnII GATC 3 cut(s) 63, 75, 134
EaeI YGGCCR 1 cut(s) 228
Eco24I GRGCYC 1 cut(s) 111
EcoNI CCTNNNNNAGG 1 cut(s) 54
EcoT38I GRGCYC 1 cut(s) 111
FaeI CATG 2 cut(s) 184, 211
FaiI YATR 3 cut(s) 182, 197, 209
FatI CATG 2 cut(s) 180, 207
Fnu4HI GCNGC 2 cut(s) 31, 243
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 2 cut(s) 31, 243
FspBI CTAG 3 cut(s) 54, 246, 257
GluI GCNGC 2 cut(s) 31, 243
HaeIII GGCC 1 cut(s) 230
Hin1II CATG 2 cut(s) 184, 211
HinfI GANTC 1 cut(s) 14
Hpy166II GTNNAC 1 cut(s) 213
Hpy188III TCNNGA 2 cut(s) 67, 170
Hpy8I GTNNAC 1 cut(s) 213
HpyF10VI GCNNNNNNNGC 1 cut(s) 39
Hsp92II CATG 2 cut(s) 184, 211
KspI CCGCGG 1 cut(s) 158
Kzo9I GATC 3 cut(s) 63, 75, 134
LmnI GCTCC 1 cut(s) 159
LpnPI CCDG 3 cut(s) 24, 159, 205
Lsp1109I GCAGC 2 cut(s) 17, 229
MaeI CTAG 3 cut(s) 54, 246, 257
MalI GATC 3 cut(s) 65, 77, 136
MbiI CCGCTC 1 cut(s) 162
MboI GATC 3 cut(s) 63, 75, 134
MboII GAAGA 2 cut(s) 70, 140
MflI RGATCY 2 cut(s) 75, 134
MhlI GDGCHC 1 cut(s) 111
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 3 cut(s) 141, 174, 252
MluNI TGGCCA 1 cut(s) 230
MmeI TCCRAC 2 cut(s) 42, 48
MnlI CCTC 4 cut(s) 50, 53, 57, 60
Mox20I TGGCCA 1 cut(s) 230
MscI TGGCCA 1 cut(s) 230
MseI TTAA 2 cut(s) 251, 274
Msp20I TGGCCA 1 cut(s) 230
MspA1I CMGCKG 4 cut(s) 30, 98, 149, 157
MvnI CGCG 1 cut(s) 157
MwoI GCNNNNNNNGC 1 cut(s) 39
NdeII GATC 3 cut(s) 63, 75, 134
NlaIII CATG 2 cut(s) 184, 211
NlaIV GGNNCC 1 cut(s) 136
NspI RCATGY 1 cut(s) 184
PfeI GAWTC 1 cut(s) 14
PkrI GCNGC 2 cut(s) 32, 244
PspN4I GGNNCC 1 cut(s) 136
PsuI RGATCY 2 cut(s) 75, 134
PvuII CAGCTG 1 cut(s) 149
SacII CCGCGG 1 cut(s) 158
SaqAI TTAA 2 cut(s) 251, 274
SatI GCNGC 2 cut(s) 31, 243
Sau3AI GATC 3 cut(s) 63, 75, 134
SduI GDGCHC 1 cut(s) 111
SetI ASST 2 cut(s) 151, 251
Sfr303I CCGCGG 1 cut(s) 158
SgrBI CCGCGG 1 cut(s) 158
Sse9I AATT 3 cut(s) 141, 174, 252
SsiI CCGC 5 cut(s) 28, 98, 155, 157, 162
SspMI CTAG 3 cut(s) 54, 246, 257
TasI AATT 3 cut(s) 141, 174, 252
TfiI GAWTC 1 cut(s) 14
Tru1I TTAA 2 cut(s) 251, 274
Tru9I TTAA 2 cut(s) 251, 274
TscAI CASTG 1 cut(s) 93
TseI GCWGC 2 cut(s) 30, 242
TspDTI ATGAA 1 cut(s) 196
TspRI CASTG 1 cut(s) 93
XagI CCTNNNNNAGG 1 cut(s) 54
XapI RAATTY 1 cut(s) 141
XceI RCATGY 1 cut(s) 184
XspI CTAG 3 cut(s) 54, 246, 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.