Rh4BG427700

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0013781
Physical Location & Seq
Reverse (-)
57130036 .. 57136101
6066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG427700.1

Sequence Viewer

Length: 1101 bp
ATGATGGATAAAGAGTGGGTGTGGCTTCCCAGATCTAGTTCAAAGTATGAGCAAAGAGCATTACATTTTGTGAAGACGGCTGAAAAGAATTTGGGATATCCTGCAATGATTCTTTGCCCTTGCAAAGATTGCCGTAATGTAAGCCATCAAGATAGTACTACTGTTTTTGAACATTTGGTTATAAAGGGGATGGATTCCAAGTACAAGAAAAGATCACATTGGACAAAACATGGAGATCAGATAGTAAATGCTAAAAGTGTTGATCATGAAACCACCAAGGATGAGGCATATAACTTATATAGAGCTGCCTACTTTATGGATGAAGATTCTGTCCAGCCTACAAACTCTATGGATGAAGCTACTGACGAAACATTTCTCTTGCTCTTAAAGATTTTCCAAATGATTTTGGAAAAAGTGGGAACAAAAAATGGAAAAGGAATAATAATGAAGATGACACCAGTGATGTCGATGATCAGAATGAGCTTTCTAGGTCGTCCAATTTCTAAGGCTACATCGATTACATTGACTGACAAAGAGAAAGACATAGCACATCGTTCTGTGCTACTGAACACAGCCATAATGGATCCATTTTTAGATATGCATTTGGAGGAGTTGCAAGCAAAAGACAGACGACTTGCAAATAATGAAACCTTACTTTGGAACCGGCATACCGAGAGATTTGCAGAATGGACAAAAAACAAGATTCCTATTGACTCGAAGAATCACTCAAACACATTAAGATGGTTGGCATATGGTCCTAGAAAGAGTGCACTATTAGTTAAAGGATACATTATCAATGGACAGCGGTTTCACACGAAGGATGTTGATAGAGAAACTCAAAATAGTGTAGTGACTTATGATGCAATAACCATGTGTAGAGCAAGTCGCAGTAAGAGGAAGCATACTAAGAAAACCGGTGGAATATCACAAGACTTTGATGCAAGGGATGTGAATCTCATGGCAGATCTCCAAGAAGTACATACACAAGTTAGTAAAGAAGCTACCCAAGATGGACCAATTCCAAGTAATGATAGTTCTGAACAAGATGCGGTCCAAGAAAATGCATCTGTACAAGATACAGTTGAAGATCCAAGAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transpos_assoc Transposase_21

Protein Analysis

366

Amino Acids

41.97

Weight (kDa)

8.18

Isoelectric Point (pI)

35.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transpos_assoc PF13963 4 - 78 1.6e-15 Transposase-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 182
AciI CCGC 2 cut(s) 805, 1049
AclWI GGATC 3 cut(s) 578, 591, 1082
AcsI RAATTY 1 cut(s) 88
AfaI GTAC 4 cut(s) 157, 203, 978, 1071
AfiI CCNNNNNNNGG 1 cut(s) 657
AgeI ACCGGT 1 cut(s) 914
AgsI TTSAA 3 cut(s) 42, 170, 1085
AluBI AGCT 4 cut(s) 305, 359, 483, 1001
AluI AGCT 4 cut(s) 305, 359, 483, 1001
Alw21I GWGCWC 1 cut(s) 772
Alw44I GTGCAC 1 cut(s) 768
AlwI GGATC 3 cut(s) 578, 591, 1082
ApaLI GTGCAC 1 cut(s) 768
ApeKI GCWGC 1 cut(s) 305
ApoI RAATTY 1 cut(s) 88
AsiGI ACCGGT 1 cut(s) 914
Asp700I GAANNNNTTC 1 cut(s) 372
AspS9I GGNCC 3 cut(s) 755, 1013, 1051
AvaII GGWCC 3 cut(s) 755, 1013, 1051
BaeGI GKGCMC 1 cut(s) 772
BamHI GGATCC 1 cut(s) 583
BbsI GAAGAC 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 772
BbvI GCAGC 1 cut(s) 292
BccI CCATC 4 cut(s) 153, 184, 735, 1004
BceAI ACGGC 2 cut(s) 93, 117
BcgI CGANNNNNNTGC 2 cut(s) 662, 696
BciVI GTATCC 1 cut(s) 779
BclI TGATCA 2 cut(s) 262, 471
BfaI CTAG 3 cut(s) 36, 488, 759
BfuI GTATCC 1 cut(s) 779
BglII AGATCT 2 cut(s) 32, 964
BisI GCNGC 1 cut(s) 306
BlsI GCNGC 1 cut(s) 307
BmcAI AGTACT 1 cut(s) 157
Bme18I GGWCC 3 cut(s) 755, 1013, 1051
BmgT120I GGNCC 3 cut(s) 755, 1013, 1051
BmiI GGNNCC 2 cut(s) 585, 662
BmsI GCATC 4 cut(s) 850, 928, 1036, 1073
BpiI GAAGAC 1 cut(s) 80
Bsa29I ATCGAT 1 cut(s) 515
BsaBI GATNNNNATC 1 cut(s) 951
BsaJI CCNNGG 1 cut(s) 276
BsaWI WCCGGW 1 cut(s) 914
Bsc4I CCNNNNNNNGG 1 cut(s) 657
Bse118I RCCGGY 2 cut(s) 663, 914
Bse1I ACTGG 1 cut(s) 458
Bse3DI GCAATG 1 cut(s) 111
Bse8I GATNNNNATC 1 cut(s) 951
BseCI ATCGAT 1 cut(s) 515
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 6 cut(s) 195, 286, 325, 358, 826, 952
BseJI GATNNNNATC 1 cut(s) 951
BseLI CCNNNNNNNGG 1 cut(s) 657
BseMI GCAATG 1 cut(s) 111
BseNI ACTGG 1 cut(s) 458
BseRI GAGGAG 1 cut(s) 623
BseSI GKGCMC 1 cut(s) 772
BseXI GCAGC 1 cut(s) 292
BshTI ACCGGT 1 cut(s) 914
BshVI ATCGAT 1 cut(s) 515
BsiHKAI GWGCWC 1 cut(s) 772
BsiSI CCGG 2 cut(s) 664, 915
BslI CCNNNNNNNGG 1 cut(s) 657
Bsp1286I GDGCHC 1 cut(s) 772
Bsp1407I TGTACA 1 cut(s) 1069
Bsp143I GATC 8 cut(s) 32, 212, 235, 262, 471, 583, 964, 1087
BspACI CCGC 2 cut(s) 805, 1049
BspDI ATCGAT 1 cut(s) 515
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 2 cut(s) 585, 662
BspPI GGATC 3 cut(s) 578, 591, 1082
BsrDI GCAATG 1 cut(s) 111
BsrFI RCCGGY 2 cut(s) 663, 914
BsrGI TGTACA 1 cut(s) 1069
BsrI ACTGG 1 cut(s) 458
BssAI RCCGGY 2 cut(s) 663, 914
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 8 cut(s) 32, 212, 235, 262, 471, 583, 964, 1087
BssT1I CCWWGG 1 cut(s) 276
Bst4CI ACNGT 2 cut(s) 163, 1081
BstAPI GCANNNNNTGC 1 cut(s) 129
BstAUI TGTACA 1 cut(s) 1069
BstC8I GCNNGC 1 cut(s) 618
BstDEI CTNAG 2 cut(s) 504, 906
BstF5I GGATG 6 cut(s) 195, 286, 325, 358, 826, 952
BstKTI GATC 8 cut(s) 35, 215, 238, 265, 474, 586, 967, 1090
BstMBI GATC 8 cut(s) 32, 212, 235, 262, 471, 583, 964, 1087
BstMWI GCNNNNNNNGC 1 cut(s) 129
BstSLI GKGCMC 1 cut(s) 772
BstV1I GCAGC 1 cut(s) 292
BstV2I GAAGAC 1 cut(s) 80
BstX2I RGATCY 4 cut(s) 32, 583, 964, 1087
BstYI RGATCY 4 cut(s) 32, 583, 964, 1087
Bsu15I ATCGAT 1 cut(s) 515
BsuI GTATCC 1 cut(s) 779
BsuTUI ATCGAT 1 cut(s) 515
BtsCI GGATG 6 cut(s) 195, 286, 325, 358, 826, 952
BtsIMutI CAGTG 1 cut(s) 465
Cac8I GCNNGC 1 cut(s) 618
CciI TCATGA 1 cut(s) 265
Cfr10I RCCGGY 2 cut(s) 663, 914
Cfr13I GGNCC 3 cut(s) 755, 1013, 1051
ClaI ATCGAT 1 cut(s) 515
Csp6I GTAC 4 cut(s) 156, 202, 977, 1070
CspAI ACCGGT 1 cut(s) 914
CviAII CATG 4 cut(s) 230, 266, 871, 958
CviQI GTAC 4 cut(s) 156, 202, 977, 1070
DdeI CTNAG 2 cut(s) 504, 906
DpnI GATC 8 cut(s) 34, 214, 237, 264, 473, 585, 966, 1089
DpnII GATC 8 cut(s) 32, 212, 235, 262, 471, 583, 964, 1087
Eco130I CCWWGG 1 cut(s) 276
Eco32I GATATC 1 cut(s) 98
Eco47I GGWCC 3 cut(s) 755, 1013, 1051
EcoRV GATATC 1 cut(s) 98
EcoT14I CCWWGG 1 cut(s) 276
EcoT22I ATGCAT 2 cut(s) 603, 1066
ErhI CCWWGG 1 cut(s) 276
FaeI CATG 4 cut(s) 233, 269, 874, 961
FatI CATG 4 cut(s) 229, 265, 870, 957
FauNDI CATATG 1 cut(s) 751
FbaI TGATCA 2 cut(s) 262, 471
Fnu4HI GCNGC 1 cut(s) 306
FokI GGATG 6 cut(s) 202, 293, 332, 365, 833, 959
Fsp4HI GCNGC 1 cut(s) 306
FspBI CTAG 3 cut(s) 36, 488, 759
GluI GCNGC 1 cut(s) 306
HapII CCGG 2 cut(s) 664, 915
Hin1II CATG 4 cut(s) 233, 269, 874, 961
HinfI GANTC 7 cut(s) 109, 194, 326, 703, 713, 721, 952
HpaII CCGG 2 cut(s) 664, 915
Hpy166II GTNNAC 1 cut(s) 770
Hpy188I TCNGA 3 cut(s) 240, 476, 1039
Hpy188III TCNNGA 2 cut(s) 149, 266
Hpy8I GTNNAC 1 cut(s) 770
HpyAV CCTTC 1 cut(s) 811
HpyCH4III ACNGT 2 cut(s) 163, 1081
HpyF10VI GCNNNNNNNGC 1 cut(s) 129
HpyF3I CTNAG 2 cut(s) 504, 906
Hsp92II CATG 4 cut(s) 233, 269, 874, 961
Ksp22I TGATCA 2 cut(s) 262, 471
Kzo9I GATC 8 cut(s) 32, 212, 235, 262, 471, 583, 964, 1087
LpnPI CCDG 6 cut(s) 43, 114, 347, 471, 677, 928
Lsp1109I GCAGC 1 cut(s) 292
LweI GCATC 4 cut(s) 850, 928, 1036, 1073
MaeI CTAG 3 cut(s) 36, 488, 759
MaeIII GTNAC 1 cut(s) 850
MalI GATC 8 cut(s) 34, 214, 237, 264, 473, 585, 966, 1089
MboI GATC 8 cut(s) 32, 212, 235, 262, 471, 583, 964, 1087
MboII GAAGA 5 cut(s) 85, 335, 460, 730, 1097
MflI RGATCY 4 cut(s) 32, 583, 964, 1087
MhlI GDGCHC 1 cut(s) 772
MluCI AATT 3 cut(s) 88, 498, 1017
MlyI GAGTC 1 cut(s) 707
MnlI CCTC 4 cut(s) 277, 601, 888, 1088
Mph1103I ATGCAT 2 cut(s) 603, 1066
MroXI GAANNNNTTC 1 cut(s) 372
MseI TTAA 3 cut(s) 386, 737, 780
MslI CAYNNNNRTG 1 cut(s) 739
MspA1I CMGCKG 1 cut(s) 805
MspI CCGG 2 cut(s) 664, 915
MwoI GCNNNNNNNGC 1 cut(s) 129
NdeI CATATG 1 cut(s) 751
NdeII GATC 8 cut(s) 32, 212, 235, 262, 471, 583, 964, 1087
NlaIII CATG 4 cut(s) 233, 269, 874, 961
NlaIV GGNNCC 2 cut(s) 585, 662
NmuCI GTSAC 1 cut(s) 850
NsiI ATGCAT 2 cut(s) 603, 1066
PagI TCATGA 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 372
PfeI GAWTC 6 cut(s) 109, 194, 326, 703, 721, 952
PinAI ACCGGT 1 cut(s) 914
PkrI GCNGC 1 cut(s) 307
PleI GAGTC 1 cut(s) 707
PpsI GAGTC 1 cut(s) 707
PsiI TTATAA 1 cut(s) 182
PspN4I GGNNCC 2 cut(s) 585, 662
PspPI GGNCC 3 cut(s) 755, 1013, 1051
PsrI GAACNNNNNNTAC 2 cut(s) 1018, 1050
PsuI RGATCY 4 cut(s) 32, 583, 964, 1087
RsaI GTAC 4 cut(s) 157, 203, 978, 1071
RsaNI GTAC 4 cut(s) 156, 202, 977, 1070
RseI CAYNNNNRTG 1 cut(s) 739
SaqAI TTAA 3 cut(s) 386, 737, 780
SatI GCNGC 1 cut(s) 306
Sau3AI GATC 8 cut(s) 32, 212, 235, 262, 471, 583, 964, 1087
Sau96I GGNCC 3 cut(s) 755, 1013, 1051
ScaI AGTACT 1 cut(s) 157
SchI GAGTC 1 cut(s) 707
SduI GDGCHC 1 cut(s) 772
SetI ASST 7 cut(s) 307, 361, 485, 493, 653, 1003, 1099
SfaNI GCATC 4 cut(s) 850, 928, 1036, 1073
SinI GGWCC 3 cut(s) 755, 1013, 1051
SmiMI CAYNNNNRTG 1 cut(s) 739
Sse9I AATT 3 cut(s) 88, 498, 1017
SsiI CCGC 2 cut(s) 805, 1049
SspMI CTAG 3 cut(s) 36, 488, 759
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 2 cut(s) 163, 1081
TaqI TCGA 3 cut(s) 467, 515, 716
TasI AATT 3 cut(s) 88, 498, 1017
TatI WGTACW 4 cut(s) 155, 201, 976, 1069
TfiI GAWTC 6 cut(s) 109, 194, 326, 703, 721, 952
Tru1I TTAA 3 cut(s) 386, 737, 780
Tru9I TTAA 3 cut(s) 386, 737, 780
TscAI CASTG 1 cut(s) 465
TseFI GTSAC 1 cut(s) 850
TseI GCWGC 1 cut(s) 305
Tsp45I GTSAC 1 cut(s) 850
TspDTI ATGAA 5 cut(s) 282, 336, 369, 461, 660
TspRI CASTG 1 cut(s) 465
VneI GTGCAC 1 cut(s) 768
VpaK11BI GGWCC 3 cut(s) 755, 1013, 1051
XapI RAATTY 1 cut(s) 88
XmnI GAANNNNTTC 1 cut(s) 372
XspI CTAG 3 cut(s) 36, 488, 759
ZrmI AGTACT 1 cut(s) 157
Zsp2I ATGCAT 2 cut(s) 603, 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.