Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0002978

Physical Location & Seq

Genomic Coordinates Forward (+)

78210955 .. 78214237

Gene Structure 3283 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0585100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3234 bp

ATGGCCGTCAGTGTCAAGAAGCTAGAGTACCTTTCCTTGATTTCGAAAGTCTGTTCGGAACTCGAAACCAATCTAGGGCAGCAGTATGGGGACAAAGCCCTAGCTGAGTTCATAACCGATATGGGACGGAACTCCAAGAACGTGGACGAATTCAATGCCAAGTTGAATGAATTTGGCGCCGAAATCCCTGATTACTTTGTCCGTAATCTTCTCACTATTATTCACGCAGTTCTTGCTCCCAACAAGAACCAGAGCTCTTCTAAAGAAGAAAAAGAAGACAAGCCTAGAGATAGAAGTAGAGAAAGCAGAGGTAAGAATAAGGATCAAGAGTACAATTCCGGTGAGCCGGAGTTGTATGGAGTTTATAAGGGCAGGGTTTCGAAAGTGATGGATGCAGGTTGCTTTGTTGAGTTGAGTTGTTTTAGAGGGAAGGAGGGTTTGGTTCATGTTTCGCAGATTGCGAAGAGGCGAATTGGTAATGCTAAAGATGTGGTGAAGAGAGATCAGCAAGTTTATGTGAAGGTGGTTTCGATTTCGGGTCAGAAGTTGAGTCTTTCAATGAAGGATGTTGATCAGAAGACAGGGAAGGATTTACTTCCTTTGAAGAAGAGCTCAGATTATGATGCTACTAGGTCGAACCCGTCAATGTCAAAACATGGTTCTGTGAGTAGGACTAGTCTTTCTGGGATTAGGACTGTGGAGGAGGATAGTTTTGTCCAATCTCGTAGGCCAAGGAAGAGAATGAGCTCCCCAGAGAGGTGGGAAATCAAACAGTTGATTGCTTCAGGTGTTTTGAGTGCTAAAGAGTATCCAATGCTTTATCAAGATGAGGAAGCGGATGGCATAATGATGAAGCAGCTCTATAAAGATGAGGGACCTGAGGAAGAGCTTGAAATCGAGCTCAATGAGGAAGCACCGGCCTTTTTGCAAGGGCACAATATGTATTCTGTGGATATGTCGCCGGTGAAGATCTTTAAGAATCCAGAAGGGTCACTGAGCCGTGCAGCTGCACTTCAATCTGCACTGATCAAGGAGCGCCGAGAAATGAGAGATCAGCAGCAAAGAGCAGTGCTGGATTCTATCCCGAAGGACCTGAATCGTCCGTGGGAAGATCCAATGGCACAGAATGGTGAGAGGCATCTTGCACAGGAACTTAGAGGTGTTGGTTTGTCAGCTTATGACATGCCTGAGTGGAAGAAGGATACTTATGCGAAAGCTATGAGTTTCGGGAAGAGGTCAAGCCTCTCGATTCAGGAGCAAAGGAAGAGCTTGCCTGTCTACAAGCTGAGGGAAGAACTGATTAAGGCAGTGAATGAGAATCAAGTGCTTATTGTCATTGGTGAGACTGGTTCTGGTAAGACAACTCAGCTAACACAGTATCTTGCAGAAGCAGGTTACACGACAAAGGGTAGGATTGGATGTACGCAGCCTCGTAGGGTGGCGGCAATGTCAGTGGCGAAAAGGGTTGCTGAAGAGGTTGGTTGTCGGTTGGGGGAGGAAGTCGGTTATGCCATTCGTTTTGAGGATTGCACTGGACCAGATACTGTCATCAAGTACATGACAGATGGTATGCTTCTTAGGGAGATTTTGATGGATGAGAACCTTTCGCAATACTCTGTGATAATGCTCGATGAAGCTCATGAGAGGACAATCCACACGGATGTTCTTTTTGGATTACTGAAGCAACTTTTGAAGCGGAGACCAGACCTTCGTTTGATTGTTACCTCTGCTACTTTGGATGCAGAGAAGTTTTCAGGTTATTTCTTCAACTGCATGCTCTTCTCTATCCCTGGGAGAACTTTTCCATACAAGGTAAACTATGTCAAGGAGCCAGAAAGTGATTACCTTGATGCGGCTCTAATCACTGTTTTACAGATCCACTTGGAAGAACCTGAGGGTGACATCCTTCTCTTCTTGACTGGACAGGAAGAGATTGATTTTGCATGTCAGATTCTTTATGAGAGGATGAAAGCTCTTGGTAAAAATGTACCCGAGTTGATTATCCTACCAGTTTATAGTGCTCTTCCTAGTGAAATTCAGTCGAGGATATTTGATCCAGCCCCGCCGGGGAAGCGGAAAGTAGTTGTGGCTACTAACATTGCCGAGGCATCTCTGACTATTGATGGAATATTTTATGTCGTTGATCCTGGATTTGCAAAACAAAATGTTTATAACCCAAAGCTGGGGCTAGATTCACTGGTCATAACTCCGATTTCACAAGCATCAGCAAAGCAACGAGCTGGGCGTGCTGGGCGTACAGGCCCTGGGAAATGTTACCGTCTCTACACTGAGAGTGCATTCCGCAATGAGATGCCCCCTACTACAATTCCCGAAATCCAGAGGACAAATCTTGGGTCTACGGTGCTTGCAATGAAAGCAATGGGGATAAACGATCTCCTGTCTTTTGATTTTATGGATCCTCCTGCGCCGCAGGCACTCATTTCAGCAATGGAACAACTGTACAGCTTAGGAGCACTTGATGAAGAGGGTTTCCTAACCAGACTAGGAAGGAAAATGTCAGAGTTTCCTCTGGATCCACCCCTCTCGAAGATGCTCCTCGCCAGTGTGGACCTCGGATGCAGTGATGAGATCCTGACCATCATAGCAATGATTCAGACAGGCAATGTCTTTTACAGGCCTAGGGAGAAACAAGACCGGGCAGATCAGAAGAGAGCAAAGTTTTTCCAGCCAGAAGGAGACCATCTTACTTTACTTGAAGTCTACAAGGCTTGGAAGAGAACCAACTTTTCAGCTCCATGGTGTTTCGACAACTTTCTTCAGTCTCGATCCTTGAGGCGCGCACAGGATGTGAGAAAACAGCTAATGAGCATCATGGATAAGCACAAGCTGGATGTTGTCAGTGCCGGAAACAATTACACAAAGATTAGCAAGGCAATCACGGCCGGCTTTTTCTTCCATGTTGCTAAAAAGGATCCACAGGAAGGTTATAAGACCCTAGTTGAGAGCCAGCCAGTGTACATCCATCCAAGCAGTGCTCTCTTCCAGAAGCAACCAGATTGGGTCATCTACAATGAGCTGGTTATGACTACAAAGGAGTACATGCGCGAGGTGACAGCCATTGATCCCAAATGGCTTGTGGAATTGGCACCAAGGTTCTTCAAAGCTGCAGATCCTAACAAGATGACCAAGCGCAAGCGTCAACAACGTATTGAACCATTGTATGACAGATACAATGAACCCAACTCTTGGCGTCTTAGTAGACGCCGTGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000413 GO:0003006 GO:0003674 GO:0003755 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009791 GO:0009888 GO:0009908 GO:0009987 GO:0010022 GO:0010050 GO:0010073 GO:0010582 GO:0016853 GO:0016859 GO:0018193 GO:0018208 GO:0019538 GO:0022414 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048367 GO:0048507 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090567 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04217 ko04218 map04217 map04218

Pfam Domains

Protein Families

Pro_isomerase TPR_1 TPR_2

Protein Analysis

1077

Amino Acids

122.14

Weight (kDa)

8.61

Isoelectric Point (pI)

51.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
S1	PF00575	121 - 187	1.5e-10	S1 RNA binding domain
DEAD	PF00270	431 - 580	3.1e-08	DEAD/DEAH box helicase
AAA_22	PF13401	440 - 572	3.3e-06	AAA domain
Helicase_C	PF00271	623 - 754	1.5e-10	Helicase conserved C-terminal domain
WHD_HA2	PF04408	816 - 844	7.4e-11	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	845 - 904	6.7e-15	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	962 - 1038	3.4e-27	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002978)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G15790
fragaria_vesca	FvH4_1g15560 FvH4_1g15560 FvH4_1g15560 FvH4_2g06830 FvH4_2g06830 FvH4_2g06830 FvH4_2g06830 FvH4_2g06830 FvH4_2g06830
malus_domestica	MD02G1167800.v1.1 MD05G1075200.v1.1 MD15G1280000.v1.1
prunus_persica	Prupe.7G137400_v2.0.a1 Prupe.8G116600_v2.0.a1 Prupe.8G116600_v2.0.a1
pyrus_communis	pycom02g13470 pycom05g06840
rosa_chinensis	RchiOBHm_Chr2g0104891 RchiOBHm_Chr6g0259041
rosa_laevigata	RLG00000014561 RLG00000017365
rosa_multiflora	Rmu_sc0003419.1_g000006 Rmu_ssc0000050.1_g000113
rosa_roxburghii	Rroxscaffold_2G00138700 Rroxscaffold_7G00206290 Rroxscaffold_7G00206440
rosa_rugosa	Rorug02G0123600 Rorug02G0123700 Rorug05G0585100 Rorug05G0585200
rosa_samantha	Rh2AG173900 Rh2BG181500 Rh2CG178800 Rh2DG180200 Rh6AG101400
rosa_wichuraiana	Rw2G013680 Rw6G008820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	366, 2172, 2949
Acc36I	ACCTGC	2 cut(s)	386, 1382
AccB1I	GGYRCC	2 cut(s)	176, 3106
AccB7I	CCANNNNNTGG	1 cut(s)	3207
AccI	GTMKAC	4 cut(s)	1278, 2357, 2721, 3220
AccII	CGCG	2 cut(s)	2799, 3066
AciI	CCGC	8 cut(s)	836, 1442, 1696, 1853, 2063, 2074, 2302, 2429
AcoI	YGGCCR	2 cut(s)	3, 2901
AcsI	RAATTY	3 cut(s)	149, 170, 2034
AcuI	CTGAAG	4 cut(s)	768, 1491, 1700, 2762
AcyI	GRCGYC	3 cut(s)	177, 3211, 3223
AfaI	GTAC	9 cut(s)	29, 332, 1423, 1556, 1989, 2257, 2462, 2978, 3059
AfiI	CCNNNNNNNGG	8 cut(s)	75, 756, 1852, 2067, 2182, 2183, 2942, 3207
AhlI	ACTAGT	1 cut(s)	674
AjnI	CCWGG	3 cut(s)	1789, 2146, 2263
AjuI	GAANNNNNNNTTGG	2 cut(s)	422, 454
Alw21I	GWGCWC	7 cut(s)	257, 614, 749, 903, 2023, 2476, 2998
Alw26I	GTCTC	5 cut(s)	1337, 1693, 2285, 2691, 2787
AlwNI	CAGNNNCTG	2 cut(s)	1544, 2264
Ama87I	CYCGRG	1 cut(s)	1991
AoxI	GGCC	6 cut(s)	3, 728, 918, 2260, 2636, 2901
ApeKI	GCWGC	7 cut(s)	79, 856, 1004, 1007, 1057, 1426, 3125
ApoI	RAATTY	3 cut(s)	149, 170, 2034
ArsI	GACNNNNNNTTYG	4 cut(s)	1692, 1724, 2339, 2371
AspA2I	CCTAGG	1 cut(s)	2639
AspLEI	GCGC	7 cut(s)	179, 1038, 2428, 2799, 2801, 3066, 3153
AspS9I	GGNCC	5 cut(s)	875, 1090, 1535, 2261, 2569
AsuC2I	CCSGG	2 cut(s)	2067, 2657
AsuHPI	GGTGA	7 cut(s)	353, 505, 976, 1142, 1352, 1910, 3082
AsuII	TTCGAA	2 cut(s)	44, 380
AvaI	CYCGRG	1 cut(s)	1991
AvaII	GGWCC	4 cut(s)	875, 1090, 1535, 2569
AvrII	CCTAGG	1 cut(s)	2639
AxyI	CCTNAGG	2 cut(s)	879, 1893
BaeGI	GKGCMC	1 cut(s)	936
BamHI	GGATCC	3 cut(s)	2416, 2533, 2932
BanI	GGYRCC	2 cut(s)	176, 3106
BanII	GRGCYC	4 cut(s)	257, 614, 749, 903
BarI	GAAGNNNNNNTAC	2 cut(s)	11, 43
BbsI	GAAGAC	2 cut(s)	282, 584
Bbv12I	GWGCWC	7 cut(s)	257, 614, 749, 903, 2023, 2476, 2998
BbvCI	CCTCAGC	1 cut(s)	1286
BbvI	GCAGC	7 cut(s)	91, 868, 994, 1016, 1069, 1438, 3112
BccI	CCATC	8 cut(s)	382, 833, 1559, 1585, 2117, 2606, 2709, 2991
BceAI	ACGGC	3 cut(s)	984, 2916, 3210
BcgI	CGANNNNNNTGC	2 cut(s)	137, 171
BciT130I	CCWGG	3 cut(s)	1791, 2148, 2265
BciVI	GTATCC	2 cut(s)	819, 1195
BclI	TGATCA	2 cut(s)	571, 1026
BcnI	CCSGG	2 cut(s)	2067, 2657
BcoDI	GTCTC	5 cut(s)	1337, 1693, 2285, 2691, 2787
BcuI	ACTAGT	1 cut(s)	674
BfmI	CTRYAG	1 cut(s)	3126
BfoI	RGCGCY	2 cut(s)	180, 1039
BfuAI	ACCTGC	2 cut(s)	386, 1382
BfuI	GTATCC	2 cut(s)	819, 1195
BglII	AGATCT	1 cut(s)	969
BlnI	CCTAGG	1 cut(s)	2639
Bme1390I	CCNGG	5 cut(s)	1791, 2067, 2148, 2265, 2657
Bme18I	GGWCC	4 cut(s)	875, 1090, 1535, 2569
BmeT110I	CYCGRG	1 cut(s)	1991
BmgT120I	GGNCC	5 cut(s)	875, 1090, 1535, 2261, 2569
BmiI	GGNNCC	7 cut(s)	178, 876, 1830, 2418, 2535, 2934, 3108
BmrFI	CCNGG	5 cut(s)	1791, 2067, 2148, 2265, 2657
BpiI	GAAGAC	2 cut(s)	282, 584
Bpu10I	CCTNAGC	2 cut(s)	1286, 2467
Bpu14I	TTCGAA	2 cut(s)	44, 380
BpuEI	CTTGAG	1 cut(s)	2812
BpuMI	CCSGG	2 cut(s)	2067, 2657
BsaBI	GATNNNNATC	1 cut(s)	570
BsaHI	GRCGYC	3 cut(s)	177, 3211, 3223
BsaI	GGTCTC	2 cut(s)	1693, 2691
BsaWI	WCCGGW	1 cut(s)	338
Bsc4I	CCNNNNNNNGG	8 cut(s)	75, 756, 1852, 2067, 2182, 2183, 2942, 3207
Bse118I	RCCGGY	3 cut(s)	916, 961, 2903
Bse1I	ACTGG	7 cut(s)	1351, 1537, 1924, 2009, 2202, 2562, 2972
Bse21I	CCTNAGG	2 cut(s)	879, 1893
Bse3DI	GCAATG	8 cut(s)	1452, 2097, 2311, 2376, 2385, 2454, 2613, 2629
Bse8I	GATNNNNATC	1 cut(s)	570
BseBI	CCWGG	3 cut(s)	1791, 2148, 2265
BseJI	GATNNNNATC	1 cut(s)	570
BseLI	CCNNNNNNNGG	8 cut(s)	75, 756, 1852, 2067, 2182, 2183, 2942, 3207
BseMI	GCAATG	8 cut(s)	1452, 2097, 2311, 2376, 2385, 2454, 2613, 2629
BseMII	CTCAG	9 cut(s)	96, 627, 870, 986, 1179, 1277, 1379, 1884, 2280
BseNI	ACTGG	7 cut(s)	1351, 1537, 1924, 2009, 2202, 2562, 2972
BsePI	GCGCGC	1 cut(s)	2797
BseRI	GAGGAG	2 cut(s)	716, 2546
BseSI	GKGCMC	1 cut(s)	936
BseX3I	CGGCCG	1 cut(s)	2901
BseXI	GCAGC	7 cut(s)	91, 868, 994, 1016, 1069, 1438, 3112
BseYI	CCCAGC	3 cut(s)	2182, 2240, 2249
BsgI	GTGCAG	3 cut(s)	993, 1005, 1023
Bsh1236I	CGCG	2 cut(s)	2799, 3066
Bsh1285I	CGRYCG	1 cut(s)	2904
BshFI	GGCC	6 cut(s)	5, 730, 920, 2262, 2638, 2903
BshNI	GGYRCC	2 cut(s)	176, 3106
BsiEI	CGRYCG	1 cut(s)	2904
BsiHKAI	GWGCWC	7 cut(s)	257, 614, 749, 903, 2023, 2476, 2998
BsiHKCI	CYCGRG	1 cut(s)	1991
BsiSI	CCGG	8 cut(s)	339, 347, 917, 962, 2066, 2656, 2865, 2904
BslFI	GGGAC	3 cut(s)	104, 138, 888
BslI	CCNNNNNNNGG	8 cut(s)	75, 756, 1852, 2067, 2182, 2183, 2942, 3207
BsmAI	GTCTC	5 cut(s)	1337, 1693, 2285, 2691, 2787
BsmBI	CGTCTC	1 cut(s)	2285
BsmFI	GGGAC	3 cut(s)	104, 138, 888
BsmI	GAATGC	1 cut(s)	2297
BsnI	GGCC	6 cut(s)	5, 730, 920, 2262, 2638, 2903
Bso31I	GGTCTC	2 cut(s)	1693, 2691
BsoBI	CYCGRG	1 cut(s)	1991
Bsp119I	TTCGAA	2 cut(s)	44, 380
Bsp1286I	GDGCHC	8 cut(s)	257, 614, 749, 903, 936, 2023, 2476, 2998
Bsp1407I	TGTACA	2 cut(s)	2460, 2976
Bsp19I	CCATGG	1 cut(s)	2756
BspACI	CCGC	8 cut(s)	836, 1442, 1696, 1853, 2063, 2074, 2302, 2429
BspANI	GGCC	6 cut(s)	5, 730, 920, 2262, 2638, 2903
BspCNI	CTCAG	9 cut(s)	97, 626, 871, 987, 1180, 1278, 1378, 1885, 2281
BspFNI	CGCG	2 cut(s)	2799, 3066
BspHI	TCATGA	1 cut(s)	1639
BspLI	GGNNCC	7 cut(s)	178, 876, 1830, 2418, 2535, 2934, 3108
BspMAI	CTGCAG	1 cut(s)	3130
BspMI	ACCTGC	2 cut(s)	386, 1382
BspQI	GCTCTTC	6 cut(s)	262, 602, 879, 1259, 1784, 2028
BspT104I	TTCGAA	2 cut(s)	44, 380
BspT107I	GGYRCC	2 cut(s)	176, 3106
BspTNI	GGTCTC	2 cut(s)	1693, 2691
BsrDI	GCAATG	8 cut(s)	1452, 2097, 2311, 2376, 2385, 2454, 2613, 2629
BsrFI	RCCGGY	3 cut(s)	916, 961, 2903
BsrGI	TGTACA	2 cut(s)	2460, 2976
BsrI	ACTGG	7 cut(s)	1351, 1537, 1924, 2009, 2202, 2562, 2972
BssAI	RCCGGY	3 cut(s)	916, 961, 2903
BssHII	GCGCGC	1 cut(s)	2797
BssNI	GRCGYC	3 cut(s)	177, 3211, 3223
BssT1I	CCWWGG	4 cut(s)	731, 2639, 2756, 3110
Bst2UI	CCWGG	3 cut(s)	1791, 2148, 2265
Bst4CI	ACNGT	8 cut(s)	697, 774, 1377, 1546, 1867, 2279, 2362, 2460
BstACI	GRCGYC	3 cut(s)	177, 3211, 3223
BstAPI	GCANNNNNTGC	1 cut(s)	233
BstAUI	TGTACA	2 cut(s)	2460, 2976
BstBI	TTCGAA	2 cut(s)	44, 380
BstC8I	GCNNGC	9 cut(s)	1271, 1775, 2247, 2367, 2433, 2799, 2905, 2969, 3155
BstDSI	CCRYGG	3 cut(s)	1103, 2756, 3226
BstFNI	CGCG	2 cut(s)	2799, 3066
BstH2I	RGCGCY	2 cut(s)	180, 1039
BstHHI	GCGC	7 cut(s)	179, 1038, 2428, 2799, 2801, 3066, 3153
BstMAI	GTCTC	5 cut(s)	1337, 1693, 2285, 2691, 2787
BstMCI	CGRYCG	1 cut(s)	2904
BstMWI	GCNNNNNNNGC	7 cut(s)	233, 2071, 2246, 2251, 2375, 2432, 2900
BstNI	CCWGG	3 cut(s)	1791, 2148, 2265
BstNSI	RCATGY	4 cut(s)	1186, 1777, 1947, 3064
BstSCI	CCNGG	5 cut(s)	1789, 2065, 2146, 2263, 2655
BstSFI	CTRYAG	1 cut(s)	3126
BstSLI	GKGCMC	1 cut(s)	936
BstUI	CGCG	2 cut(s)	2799, 3066
BstV1I	GCAGC	7 cut(s)	91, 868, 994, 1016, 1069, 1438, 3112
BstV2I	GAAGAC	2 cut(s)	282, 584
BstX2I	RGATCY	8 cut(s)	969, 1111, 1875, 2416, 2533, 2589, 2932, 3130
BstXI	CCANNNNNNTGG	2 cut(s)	142, 759
BstYI	RGATCY	8 cut(s)	969, 1111, 1875, 2416, 2533, 2589, 2932, 3130
BstZI	CGGCCG	1 cut(s)	2901
Bsu36I	CCTNAGG	2 cut(s)	879, 1893
BsuI	GTATCC	2 cut(s)	819, 1195
BsuRI	GGCC	6 cut(s)	5, 730, 920, 2262, 2638, 2903
BtgI	CCRYGG	3 cut(s)	1103, 2756, 3226
BtsI	GCAGTG	4 cut(s)	1074, 1314, 2587, 2998
BveI	ACCTGC	2 cut(s)	386, 1382
Cac8I	GCNNGC	9 cut(s)	1271, 1775, 2247, 2367, 2433, 2799, 2905, 2969, 3155
CaiI	CAGNNNCTG	2 cut(s)	1544, 2264
CciI	TCATGA	1 cut(s)	1639
CfoI	GCGC	7 cut(s)	179, 1038, 2428, 2799, 2801, 3066, 3153
Cfr10I	RCCGGY	3 cut(s)	916, 961, 2903
Cfr13I	GGNCC	5 cut(s)	875, 1090, 1535, 2261, 2569
CseI	GACGC	2 cut(s)	3146, 3200
Csp6I	GTAC	9 cut(s)	28, 331, 1422, 1555, 1988, 2256, 2461, 2977, 3058
CspCI	CAANNNNNGTGG	2 cut(s)	1434, 1469
CviQI	GTAC	9 cut(s)	28, 331, 1422, 1555, 1988, 2256, 2461, 2977, 3058
DinI	GGCGCC	1 cut(s)	178
EaeI	YGGCCR	2 cut(s)	3, 2901
EagI	CGGCCG	1 cut(s)	2901
Ecl136II	GAGCTC	4 cut(s)	255, 612, 747, 901
EclXI	CGGCCG	1 cut(s)	2901
Eco130I	CCWWGG	4 cut(s)	731, 2639, 2756, 3110
Eco147I	AGGCCT	1 cut(s)	2638
Eco24I	GRGCYC	4 cut(s)	257, 614, 749, 903
Eco31I	GGTCTC	2 cut(s)	1693, 2691
Eco47I	GGWCC	4 cut(s)	875, 1090, 1535, 2569
Eco52I	CGGCCG	1 cut(s)	2901
Eco53kI	GAGCTC	4 cut(s)	255, 612, 747, 901
Eco57I	CTGAAG	4 cut(s)	768, 1491, 1700, 2762
Eco81I	CCTNAGG	2 cut(s)	879, 1893
Eco88I	CYCGRG	1 cut(s)	1991
EcoICRI	GAGCTC	4 cut(s)	255, 612, 747, 901
EcoO109I	RGGNCCY	3 cut(s)	875, 1090, 2261
EcoRI	GAATTC	1 cut(s)	149
EcoRII	CCWGG	3 cut(s)	1789, 2146, 2263
EcoT14I	CCWWGG	4 cut(s)	731, 2639, 2756, 3110
EcoT38I	GRGCYC	4 cut(s)	257, 614, 749, 903
EgeI	GGCGCC	1 cut(s)	178
EheI	GGCGCC	1 cut(s)	178
ErhI	CCWWGG	4 cut(s)	731, 2639, 2756, 3110
Esp3I	CGTCTC	1 cut(s)	2285
FaqI	GGGAC	3 cut(s)	104, 138, 888
FauI	CCCGC	1 cut(s)	2070
FbaI	TGATCA	2 cut(s)	571, 1026
FblI	GTMKAC	4 cut(s)	1278, 2357, 2721, 3220
FriOI	GRGCYC	4 cut(s)	257, 614, 749, 903
GlaI	GCGC	7 cut(s)	178, 1037, 2427, 2798, 2800, 3065, 3152
GsaI	CCCAGC	3 cut(s)	2186, 2244, 2253
HaeII	RGCGCY	2 cut(s)	180, 1039
HaeIII	GGCC	6 cut(s)	5, 730, 920, 2262, 2638, 2903
HapII	CCGG	8 cut(s)	339, 347, 917, 962, 2066, 2656, 2865, 2904
HgaI	GACGC	2 cut(s)	3146, 3200
HhaI	GCGC	7 cut(s)	179, 1038, 2428, 2799, 2801, 3066, 3153
Hin1I	GRCGYC	3 cut(s)	177, 3211, 3223
Hin6I	GCGC	7 cut(s)	177, 1036, 2426, 2797, 2799, 3064, 3151
HinP1I	GCGC	7 cut(s)	177, 1036, 2426, 2797, 2799, 3064, 3151
HincII	GTYRAC	1 cut(s)	3161
HindII	GTYRAC	1 cut(s)	3161
HinfI	GANTC	9 cut(s)	550, 979, 1076, 1096, 1249, 1318, 1951, 2192, 2611
HpaII	CCGG	8 cut(s)	339, 347, 917, 962, 2066, 2656, 2865, 2904
HphI	GGTGA	7 cut(s)	353, 505, 976, 1142, 1352, 1910, 3082
Hpy166II	GTNNAC	9 cut(s)	145, 1279, 1816, 2358, 2569, 2722, 2977, 3161, 3221
Hpy8I	GTNNAC	9 cut(s)	145, 1279, 1816, 2358, 2569, 2722, 2977, 3161, 3221
HpyCH4III	ACNGT	8 cut(s)	697, 774, 1377, 1546, 1867, 2279, 2362, 2460
HpyCH4IV	ACGT	2 cut(s)	141, 3166
HpyF10VI	GCNNNNNNNGC	7 cut(s)	233, 2071, 2246, 2251, 2375, 2432, 2900
HpySE526I	ACGT	2 cut(s)	141, 3166
Hsp92I	GRCGYC	3 cut(s)	177, 3211, 3223
HspAI	GCGC	7 cut(s)	177, 1036, 2426, 2797, 2799, 3064, 3151
KasI	GGCGCC	1 cut(s)	176
KroI	GCCGGC	1 cut(s)	2903
KroNI	GCCGGC	1 cut(s)	2905
Ksp22I	TGATCA	2 cut(s)	571, 1026
LguI	GCTCTTC	6 cut(s)	262, 602, 879, 1259, 1784, 2028
LmnI	GCTCC	8 cut(s)	241, 752, 1033, 1255, 1828, 2471, 2559, 2758
Lsp1109I	GCAGC	7 cut(s)	91, 868, 994, 1016, 1069, 1438, 3112
MaeII	ACGT	2 cut(s)	141, 3166
MaeIII	GTNAC	6 cut(s)	990, 1394, 1720, 1898, 2273, 3070
MflI	RGATCY	8 cut(s)	969, 1111, 1875, 2416, 2533, 2589, 2932, 3130
MhlI	GDGCHC	8 cut(s)	257, 614, 749, 903, 936, 2023, 2476, 2998
MluCI	AATT	8 cut(s)	149, 170, 334, 471, 2034, 2325, 2872, 3101
Mly113I	GGCGCC	1 cut(s)	177
MlyI	GAGTC	1 cut(s)	559
MroNI	GCCGGC	1 cut(s)	2903
MseI	TTAA	2 cut(s)	975, 1302
MslI	CAYNNNNRTG	3 cut(s)	848, 1659, 2606
MspA1I	CMGCKG	1 cut(s)	1007
MspI	CCGG	8 cut(s)	339, 347, 917, 962, 2066, 2656, 2865, 2904
MspR9I	CCNGG	5 cut(s)	1791, 2067, 2148, 2265, 2657
Mva1269I	GAATGC	1 cut(s)	2297
MvaI	CCWGG	3 cut(s)	1791, 2148, 2265
MvnI	CGCG	2 cut(s)	2799, 3066
MwoI	GCNNNNNNNGC	7 cut(s)	233, 2071, 2246, 2251, 2375, 2432, 2900
NaeI	GCCGGC	1 cut(s)	2905
NarI	GGCGCC	1 cut(s)	177
NciI	CCSGG	2 cut(s)	2067, 2657
NcoI	CCATGG	1 cut(s)	2756
NgoMIV	GCCGGC	1 cut(s)	2903
NlaIV	GGNNCC	7 cut(s)	178, 876, 1830, 2418, 2535, 2934, 3108
NmeAIII	GCCGAG	2 cut(s)	1064, 2128
NmuCI	GTSAC	3 cut(s)	990, 1898, 3070
NspI	RCATGY	4 cut(s)	1186, 1777, 1947, 3064
NspV	TTCGAA	2 cut(s)	44, 380
PaeI	GCATGC	1 cut(s)	1777
PagI	TCATGA	1 cut(s)	1639
PasI	CCCWGGG	2 cut(s)	1790, 2264
PauI	GCGCGC	1 cut(s)	2797
PceI	AGGCCT	1 cut(s)	2638
PciSI	GCTCTTC	6 cut(s)	262, 602, 879, 1259, 1784, 2028
PcsI	WCGNNNNNNNCGW	2 cut(s)	458, 2242
PctI	GAATGC	1 cut(s)	2297
PdiI	GCCGGC	1 cut(s)	2905
PfeI	GAWTC	8 cut(s)	979, 1076, 1096, 1249, 1318, 1951, 2192, 2611
PflMI	CCANNNNNTGG	1 cut(s)	3207
PfoI	TCCNGGA	1 cut(s)	2146
PleI	GAGTC	1 cut(s)	558
PluTI	GGCGCC	1 cut(s)	180
PpsI	GAGTC	1 cut(s)	558
PpuMI	RGGWCCY	2 cut(s)	875, 1090
PsiI	TTATAA	3 cut(s)	366, 2172, 2949
Psp124BI	GAGCTC	4 cut(s)	257, 614, 749, 903
Psp5II	RGGWCCY	2 cut(s)	875, 1090
Psp6I	CCWGG	3 cut(s)	1789, 2146, 2263
PspFI	CCCAGC	3 cut(s)	2182, 2240, 2249
PspGI	CCWGG	3 cut(s)	1789, 2146, 2263
PspN4I	GGNNCC	7 cut(s)	178, 876, 1830, 2418, 2535, 2934, 3108
PspPI	GGNCC	5 cut(s)	875, 1090, 1535, 2261, 2569
PspPPI	RGGWCCY	2 cut(s)	875, 1090
PstI	CTGCAG	1 cut(s)	3130
PstNI	CAGNNNCTG	2 cut(s)	1544, 2264
PsuI	RGATCY	8 cut(s)	969, 1111, 1875, 2416, 2533, 2589, 2932, 3130
PteI	GCGCGC	1 cut(s)	2797
PvuII	CAGCTG	1 cut(s)	1007
RsaI	GTAC	9 cut(s)	29, 332, 1423, 1556, 1989, 2257, 2462, 2978, 3059
RsaNI	GTAC	9 cut(s)	28, 331, 1422, 1555, 1988, 2256, 2461, 2977, 3058
RseI	CAYNNNNRTG	3 cut(s)	848, 1659, 2606
SacI	GAGCTC	4 cut(s)	257, 614, 749, 903
SapI	GCTCTTC	6 cut(s)	262, 602, 879, 1259, 1784, 2028
SaqAI	TTAA	2 cut(s)	975, 1302
Sau96I	GGNCC	5 cut(s)	875, 1090, 1535, 2261, 2569
SchI	GAGTC	1 cut(s)	559
ScrFI	CCNGG	5 cut(s)	1791, 2067, 2148, 2265, 2657
SduI	GDGCHC	8 cut(s)	257, 614, 749, 903, 936, 2023, 2476, 2998
SfcI	CTRYAG	1 cut(s)	3126
SfoI	GGCGCC	1 cut(s)	178
SfuI	TTCGAA	2 cut(s)	44, 380
SgrAI	CRCCGGYG	1 cut(s)	961
SinI	GGWCC	4 cut(s)	875, 1090, 1535, 2569
SmiMI	CAYNNNNRTG	3 cut(s)	848, 1659, 2606
SmlI	CTYRAG	1 cut(s)	2791
SmoI	CTYRAG	1 cut(s)	2791
SpeI	ACTAGT	1 cut(s)	674
SphI	GCATGC	1 cut(s)	1777
Sse9I	AATT	8 cut(s)	149, 170, 334, 471, 2034, 2325, 2872, 3101
SseBI	AGGCCT	1 cut(s)	2638
SsiI	CCGC	8 cut(s)	836, 1442, 1696, 1853, 2063, 2074, 2302, 2429
SspDI	GGCGCC	1 cut(s)	176
SspI	AATATT	1 cut(s)	2130
SstI	GAGCTC	4 cut(s)	257, 614, 749, 903
StuI	AGGCCT	1 cut(s)	2638
StyD4I	CCNGG	5 cut(s)	1789, 2065, 2146, 2263, 2655
StyI	CCWWGG	4 cut(s)	731, 2639, 2756, 3110
TaaI	ACNGT	8 cut(s)	697, 774, 1377, 1546, 1867, 2279, 2362, 2460
TaiI	ACGT	2 cut(s)	144, 3169
TasI	AATT	8 cut(s)	149, 170, 334, 471, 2034, 2325, 2872, 3101
TatI	WGTACW	5 cut(s)	330, 1554, 2460, 2976, 3057
TauI	GCSGC	3 cut(s)	1445, 1856, 2431
TfiI	GAWTC	8 cut(s)	979, 1076, 1096, 1249, 1318, 1951, 2192, 2611
Tru1I	TTAA	2 cut(s)	975, 1302
Tru9I	TTAA	2 cut(s)	975, 1302
TseFI	GTSAC	3 cut(s)	990, 1898, 3070
TseI	GCWGC	7 cut(s)	79, 856, 1004, 1007, 1057, 1426, 3125
Tsp45I	GTSAC	3 cut(s)	990, 1898, 3070
TspGWI	ACGGA	4 cut(s)	142, 191, 1092, 1673
Van91I	CCANNNNNTGG	1 cut(s)	3207
VpaK11BI	GGWCC	4 cut(s)	875, 1090, 1535, 2569
XapI	RAATTY	3 cut(s)	149, 170, 2034
XceI	RCATGY	4 cut(s)	1186, 1777, 1947, 3064
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3094
XmaJI	CCTAGG	1 cut(s)	2639
XmiI	GTMKAC	4 cut(s)	1278, 2357, 2721, 3220

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.