Rh6DG369900
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005581
Physical Location & Seq
Reverse (-)
56569955 .. 56570416
462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG369900.1

Sequence Viewer

Length: 333 bp
ATGGTTCTAAAGTCGGCTCTTGAACTCAACGTCATAGACATTATCTGGGGTGCAGGCGACGGCGCGTCACTCTCACCATCCGACATTGCTGCTCAGCTCCCTACTAAGAACTCCAACGCCCCGGTTGTTCTGGACCGGATGCTTAGGCTCTTGGCCAGCCATTCTATACTCAAATGCTCGGCCCGGACTGGATCAGATGGACCGGTAGAGAGGTTGTATAGTGCAGGACCCATATGCAAATTCCTCGTCAAGGATCAAAATGGAGGAACTGGGGGGTCCGTTGGTCCTCTGTTTCTCTTGCAGAATGACAAGGTTTTCATGGAAAGCTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

110

Amino Acids

11.64

Weight (kDa)

6.54

Isoelectric Point (pI)

22.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 1 - 85 3.4e-17 O-methyltransferase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 65
AclWI GGATC 2 cut(s) 199, 261
AcoI YGGCCR 1 cut(s) 153
AcsI RAATTY 1 cut(s) 239
AfiI CCNNNNNNNGG 1 cut(s) 250
AgeI ACCGGT 1 cut(s) 202
AgsI TTSAA 1 cut(s) 23
AhdI GACNNNNNGTC 1 cut(s) 64
AluBI AGCT 2 cut(s) 97, 327
AluI AGCT 2 cut(s) 97, 327
AlwI GGATC 2 cut(s) 199, 261
AoxI GGCC 2 cut(s) 153, 180
ApeKI GCWGC 1 cut(s) 89
ApoI RAATTY 1 cut(s) 239
ArsI GACNNNNNNTTYG 2 cut(s) 231, 263
AsiGI ACCGGT 1 cut(s) 202
AspLEI GCGC 1 cut(s) 65
AspS9I GGNCC 6 cut(s) 133, 181, 200, 227, 276, 284
AsuC2I CCSGG 2 cut(s) 122, 184
AsuHPI GGTGA 1 cut(s) 66
AvaII GGWCC 5 cut(s) 133, 200, 227, 276, 284
BalI TGGCCA 1 cut(s) 155
BbvI GCAGC 1 cut(s) 76
BccI CCATC 2 cut(s) 85, 191
BceAI ACGGC 1 cut(s) 76
BcgI CGANNNNNNTGC 4 cut(s) 71, 105, 226, 260
BcnI CCSGG 2 cut(s) 122, 184
BisI GCNGC 1 cut(s) 90
BlpI GCTNAGC 1 cut(s) 93
BlsI GCNGC 1 cut(s) 91
Bme1390I CCNGG 2 cut(s) 122, 184
Bme18I GGWCC 5 cut(s) 133, 200, 227, 276, 284
BmeRI GACNNNNNGTC 1 cut(s) 64
BmgT120I GGNCC 6 cut(s) 133, 181, 200, 227, 276, 284
BmiI GGNNCC 2 cut(s) 229, 277
BmrFI CCNGG 2 cut(s) 122, 184
BmrI ACTGGG 1 cut(s) 279
BmsI GCATC 1 cut(s) 129
BmuI ACTGGG 1 cut(s) 279
Bpu10I CCTNAGC 1 cut(s) 143
Bpu1102I GCTNAGC 1 cut(s) 93
BpuMI CCSGG 2 cut(s) 122, 184
BsaJI CCNNGG 1 cut(s) 120
BsaWI WCCGGW 2 cut(s) 135, 202
Bsc4I CCNNNNNNNGG 1 cut(s) 250
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 2 cut(s) 193, 274
Bse3DI GCAATG 1 cut(s) 84
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 2 cut(s) 77, 144
BseLI CCNNNNNNNGG 1 cut(s) 250
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 1 cut(s) 107
BseNI ACTGG 2 cut(s) 193, 274
BseXI GCAGC 1 cut(s) 76
BsgI GTGCAG 2 cut(s) 72, 243
Bsh1236I CGCG 1 cut(s) 65
BshFI GGCC 2 cut(s) 155, 182
BshTI ACCGGT 1 cut(s) 202
BsiSI CCGG 4 cut(s) 122, 136, 184, 203
BslI CCNNNNNNNGG 1 cut(s) 250
BsnI GGCC 2 cut(s) 155, 182
Bsp143I GATC 2 cut(s) 191, 253
Bsp1720I GCTNAGC 1 cut(s) 93
BspANI GGCC 2 cut(s) 155, 182
BspCNI CTCAG 1 cut(s) 106
BspFNI CGCG 1 cut(s) 65
BspLI GGNNCC 2 cut(s) 229, 277
BspPI GGATC 2 cut(s) 199, 261
BsrDI GCAATG 1 cut(s) 84
BsrFI RCCGGY 1 cut(s) 202
BsrI ACTGG 2 cut(s) 193, 274
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 2 cut(s) 191, 253
BstC8I GCNNGC 2 cut(s) 55, 157
BstDEI CTNAG 3 cut(s) 93, 105, 143
BstENI CCTNNNNNAGG 1 cut(s) 248
BstF5I GGATG 2 cut(s) 77, 144
BstFNI CGCG 1 cut(s) 65
BstHHI GCGC 1 cut(s) 65
BstKTI GATC 2 cut(s) 194, 256
BstMBI GATC 2 cut(s) 191, 253
BstSCI CCNGG 2 cut(s) 120, 182
BstUI CGCG 1 cut(s) 65
BstV1I GCAGC 1 cut(s) 76
BsuRI GGCC 2 cut(s) 155, 182
BtsCI GGATG 2 cut(s) 77, 144
Cac8I GCNNGC 2 cut(s) 55, 157
CfoI GCGC 1 cut(s) 65
Cfr10I RCCGGY 1 cut(s) 202
Cfr13I GGNCC 6 cut(s) 133, 181, 200, 227, 276, 284
CseI GACGC 1 cut(s) 54
CspAI ACCGGT 1 cut(s) 202
CviAII CATG 1 cut(s) 319
CviJI RGCY 7 cut(s) 17, 97, 148, 155, 159, 182, 327
CviKI_1 RGCY 7 cut(s) 17, 97, 148, 155, 159, 182, 327
DdeI CTNAG 3 cut(s) 93, 105, 143
DpnI GATC 2 cut(s) 193, 255
DpnII GATC 2 cut(s) 191, 253
DriI GACNNNNNGTC 1 cut(s) 64
EaeI YGGCCR 1 cut(s) 153
Eam1105I GACNNNNNGTC 1 cut(s) 64
Eco47I GGWCC 5 cut(s) 133, 200, 227, 276, 284
EcoNI CCTNNNNNAGG 1 cut(s) 248
EcoO109I RGGNCCY 1 cut(s) 227
FaeI CATG 1 cut(s) 322
FaiI YATR 6 cut(s) 35, 167, 219, 233, 235, 320
FatI CATG 1 cut(s) 318
FauNDI CATATG 1 cut(s) 233
Fnu4HI GCNGC 1 cut(s) 90
FokI GGATG 2 cut(s) 64, 151
Fsp4HI GCNGC 1 cut(s) 90
GlaI GCGC 1 cut(s) 64
GluI GCNGC 1 cut(s) 90
HaeIII GGCC 2 cut(s) 155, 182
HapII CCGG 4 cut(s) 122, 136, 184, 203
HgaI GACGC 1 cut(s) 54
HhaI GCGC 1 cut(s) 65
Hin1II CATG 1 cut(s) 322
Hin6I GCGC 1 cut(s) 63
HinP1I GCGC 1 cut(s) 63
HpaII CCGG 4 cut(s) 122, 136, 184, 203
HphI GGTGA 1 cut(s) 66
Hpy188I TCNGA 2 cut(s) 82, 196
Hpy188III TCNNGA 2 cut(s) 20, 131
Hpy99I CGWCG 1 cut(s) 62
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 4 cut(s) 53, 224, 237, 301
HpyF3I CTNAG 3 cut(s) 93, 105, 143
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 1 cut(s) 322
HspAI GCGC 1 cut(s) 63
Kzo9I GATC 2 cut(s) 191, 253
LmnI GCTCC 1 cut(s) 102
Lsp1109I GCAGC 1 cut(s) 76
LweI GCATC 1 cut(s) 129
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 1 cut(s) 66
MalI GATC 2 cut(s) 193, 255
MboI GATC 2 cut(s) 191, 253
MlsI TGGCCA 1 cut(s) 155
MluCI AATT 1 cut(s) 239
MluNI TGGCCA 1 cut(s) 155
MmeI TCCRAC 2 cut(s) 105, 138
MnlI CCTC 4 cut(s) 204, 254, 257, 297
Mox20I TGGCCA 1 cut(s) 155
MscI TGGCCA 1 cut(s) 155
Msp20I TGGCCA 1 cut(s) 155
MspI CCGG 4 cut(s) 122, 136, 184, 203
MspR9I CCNGG 2 cut(s) 122, 184
MvnI CGCG 1 cut(s) 65
NciI CCSGG 2 cut(s) 122, 184
NdeI CATATG 1 cut(s) 233
NdeII GATC 2 cut(s) 191, 253
NlaIII CATG 1 cut(s) 322
NlaIV GGNNCC 2 cut(s) 229, 277
NmeAIII GCCGAG 1 cut(s) 158
NmuCI GTSAC 1 cut(s) 66
PinAI ACCGGT 1 cut(s) 202
PkrI GCNGC 1 cut(s) 91
PpuMI RGGWCCY 1 cut(s) 227
Psp5II RGGWCCY 1 cut(s) 227
PspN4I GGNNCC 2 cut(s) 229, 277
PspPI GGNCC 6 cut(s) 133, 181, 200, 227, 276, 284
PspPPI RGGWCCY 1 cut(s) 227
SatI GCNGC 1 cut(s) 90
Sau3AI GATC 2 cut(s) 191, 253
Sau96I GGNCC 6 cut(s) 133, 181, 200, 227, 276, 284
ScrFI CCNGG 2 cut(s) 122, 184
SetI ASST 5 cut(s) 33, 99, 215, 315, 329
SfaNI GCATC 1 cut(s) 129
SinI GGWCC 5 cut(s) 133, 200, 227, 276, 284
Sse9I AATT 1 cut(s) 239
StyD4I CCNGG 2 cut(s) 120, 182
TaiI ACGT 1 cut(s) 33
TasI AATT 1 cut(s) 239
TseFI GTSAC 1 cut(s) 66
TseI GCWGC 1 cut(s) 89
Tsp45I GTSAC 1 cut(s) 66
TspDTI ATGAA 1 cut(s) 307
TspGWI ACGGA 1 cut(s) 268
VpaK11BI GGWCC 5 cut(s) 133, 200, 227, 276, 284
XagI CCTNNNNNAGG 1 cut(s) 248
XapI RAATTY 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.