Rh2AG300200

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0005938
Physical Location & Seq
Reverse (-)
37520162 .. 37521614
1453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG300200.1

Sequence Viewer

Length: 507 bp
ATGCAGATGGACCTTGGGCTCTTCGACGACGGCGGAGATGAGATCAGAGAGGAGATCGAAGCAGAGGAGAGGCTCCGTCGTCCTCCTAATCTGGCCCTCTCCTCCACTGGGCTCACCTCACCGCCACCACTTTTCTCTCTCTCCACAGAGCCTTCCCGCCGACCCACCACCGAGTCCAACTTGATCGCCGCCGCAGCCCATGTCCTCCACCGGCCTCCAAGAGGACATGGGCGACAGTTCCATCTTTTTGCAATGAATAAACACATGGAGCAATATGTGAACAAGATAATGAAGATGATGAAGGATGAAAAGCTATTTGCTCCACAAGGAGGTCCAATCATCTTAGCTCAGATTGAAAATGAGTACAATCAAATCCAACTTGCATATAGAGAGTTGGGAGATACATATGTGCAGTGGGCAGCAAAGCTGGCAGTTGGGCAGAATATTGGAGTGCCATGGATCATGTGCAAGCAAAAGGATGCTCCTGATCCAGTGGTAAGTAATTGA

Protein Analysis

168

Amino Acids

18.92

Weight (kDa)

5.95

Isoelectric Point (pI)

58.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 86 - 165 3.1e-17 Glycosyl hydrolases family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 33, 122, 157, 189, 192
AclWI GGATC 2 cut(s) 467, 482
AfaI GTAC 1 cut(s) 365
AfiI CCNNNNNNNGG 3 cut(s) 108, 221, 329
AgsI TTSAA 1 cut(s) 356
AluBI AGCT 3 cut(s) 313, 347, 427
AluI AGCT 3 cut(s) 313, 347, 427
AlwI GGATC 2 cut(s) 467, 482
AoxI GGCC 2 cut(s) 93, 212
ApeKI GCWGC 2 cut(s) 194, 419
AspS9I GGNCC 3 cut(s) 10, 94, 332
AsuHPI GGTGA 2 cut(s) 106, 111
AvaII GGWCC 2 cut(s) 10, 332
BaeI ACNNNNGTAYC 2 cut(s) 393, 426
BanII GRGCYC 2 cut(s) 21, 114
BbvI GCAGC 2 cut(s) 206, 431
BccI CCATC 1 cut(s) 249
BceAI ACGGC 1 cut(s) 46
BisI GCNGC 4 cut(s) 189, 192, 195, 420
BlsI GCNGC 4 cut(s) 190, 193, 196, 421
Bme18I GGWCC 2 cut(s) 10, 332
BmgT120I GGNCC 3 cut(s) 10, 94, 332
BmiI GGNNCC 1 cut(s) 74
BmrI ACTGGG 1 cut(s) 117
BmsI GCATC 1 cut(s) 469
BmuI ACTGGG 1 cut(s) 117
BsaJI CCNNGG 2 cut(s) 13, 455
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 221, 329
Bse118I RCCGGY 1 cut(s) 210
Bse1I ACTGG 2 cut(s) 112, 491
Bse3DI GCAATG 1 cut(s) 258
BseDI CCNNGG 2 cut(s) 13, 455
BseGI GGATG 2 cut(s) 310, 484
BseLI CCNNNNNNNGG 3 cut(s) 108, 221, 329
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 1 cut(s) 362
BseNI ACTGG 2 cut(s) 112, 491
BseRI GAGGAG 3 cut(s) 65, 80, 91
BseXI GCAGC 2 cut(s) 206, 431
BsgI GTGCAG 1 cut(s) 431
BshFI GGCC 2 cut(s) 95, 214
BsiSI CCGG 1 cut(s) 211
BslI CCNNNNNNNGG 3 cut(s) 108, 221, 329
BsnI GGCC 2 cut(s) 95, 214
Bsp1286I GDGCHC 2 cut(s) 21, 114
Bsp143I GATC 5 cut(s) 42, 54, 183, 459, 487
Bsp19I CCATGG 1 cut(s) 455
BspACI CCGC 5 cut(s) 33, 122, 157, 189, 192
BspANI GGCC 2 cut(s) 95, 214
BspCNI CTCAG 1 cut(s) 361
BspLI GGNNCC 1 cut(s) 74
BspPI GGATC 2 cut(s) 467, 482
BspQI GCTCTTC 1 cut(s) 26
BsrDI GCAATG 1 cut(s) 258
BsrFI RCCGGY 1 cut(s) 210
BsrI ACTGG 2 cut(s) 112, 491
BssAI RCCGGY 1 cut(s) 210
BssECI CCNNGG 2 cut(s) 13, 455
BssMI GATC 5 cut(s) 42, 54, 183, 459, 487
BssT1I CCWWGG 2 cut(s) 13, 455
Bst4CI ACNGT 1 cut(s) 237
Bst6I CTCTTC 1 cut(s) 26
BstC8I GCNNGC 2 cut(s) 429, 470
BstDEI CTNAG 2 cut(s) 343, 348
BstDSI CCRYGG 1 cut(s) 455
BstENI CCTNNNNNAGG 1 cut(s) 219
BstF5I GGATG 2 cut(s) 310, 484
BstKTI GATC 5 cut(s) 45, 57, 186, 462, 490
BstMBI GATC 5 cut(s) 42, 54, 183, 459, 487
BstMWI GCNNNNNNNGC 2 cut(s) 194, 428
BstV1I GCAGC 2 cut(s) 206, 431
BsuRI GGCC 2 cut(s) 95, 214
BtgI CCRYGG 1 cut(s) 455
BtsCI GGATG 2 cut(s) 310, 484
BtsI GCAGTG 1 cut(s) 419
BtsIMutI CAGTG 3 cut(s) 105, 419, 498
Cac8I GCNNGC 2 cut(s) 429, 470
Cfr10I RCCGGY 1 cut(s) 210
Cfr13I GGNCC 3 cut(s) 10, 94, 332
Csp6I GTAC 1 cut(s) 364
CviAII CATG 5 cut(s) 200, 227, 265, 456, 463
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 2 cut(s) 343, 348
DpnI GATC 5 cut(s) 44, 56, 185, 461, 489
DpnII GATC 5 cut(s) 42, 54, 183, 459, 487
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EciI GGCGGA 1 cut(s) 48
Eco130I CCWWGG 2 cut(s) 13, 455
Eco24I GRGCYC 2 cut(s) 21, 114
Eco47I GGWCC 2 cut(s) 10, 332
EcoNI CCTNNNNNAGG 1 cut(s) 219
EcoT14I CCWWGG 2 cut(s) 13, 455
EcoT38I GRGCYC 2 cut(s) 21, 114
ErhI CCWWGG 2 cut(s) 13, 455
FaeI CATG 5 cut(s) 203, 230, 268, 459, 466
FatI CATG 5 cut(s) 199, 226, 264, 455, 462
FauI CCCGC 1 cut(s) 164
FauNDI CATATG 1 cut(s) 406
Fnu4HI GCNGC 4 cut(s) 189, 192, 195, 420
FokI GGATG 2 cut(s) 317, 491
FriOI GRGCYC 2 cut(s) 21, 114
Fsp4HI GCNGC 4 cut(s) 189, 192, 195, 420
GluI GCNGC 4 cut(s) 189, 192, 195, 420
HaeIII GGCC 2 cut(s) 95, 214
HapII CCGG 1 cut(s) 211
Hin1II CATG 5 cut(s) 203, 230, 268, 459, 466
HinfI GANTC 1 cut(s) 173
HpaII CCGG 1 cut(s) 211
HphI GGTGA 2 cut(s) 106, 111
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 2 cut(s) 47, 351
Hpy188III TCNNGA 1 cut(s) 485
Hpy8I GTNNAC 1 cut(s) 280
Hpy99I CGWCG 3 cut(s) 29, 32, 81
HpyAV CCTTC 2 cut(s) 162, 295
HpyCH4III ACNGT 1 cut(s) 237
HpyCH4V TGCA 5 cut(s) 4, 251, 383, 412, 468
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 428
HpyF3I CTNAG 2 cut(s) 343, 348
Hsp92II CATG 5 cut(s) 203, 230, 268, 459, 466
Kzo9I GATC 5 cut(s) 42, 54, 183, 459, 487
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 4 cut(s) 78, 268, 325, 487
LpnPI CCDG 5 cut(s) 77, 93, 224, 413, 498
Lsp1109I GCAGC 2 cut(s) 206, 431
LweI GCATC 1 cut(s) 469
MalI GATC 5 cut(s) 44, 56, 185, 461, 489
MboI GATC 5 cut(s) 42, 54, 183, 459, 487
MboII GAAGA 2 cut(s) 13, 304
MhlI GDGCHC 2 cut(s) 21, 114
MluCI AATT 1 cut(s) 502
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 2 cut(s) 201, 400
MspI CCGG 1 cut(s) 211
MwoI GCNNNNNNNGC 2 cut(s) 194, 428
NcoI CCATGG 1 cut(s) 455
NdeI CATATG 1 cut(s) 406
NdeII GATC 5 cut(s) 42, 54, 183, 459, 487
NlaIII CATG 5 cut(s) 203, 230, 268, 459, 466
NlaIV GGNNCC 1 cut(s) 74
PciSI GCTCTTC 1 cut(s) 26
PkrI GCNGC 4 cut(s) 190, 193, 196, 421
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
PspN4I GGNNCC 1 cut(s) 74
PspPI GGNCC 3 cut(s) 10, 94, 332
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
SapI GCTCTTC 1 cut(s) 26
SatI GCNGC 4 cut(s) 189, 192, 195, 420
Sau3AI GATC 5 cut(s) 42, 54, 183, 459, 487
Sau96I GGNCC 3 cut(s) 10, 94, 332
SchI GAGTC 1 cut(s) 182
SduI GDGCHC 2 cut(s) 21, 114
SetI ASST 6 cut(s) 15, 119, 315, 334, 349, 429
SfaNI GCATC 1 cut(s) 469
SinI GGWCC 2 cut(s) 10, 332
Sse9I AATT 1 cut(s) 502
SsiI CCGC 5 cut(s) 33, 122, 157, 189, 192
SspI AATATT 1 cut(s) 445
StyI CCWWGG 2 cut(s) 13, 455
TaaI ACNGT 1 cut(s) 237
TaqI TCGA 2 cut(s) 24, 57
TasI AATT 1 cut(s) 502
TatI WGTACW 1 cut(s) 363
TauI GCSGC 2 cut(s) 191, 194
TscAI CASTG 3 cut(s) 112, 419, 498
TseI GCWGC 2 cut(s) 194, 419
TspDTI ATGAA 4 cut(s) 269, 305, 314, 321
TspGWI ACGGA 1 cut(s) 65
TspRI CASTG 3 cut(s) 112, 419, 498
VpaK11BI GGWCC 2 cut(s) 10, 332
XagI CCTNNNNNAGG 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.