Rorug06G0256700
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005581
Physical Location & Seq
Reverse (-)
40358045 .. 40358761
717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0256700.1

Sequence Viewer

Length: 423 bp
ATGGAACCACAAACCCAGCAACCAACCGAGGATGGAGGAAGCAACAAAGCAGCCGGGAGTAGCGCTAACATGCTGCGGGGGCGGAACAGGTGGAATCCCACTACAGATCAGATAAGAATCCTCAAGGAGCTTTTCTACAATAAGGGAGTTAGGTCCCCAACTATAGAGCAGATTCAGAGGATCTGTCTCCAGTTGAAATGGTACGGCAATCTCGAGTTCAAGAACGTCTATTTTTGGTTCGTGAACCAAAGGGCTCGGGAGAAGTGGAAGAAGAGGTCCACTTCGGATGTTCATGTGCCCATGCAAAGTTCAGGGCTTACTACTTCCGATGGAGGCGGCGGTCACGGTGGTGGCTCTCACACTTCCCTTGAGCTCAGCCTTTCCTGCGAGGGCAAAGCTGGAAAAAGTTTCCGGAGCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

140

Amino Acids

15.72

Weight (kDa)

9.95

Isoelectric Point (pI)

61.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 28 - 88 1.1e-10 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 411
AciI CCGC 5 cut(s) 76, 82, 336, 339, 418
AclWI GGATC 1 cut(s) 188
AfaI GTAC 1 cut(s) 203
AfeI AGCGCT 1 cut(s) 64
AgsI TTSAA 2 cut(s) 196, 220
AleI CACNNNNGTG 1 cut(s) 348
AluBI AGCT 3 cut(s) 130, 373, 398
AluI AGCT 3 cut(s) 130, 373, 398
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 1 cut(s) 188
Ama87I CYCGRG 2 cut(s) 212, 255
Aor13HI TCCGGA 1 cut(s) 411
Aor51HI AGCGCT 1 cut(s) 64
ApeKI GCWGC 2 cut(s) 50, 73
AspLEI GCGC 1 cut(s) 65
AspS9I GGNCC 2 cut(s) 153, 276
AsuC2I CCSGG 1 cut(s) 55
AvaI CYCGRG 2 cut(s) 212, 255
AvaII GGWCC 2 cut(s) 153, 276
BaeGI GKGCMC 1 cut(s) 300
BanII GRGCYC 2 cut(s) 256, 375
Bbv12I GWGCWC 1 cut(s) 375
BbvI GCAGC 2 cut(s) 60, 62
BccI CCATC 2 cut(s) 26, 323
BceAI ACGGC 1 cut(s) 220
BcnI CCSGG 1 cut(s) 55
BcoDI GTCTC 1 cut(s) 191
BfmI CTRYAG 2 cut(s) 102, 162
BfoI RGCGCY 1 cut(s) 66
BisI GCNGC 4 cut(s) 51, 74, 337, 418
BlpI GCTNAGC 1 cut(s) 374
BlsI GCNGC 4 cut(s) 52, 75, 338, 419
Bme1390I CCNGG 1 cut(s) 55
Bme18I GGWCC 2 cut(s) 153, 276
BmeT110I CYCGRG 2 cut(s) 212, 255
BmgT120I GGNCC 2 cut(s) 153, 276
BmiI GGNNCC 3 cut(s) 6, 155, 416
BmrFI CCNGG 1 cut(s) 55
BpmI CTGGAG 1 cut(s) 173
Bpu1102I GCTNAGC 1 cut(s) 374
BpuEI CTTGAG 2 cut(s) 107, 389
BpuMI CCSGG 1 cut(s) 55
BsaBI GATNNNNATC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 27
BsaWI WCCGGW 1 cut(s) 411
Bse1I ACTGG 1 cut(s) 190
Bse8I GATNNNNATC 1 cut(s) 116
BseAI TCCGGA 1 cut(s) 411
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 2 cut(s) 37, 292
BseJI GATNNNNATC 1 cut(s) 116
BseMII CTCAG 1 cut(s) 388
BseNI ACTGG 1 cut(s) 190
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 2 cut(s) 60, 62
BseYI CCCAGC 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 375
BsiHKCI CYCGRG 2 cut(s) 212, 255
BsiSI CCGG 2 cut(s) 54, 412
BslFI GGGAC 1 cut(s) 139
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 139
BsoBI CYCGRG 2 cut(s) 212, 255
Bsp1286I GDGCHC 3 cut(s) 256, 300, 375
Bsp13I TCCGGA 1 cut(s) 411
Bsp143I GATC 2 cut(s) 106, 180
Bsp1720I GCTNAGC 1 cut(s) 374
BspACI CCGC 5 cut(s) 76, 82, 336, 339, 418
BspCNI CTCAG 1 cut(s) 387
BspEI TCCGGA 1 cut(s) 411
BspLI GGNNCC 3 cut(s) 6, 155, 416
BspPI GGATC 1 cut(s) 188
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 2 cut(s) 106, 180
Bst4CI ACNGT 1 cut(s) 347
Bst6I CTCTTC 1 cut(s) 266
BstDEI CTNAG 1 cut(s) 374
BstF5I GGATG 2 cut(s) 37, 292
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 1 cut(s) 65
BstKTI GATC 2 cut(s) 109, 183
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 2 cut(s) 106, 180
BstMWI GCNNNNNNNGC 2 cut(s) 79, 384
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 1 cut(s) 53
BstSFI CTRYAG 2 cut(s) 102, 162
BstSLI GKGCMC 1 cut(s) 300
BstV1I GCAGC 2 cut(s) 60, 62
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BtsCI GGATG 2 cut(s) 37, 292
CfoI GCGC 1 cut(s) 65
Cfr13I GGNCC 2 cut(s) 153, 276
Csp6I GTAC 1 cut(s) 202
CviAII CATG 3 cut(s) 70, 293, 301
CviJI RGCY 9 cut(s) 53, 130, 254, 316, 354, 373, 378, 398, 417
CviKI_1 RGCY 9 cut(s) 53, 130, 254, 316, 354, 373, 378, 398, 417
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 1 cut(s) 374
DpnI GATC 2 cut(s) 108, 182
DpnII GATC 2 cut(s) 106, 180
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
EciI GGCGGA 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 373
Eco24I GRGCYC 2 cut(s) 256, 375
Eco47I GGWCC 2 cut(s) 153, 276
Eco47III AGCGCT 1 cut(s) 64
Eco53kI GAGCTC 1 cut(s) 373
Eco88I CYCGRG 2 cut(s) 212, 255
EcoICRI GAGCTC 1 cut(s) 373
EcoO109I RGGNCCY 1 cut(s) 153
EcoT38I GRGCYC 2 cut(s) 256, 375
FaeI CATG 3 cut(s) 73, 296, 304
FaiI YATR 4 cut(s) 71, 164, 294, 302
FaqI GGGAC 1 cut(s) 139
FatI CATG 3 cut(s) 69, 292, 300
FauI CCCGC 1 cut(s) 69
Fnu4HI GCNGC 4 cut(s) 51, 74, 337, 418
FokI GGATG 2 cut(s) 44, 299
FriOI GRGCYC 2 cut(s) 256, 375
Fsp4HI GCNGC 4 cut(s) 51, 74, 337, 418
GlaI GCGC 1 cut(s) 64
GluI GCNGC 4 cut(s) 51, 74, 337, 418
GsaI CCCAGC 1 cut(s) 19
GsuI CTGGAG 1 cut(s) 173
HaeII RGCGCY 1 cut(s) 66
HapII CCGG 2 cut(s) 54, 412
HhaI GCGC 1 cut(s) 65
Hin1II CATG 3 cut(s) 73, 296, 304
Hin6I GCGC 1 cut(s) 63
HinP1I GCGC 1 cut(s) 63
HinfI GANTC 3 cut(s) 94, 117, 172
HpaII CCGG 2 cut(s) 54, 412
Hpy166II GTNNAC 2 cut(s) 244, 279
Hpy188I TCNGA 4 cut(s) 111, 177, 286, 328
Hpy188III TCNNGA 5 cut(s) 212, 220, 241, 257, 412
Hpy8I GTNNAC 2 cut(s) 244, 279
HpyCH4III ACNGT 1 cut(s) 347
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 1 cut(s) 304
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 384
HpyF3I CTNAG 1 cut(s) 374
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 3 cut(s) 73, 296, 304
HspAI GCGC 1 cut(s) 63
Kpn2I TCCGGA 1 cut(s) 411
Kzo9I GATC 2 cut(s) 106, 180
LmnI GCTCC 2 cut(s) 127, 414
LpnPI CCDG 7 cut(s) 29, 67, 73, 203, 297, 384, 397
Lsp1109I GCAGC 2 cut(s) 60, 62
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 341
MalI GATC 2 cut(s) 108, 182
MboI GATC 2 cut(s) 106, 180
MboII GAAGA 2 cut(s) 280, 283
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 3 cut(s) 256, 300, 375
MnlI CCTC 7 cut(s) 22, 29, 131, 171, 267, 326, 382
MroI TCCGGA 1 cut(s) 411
MslI CAYNNNNRTG 1 cut(s) 348
MspA1I CMGCKG 1 cut(s) 420
MspI CCGG 2 cut(s) 54, 412
MspR9I CCNGG 1 cut(s) 55
MwoI GCNNNNNNNGC 2 cut(s) 79, 384
NciI CCSGG 1 cut(s) 55
NdeII GATC 2 cut(s) 106, 180
NlaIII CATG 3 cut(s) 73, 296, 304
NlaIV GGNNCC 3 cut(s) 6, 155, 416
NmuCI GTSAC 1 cut(s) 341
NspI RCATGY 1 cut(s) 73
OliI CACNNNNGTG 1 cut(s) 348
PaeR7I CTCGAG 1 cut(s) 212
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PfeI GAWTC 3 cut(s) 94, 117, 172
PkrI GCNGC 4 cut(s) 52, 75, 338, 419
PpuMI RGGWCCY 1 cut(s) 153
Psp124BI GAGCTC 1 cut(s) 375
Psp5II RGGWCCY 1 cut(s) 153
PspFI CCCAGC 1 cut(s) 15
PspN4I GGNNCC 3 cut(s) 6, 155, 416
PspPI GGNCC 2 cut(s) 153, 276
PspPPI RGGWCCY 1 cut(s) 153
PsuI RGATCY 1 cut(s) 180
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
RseI CAYNNNNRTG 1 cut(s) 348
SacI GAGCTC 1 cut(s) 375
SatI GCNGC 4 cut(s) 51, 74, 337, 418
Sau3AI GATC 2 cut(s) 106, 180
Sau96I GGNCC 2 cut(s) 153, 276
ScrFI CCNGG 1 cut(s) 55
SduI GDGCHC 3 cut(s) 256, 300, 375
SetI ASST 7 cut(s) 92, 132, 155, 228, 278, 375, 400
SfcI CTRYAG 2 cut(s) 102, 162
Sfr274I CTCGAG 1 cut(s) 212
SinI GGWCC 2 cut(s) 153, 276
SlaI CTCGAG 1 cut(s) 212
SmiMI CAYNNNNRTG 1 cut(s) 348
SmlI CTYRAG 3 cut(s) 122, 212, 368
SmoI CTYRAG 3 cut(s) 122, 212, 368
SsiI CCGC 5 cut(s) 76, 82, 336, 339, 418
SstI GAGCTC 1 cut(s) 375
StyD4I CCNGG 1 cut(s) 53
TaaI ACNGT 1 cut(s) 347
TaiI ACGT 1 cut(s) 228
TaqI TCGA 1 cut(s) 213
TauI GCSGC 2 cut(s) 339, 420
TfiI GAWTC 3 cut(s) 94, 117, 172
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 2 cut(s) 50, 73
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 1 cut(s) 281
VpaK11BI GGWCC 2 cut(s) 153, 276
XceI RCATGY 1 cut(s) 73
XhoI CTCGAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.