Rh6DG370000
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005581
Physical Location & Seq
Reverse (-)
56575905 .. 56577507
1603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG370000.1

Sequence Viewer

Length: 480 bp
ATGTCTCATCCACAACAAGAAGAAGAAGAGGAACTGGGCAAACAAGCCATACGTTTAGCCAATGTCGTGATCCTTCCGATGGTTCTGAAGTCAGCTCTTGAACTCAACGTCATAGACATTATCTGGGGTGCAGGCGACGGCGAGTCACTCTCACCGTCCGACATTGCGGCTCAGCTCCCCACCAAGAACTCCAACGCCCCGGCTGTTCTGGACCGGATGCTTAGGCTCTTGGCCAGCCACTCTATACTCAAATGCTCGGCCCGGACCGGATCAGATGGGCAGGTAGAGAGGTTGTATAGTGCAGGACCCATATGCAAATTCCTTGTCAAGGATCAAAATGGAGGAACCGGGTCCGTTGGCCCTCTGTTTCTCTTGCACCATGATAAGTTGTTTGCTCTGCTTACAACAGCTGGGTCATGGAGTTCCACAAATGAGCAAATCCTTGCATCGGTGCAGTCCGAGAACGTGCTTGTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

159

Amino Acids

17.03

Weight (kDa)

5.28

Isoelectric Point (pI)

37.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 18 - 111 2.1e-19 O-methyltransferase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 271
AciI CCGC 1 cut(s) 167
AclWI GGATC 3 cut(s) 64, 277, 339
AcoI YGGCCR 1 cut(s) 231
AcsI RAATTY 1 cut(s) 317
AcuI CTGAAG 1 cut(s) 107
AfiI CCNNNNNNNGG 3 cut(s) 79, 328, 448
AgsI TTSAA 1 cut(s) 101
AhdI GACNNNNNGTC 1 cut(s) 142
AluBI AGCT 3 cut(s) 95, 175, 410
AluI AGCT 3 cut(s) 95, 175, 410
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 3 cut(s) 64, 277, 339
AoxI GGCC 3 cut(s) 231, 258, 358
ApoI RAATTY 1 cut(s) 317
ArsI GACNNNNNNTTYG 2 cut(s) 309, 341
AspS9I GGNCC 6 cut(s) 211, 259, 264, 305, 351, 359
AsuC2I CCSGG 3 cut(s) 200, 262, 349
AsuHPI GGTGA 1 cut(s) 144
AvaII GGWCC 4 cut(s) 211, 264, 305, 351
BalI TGGCCA 1 cut(s) 233
BccI CCATC 2 cut(s) 73, 269
BceAI ACGGC 1 cut(s) 154
BcnI CCSGG 3 cut(s) 200, 262, 349
BcoDI GTCTC 1 cut(s) 9
BfuAI ACCTGC 1 cut(s) 271
BisI GCNGC 1 cut(s) 168
BlpI GCTNAGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 169
Bme1390I CCNGG 3 cut(s) 200, 262, 349
Bme18I GGWCC 4 cut(s) 211, 264, 305, 351
BmeRI GACNNNNNGTC 1 cut(s) 142
BmgT120I GGNCC 6 cut(s) 211, 259, 264, 305, 351, 359
BmiI GGNNCC 3 cut(s) 307, 346, 352
BmrFI CCNGG 3 cut(s) 200, 262, 349
BmrI ACTGGG 1 cut(s) 44
BmsI GCATC 2 cut(s) 207, 455
BmuI ACTGGG 1 cut(s) 44
BplI GAGNNNNNCTC 2 cut(s) 134, 166
Bpu10I CCTNAGC 1 cut(s) 221
Bpu1102I GCTNAGC 1 cut(s) 171
BpuMI CCSGG 3 cut(s) 200, 262, 349
BsaJI CCNNGG 1 cut(s) 198
BsaWI WCCGGW 2 cut(s) 213, 266
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 328, 448
Bse1I ACTGG 1 cut(s) 39
Bse3DI GCAATG 1 cut(s) 162
BseDI CCNNGG 1 cut(s) 198
BseGI GGATG 2 cut(s) 7, 222
BseLI CCNNNNNNNGG 3 cut(s) 79, 328, 448
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 1 cut(s) 185
BseNI ACTGG 1 cut(s) 39
BseYI CCCAGC 1 cut(s) 410
BsgI GTGCAG 3 cut(s) 150, 321, 473
BshFI GGCC 3 cut(s) 233, 260, 360
BsiSI CCGG 5 cut(s) 200, 214, 262, 267, 348
BslI CCNNNNNNNGG 3 cut(s) 79, 328, 448
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 3 cut(s) 233, 260, 360
Bsp143I GATC 3 cut(s) 69, 269, 331
Bsp1720I GCTNAGC 1 cut(s) 171
BspACI CCGC 1 cut(s) 167
BspANI GGCC 3 cut(s) 233, 260, 360
BspCNI CTCAG 1 cut(s) 184
BspLI GGNNCC 3 cut(s) 307, 346, 352
BspMI ACCTGC 1 cut(s) 271
BspPI GGATC 3 cut(s) 64, 277, 339
BsrDI GCAATG 1 cut(s) 162
BsrI ACTGG 1 cut(s) 39
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 3 cut(s) 69, 269, 331
Bst4CI ACNGT 1 cut(s) 156
Bst6I CTCTTC 1 cut(s) 21
BstC8I GCNNGC 2 cut(s) 133, 235
BstDEI CTNAG 2 cut(s) 171, 221
BstENI CCTNNNNNAGG 1 cut(s) 326
BstF5I GGATG 2 cut(s) 7, 222
BstKTI GATC 3 cut(s) 72, 272, 334
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 3 cut(s) 69, 269, 331
BstSCI CCNGG 3 cut(s) 198, 260, 347
BsuRI GGCC 3 cut(s) 233, 260, 360
BtsCI GGATG 2 cut(s) 7, 222
BveI ACCTGC 1 cut(s) 271
Cac8I GCNNGC 2 cut(s) 133, 235
Cfr13I GGNCC 6 cut(s) 211, 259, 264, 305, 351, 359
CpoI CGGWCCG 1 cut(s) 264
CspI CGGWCCG 1 cut(s) 264
CviAII CATG 2 cut(s) 380, 417
DdeI CTNAG 2 cut(s) 171, 221
DpnI GATC 3 cut(s) 71, 271, 333
DpnII GATC 3 cut(s) 69, 269, 331
DriI GACNNNNNGTC 1 cut(s) 142
EaeI YGGCCR 1 cut(s) 231
Eam1104I CTCTTC 1 cut(s) 21
Eam1105I GACNNNNNGTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 21
Eco47I GGWCC 4 cut(s) 211, 264, 305, 351
Eco57I CTGAAG 1 cut(s) 107
EcoNI CCTNNNNNAGG 1 cut(s) 326
EcoO109I RGGNCCY 1 cut(s) 305
FaeI CATG 2 cut(s) 383, 420
FaiI YATR 9 cut(s) 50, 113, 245, 297, 311, 313, 381, 418, 476
FatI CATG 2 cut(s) 379, 416
FauNDI CATATG 1 cut(s) 311
Fnu4HI GCNGC 1 cut(s) 168
FokI GGATG 1 cut(s) 229
Fsp4HI GCNGC 1 cut(s) 168
GluI GCNGC 1 cut(s) 168
GsaI CCCAGC 1 cut(s) 414
HaeIII GGCC 3 cut(s) 233, 260, 360
HapII CCGG 5 cut(s) 200, 214, 262, 267, 348
Hin1II CATG 2 cut(s) 383, 420
HinfI GANTC 1 cut(s) 143
HpaII CCGG 5 cut(s) 200, 214, 262, 267, 348
HphI GGTGA 1 cut(s) 144
Hpy188I TCNGA 5 cut(s) 78, 87, 160, 274, 460
Hpy188III TCNNGA 3 cut(s) 67, 98, 209
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 1 cut(s) 83
HpyCH4III ACNGT 1 cut(s) 156
HpyCH4IV ACGT 3 cut(s) 52, 108, 465
HpyCH4V TGCA 6 cut(s) 131, 302, 315, 376, 446, 454
HpyF3I CTNAG 2 cut(s) 171, 221
HpySE526I ACGT 3 cut(s) 52, 108, 465
Hsp92II CATG 2 cut(s) 383, 420
Kzo9I GATC 3 cut(s) 69, 269, 331
LmnI GCTCC 1 cut(s) 180
LweI GCATC 2 cut(s) 207, 455
MaeII ACGT 3 cut(s) 52, 108, 465
MaeIII GTNAC 1 cut(s) 144
MalI GATC 3 cut(s) 71, 271, 333
MboI GATC 3 cut(s) 69, 269, 331
MboII GAAGA 3 cut(s) 32, 35, 38
MlsI TGGCCA 1 cut(s) 233
MluCI AATT 1 cut(s) 317
MluNI TGGCCA 1 cut(s) 233
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 2 cut(s) 183, 216
MnlI CCTC 4 cut(s) 22, 282, 335, 372
Mox20I TGGCCA 1 cut(s) 233
MscI TGGCCA 1 cut(s) 233
Msp20I TGGCCA 1 cut(s) 233
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 5 cut(s) 200, 214, 262, 267, 348
MspR9I CCNGG 3 cut(s) 200, 262, 349
NciI CCSGG 3 cut(s) 200, 262, 349
NdeI CATATG 1 cut(s) 311
NdeII GATC 3 cut(s) 69, 269, 331
NlaIII CATG 2 cut(s) 383, 420
NlaIV GGNNCC 3 cut(s) 307, 346, 352
NmeAIII GCCGAG 1 cut(s) 236
NmuCI GTSAC 1 cut(s) 144
PkrI GCNGC 1 cut(s) 169
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
PpuMI RGGWCCY 1 cut(s) 305
Psp5II RGGWCCY 1 cut(s) 305
PspFI CCCAGC 1 cut(s) 410
PspN4I GGNNCC 3 cut(s) 307, 346, 352
PspPI GGNCC 6 cut(s) 211, 259, 264, 305, 351, 359
PspPPI RGGWCCY 1 cut(s) 305
PvuII CAGCTG 1 cut(s) 410
Rsr2I CGGWCCG 1 cut(s) 264
RsrII CGGWCCG 1 cut(s) 264
SatI GCNGC 1 cut(s) 168
Sau3AI GATC 3 cut(s) 69, 269, 331
Sau96I GGNCC 6 cut(s) 211, 259, 264, 305, 351, 359
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 3 cut(s) 200, 262, 349
SetI ASST 8 cut(s) 55, 97, 111, 177, 285, 293, 412, 468
SfaNI GCATC 2 cut(s) 207, 455
SinI GGWCC 4 cut(s) 211, 264, 305, 351
Sse9I AATT 1 cut(s) 317
SsiI CCGC 1 cut(s) 167
StyD4I CCNGG 3 cut(s) 198, 260, 347
TaaI ACNGT 1 cut(s) 156
TaiI ACGT 3 cut(s) 55, 111, 468
TasI AATT 1 cut(s) 317
TauI GCSGC 1 cut(s) 170
TseFI GTSAC 1 cut(s) 144
Tsp45I GTSAC 1 cut(s) 144
TspGWI ACGGA 1 cut(s) 343
VpaK11BI GGWCC 4 cut(s) 211, 264, 305, 351
XagI CCTNNNNNAGG 1 cut(s) 326
XapI RAATTY 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.