Rh4AG198500

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0013781
Physical Location & Seq
Forward (+)
48281687 .. 48282094
408 bp
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UTR
Exon/CDS
Intron
Rh4AG198500.1

Sequence Viewer

Length: 408 bp
ATGATCTTATGTCCTTGCATAGATTGTCGCAATGTAGATCATCAGTATGGCACTATTGTTGTTGAATATTTGGTTACAAGGGGAATTGAGGCCAAGTACAAACAAAGAAAAGATTGGTATGAGCATGGGGAGCAAATAATTAATGGTCATAAAAATGAGGAAGTGGTTAATGATGAGATATATGAGTTATTTAGAGCTGCCCAATTTTTGGATCAAGACTACATCAAGCCTACTGAGTTTGTTAATGAAGATTGTGTTGAGCCCACTGAAGACATACATGATGATGATTTCATAGAAAAACTAGAAGATGCAGAAACTCCTTTGTACCCGAATTGTTCAAAGTACAACAAGTTATCAGCCATTGTAGCTTTATATCGACTTAAAACTCAGAGTGGGTGGTCTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transpos_assoc Transposase_21

Protein Analysis

135

Amino Acids

15.89

Weight (kDa)

4.56

Isoelectric Point (pI)

29.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transpos_assoc PF13963 2 - 44 7.4e-08 Transposase-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 208
AclWI GGATC 1 cut(s) 219
AcuI CTGAAG 1 cut(s) 288
AfaI GTAC 3 cut(s) 98, 326, 344
AfiI CCNNNNNNNGG 1 cut(s) 208
AgsI TTSAA 2 cut(s) 65, 339
AluBI AGCT 2 cut(s) 197, 368
AluI AGCT 2 cut(s) 197, 368
AlwI GGATC 1 cut(s) 219
AoxI GGCC 1 cut(s) 90
ApeKI GCWGC 1 cut(s) 197
AseI ATTAAT 1 cut(s) 141
BanII GRGCYC 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 276
BbvI GCAGC 1 cut(s) 184
BfaI CTAG 1 cut(s) 302
BisI GCNGC 1 cut(s) 198
BlsI GCNGC 1 cut(s) 199
BmsI GCATC 1 cut(s) 298
BpiI GAAGAC 1 cut(s) 276
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse3DI GCAATG 1 cut(s) 37
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 2 cut(s) 225, 401
BseXI GCAGC 1 cut(s) 184
BshFI GGCC 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 208
BsnI GGCC 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 264
Bsp143I GATC 3 cut(s) 3, 37, 211
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 2 cut(s) 226, 400
BspPI GGATC 1 cut(s) 219
BsrDI GCAATG 1 cut(s) 37
BssMI GATC 3 cut(s) 3, 37, 211
BstDEI CTNAG 2 cut(s) 234, 387
BstKTI GATC 3 cut(s) 6, 40, 214
BstMBI GATC 3 cut(s) 3, 37, 211
BstMWI GCNNNNNNNGC 2 cut(s) 130, 365
BstV1I GCAGC 1 cut(s) 184
BstV2I GAAGAC 1 cut(s) 276
BsuRI GGCC 1 cut(s) 92
BtsIMutI CAGTG 1 cut(s) 264
Csp6I GTAC 3 cut(s) 97, 325, 343
CviAII CATG 2 cut(s) 125, 278
CviJI RGCY 6 cut(s) 92, 197, 229, 262, 359, 368
CviKI_1 RGCY 6 cut(s) 92, 197, 229, 262, 359, 368
CviQI GTAC 3 cut(s) 97, 325, 343
DdeI CTNAG 2 cut(s) 234, 387
DpnI GATC 3 cut(s) 5, 39, 213
DpnII GATC 3 cut(s) 3, 37, 211
Eco24I GRGCYC 1 cut(s) 264
Eco57I CTGAAG 1 cut(s) 288
EcoT38I GRGCYC 1 cut(s) 264
FaeI CATG 2 cut(s) 128, 281
FatI CATG 2 cut(s) 124, 277
Fnu4HI GCNGC 1 cut(s) 198
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 1 cut(s) 198
FspBI CTAG 1 cut(s) 302
GluI GCNGC 1 cut(s) 198
HaeIII GGCC 1 cut(s) 92
Hin1II CATG 2 cut(s) 128, 281
Hpy188I TCNGA 2 cut(s) 390, 403
Hpy188III TCNNGA 1 cut(s) 215
HpyCH4V TGCA 2 cut(s) 18, 311
HpyF10VI GCNNNNNNNGC 2 cut(s) 130, 365
HpyF3I CTNAG 2 cut(s) 234, 387
Hsp92II CATG 2 cut(s) 128, 281
Kzo9I GATC 3 cut(s) 3, 37, 211
LmnI GCTCC 1 cut(s) 130
Lsp1109I GCAGC 1 cut(s) 184
LweI GCATC 1 cut(s) 298
MaeI CTAG 1 cut(s) 302
MaeIII GTNAC 1 cut(s) 73
MalI GATC 3 cut(s) 5, 39, 213
MboI GATC 3 cut(s) 3, 37, 211
MboII GAAGA 3 cut(s) 260, 281, 317
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 4 cut(s) 84, 138, 203, 331
MnlI CCTC 2 cut(s) 82, 151
MseI TTAA 4 cut(s) 141, 168, 243, 381
MslI CAYNNNNRTG 3 cut(s) 45, 153, 282
MwoI GCNNNNNNNGC 2 cut(s) 130, 365
NdeII GATC 3 cut(s) 3, 37, 211
NlaIII CATG 2 cut(s) 128, 281
PflMI CCANNNNNTGG 1 cut(s) 208
PkrI GCNGC 1 cut(s) 199
PshBI ATTAAT 1 cut(s) 141
RsaI GTAC 3 cut(s) 98, 326, 344
RsaNI GTAC 3 cut(s) 97, 325, 343
RseI CAYNNNNRTG 3 cut(s) 45, 153, 282
SaqAI TTAA 4 cut(s) 141, 168, 243, 381
SatI GCNGC 1 cut(s) 198
Sau3AI GATC 3 cut(s) 3, 37, 211
SduI GDGCHC 1 cut(s) 264
SetI ASST 2 cut(s) 199, 370
SfaNI GCATC 1 cut(s) 298
SmiMI CAYNNNNRTG 3 cut(s) 45, 153, 282
Sse9I AATT 4 cut(s) 84, 138, 203, 331
SspI AATATT 1 cut(s) 68
SspMI CTAG 1 cut(s) 302
TaqI TCGA 1 cut(s) 376
TasI AATT 4 cut(s) 84, 138, 203, 331
TatI WGTACW 2 cut(s) 96, 342
Tru1I TTAA 4 cut(s) 141, 168, 243, 381
Tru9I TTAA 4 cut(s) 141, 168, 243, 381
TscAI CASTG 1 cut(s) 271
TseI GCWGC 1 cut(s) 197
TspDTI ATGAA 2 cut(s) 261, 280
TspRI CASTG 1 cut(s) 271
Van91I CCANNNNNTGG 1 cut(s) 208
VspI ATTAAT 1 cut(s) 141
XspI CTAG 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.