Rh4DG423500

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0013781
Physical Location & Seq
Reverse (-)
63056368 .. 63065061
8694 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG423500.1

Sequence Viewer

Length: 1167 bp
ATGGAGGAAGAGTTTTCAGGACCTCCAATCACTTATTTGGTTGTTGAGAATTATGATGAAAGGAAGAGTGAAAAAGAAAGTGAAATTTTTGGGGTGGTGTTGTTTTGGGTGCCGAAAATTGATGTTAGTTCCATATCAGTTGAGCACATGAGAATAGTGAAATTACAAATTGTTCTGTTGTCGTTCTGTTTTGATATACATTGCTTGCATAAGTACTGCTTGAATCTAAAGATGATGGATAAAGAGTGGGTGTGGCTTCCCAGATCTAGTTCAAAGTATGAGCAAAGAGCATTACATTTTGTGAAGACGGCTGAAAAGAATTTGGGATATCCTGCAATGATTCTTTGCCCTTGCAAAGATTGCCGTAATGTAAGCCATCAAGATAGTACTACTGTTTTTGAACATTTGGTTATAAAGGGGATGGATTCCAAGTACAAGAAAAGATCACATTGGACAAAACATGGAGATCAGATAGTAAACGCTAAAAGTGTTGATCATGAAACCACCAAGGATGAGGCATATAACTTATATAGAGCTGCCTACTTTATGGATGAAGATTTTGTCCAGCCTACAAACTCTATGGATGAAGCTACTGACGAAACATTTCTCTTGCTCTTAAAGATTTTCCAAATGATTTTGGAAAAGGTGGGAACAAAAAATGGAAAAGGAATAATAATGAAGATGACACCAGTGATGTCGATGATCAGAATGAGCTTTCTAGGTCGTCCAATTTCTAAGGCTACATCGATTACATTGACTGACAAAAAGAAAGACATAGCACATCGTTCTGTGCTACTGAACACAGCCATAATGGATCCATTTTTAGATATGCATTTGGAGGAGTTGCAAGCAAAAGACAGACGACTTGCAAATAATGAAACCTTACTTTGGAACCGGCATACCGAGAGATTTGCAGAATGGACAAAAAACAAGATTCCTATTGACTCGAAGAATCACTCAAACACATTAAGATGGTTGGCATATGGTCCTAGAAAGAGTGCACTATTAGTTAAAGGATACATTATCAATGGACAGCGGTTTCACACGAAGGATGTTGATAGAGAAACTCAAAATAGTGGAGTGACTTATGATGCAATAACCATGTGTAGAGGAAGTACAAAAGATATTGCACAATGGCTGATGTGGTTACTTACTACGGGATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transpos_assoc Transposase_21

Protein Analysis

388

Amino Acids

45.17

Weight (kDa)

8.63

Isoelectric Point (pI)

32.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transpos_assoc PF13963 81 - 155 1.7e-15 Transposase-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 413
AccB1I GGYRCC 1 cut(s) 109
AciI CCGC 1 cut(s) 1036
AclWI GGATC 2 cut(s) 809, 822
AcsI RAATTY 2 cut(s) 84, 319
AfaI GTAC 4 cut(s) 215, 388, 434, 1117
AfiI CCNNNNNNNGG 1 cut(s) 888
AgsI TTSAA 3 cut(s) 223, 273, 401
AluBI AGCT 3 cut(s) 536, 590, 714
AluI AGCT 3 cut(s) 536, 590, 714
Alw21I GWGCWC 2 cut(s) 147, 1003
Alw44I GTGCAC 1 cut(s) 999
AlwI GGATC 2 cut(s) 809, 822
ApaLI GTGCAC 1 cut(s) 999
ApeKI GCWGC 1 cut(s) 536
ApoI RAATTY 2 cut(s) 84, 319
Asp700I GAANNNNTTC 1 cut(s) 603
AspS9I GGNCC 2 cut(s) 20, 986
AvaII GGWCC 2 cut(s) 20, 986
BaeGI GKGCMC 1 cut(s) 1003
BamHI GGATCC 1 cut(s) 814
BanI GGYRCC 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 311
Bbv12I GWGCWC 2 cut(s) 147, 1003
BbvI GCAGC 1 cut(s) 523
BccI CCATC 4 cut(s) 229, 384, 415, 966
BceAI ACGGC 2 cut(s) 324, 348
BcgI CGANNNNNNTGC 2 cut(s) 893, 927
BciVI GTATCC 1 cut(s) 1010
BclI TGATCA 2 cut(s) 493, 702
BfaI CTAG 3 cut(s) 267, 719, 990
BfuI GTATCC 1 cut(s) 1010
BglII AGATCT 1 cut(s) 263
BisI GCNGC 1 cut(s) 537
BlsI GCNGC 1 cut(s) 538
BmcAI AGTACT 2 cut(s) 215, 388
Bme18I GGWCC 2 cut(s) 20, 986
BmgT120I GGNCC 2 cut(s) 20, 986
BmiI GGNNCC 3 cut(s) 111, 816, 893
BmsI GCATC 1 cut(s) 1081
BpiI GAAGAC 1 cut(s) 311
Bsa29I ATCGAT 1 cut(s) 746
BsaJI CCNNGG 1 cut(s) 507
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 888
Bse118I RCCGGY 1 cut(s) 894
Bse1I ACTGG 1 cut(s) 689
Bse3DI GCAATG 2 cut(s) 199, 342
BseCI ATCGAT 1 cut(s) 746
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 5 cut(s) 426, 517, 556, 589, 1057
BseLI CCNNNNNNNGG 1 cut(s) 888
BseMI GCAATG 2 cut(s) 199, 342
BseNI ACTGG 1 cut(s) 689
BseRI GAGGAG 1 cut(s) 854
BseSI GKGCMC 1 cut(s) 1003
BseXI GCAGC 1 cut(s) 523
BshNI GGYRCC 1 cut(s) 109
BshVI ATCGAT 1 cut(s) 746
BsiHKAI GWGCWC 2 cut(s) 147, 1003
BsiSI CCGG 1 cut(s) 895
BslI CCNNNNNNNGG 1 cut(s) 888
Bsp1286I GDGCHC 2 cut(s) 147, 1003
Bsp143I GATC 6 cut(s) 263, 443, 466, 493, 702, 814
BspACI CCGC 1 cut(s) 1036
BspDI ATCGAT 1 cut(s) 746
BspHI TCATGA 1 cut(s) 496
BspLI GGNNCC 3 cut(s) 111, 816, 893
BspPI GGATC 2 cut(s) 809, 822
BspT107I GGYRCC 1 cut(s) 109
BsrDI GCAATG 2 cut(s) 199, 342
BsrFI RCCGGY 1 cut(s) 894
BsrI ACTGG 1 cut(s) 689
BssAI RCCGGY 1 cut(s) 894
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 6 cut(s) 263, 443, 466, 493, 702, 814
BssT1I CCWWGG 1 cut(s) 507
Bst4CI ACNGT 1 cut(s) 394
Bst6I CTCTTC 2 cut(s) 3, 59
BstAPI GCANNNNNTGC 1 cut(s) 360
BstC8I GCNNGC 2 cut(s) 206, 849
BstDEI CTNAG 1 cut(s) 735
BstF5I GGATG 5 cut(s) 426, 517, 556, 589, 1057
BstKTI GATC 6 cut(s) 266, 446, 469, 496, 705, 817
BstMBI GATC 6 cut(s) 263, 443, 466, 493, 702, 814
BstMWI GCNNNNNNNGC 1 cut(s) 360
BstSLI GKGCMC 1 cut(s) 1003
BstV1I GCAGC 1 cut(s) 523
BstV2I GAAGAC 1 cut(s) 311
BstX2I RGATCY 2 cut(s) 263, 814
BstYI RGATCY 2 cut(s) 263, 814
Bsu15I ATCGAT 1 cut(s) 746
BsuI GTATCC 1 cut(s) 1010
BsuTUI ATCGAT 1 cut(s) 746
BtsCI GGATG 5 cut(s) 426, 517, 556, 589, 1057
BtsIMutI CAGTG 1 cut(s) 696
Cac8I GCNNGC 2 cut(s) 206, 849
CciI TCATGA 1 cut(s) 496
Cfr10I RCCGGY 1 cut(s) 894
Cfr13I GGNCC 2 cut(s) 20, 986
ClaI ATCGAT 1 cut(s) 746
Csp6I GTAC 4 cut(s) 214, 387, 433, 1116
CviAII CATG 4 cut(s) 148, 461, 497, 1102
CviQI GTAC 4 cut(s) 214, 387, 433, 1116
DdeI CTNAG 1 cut(s) 735
DpnI GATC 6 cut(s) 265, 445, 468, 495, 704, 816
DpnII GATC 6 cut(s) 263, 443, 466, 493, 702, 814
Eam1104I CTCTTC 2 cut(s) 3, 59
EarI CTCTTC 2 cut(s) 3, 59
Eco130I CCWWGG 1 cut(s) 507
Eco32I GATATC 1 cut(s) 329
Eco47I GGWCC 2 cut(s) 20, 986
EcoO109I RGGNCCY 1 cut(s) 20
EcoRV GATATC 1 cut(s) 329
EcoT14I CCWWGG 1 cut(s) 507
EcoT22I ATGCAT 1 cut(s) 834
ErhI CCWWGG 1 cut(s) 507
FaeI CATG 4 cut(s) 151, 464, 500, 1105
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FatI CATG 4 cut(s) 147, 460, 496, 1101
FauNDI CATATG 1 cut(s) 982
FbaI TGATCA 2 cut(s) 493, 702
Fnu4HI GCNGC 1 cut(s) 537
FokI GGATG 5 cut(s) 433, 524, 563, 596, 1064
Fsp4HI GCNGC 1 cut(s) 537
FspBI CTAG 3 cut(s) 267, 719, 990
GluI GCNGC 1 cut(s) 537
HapII CCGG 1 cut(s) 895
Hin1II CATG 4 cut(s) 151, 464, 500, 1105
HinfI GANTC 6 cut(s) 223, 340, 425, 934, 944, 952
HpaII CCGG 1 cut(s) 895
Hpy166II GTNNAC 2 cut(s) 478, 1001
Hpy188I TCNGA 2 cut(s) 471, 707
Hpy188III TCNNGA 3 cut(s) 18, 380, 497
Hpy8I GTNNAC 2 cut(s) 478, 1001
HpyAV CCTTC 1 cut(s) 1042
HpyCH4III ACNGT 1 cut(s) 394
HpyF10VI GCNNNNNNNGC 1 cut(s) 360
HpyF3I CTNAG 1 cut(s) 735
Hsp92II CATG 4 cut(s) 151, 464, 500, 1105
Ksp22I TGATCA 2 cut(s) 493, 702
Kzo9I GATC 6 cut(s) 263, 443, 466, 493, 702, 814
LpnPI CCDG 6 cut(s) 3, 274, 345, 578, 702, 908
Lsp1109I GCAGC 1 cut(s) 523
LweI GCATC 1 cut(s) 1081
MaeI CTAG 3 cut(s) 267, 719, 990
MaeIII GTNAC 2 cut(s) 1081, 1146
MalI GATC 6 cut(s) 265, 445, 468, 495, 704, 816
MboI GATC 6 cut(s) 263, 443, 466, 493, 702, 814
MboII GAAGA 6 cut(s) 20, 76, 316, 566, 691, 961
MflI RGATCY 2 cut(s) 263, 814
MhlI GDGCHC 2 cut(s) 147, 1003
MluCI AATT 7 cut(s) 49, 84, 117, 161, 168, 319, 729
MlyI GAGTC 1 cut(s) 938
MnlI CCTC 4 cut(s) 33, 508, 832, 1103
Mph1103I ATGCAT 1 cut(s) 834
MroXI GAANNNNTTC 1 cut(s) 603
MseI TTAA 3 cut(s) 617, 968, 1011
MslI CAYNNNNRTG 1 cut(s) 970
MspA1I CMGCKG 1 cut(s) 1036
MspI CCGG 1 cut(s) 895
MwoI GCNNNNNNNGC 1 cut(s) 360
NdeI CATATG 1 cut(s) 982
NdeII GATC 6 cut(s) 263, 443, 466, 493, 702, 814
NlaIII CATG 4 cut(s) 151, 464, 500, 1105
NlaIV GGNNCC 3 cut(s) 111, 816, 893
NmuCI GTSAC 1 cut(s) 1081
NsiI ATGCAT 1 cut(s) 834
PagI TCATGA 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 603
PfeI GAWTC 5 cut(s) 223, 340, 425, 934, 952
PkrI GCNGC 1 cut(s) 538
PleI GAGTC 1 cut(s) 938
PpsI GAGTC 1 cut(s) 938
PpuMI RGGWCCY 1 cut(s) 20
PsiI TTATAA 1 cut(s) 413
Psp5II RGGWCCY 1 cut(s) 20
PspN4I GGNNCC 3 cut(s) 111, 816, 893
PspPI GGNCC 2 cut(s) 20, 986
PspPPI RGGWCCY 1 cut(s) 20
PsuI RGATCY 2 cut(s) 263, 814
RsaI GTAC 4 cut(s) 215, 388, 434, 1117
RsaNI GTAC 4 cut(s) 214, 387, 433, 1116
RseI CAYNNNNRTG 1 cut(s) 970
SaqAI TTAA 3 cut(s) 617, 968, 1011
SatI GCNGC 1 cut(s) 537
Sau3AI GATC 6 cut(s) 263, 443, 466, 493, 702, 814
Sau96I GGNCC 2 cut(s) 20, 986
ScaI AGTACT 2 cut(s) 215, 388
SchI GAGTC 1 cut(s) 938
SduI GDGCHC 2 cut(s) 147, 1003
SetI ASST 7 cut(s) 25, 538, 592, 648, 716, 724, 884
SfaNI GCATC 1 cut(s) 1081
SinI GGWCC 2 cut(s) 20, 986
SmiMI CAYNNNNRTG 1 cut(s) 970
Sse9I AATT 7 cut(s) 49, 84, 117, 161, 168, 319, 729
SsiI CCGC 1 cut(s) 1036
SspMI CTAG 3 cut(s) 267, 719, 990
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 1 cut(s) 394
TaqI TCGA 3 cut(s) 698, 746, 947
TasI AATT 7 cut(s) 49, 84, 117, 161, 168, 319, 729
TatI WGTACW 4 cut(s) 213, 386, 432, 1115
TfiI GAWTC 5 cut(s) 223, 340, 425, 934, 952
Tru1I TTAA 3 cut(s) 617, 968, 1011
Tru9I TTAA 3 cut(s) 617, 968, 1011
TscAI CASTG 1 cut(s) 696
TseFI GTSAC 1 cut(s) 1081
TseI GCWGC 1 cut(s) 536
Tsp45I GTSAC 1 cut(s) 1081
TspDTI ATGAA 6 cut(s) 72, 513, 567, 600, 692, 891
TspRI CASTG 1 cut(s) 696
VneI GTGCAC 1 cut(s) 999
VpaK11BI GGWCC 2 cut(s) 20, 986
XapI RAATTY 2 cut(s) 84, 319
XmnI GAANNNNTTC 1 cut(s) 603
XspI CTAG 3 cut(s) 267, 719, 990
ZrmI AGTACT 2 cut(s) 215, 388
Zsp2I ATGCAT 1 cut(s) 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.