Rh1AG098200

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0013781
Physical Location & Seq
Forward (+)
17142204 .. 17145448
3245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG098200.1

Sequence Viewer

Length: 636 bp
ATGATTCTTTGCCCTTGCAAAGATTGCCGTAATGTAAGCCATCAAGATAGTACTACTGTTTTTGAACATTTGGTTATAAAGGGGATGGATTCCAAGTACGAGAAAAGATCACATTGGACAAAACATGGAGATCAGATAGTAAATGCTGAAAGTGTTGATCATGAAACCACCAAGGATGAGGCATATAACTTATATAGAGCTGCCTACTTTATGGATGAAGATTCTGTCCGGCCTACAAACTCTATGGATGAAGCTACTGTACAGAGGCAAGATGATCAATTCATGAAGAACCTCGAAGATGCAGAAACTCCTTTATACCCAGGTCGTCCAATTTCTAAGGCTACATCGATTACATTGACTGACAAAGAAAAAGACATAGCACATTGTTCTGTGCTACTGAACACAGCCATAATGGATCCATTTTTAGATATGCATTTGGAGGAGTTGCAAGCAAAAGACAAACGACTTGCAAATAATGAAACCTTACTTTGGAACCGGCATACCGAGAGATTTGCAGAATGGACAAAAAACAAGATTCCTATTGACTCGAAGAATCACTCCAACACATTAAGATGGTTGGCATATGGTCCTAGAAAGAGTGCACTATCAGTTAAAGGATACATTATCAATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transpos_assoc Transposase_21

Protein Analysis

211

Amino Acids

24.38

Weight (kDa)

5.99

Isoelectric Point (pI)

31.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transpos_assoc PF13963 2 - 43 1.2e-10 Transposase-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 77
AclWI GGATC 2 cut(s) 410, 423
AfaI GTAC 3 cut(s) 52, 98, 261
AfiI CCNNNNNNNGG 1 cut(s) 489
AgsI TTSAA 1 cut(s) 65
AjnI CCWGG 1 cut(s) 319
AluBI AGCT 2 cut(s) 200, 254
AluI AGCT 2 cut(s) 200, 254
Alw21I GWGCWC 1 cut(s) 604
Alw44I GTGCAC 1 cut(s) 600
AlwI GGATC 2 cut(s) 410, 423
AoxI GGCC 1 cut(s) 230
ApaLI GTGCAC 1 cut(s) 600
ApeKI GCWGC 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 587
AvaII GGWCC 1 cut(s) 587
BaeGI GKGCMC 1 cut(s) 604
BamHI GGATCC 1 cut(s) 415
BarI GAAGNNNNNNTAC 2 cut(s) 243, 275
Bbv12I GWGCWC 1 cut(s) 604
BbvI GCAGC 1 cut(s) 187
BccI CCATC 3 cut(s) 48, 79, 567
BceAI ACGGC 1 cut(s) 12
BcgI CGANNNNNNTGC 2 cut(s) 494, 528
BciT130I CCWGG 1 cut(s) 321
BciVI GTATCC 1 cut(s) 611
BclI TGATCA 2 cut(s) 157, 274
BfaI CTAG 1 cut(s) 591
BfuI GTATCC 1 cut(s) 611
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
BmcAI AGTACT 1 cut(s) 52
Bme1390I CCNGG 1 cut(s) 321
Bme18I GGWCC 1 cut(s) 587
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 2 cut(s) 417, 494
BmrFI CCNGG 1 cut(s) 321
BmsI GCATC 1 cut(s) 289
Bsa29I ATCGAT 1 cut(s) 347
BsaJI CCNNGG 2 cut(s) 171, 319
Bsc4I CCNNNNNNNGG 1 cut(s) 489
Bse118I RCCGGY 1 cut(s) 495
BseBI CCWGG 1 cut(s) 321
BseCI ATCGAT 1 cut(s) 347
BseDI CCNNGG 2 cut(s) 171, 319
BseGI GGATG 4 cut(s) 90, 181, 220, 253
BseLI CCNNNNNNNGG 1 cut(s) 489
BseRI GAGGAG 1 cut(s) 455
BseSI GKGCMC 1 cut(s) 604
BseXI GCAGC 1 cut(s) 187
BshFI GGCC 1 cut(s) 232
BshVI ATCGAT 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 604
BsiSI CCGG 2 cut(s) 229, 496
BslI CCNNNNNNNGG 1 cut(s) 489
BsnI GGCC 1 cut(s) 232
Bsp1286I GDGCHC 1 cut(s) 604
Bsp1407I TGTACA 1 cut(s) 259
Bsp143I GATC 5 cut(s) 107, 130, 157, 274, 415
BspANI GGCC 1 cut(s) 232
BspDI ATCGAT 1 cut(s) 347
BspHI TCATGA 2 cut(s) 160, 282
BspLI GGNNCC 2 cut(s) 417, 494
BspPI GGATC 2 cut(s) 410, 423
BsrFI RCCGGY 1 cut(s) 495
BsrGI TGTACA 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 495
BssECI CCNNGG 2 cut(s) 171, 319
BssMI GATC 5 cut(s) 107, 130, 157, 274, 415
BssT1I CCWWGG 1 cut(s) 171
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 2 cut(s) 58, 259
BstAPI GCANNNNNTGC 1 cut(s) 24
BstAUI TGTACA 1 cut(s) 259
BstC8I GCNNGC 1 cut(s) 450
BstDEI CTNAG 1 cut(s) 336
BstF5I GGATG 4 cut(s) 90, 181, 220, 253
BstKTI GATC 5 cut(s) 110, 133, 160, 277, 418
BstMBI GATC 5 cut(s) 107, 130, 157, 274, 415
BstMWI GCNNNNNNNGC 1 cut(s) 24
BstNI CCWGG 1 cut(s) 321
BstSCI CCNGG 1 cut(s) 319
BstSLI GKGCMC 1 cut(s) 604
BstV1I GCAGC 1 cut(s) 187
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
Bsu15I ATCGAT 1 cut(s) 347
BsuI GTATCC 1 cut(s) 611
BsuRI GGCC 1 cut(s) 232
BsuTUI ATCGAT 1 cut(s) 347
BtsCI GGATG 4 cut(s) 90, 181, 220, 253
Cac8I GCNNGC 1 cut(s) 450
CciI TCATGA 2 cut(s) 160, 282
Cfr10I RCCGGY 1 cut(s) 495
Cfr13I GGNCC 1 cut(s) 587
ClaI ATCGAT 1 cut(s) 347
Csp6I GTAC 3 cut(s) 51, 97, 260
CviAII CATG 3 cut(s) 125, 161, 283
CviJI RGCY 6 cut(s) 39, 200, 232, 254, 341, 407
CviKI_1 RGCY 6 cut(s) 39, 200, 232, 254, 341, 407
CviQI GTAC 3 cut(s) 51, 97, 260
DdeI CTNAG 1 cut(s) 336
DpnI GATC 5 cut(s) 109, 132, 159, 276, 417
DpnII GATC 5 cut(s) 107, 130, 157, 274, 415
Eco130I CCWWGG 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 587
EcoRII CCWGG 1 cut(s) 319
EcoT14I CCWWGG 1 cut(s) 171
EcoT22I ATGCAT 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 3 cut(s) 128, 164, 286
FatI CATG 3 cut(s) 124, 160, 282
FauNDI CATATG 1 cut(s) 583
FbaI TGATCA 2 cut(s) 157, 274
Fnu4HI GCNGC 1 cut(s) 201
FokI GGATG 4 cut(s) 97, 188, 227, 260
Fsp4HI GCNGC 1 cut(s) 201
FspBI CTAG 1 cut(s) 591
GluI GCNGC 1 cut(s) 201
HaeIII GGCC 1 cut(s) 232
HapII CCGG 2 cut(s) 229, 496
Hin1II CATG 3 cut(s) 128, 164, 286
HinfI GANTC 6 cut(s) 4, 89, 221, 535, 545, 553
HpaII CCGG 2 cut(s) 229, 496
Hpy166II GTNNAC 1 cut(s) 602
Hpy188I TCNGA 1 cut(s) 135
Hpy188III TCNNGA 3 cut(s) 44, 161, 283
Hpy8I GTNNAC 1 cut(s) 602
HpyCH4III ACNGT 2 cut(s) 58, 259
HpyCH4V TGCA 7 cut(s) 18, 302, 433, 448, 470, 515, 602
HpyF10VI GCNNNNNNNGC 1 cut(s) 24
HpyF3I CTNAG 1 cut(s) 336
Hsp92II CATG 3 cut(s) 128, 164, 286
Ksp22I TGATCA 2 cut(s) 157, 274
Kzo9I GATC 5 cut(s) 107, 130, 157, 274, 415
LpnPI CCDG 4 cut(s) 242, 306, 333, 509
Lsp1109I GCAGC 1 cut(s) 187
LweI GCATC 1 cut(s) 289
MaeI CTAG 1 cut(s) 591
MalI GATC 5 cut(s) 109, 132, 159, 276, 417
MboI GATC 5 cut(s) 107, 130, 157, 274, 415
MboII GAAGA 4 cut(s) 230, 298, 308, 562
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 1 cut(s) 604
MluCI AATT 2 cut(s) 278, 330
MlyI GAGTC 1 cut(s) 539
MmeI TCCRAC 1 cut(s) 585
MnlI CCTC 4 cut(s) 172, 258, 302, 433
Mph1103I ATGCAT 1 cut(s) 435
MseI TTAA 2 cut(s) 569, 612
MslI CAYNNNNRTG 1 cut(s) 571
MspI CCGG 2 cut(s) 229, 496
MspR9I CCNGG 1 cut(s) 321
MvaI CCWGG 1 cut(s) 321
MwoI GCNNNNNNNGC 1 cut(s) 24
NdeI CATATG 1 cut(s) 583
NdeII GATC 5 cut(s) 107, 130, 157, 274, 415
NlaIII CATG 3 cut(s) 128, 164, 286
NlaIV GGNNCC 2 cut(s) 417, 494
NsiI ATGCAT 1 cut(s) 435
PagI TCATGA 2 cut(s) 160, 282
PfeI GAWTC 5 cut(s) 4, 89, 221, 535, 553
PkrI GCNGC 1 cut(s) 202
PleI GAGTC 1 cut(s) 539
PpsI GAGTC 1 cut(s) 539
PsiI TTATAA 1 cut(s) 77
Psp6I CCWGG 1 cut(s) 319
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 2 cut(s) 417, 494
PspPI GGNCC 1 cut(s) 587
PsuI RGATCY 1 cut(s) 415
RsaI GTAC 3 cut(s) 52, 98, 261
RsaNI GTAC 3 cut(s) 51, 97, 260
RseI CAYNNNNRTG 1 cut(s) 571
SaqAI TTAA 2 cut(s) 569, 612
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 5 cut(s) 107, 130, 157, 274, 415
Sau96I GGNCC 1 cut(s) 587
ScaI AGTACT 1 cut(s) 52
SchI GAGTC 1 cut(s) 539
ScrFI CCNGG 1 cut(s) 321
SduI GDGCHC 1 cut(s) 604
SetI ASST 5 cut(s) 202, 256, 294, 325, 485
SfaNI GCATC 1 cut(s) 289
SinI GGWCC 1 cut(s) 587
SmiMI CAYNNNNRTG 1 cut(s) 571
Sse9I AATT 2 cut(s) 278, 330
SspMI CTAG 1 cut(s) 591
StyD4I CCNGG 1 cut(s) 319
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 2 cut(s) 58, 259
TaqI TCGA 3 cut(s) 294, 347, 548
TasI AATT 2 cut(s) 278, 330
TatI WGTACW 2 cut(s) 50, 259
TfiI GAWTC 5 cut(s) 4, 89, 221, 535, 553
Tru1I TTAA 2 cut(s) 569, 612
Tru9I TTAA 2 cut(s) 569, 612
TseI GCWGC 1 cut(s) 200
TspDTI ATGAA 6 cut(s) 177, 231, 264, 271, 299, 492
VneI GTGCAC 1 cut(s) 600
VpaK11BI GGWCC 1 cut(s) 587
XspI CTAG 1 cut(s) 591
ZrmI AGTACT 1 cut(s) 52
Zsp2I ATGCAT 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.