Rh7CG255800

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0013781
Physical Location & Seq
Forward (+)
24771810 .. 24779517
7708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG255800.1

Sequence Viewer

Length: 1212 bp
ATGAATGCAGCAGAGCTGCTTGTTGATGCTTCTCAATCATCCGAACGACCAGTATCTCCAACTTTCTTTGGAATTTTCTTGGAGGAGATTAACCATGCTAGAGCTGGTGGGTTGTGGGCTGAGCTTGTAAGCAACAGAGGATATTCAAGGACAATAGGATCGTGTGGCATAGCTGGTTGGGTGCGTCTTCACAGAGCTGATCCCGAGTACGAGAAAGGGGCATGGAATTTTGTGAAAACGGTCATAAGGAACCTGGGAAACCCTGAAATGATCTTATGTCCTTGCATAGATTGTCGCAATGTAGATCATCAGCATGGCAATATTGTTGTTGAACATTTGGTTACAAGGGGAATGGAGGCCAAGTACAAACAAAGAAAAGATTGGAAGCCAAGAATCTTGACAGGTCGGAATATTTCAGTTGCTCTTAGAAATTTTCCAAATGATTTTGGAAAAGGTAGTAACAAAAAAAGAAAAAGGAATGACAATGCTGATCCATATTCTGATGGTGAAGACAATGATGATGTTAATGATGACTATGATGATCAGGAGGAGCTATCAAGTGAGTTTCTGAAAAAAACAATACACGTGGAGGAAAAACAAGTTCGCAATGATGATTTTGGCAATGAAGTGATTCTCAAAGGTCGTCCAATTTCTGGAGCCACATCGATCACATTAAGTGATAGAGAGAAGAAAATTGCACATCTTGCTGTGTTAATGAATACAGTTGTGGTGGATCCATATTTAGACATACACTTGGAGGAGTTACAAGAGTCAAACGAACGCCTCAGAAATGATGCAACCTTACTTTGGAATAGACATACTGAAAAATTTGCAGAATGGATAAAAAAGAAGGTTCCTATTGATTCAAATAATCACTCGGAGACATTGAAATGGATTGCATATGGTCCACGGAGTAGTGCGTTGTCTTTTACTGGGTATATTGTCAACGGGCAGCGGTTTCACACAAAAAATATTGACAGAGAAACGCAAAACAGTGGGGTGACTTATGATGCAACTAGCATGTGTAGGGCAAGCGCAAAAGATACTGCACAAGTGCCTGATTTGGCACCACCAAGAGGTTACCATGAGCCAAAGGTCGACGATGAAAATGCAGATGTAGCCTCAATGCCACCAGAAATTGACATGGATATTGACAAGTCTAGTGATGAAATTAGATATGTCAGGGAGGATTGTGAAGGCATATTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transpos_assoc Transposase_21

Protein Analysis

403

Amino Acids

45.73

Weight (kDa)

5.41

Isoelectric Point (pI)

35.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transpos_assoc PF13963 60 - 124 1.1e-13 Transposase-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1066
AccB7I CCANNNNNTGG 1 cut(s) 653
AccI GTMKAC 1 cut(s) 1098
AciI CCGC 1 cut(s) 955
AclWI GGATC 5 cut(s) 166, 194, 485, 728, 741
AcsI RAATTY 4 cut(s) 72, 226, 430, 827
AcvI CACGTG 1 cut(s) 586
AfaI GTAC 2 cut(s) 209, 365
AfiI CCNNNNNNNGG 3 cut(s) 653, 807, 1076
AflIII ACRYGT 1 cut(s) 583
AgsI TTSAA 4 cut(s) 147, 332, 867, 889
AjnI CCWGG 1 cut(s) 252
AluBI AGCT 6 cut(s) 16, 104, 124, 173, 197, 553
AluI AGCT 6 cut(s) 16, 104, 124, 173, 197, 553
Alw26I GTCTC 1 cut(s) 875
AlwI GGATC 5 cut(s) 166, 194, 485, 728, 741
Ama87I CYCGRG 1 cut(s) 203
AoxI GGCC 1 cut(s) 357
ApeKI GCWGC 3 cut(s) 8, 16, 952
ApoI RAATTY 4 cut(s) 72, 226, 430, 827
Asp700I GAANNNNTTC 1 cut(s) 630
AspLEI GCGC 1 cut(s) 1037
AspS9I GGNCC 1 cut(s) 905
AsuHPI GGTGA 2 cut(s) 518, 1012
AvaI CYCGRG 1 cut(s) 203
AvaII GGWCC 1 cut(s) 905
BamHI GGATCC 1 cut(s) 733
BanI GGYRCC 1 cut(s) 1066
BbrPI CACGTG 1 cut(s) 586
BbsI GAAGAC 2 cut(s) 179, 516
BbvI GCAGC 3 cut(s) 3, 20, 964
BccI CCATC 1 cut(s) 497
BcgI CGANNNNNNTGC 2 cut(s) 1091, 1125
BciT130I CCWGG 1 cut(s) 254
BclI TGATCA 1 cut(s) 541
BcoDI GTCTC 1 cut(s) 875
BfaI CTAG 3 cut(s) 99, 1017, 1161
BisI GCNGC 3 cut(s) 9, 17, 953
BlpI GCTNAGC 1 cut(s) 120
BlsI GCNGC 3 cut(s) 10, 18, 954
Bme1390I CCNGG 1 cut(s) 254
Bme18I GGWCC 1 cut(s) 905
BmeT110I CYCGRG 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 905
BmiI GGNNCC 5 cut(s) 251, 658, 735, 855, 1068
BmrFI CCNGG 1 cut(s) 254
BmrI ACTGGG 1 cut(s) 942
BmsI GCATC 3 cut(s) 16, 784, 1000
BmuI ACTGGG 1 cut(s) 942
BpiI GAAGAC 2 cut(s) 179, 516
BpmI CTGGAG 1 cut(s) 675
Bpu1102I GCTNAGC 1 cut(s) 120
Bsa29I ATCGAT 1 cut(s) 665
BsaAI YACGTR 1 cut(s) 586
BsaJI CCNNGG 2 cut(s) 253, 908
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bsc4I CCNNNNNNNGG 3 cut(s) 653, 807, 1076
Bse1I ACTGG 2 cut(s) 50, 937
Bse3DI GCAATG 3 cut(s) 304, 613, 628
BseBI CCWGG 1 cut(s) 254
BseCI ATCGAT 1 cut(s) 665
BseDI CCNNGG 2 cut(s) 253, 908
BseGI GGATG 1 cut(s) 38
BseLI CCNNNNNNNGG 3 cut(s) 653, 807, 1076
BseMI GCAATG 3 cut(s) 304, 613, 628
BseMII CTCAG 2 cut(s) 111, 799
BseNI ACTGG 2 cut(s) 50, 937
BseRI GAGGAG 3 cut(s) 98, 563, 773
BseXI GCAGC 3 cut(s) 3, 20, 964
BsgI GTGCAG 1 cut(s) 1032
BshFI GGCC 1 cut(s) 359
BshNI GGYRCC 1 cut(s) 1066
BshVI ATCGAT 1 cut(s) 665
BsiHKCI CYCGRG 1 cut(s) 203
BslI CCNNNNNNNGG 3 cut(s) 653, 807, 1076
BsmAI GTCTC 1 cut(s) 875
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 1 cut(s) 359
BsoBI CYCGRG 1 cut(s) 203
Bsp143I GATC 8 cut(s) 158, 199, 270, 304, 490, 541, 666, 733
Bsp1720I GCTNAGC 1 cut(s) 120
BspACI CCGC 1 cut(s) 955
BspANI GGCC 1 cut(s) 359
BspCNI CTCAG 2 cut(s) 112, 798
BspDI ATCGAT 1 cut(s) 665
BspLI GGNNCC 5 cut(s) 251, 658, 735, 855, 1068
BspPI GGATC 5 cut(s) 166, 194, 485, 728, 741
BspT107I GGYRCC 1 cut(s) 1066
BsrDI GCAATG 3 cut(s) 304, 613, 628
BsrI ACTGG 2 cut(s) 50, 937
BssECI CCNNGG 2 cut(s) 253, 908
BssMI GATC 8 cut(s) 158, 199, 270, 304, 490, 541, 666, 733
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 3 cut(s) 241, 724, 995
BstAPI GCANNNNNTGC 1 cut(s) 704
BstBAI YACGTR 1 cut(s) 586
BstC8I GCNNGC 1 cut(s) 1033
BstDEI CTNAG 3 cut(s) 120, 425, 785
BstDSI CCRYGG 1 cut(s) 908
BstEII GGTNACC 1 cut(s) 1079
BstF5I GGATG 1 cut(s) 38
BstHHI GCGC 1 cut(s) 1037
BstKTI GATC 8 cut(s) 161, 202, 273, 307, 493, 544, 669, 736
BstMAI GTCTC 1 cut(s) 875
BstMBI GATC 8 cut(s) 158, 199, 270, 304, 490, 541, 666, 733
BstMWI GCNNNNNNNGC 2 cut(s) 704, 1118
BstNI CCWGG 1 cut(s) 254
BstNSI RCATGY 1 cut(s) 1024
BstPI GGTNACC 1 cut(s) 1079
BstSCI CCNGG 1 cut(s) 252
BstV1I GCAGC 3 cut(s) 3, 20, 964
BstV2I GAAGAC 2 cut(s) 179, 516
BstX2I RGATCY 1 cut(s) 733
BstYI RGATCY 1 cut(s) 733
Bsu15I ATCGAT 1 cut(s) 665
BsuRI GGCC 1 cut(s) 359
BsuTUI ATCGAT 1 cut(s) 665
BtgI CCRYGG 1 cut(s) 908
BtsCI GGATG 1 cut(s) 38
BtsIMutI CAGTG 1 cut(s) 1000
Cac8I GCNNGC 1 cut(s) 1033
CfoI GCGC 1 cut(s) 1037
Cfr13I GGNCC 1 cut(s) 905
ClaI ATCGAT 1 cut(s) 665
CseI GACGC 1 cut(s) 173
Csp6I GTAC 2 cut(s) 208, 364
CspCI CAANNNNNGTGG 2 cut(s) 567, 602
CviAII CATG 6 cut(s) 95, 222, 314, 1021, 1085, 1144
CviQI GTAC 2 cut(s) 208, 364
DdeI CTNAG 3 cut(s) 120, 425, 785
DpnI GATC 8 cut(s) 160, 201, 272, 306, 492, 543, 668, 735
DpnII GATC 8 cut(s) 158, 199, 270, 304, 490, 541, 666, 733
Eco47I GGWCC 1 cut(s) 905
Eco72I CACGTG 1 cut(s) 586
Eco88I CYCGRG 1 cut(s) 203
Eco91I GGTNACC 1 cut(s) 1079
EcoO65I GGTNACC 1 cut(s) 1079
EcoRII CCWGG 1 cut(s) 252
FaeI CATG 6 cut(s) 98, 225, 317, 1024, 1088, 1147
FatI CATG 6 cut(s) 94, 221, 313, 1020, 1084, 1143
FauNDI CATATG 1 cut(s) 901
FbaI TGATCA 1 cut(s) 541
FblI GTMKAC 1 cut(s) 1098
Fnu4HI GCNGC 3 cut(s) 9, 17, 953
FokI GGATG 1 cut(s) 25
Fsp4HI GCNGC 3 cut(s) 9, 17, 953
FspBI CTAG 3 cut(s) 99, 1017, 1161
GlaI GCGC 1 cut(s) 1036
GluI GCNGC 3 cut(s) 9, 17, 953
GsuI CTGGAG 1 cut(s) 675
HaeIII GGCC 1 cut(s) 359
HgaI GACGC 1 cut(s) 173
HhaI GCGC 1 cut(s) 1037
Hin1II CATG 6 cut(s) 98, 225, 317, 1024, 1088, 1147
Hin6I GCGC 1 cut(s) 1035
HinP1I GCGC 1 cut(s) 1035
HincII GTYRAC 2 cut(s) 946, 1099
HindII GTYRAC 2 cut(s) 946, 1099
HinfI GANTC 4 cut(s) 393, 631, 770, 863
HphI GGTGA 2 cut(s) 518, 1012
Hpy166II GTNNAC 3 cut(s) 908, 946, 1099
Hpy188I TCNGA 6 cut(s) 43, 408, 502, 570, 788, 880
Hpy188III TCNNGA 4 cut(s) 203, 397, 545, 654
Hpy8I GTNNAC 3 cut(s) 908, 946, 1099
Hpy99I CGWCG 1 cut(s) 1103
HpyAV CCTTC 2 cut(s) 844, 1190
HpyCH4III ACNGT 3 cut(s) 241, 724, 995
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 9 cut(s) 8, 285, 698, 797, 833, 899, 1013, 1049, 1112
HpyF10VI GCNNNNNNNGC 2 cut(s) 704, 1118
HpyF3I CTNAG 3 cut(s) 120, 425, 785
HpySE526I ACGT 1 cut(s) 585
Hsp92II CATG 6 cut(s) 98, 225, 317, 1024, 1088, 1147
HspAI GCGC 1 cut(s) 1035
Ksp22I TGATCA 1 cut(s) 541
Kzo9I GATC 8 cut(s) 158, 199, 270, 304, 490, 541, 666, 733
LmnI GCTCC 2 cut(s) 550, 656
Lsp1109I GCAGC 3 cut(s) 3, 20, 964
LweI GCATC 3 cut(s) 16, 784, 1000
MaeI CTAG 3 cut(s) 99, 1017, 1161
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 5 cut(s) 340, 458, 762, 1000, 1079
MalI GATC 8 cut(s) 160, 201, 272, 306, 492, 543, 668, 735
MboI GATC 8 cut(s) 158, 199, 270, 304, 490, 541, 666, 733
MboII GAAGA 3 cut(s) 179, 521, 700
MflI RGATCY 1 cut(s) 733
MluCI AATT 8 cut(s) 72, 226, 430, 648, 693, 827, 1137, 1170
MlyI GAGTC 1 cut(s) 779
MmeI TCCRAC 2 cut(s) 83, 386
MroXI GAANNNNTTC 1 cut(s) 630
MseI TTAA 4 cut(s) 90, 525, 674, 713
MslI CAYNNNNRTG 3 cut(s) 312, 889, 1205
MspA1I CMGCKG 1 cut(s) 955
MspR9I CCNGG 1 cut(s) 254
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 1 cut(s) 254
MwoI GCNNNNNNNGC 2 cut(s) 704, 1118
NdeI CATATG 1 cut(s) 901
NdeII GATC 8 cut(s) 158, 199, 270, 304, 490, 541, 666, 733
NlaIII CATG 6 cut(s) 98, 225, 317, 1024, 1088, 1147
NlaIV GGNNCC 5 cut(s) 251, 658, 735, 855, 1068
NmuCI GTSAC 1 cut(s) 1000
NspI RCATGY 1 cut(s) 1024
PctI GAATGC 1 cut(s) 10
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 3 cut(s) 393, 631, 863
PflMI CCANNNNNTGG 1 cut(s) 653
PkrI GCNGC 3 cut(s) 10, 18, 954
PleI GAGTC 1 cut(s) 778
PmaCI CACGTG 1 cut(s) 586
PmlI CACGTG 1 cut(s) 586
PpsI GAGTC 1 cut(s) 778
Ppu21I YACGTR 1 cut(s) 586
Psp6I CCWGG 1 cut(s) 252
PspCI CACGTG 1 cut(s) 586
PspEI GGTNACC 1 cut(s) 1079
PspGI CCWGG 1 cut(s) 252
PspN4I GGNNCC 5 cut(s) 251, 658, 735, 855, 1068
PspPI GGNCC 1 cut(s) 905
PsuI RGATCY 1 cut(s) 733
RsaI GTAC 2 cut(s) 209, 365
RsaNI GTAC 2 cut(s) 208, 364
RseI CAYNNNNRTG 3 cut(s) 312, 889, 1205
SalI GTCGAC 1 cut(s) 1097
SaqAI TTAA 4 cut(s) 90, 525, 674, 713
SatI GCNGC 3 cut(s) 9, 17, 953
Sau3AI GATC 8 cut(s) 158, 199, 270, 304, 490, 541, 666, 733
Sau96I GGNCC 1 cut(s) 905
SchI GAGTC 1 cut(s) 779
ScrFI CCNGG 1 cut(s) 254
SfaNI GCATC 3 cut(s) 16, 784, 1000
SinI GGWCC 1 cut(s) 905
SmiMI CAYNNNNRTG 3 cut(s) 312, 889, 1205
Sse9I AATT 8 cut(s) 72, 226, 430, 648, 693, 827, 1137, 1170
SsiI CCGC 1 cut(s) 955
SspI AATATT 3 cut(s) 322, 412, 973
SspMI CTAG 3 cut(s) 99, 1017, 1161
StyD4I CCNGG 1 cut(s) 252
TaaI ACNGT 3 cut(s) 241, 724, 995
TaiI ACGT 1 cut(s) 588
TaqI TCGA 2 cut(s) 665, 1098
TasI AATT 8 cut(s) 72, 226, 430, 648, 693, 827, 1137, 1170
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 3 cut(s) 393, 631, 863
Tru1I TTAA 4 cut(s) 90, 525, 674, 713
Tru9I TTAA 4 cut(s) 90, 525, 674, 713
TscAI CASTG 1 cut(s) 1000
TseFI GTSAC 1 cut(s) 1000
TseI GCWGC 3 cut(s) 8, 16, 952
Tsp45I GTSAC 1 cut(s) 1000
TspDTI ATGAA 5 cut(s) 17, 639, 731, 1119, 1182
TspGWI ACGGA 1 cut(s) 925
TspRI CASTG 1 cut(s) 1000
Van91I CCANNNNNTGG 1 cut(s) 653
VpaK11BI GGWCC 1 cut(s) 905
XapI RAATTY 4 cut(s) 72, 226, 430, 827
XceI RCATGY 1 cut(s) 1024
XcmI CCANNNNNNNNNTGG 1 cut(s) 101
XmiI GTMKAC 1 cut(s) 1098
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 3 cut(s) 99, 1017, 1161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.