Rh5AG490100

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0013781
Physical Location & Seq
Forward (+)
83544021 .. 83544730
710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG490100.1

Sequence Viewer

Length: 525 bp
ATGAAAACACTAAAAGATTATGTACGAAATCCTGCAAGACCTAAGGGGTGTATTGCAGAGTCCTACCTAGCAGAAGAGTGCATCACTTTTTGTAGTCAGTTTCTGAAAAAATCCATACAAGTGGAGGAAAAAGAAGTTCGGAATGAAGATTTTGTCAATGATGTCATTCTTGAAGGTCGTCCAATTTCTAAGGCTACATCGATTTCATTGACTGACAAAGAAAAAGACATAGCACATCGTTCTATGCTACTGAACACAGCCATAATGGATCCATTTTTAGATATGCATTTGGAGGAGTTGCAAGCAAAAGACAAACGACTTGCAAATAATGAAACCTTACTTTGGAACCGGCATACCGAGAGATTTGCAGAATGGACAAAAAACAAGATTCCTATTGACTCGAAGAATCACTCAAATACATTAAGATGGTTGGCATATGGTCCTAGAAAGAGTGCATTATCAGTTAAAGGATACATTATCAATGGACAGCGGTTTCACATGAAGGATGTTGATAGAGAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transpos_assoc Transposase_21

Protein Analysis

174

Amino Acids

20.27

Weight (kDa)

8.71

Isoelectric Point (pI)

38.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4218 PF13960 2 - 48 2.8e-12 Domain of unknown function (DUF4218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 490
AclWI GGATC 2 cut(s) 263, 276
AfaI GTAC 1 cut(s) 24
AfiI CCNNNNNNNGG 1 cut(s) 342
AgsI TTSAA 1 cut(s) 173
AlwI GGATC 2 cut(s) 263, 276
AlwNI CAGNNNCTG 1 cut(s) 103
AspS9I GGNCC 1 cut(s) 440
AvaII GGWCC 1 cut(s) 440
AxyI CCTNAGG 1 cut(s) 42
BamHI GGATCC 1 cut(s) 268
BccI CCATC 1 cut(s) 420
BcgI CGANNNNNNTGC 2 cut(s) 347, 381
BciVI GTATCC 1 cut(s) 464
BfaI CTAG 2 cut(s) 68, 444
BfuI GTATCC 1 cut(s) 464
Bme18I GGWCC 1 cut(s) 440
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 2 cut(s) 270, 347
BmsI GCATC 1 cut(s) 90
Bsa29I ATCGAT 1 cut(s) 200
Bsc4I CCNNNNNNNGG 1 cut(s) 342
Bse118I RCCGGY 1 cut(s) 348
Bse21I CCTNAGG 1 cut(s) 42
BseCI ATCGAT 1 cut(s) 200
BseGI GGATG 1 cut(s) 511
BseLI CCNNNNNNNGG 1 cut(s) 342
BseRI GAGGAG 1 cut(s) 308
BshVI ATCGAT 1 cut(s) 200
BsiSI CCGG 1 cut(s) 349
BslI CCNNNNNNNGG 1 cut(s) 342
Bsp143I GATC 1 cut(s) 268
BspACI CCGC 1 cut(s) 490
BspDI ATCGAT 1 cut(s) 200
BspLI GGNNCC 2 cut(s) 270, 347
BspPI GGATC 2 cut(s) 263, 276
BsrFI RCCGGY 1 cut(s) 348
BssAI RCCGGY 1 cut(s) 348
BssMI GATC 1 cut(s) 268
Bst6I CTCTTC 1 cut(s) 69
BstC8I GCNNGC 1 cut(s) 303
BstDEI CTNAG 2 cut(s) 42, 189
BstF5I GGATG 1 cut(s) 511
BstKTI GATC 1 cut(s) 271
BstMBI GATC 1 cut(s) 268
BstX2I RGATCY 1 cut(s) 268
BstXI CCANNNNNNTGG 1 cut(s) 121
BstYI RGATCY 1 cut(s) 268
Bsu15I ATCGAT 1 cut(s) 200
Bsu36I CCTNAGG 1 cut(s) 42
BsuI GTATCC 1 cut(s) 464
BsuTUI ATCGAT 1 cut(s) 200
BtsCI GGATG 1 cut(s) 511
Cac8I GCNNGC 1 cut(s) 303
CaiI CAGNNNCTG 1 cut(s) 103
Cfr10I RCCGGY 1 cut(s) 348
Cfr13I GGNCC 1 cut(s) 440
ClaI ATCGAT 1 cut(s) 200
Csp6I GTAC 1 cut(s) 23
CviAII CATG 1 cut(s) 499
CviJI RGCY 2 cut(s) 194, 260
CviKI_1 RGCY 2 cut(s) 194, 260
CviQI GTAC 1 cut(s) 23
DdeI CTNAG 2 cut(s) 42, 189
DpnI GATC 1 cut(s) 270
DpnII GATC 1 cut(s) 268
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 440
Eco81I CCTNAGG 1 cut(s) 42
EcoT22I ATGCAT 1 cut(s) 288
FaeI CATG 1 cut(s) 502
FatI CATG 1 cut(s) 498
FauNDI CATATG 1 cut(s) 436
FokI GGATG 1 cut(s) 518
FspBI CTAG 2 cut(s) 68, 444
HapII CCGG 1 cut(s) 349
Hin1II CATG 1 cut(s) 502
HinfI GANTC 4 cut(s) 59, 388, 398, 406
HpaII CCGG 1 cut(s) 349
Hpy188I TCNGA 2 cut(s) 105, 141
Hpy188III TCNNGA 1 cut(s) 170
HpyAV CCTTC 2 cut(s) 167, 496
HpyCH4V TGCA 8 cut(s) 35, 56, 81, 286, 301, 323, 368, 455
HpyF3I CTNAG 2 cut(s) 42, 189
Hsp92II CATG 1 cut(s) 502
Kzo9I GATC 1 cut(s) 268
LpnPI CCDG 2 cut(s) 45, 362
LweI GCATC 1 cut(s) 90
MaeI CTAG 2 cut(s) 68, 444
MalI GATC 1 cut(s) 270
MboI GATC 1 cut(s) 268
MboII GAAGA 3 cut(s) 86, 158, 415
MflI RGATCY 1 cut(s) 268
MluCI AATT 1 cut(s) 183
MlyI GAGTC 2 cut(s) 68, 392
MnlI CCTC 2 cut(s) 118, 286
Mph1103I ATGCAT 1 cut(s) 288
MseI TTAA 3 cut(s) 422, 465, 523
MslI CAYNNNNRTG 2 cut(s) 119, 424
MspA1I CMGCKG 1 cut(s) 490
MspI CCGG 1 cut(s) 349
NdeI CATATG 1 cut(s) 436
NdeII GATC 1 cut(s) 268
NlaIII CATG 1 cut(s) 502
NlaIV GGNNCC 2 cut(s) 270, 347
NsiI ATGCAT 1 cut(s) 288
PfeI GAWTC 2 cut(s) 388, 406
PleI GAGTC 2 cut(s) 67, 392
PpsI GAGTC 2 cut(s) 67, 392
PspN4I GGNNCC 2 cut(s) 270, 347
PspPI GGNCC 1 cut(s) 440
PstNI CAGNNNCTG 1 cut(s) 103
PsuI RGATCY 1 cut(s) 268
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
RseI CAYNNNNRTG 2 cut(s) 119, 424
SaqAI TTAA 3 cut(s) 422, 465, 523
Sau3AI GATC 1 cut(s) 268
Sau96I GGNCC 1 cut(s) 440
SchI GAGTC 2 cut(s) 68, 392
SetI ASST 4 cut(s) 43, 69, 178, 338
SfaNI GCATC 1 cut(s) 90
SinI GGWCC 1 cut(s) 440
SmiMI CAYNNNNRTG 2 cut(s) 119, 424
Sse9I AATT 1 cut(s) 183
SsiI CCGC 1 cut(s) 490
SspMI CTAG 2 cut(s) 68, 444
TaqI TCGA 2 cut(s) 200, 401
TasI AATT 1 cut(s) 183
TfiI GAWTC 2 cut(s) 388, 406
Tru1I TTAA 3 cut(s) 422, 465, 523
Tru9I TTAA 3 cut(s) 422, 465, 523
TspDTI ATGAA 5 cut(s) 17, 159, 195, 345, 515
VpaK11BI GGWCC 1 cut(s) 440
XspI CTAG 2 cut(s) 68, 444
Zsp2I ATGCAT 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.