Rorug06G0256600
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005581
Physical Location & Seq
Forward (+)
40341815 .. 40347051
5237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0256600.1

Sequence Viewer

Length: 3471 bp
ATGGCTCTTGTAAGATCAGCTCATCCAGAACCCTCCTCTTTCCCAAATGCTTCTCCTTCAATATCTAGTCATGATGTGTTCTTGAGTTTCCGAGGTGAAGACACTCGGAAGACTTTCACTGACCACCTCTATACAGCCTTAATGAATGCAGGCTTTCGTACGTTTCGAGATGACAATGATCTTCAAAGAGGAAAAAATATTAAGCCGGAACTGGAGAAAGCCATCGAGCAGTCCCGAAGTTCTGTGATTGTGTTCTCCAAAGACTATGCATCTTCTGGTTGGTGCCTTGACGAGCTTTTGATGATCATGAAACGGAGGAGGACAGCTGATCACATAGTTTTACCCGTCTTCTATGATGTCGATCCATCCCATGTGAGGAAGCAAACAGGAAGTCTTGCGAAAGCATTTGCTAGACACGTAAAAAATCAATCCTTGAAGGACAAGGTGAAGCGATGGAGGGAAGCACTTACAGAAGCTGCAGATCTATCAGGAATGGTCTTGCAAAATGAAGCTGACGGGCATGAGTCAAAGTTCATAAAGAAAGTTGTCAAAGTAATTGAAGATAGACTGAGGCGCACGCCCTTAAGTGTTGACAGACACTTGGTTGGAATCAAGTCTCAAGTTGAAGAGATCAACTTGTGGTTAGATGATGGATCAACTGATAATTTCATACTTCTAATCTATGGAATGCGAGGAATAGGGAAGTCAACCATAGCAAAATTTGTTTATAATCTGAACCGTCACAGATTTGAGAGATGCAGTTTCCTTGAAGATATCAGAGAATTTTCAAAACGATCTAATGGCTTGGTTGATTTGCAGAAGCAACTTCTTTATGATATTCTGAGTGGCAAGAAAGTCAAAATACAGGGTATTAGTGAGGGGATAGCTAAGATTGAAGATGCTGTCAGCTCTCGAAGGGTTTTTCTTGTTCTTGATGATGTGGATCATGTGGACCACTTAGATGCATTACTTAGAATGCAAAACCGGTTCTATCCAGGAAGTAAAATTATCATAACAACTAGCTGTGCAGGACTGGTAGAAGCTCATCGACAATTTGTTAAGGTGCATGAAGTTAATACTTTGGGGTTCTATGAATCTTTGGAACTCTTTAGTTGGCATGCTTTTGGACAGAGCCATCCCATTGAAAGTTACATGGATCTTTCCCAAAGGATAGTTGATCACTGTGGACAACTTCCACTGGCTCTTAAAGTTTTAGGTTCTTCTTTATCAGGGAAGAGATTAGGTTTTTGGAAAAGTTATGTAAATAAATTGGAAGTAATTCCACATGGTGATATCTTGAAGACACTCAAAATAAGCTATGAATCCTTACAAGATGATCATGACAGAAACTTATTCCTTCATATTGCATGCTTTTTCATCGGAATGGAGAAAGATGTCATTGTTACAATTTTAGATGACTCTGATATCTTCCCGGAAGTTGGCATTCAAAATCTCATTGATAGATGTTTGGTAACAATTGAATACAACGAGGTGCAAATGCATAACTTGATTCGCGACATGGGAAGAGAAATTGCTCGTCTAGAATCAAAGGAGCCAAGAAAACACAGAAGATTATGGCATCATAAGGATTCTATCAAAGTTCTGACAGAAAATAATGGTACCGAAGAAATTGAAGGTATTGTTGTCAATATGCTACTGCACCCTACATATACTTCCCCTTCAAGAAATGCAAGTAAGTATATCCCTTCACTAAAAATCCTAAATCTCAGCCACTCTCATGACTTAACTGAAACCCCTGACTTCTCGTCGATTCCTAATCTTGAGAGGCTGAATCTTAGAGACTGTGTAAGCTTGATTGATGTCCGTGAGTCTATTGGAAGCCTAGAGAGACTTGTTTATTTGAATGTAGAAGATTGCACAAGTATTAGGAAGCTACCCAAGAACATTTCTTTGCTACCTTTACTGGAAGTACTAGTCATTGCTGGCTGTTCAAGTCTTGATGAGTTTCCAGTGGACATGAGGAAGATGGAATCTCTCAAAGAATTTCAGGCAGATGGAGTTCCAATACATCGGTTACTAGTCACTACCACCAGGGAGGTCGAACTGTGCTCAAGTCCGACTCAAGGACTTTCTTGGGCTTCTTATCTTCCAAGGAATTTAGTAAACTTGAGTCTAAGAAACTGCAATCTTTTGGATGATGATTTTCCTAGTGATGTTGCTAATCTATTGTCATTGCAAAACTTGGATCTCAGCGGGAACCCAATTTCTAAGCTACCGGATTGCATAAGAGGTGTTACTGGCCTCCAAGAACTCTCTTTCCAAGATTGTACAAAGCTCAAATCACTTCTAAGGCTACCAGATTCACTTATTTTCATTATGGGCGGGTGCACATCATTGGAAAAAGTAACATTTCAATCTCTTTCTCACACTGATTGTGATTGGTGGTCCAACCATAACCTTGTTGAGTTACAGGGTGACTTCAAGATGGAACCCATTGAAAGAGTTGATAAAGAAATGCTAAAACTTTTGAGTCTAGGCAACCTGGAAACCTTGGAAAACATTTTGATGGACAATACTGGATCTGTTTCTCGATGGAAGGGAACACATCCCGTCCAGGGACTGTACGAAAATGGTATATTCAGCACATTCCTTCCTGGAGACGAAGTTCCAGGCCAGTTCAGCCATAGAAGCAGAGGGTCCTCTGTATCCTTTACTGTGCCTTTACTTCCCAATCTCAATATACGAGGGCTGAATGTCTTCTCGGTCATAGCACAATCCAACAACAATGATTCTGATCCTCTGATCAATAGTGTCAATATTGACGGTTTTCATCCGGCAATTACCGTCGCGATTAATAAGAGCAAGGGGCTGAAGTGGATCCATGGTCCAAGCTTCTCTGGCGTTCCGGGTGAAGGAAAAGATGTGATATGGTTAAGCCACTGGAAGTTTGGGAATCAACTTACATGTGGTGATGAGGTGACTATTTTAATATATACGAAATCTGATTTCAAGGTAAAGGAGTGTGGCATCCAGCTTGTTTACTACGATGAGAAAGATCAAGAGAAGATAATTGGTACCCAACTTGGGGCTTCCACCGTGGACGATCTTTGTTTCCTCCGAGCTGGAGTTCGGTCAACAATACCCTGTCATCCGGGCAAAGGAATAACCTTCATGGGCCCCTGCCTCTTCTACGAAGGAATAGAAGATACTGACCTCATTAGGGATATCAATGAAAAATCAGAGAAAGAAGAAGGGCAAGAGGGTGACCTCGTCCTCACTCTTGCAGCACAAAGTGGCAGCAATACCAATAAACGAGGTTGCTGGGGTGGGAAGGAGAAGAGAAGGGATTCAAGAATCAAGATTGTGGTGGGCTTCGGGGGAAGGGTTTGGGTTTGTTTTCGGAGTGGTGGATTCGAAAATTTTGATCGAGTTTGGGGGATAATTTTCAGCAGGAAAAAGGTTCTGTTGTGTGTCTTGGACTATTTGGTTTTGGTTAAAGGAGTTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

1156

Amino Acids

130.54

Weight (kDa)

6.37

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 24 - 196 2.4e-53 TIR domain
TIR_2 PF13676 26 - 122 9.1e-18 TIR domain
NB-ARC PF00931 205 - 376 6.4e-16 NB-ARC domain
AAA_14 PF13173 223 - 348 8.7e-06 AAA domain
WHD_ROQ1 PF23282 447 - 515 2.3e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 581 - 672 3.4e-06 Leucine-rich repeat region
LRR_14 PF23598 710 - 859 3.2e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 729
Acc65I GGTACC 2 cut(s) 1619, 3035
AccB1I GGYRCC 3 cut(s) 282, 1619, 3035
AccII CGCG 2 cut(s) 1515, 2810
AciI CCGC 2 cut(s) 2214, 2343
AclWI GGATC 9 cut(s) 356, 661, 951, 1164, 2214, 2548, 2750, 2833, 2846
AcsI RAATTY 5 cut(s) 719, 782, 2003, 2116, 3379
AcuI CTGAAG 1 cut(s) 2852
AdeI CACNNNGTG 2 cut(s) 1289, 3254
AfaI GTAC 6 cut(s) 160, 1621, 1932, 2290, 2585, 3037
AfiI CCNNNNNNNGG 9 cut(s) 375, 1439, 2084, 2576, 2873, 3045, 3046, 3119, 3181
AflII CTTAAG 1 cut(s) 583
AflIII ACRYGT 2 cut(s) 415, 2924
AgeI ACCGGT 1 cut(s) 984
AhdI GACNNNNNGTC 1 cut(s) 1765
AhlI ACTAGT 2 cut(s) 1933, 2038
AjnI CCWGG 6 cut(s) 994, 2051, 2502, 2574, 2614, 2629
AloI GAACNNNNNNTCC 4 cut(s) 2262, 2294, 2610, 2642
Alw21I GWGCWC 2 cut(s) 2072, 2351
Alw26I GTCTC 4 cut(s) 621, 1794, 1843, 2613
Alw44I GTGCAC 1 cut(s) 2347
AlwI GGATC 9 cut(s) 356, 661, 951, 1164, 2214, 2548, 2750, 2833, 2846
AlwNI CAGNNNCTG 2 cut(s) 476, 2581
AoxI GGCC 3 cut(s) 2260, 2632, 3136
ApaI GGGCCC 1 cut(s) 3140
ApaLI GTGCAC 1 cut(s) 2347
ApeKI GCWGC 3 cut(s) 476, 3245, 3258
ApoI RAATTY 5 cut(s) 719, 782, 2003, 2116, 3379
ArsI GACNNNNNNTTYG 2 cut(s) 376, 408
AseI ATTAAT 1 cut(s) 2814
AsiGI ACCGGT 1 cut(s) 984
Asp700I GAANNNNTTC 4 cut(s) 113, 1278, 2717, 3307
Asp718I GGTACC 2 cut(s) 1619, 3035
AspLEI GCGC 1 cut(s) 576
AspS9I GGNCC 6 cut(s) 952, 2406, 2658, 2846, 3136, 3137
AsuC2I CCSGG 3 cut(s) 1433, 2868, 3114
AsuHPI GGTGA 8 cut(s) 107, 457, 1301, 2447, 2882, 2942, 2950, 3236
AsuII TTCGAA 1 cut(s) 3375
AvaII GGWCC 4 cut(s) 952, 2406, 2658, 2846
BaeGI GKGCMC 2 cut(s) 2351, 3140
BamHI GGATCC 1 cut(s) 2838
BanI GGYRCC 3 cut(s) 282, 1619, 3035
BanII GRGCYC 1 cut(s) 3140
BbsI GAAGAC 5 cut(s) 105, 116, 340, 1307, 2710
Bbv12I GWGCWC 2 cut(s) 2072, 2351
BbvI GCAGC 3 cut(s) 463, 3257, 3270
BciT130I CCWGG 6 cut(s) 996, 2053, 2504, 2576, 2616, 2631
BciVI GTATCC 1 cut(s) 2677
BclI TGATCA 5 cut(s) 303, 328, 1177, 1336, 2763
BcnI CCSGG 3 cut(s) 1433, 2868, 3114
BcoDI GTCTC 4 cut(s) 621, 1794, 1843, 2613
BcuI ACTAGT 2 cut(s) 1933, 2038
BfaI CTAG 9 cut(s) 66, 411, 1020, 1541, 1844, 1934, 2039, 2169, 2495
BfmI CTRYAG 1 cut(s) 477
BfrI CTTAAG 1 cut(s) 583
BfuI GTATCC 1 cut(s) 2677
BglII AGATCT 1 cut(s) 481
BisI GCNGC 3 cut(s) 477, 3246, 3259
BlsI GCNGC 3 cut(s) 478, 3247, 3260
BmcAI AGTACT 1 cut(s) 1932
Bme1390I CCNGG 9 cut(s) 996, 1433, 2053, 2504, 2576, 2616, 2631, 2868, 3114
Bme18I GGWCC 4 cut(s) 952, 2406, 2658, 2846
BmeRI GACNNNNNGTC 1 cut(s) 1765
BmgT120I GGNCC 6 cut(s) 952, 2406, 2658, 2846, 3136, 3137
BmrFI CCNGG 9 cut(s) 996, 1433, 2053, 2504, 2576, 2616, 2631, 2868, 3114
BmsI GCATC 6 cut(s) 278, 746, 889, 952, 1588, 2997
BpiI GAAGAC 5 cut(s) 105, 116, 340, 1307, 2710
BpmI CTGGAG 3 cut(s) 233, 2637, 3105
Bpu14I TTCGAA 1 cut(s) 3375
BpuEI CTTGAG 6 cut(s) 103, 603, 1802, 2056, 2067, 2149
BpuMI CCSGG 3 cut(s) 1433, 2868, 3114
BsaAI YACGTR 1 cut(s) 418
BsaBI GATNNNNATC 3 cut(s) 360, 942, 2754
BsaJI CCNNGG 7 cut(s) 91, 2052, 2111, 2511, 2575, 2842, 3057
BsaWI WCCGGW 2 cut(s) 984, 2236
BsaXI ACNNNNNCTCC 2 cut(s) 2610, 2640
Bsc4I CCNNNNNNNGG 9 cut(s) 375, 1439, 2084, 2576, 2873, 3045, 3046, 3119, 3181
Bse118I RCCGGY 1 cut(s) 984
Bse1I ACTGG 9 cut(s) 216, 1038, 1203, 1929, 1970, 2263, 2542, 2635, 2906
Bse3DI GCAATG 2 cut(s) 1938, 2192
Bse8I GATNNNNATC 3 cut(s) 360, 942, 2754
BseBI CCWGG 6 cut(s) 996, 2053, 2504, 2576, 2616, 2631
BseDI CCNNGG 7 cut(s) 91, 2052, 2111, 2511, 2575, 2842, 3057
BseGI GGATG 8 cut(s) 22, 365, 1135, 2161, 2566, 2791, 2988, 3109
BseJI GATNNNNATC 3 cut(s) 360, 942, 2754
BseLI CCNNNNNNNGG 9 cut(s) 375, 1439, 2084, 2576, 2873, 3045, 3046, 3119, 3181
BseMI GCAATG 2 cut(s) 1938, 2192
BseMII CTCAG 4 cut(s) 560, 833, 1741, 2224
BseNI ACTGG 9 cut(s) 216, 1038, 1203, 1929, 1970, 2263, 2542, 2635, 2906
BseRI GAGGAG 2 cut(s) 25, 331
BseSI GKGCMC 2 cut(s) 2351, 3140
BseXI GCAGC 3 cut(s) 463, 3257, 3270
BseYI CCCAGC 1 cut(s) 3282
BsgI GTGCAG 2 cut(s) 1047, 1643
Bsh1236I CGCG 2 cut(s) 1515, 2810
BshFI GGCC 3 cut(s) 2262, 2634, 3138
BshNI GGYRCC 3 cut(s) 282, 1619, 3035
BshTI ACCGGT 1 cut(s) 984
BsiHKAI GWGCWC 2 cut(s) 2072, 2351
BsiSI CCGG 7 cut(s) 206, 985, 1433, 2237, 2795, 2867, 3113
BsiWI CGTACG 1 cut(s) 158
BslFI GGGAC 2 cut(s) 217, 2592
BslI CCNNNNNNNGG 9 cut(s) 375, 1439, 2084, 2576, 2873, 3045, 3046, 3119, 3181
BsmAI GTCTC 4 cut(s) 621, 1794, 1843, 2613
BsmBI CGTCTC 1 cut(s) 2613
BsmFI GGGAC 2 cut(s) 217, 2592
BsmI GAATGC 4 cut(s) 151, 693, 981, 1443
BsnI GGCC 3 cut(s) 2262, 2634, 3138
Bsp119I TTCGAA 1 cut(s) 3375
Bsp120I GGGCCC 1 cut(s) 3136
Bsp1286I GDGCHC 3 cut(s) 2072, 2351, 3140
Bsp1407I TGTACA 1 cut(s) 2288
Bsp19I CCATGG 1 cut(s) 2842
Bsp68I TCGCGA 2 cut(s) 1515, 2810
BspACI CCGC 2 cut(s) 2214, 2343
BspANI GGCC 3 cut(s) 2262, 2634, 3138
BspCNI CTCAG 4 cut(s) 561, 834, 1740, 2223
BspFNI CGCG 2 cut(s) 1515, 2810
BspHI TCATGA 4 cut(s) 70, 306, 1339, 1738
BspMAI CTGCAG 1 cut(s) 481
BspPI GGATC 9 cut(s) 356, 661, 951, 1164, 2214, 2548, 2750, 2833, 2846
BspT104I TTCGAA 1 cut(s) 3375
BspT107I GGYRCC 3 cut(s) 282, 1619, 3035
BspTI CTTAAG 1 cut(s) 583
BsrDI GCAATG 2 cut(s) 1938, 2192
BsrFI RCCGGY 1 cut(s) 984
BsrGI TGTACA 1 cut(s) 2288
BsrI ACTGG 9 cut(s) 216, 1038, 1203, 1929, 1970, 2263, 2542, 2635, 2906
BssAI RCCGGY 1 cut(s) 984
BssECI CCNNGG 7 cut(s) 91, 2052, 2111, 2511, 2575, 2842, 3057
BssT1I CCWWGG 3 cut(s) 2111, 2511, 2842
Bst2UI CCWGG 6 cut(s) 996, 2053, 2504, 2576, 2616, 2631
Bst4CI ACNGT 9 cut(s) 740, 1184, 1805, 2067, 2583, 2677, 2786, 2806, 3058
Bst6I CTCTTC 5 cut(s) 621, 1229, 1519, 3152, 3293
BstAFI CTTAAG 1 cut(s) 583
BstAUI TGTACA 1 cut(s) 2288
BstBAI YACGTR 1 cut(s) 418
BstBI TTCGAA 1 cut(s) 3375
BstC8I GCNNGC 5 cut(s) 151, 578, 1119, 1369, 1945
BstDSI CCRYGG 2 cut(s) 2842, 3057
BstEII GGTNACC 1 cut(s) 3224
BstF5I GGATG 8 cut(s) 22, 365, 1135, 2161, 2566, 2791, 2988, 3109
BstFNI CGCG 2 cut(s) 1515, 2810
BstHHI GCGC 1 cut(s) 576
BstMAI GTCTC 4 cut(s) 621, 1794, 1843, 2613
BstMWI GCNNNNNNNGC 3 cut(s) 2640, 2649, 2859
BstNI CCWGG 6 cut(s) 996, 2053, 2504, 2576, 2616, 2631
BstNSI RCATGY 3 cut(s) 1121, 1371, 2928
BstPI GGTNACC 1 cut(s) 3224
BstSCI CCNGG 9 cut(s) 994, 1431, 2051, 2502, 2574, 2614, 2629, 2866, 3112
BstSFI CTRYAG 1 cut(s) 477
BstSLI GKGCMC 2 cut(s) 2351, 3140
BstUI CGCG 2 cut(s) 1515, 2810
BstV1I GCAGC 3 cut(s) 463, 3257, 3270
BstV2I GAAGAC 5 cut(s) 105, 116, 340, 1307, 2710
BstX2I RGATCY 5 cut(s) 481, 1156, 2206, 2540, 2838
BstYI RGATCY 5 cut(s) 481, 1156, 2206, 2540, 2838
BsuI GTATCC 1 cut(s) 2677
BsuRI GGCC 3 cut(s) 2262, 2634, 3138
BtgI CCRYGG 2 cut(s) 2842, 3057
BtgZI GCGATG 1 cut(s) 466
BtsCI GGATG 8 cut(s) 22, 365, 1135, 2161, 2566, 2791, 2988, 3109
BtsIMutI CAGTG 6 cut(s) 117, 1180, 1196, 1977, 2388, 2899
BtuMI TCGCGA 2 cut(s) 1515, 2810
Cac8I GCNNGC 5 cut(s) 151, 578, 1119, 1369, 1945
CaiI CAGNNNCTG 2 cut(s) 476, 2581
CciI TCATGA 4 cut(s) 70, 306, 1339, 1738
CfoI GCGC 1 cut(s) 576
Cfr10I RCCGGY 1 cut(s) 984
Cfr13I GGNCC 6 cut(s) 952, 2406, 2658, 2846, 3136, 3137
Csp6I GTAC 6 cut(s) 159, 1620, 1931, 2289, 2584, 3036
CspAI ACCGGT 1 cut(s) 984
CviQI GTAC 6 cut(s) 159, 1620, 1931, 2289, 2584, 3036
DraIII CACNNNGTG 2 cut(s) 1289, 3254
DriI GACNNNNNGTC 1 cut(s) 1765
Eam1104I CTCTTC 5 cut(s) 621, 1229, 1519, 3152, 3293
Eam1105I GACNNNNNGTC 1 cut(s) 1765
EarI CTCTTC 5 cut(s) 621, 1229, 1519, 3152, 3293
Eco130I CCWWGG 3 cut(s) 2111, 2511, 2842
Eco24I GRGCYC 1 cut(s) 3140
Eco32I GATATC 4 cut(s) 775, 1294, 1426, 3187
Eco47I GGWCC 4 cut(s) 952, 2406, 2658, 2846
Eco57I CTGAAG 1 cut(s) 2852
Eco91I GGTNACC 1 cut(s) 3224
EcoO109I RGGNCCY 2 cut(s) 2658, 3137
EcoO65I GGTNACC 1 cut(s) 3224
EcoRII CCWGG 6 cut(s) 994, 2051, 2502, 2574, 2614, 2629
EcoRV GATATC 4 cut(s) 775, 1294, 1426, 3187
EcoT14I CCWWGG 3 cut(s) 2111, 2511, 2842
EcoT22I ATGCAT 3 cut(s) 271, 967, 1503
EcoT38I GRGCYC 1 cut(s) 3140
ErhI CCWWGG 3 cut(s) 2111, 2511, 2842
Esp3I CGTCTC 1 cut(s) 2613
FaqI GGGAC 2 cut(s) 217, 2592
FauI CCCGC 2 cut(s) 2207, 2336
FbaI TGATCA 5 cut(s) 303, 328, 1177, 1336, 2763
Fnu4HI GCNGC 3 cut(s) 477, 3246, 3259
FokI GGATG 8 cut(s) 9, 352, 1122, 2168, 2553, 2778, 2975, 3096
FriOI GRGCYC 1 cut(s) 3140
Fsp4HI GCNGC 3 cut(s) 477, 3246, 3259
FspBI CTAG 9 cut(s) 66, 411, 1020, 1541, 1844, 1934, 2039, 2169, 2495
GlaI GCGC 1 cut(s) 575
GluI GCNGC 3 cut(s) 477, 3246, 3259
GsaI CCCAGC 1 cut(s) 3286
GsuI CTGGAG 3 cut(s) 233, 2637, 3105
HaeIII GGCC 3 cut(s) 2262, 2634, 3138
HapII CCGG 7 cut(s) 206, 985, 1433, 2237, 2795, 2867, 3113
HhaI GCGC 1 cut(s) 576
Hin6I GCGC 1 cut(s) 574
HinP1I GCGC 1 cut(s) 574
HincII GTYRAC 3 cut(s) 592, 708, 3096
HindII GTYRAC 3 cut(s) 592, 708, 3096
HindIII AAGCTT 2 cut(s) 1810, 2851
HpaII CCGG 7 cut(s) 206, 985, 1433, 2237, 2795, 2867, 3113
HphI GGTGA 8 cut(s) 107, 457, 1301, 2447, 2882, 2942, 2950, 3236
Hpy99I CGWCG 2 cut(s) 1771, 2810
HpyCH4III ACNGT 9 cut(s) 740, 1184, 1805, 2067, 2583, 2677, 2786, 2806, 3058
HpyCH4IV ACGT 2 cut(s) 161, 417
HpyF10VI GCNNNNNNNGC 3 cut(s) 2640, 2649, 2859
HpySE526I ACGT 2 cut(s) 161, 417
HspAI GCGC 1 cut(s) 574
KpnI GGTACC 2 cut(s) 1623, 3039
Ksp22I TGATCA 5 cut(s) 303, 328, 1177, 1336, 2763
LmnI GCTCC 1 cut(s) 1552
Lsp1109I GCAGC 3 cut(s) 463, 3257, 3270
LweI GCATC 6 cut(s) 278, 746, 889, 952, 1588, 2997
MaeI CTAG 9 cut(s) 66, 411, 1020, 1541, 1844, 1934, 2039, 2169, 2495
MaeII ACGT 2 cut(s) 161, 417
MfeI CAATTG 1 cut(s) 1476
MflI RGATCY 5 cut(s) 481, 1156, 2206, 2540, 2838
MhlI GDGCHC 3 cut(s) 2072, 2351, 3140
MlyI GAGTC 6 cut(s) 533, 1412, 1838, 2074, 2140, 2500
MmeI TCCRAC 4 cut(s) 586, 2102, 2433, 2763
Mph1103I ATGCAT 3 cut(s) 271, 967, 1503
MroXI GAANNNNTTC 4 cut(s) 113, 1278, 2717, 3307
MslI CAYNNNNRTG 4 cut(s) 960, 1382, 1737, 2525
MspA1I CMGCKG 2 cut(s) 326, 2214
MspCI CTTAAG 1 cut(s) 583
MspI CCGG 7 cut(s) 206, 985, 1433, 2237, 2795, 2867, 3113
MspR9I CCNGG 9 cut(s) 996, 1433, 2053, 2504, 2576, 2616, 2631, 2868, 3114
MunI CAATTG 1 cut(s) 1476
Mva1269I GAATGC 4 cut(s) 151, 693, 981, 1443
MvaI CCWGG 6 cut(s) 996, 2053, 2504, 2576, 2616, 2631
MvnI CGCG 2 cut(s) 1515, 2810
MwoI GCNNNNNNNGC 3 cut(s) 2640, 2649, 2859
NciI CCSGG 3 cut(s) 1433, 2868, 3114
NcoI CCATGG 1 cut(s) 2842
NmuCI GTSAC 5 cut(s) 740, 2041, 2435, 2938, 3224
NruI TCGCGA 2 cut(s) 1515, 2810
NsiI ATGCAT 3 cut(s) 271, 967, 1503
NspI RCATGY 3 cut(s) 1121, 1371, 2928
NspV TTCGAA 1 cut(s) 3375
PaeI GCATGC 2 cut(s) 1121, 1371
PagI TCATGA 4 cut(s) 70, 306, 1339, 1738
PciI ACATGT 1 cut(s) 2924
PcsI WCGNNNNNNNCGW 1 cut(s) 163
PctI GAATGC 4 cut(s) 151, 693, 981, 1443
PdmI GAANNNNTTC 4 cut(s) 113, 1278, 2717, 3307
Pfl23II CGTACG 1 cut(s) 158
PflFI GACNNNGTC 1 cut(s) 3230
PfoI TCCNGGA 3 cut(s) 994, 1431, 2614
PinAI ACCGGT 1 cut(s) 984
PkrI GCNGC 3 cut(s) 478, 3247, 3260
PleI GAGTC 6 cut(s) 532, 1412, 1837, 2074, 2139, 2499
PpsI GAGTC 6 cut(s) 532, 1412, 1837, 2074, 2139, 2499
Ppu21I YACGTR 1 cut(s) 418
PpuMI RGGWCCY 1 cut(s) 2658
PscI ACATGT 1 cut(s) 2924
PshBI ATTAAT 1 cut(s) 2814
PsiI TTATAA 1 cut(s) 729
Psp5II RGGWCCY 1 cut(s) 2658
Psp6I CCWGG 6 cut(s) 994, 2051, 2502, 2574, 2614, 2629
PspEI GGTNACC 1 cut(s) 3224
PspFI CCCAGC 1 cut(s) 3282
PspGI CCWGG 6 cut(s) 994, 2051, 2502, 2574, 2614, 2629
PspLI CGTACG 1 cut(s) 158
PspOMI GGGCCC 1 cut(s) 3136
PspPI GGNCC 6 cut(s) 952, 2406, 2658, 2846, 3136, 3137
PspPPI RGGWCCY 1 cut(s) 2658
PstI CTGCAG 1 cut(s) 481
PstNI CAGNNNCTG 2 cut(s) 476, 2581
PsuI RGATCY 5 cut(s) 481, 1156, 2206, 2540, 2838
PsyI GACNNNGTC 1 cut(s) 3230
PvuII CAGCTG 1 cut(s) 326
RruI TCGCGA 2 cut(s) 1515, 2810
RsaI GTAC 6 cut(s) 160, 1621, 1932, 2290, 2585, 3037
RsaNI GTAC 6 cut(s) 159, 1620, 1931, 2289, 2584, 3036
RseI CAYNNNNRTG 4 cut(s) 960, 1382, 1737, 2525
SatI GCNGC 3 cut(s) 477, 3246, 3259
Sau96I GGNCC 6 cut(s) 952, 2406, 2658, 2846, 3136, 3137
ScaI AGTACT 1 cut(s) 1932
SchI GAGTC 6 cut(s) 533, 1412, 1838, 2074, 2140, 2500
ScrFI CCNGG 9 cut(s) 996, 1433, 2053, 2504, 2576, 2616, 2631, 2868, 3114
SduI GDGCHC 3 cut(s) 2072, 2351, 3140
SfaNI GCATC 6 cut(s) 278, 746, 889, 952, 1588, 2997
SfcI CTRYAG 1 cut(s) 477
SfuI TTCGAA 1 cut(s) 3375
SinI GGWCC 4 cut(s) 952, 2406, 2658, 2846
SmiMI CAYNNNNRTG 4 cut(s) 960, 1382, 1737, 2525
SmlI CTYRAG 7 cut(s) 82, 583, 618, 1781, 2071, 2082, 2128
SmoI CTYRAG 7 cut(s) 82, 583, 618, 1781, 2071, 2082, 2128
SpeI ACTAGT 2 cut(s) 1933, 2038
SphI GCATGC 2 cut(s) 1121, 1371
SsiI CCGC 2 cut(s) 2214, 2343
SspI AATATT 2 cut(s) 199, 2779
SspMI CTAG 9 cut(s) 66, 411, 1020, 1541, 1844, 1934, 2039, 2169, 2495
StyD4I CCNGG 9 cut(s) 994, 1431, 2051, 2502, 2574, 2614, 2629, 2866, 3112
StyI CCWWGG 3 cut(s) 2111, 2511, 2842
TaaI ACNGT 9 cut(s) 740, 1184, 1805, 2067, 2583, 2677, 2786, 2806, 3058
TaiI ACGT 2 cut(s) 164, 420
TaqII GACCGA 2 cut(s) 2713, 3081
TatI WGTACW 2 cut(s) 1930, 2288
TscAI CASTG 6 cut(s) 124, 1187, 1203, 1977, 2395, 2906
TseFI GTSAC 5 cut(s) 740, 2041, 2435, 2938, 3224
TseI GCWGC 3 cut(s) 476, 3245, 3258
Tsp45I GTSAC 5 cut(s) 740, 2041, 2435, 2938, 3224
TspGWI ACGGA 2 cut(s) 328, 1814
TspRI CASTG 6 cut(s) 124, 1187, 1203, 1977, 2395, 2906
Tth111I GACNNNGTC 1 cut(s) 3230
Vha464I CTTAAG 1 cut(s) 583
VneI GTGCAC 1 cut(s) 2347
VpaK11BI GGWCC 4 cut(s) 952, 2406, 2658, 2846
VspI ATTAAT 1 cut(s) 2814
XapI RAATTY 5 cut(s) 719, 782, 2003, 2116, 3379
XbaI TCTAGA 1 cut(s) 1540
XceI RCATGY 3 cut(s) 1121, 1371, 2928
XcmI CCANNNNNNNNNTGG 1 cut(s) 2906
XmnI GAANNNNTTC 4 cut(s) 113, 1278, 2717, 3307
XspI CTAG 9 cut(s) 66, 411, 1020, 1541, 1844, 1934, 2039, 2169, 2495
ZrmI AGTACT 1 cut(s) 1932
Zsp2I ATGCAT 3 cut(s) 271, 967, 1503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.