Rh5DG196500

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005938
Physical Location & Seq
Reverse (-)
21818559 .. 21820885
2327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG196500.1

Sequence Viewer

Length: 804 bp
ATGCAGATGGACCTTGGGCTCTTCGACGACGGCGGAGATGAGATCAGAGAGGAGATCGAAGCAGAGGAGAGGCTCCGTCGTCCTCCTAATCTGGCCCTCTCCTCCACTGGGCTCACCTCACCGCCACCACTTTTCTCTCTCTCCACAGAGCCTTCCCGCCGACCCACCACCGAGTCCAACTCGATCGCCGCCGCAGCCCATGTCCTCCACGGGCCTCCAAGAGGACATGGGCAACAGTTCCATCTTTTTGCAATGAATGTTATGCTTGATTATCTGTGTTGTTTCAAATTTGGGCAGAAAAGAAGAACTGATAAGCAAGGAGTCACTTTCCGATCAGTTAATGAACCATACAAGAAACACATGGAGCAATATGTGAACAAGATAATGAAGATGATGAAGGATGAAAAGCTATTTGCTCCACAAGGAGGTCCAATCATCTTAGCTCAGATTGAAAATGAGTACAATCAAATCCAACTTGCATATAGAGAGTTGGGAGATACATATGTGCAGTGGGCAGCAAAGCTAGCAGTTGGGCAGAATATTAGAGTGCCATGGATCATGTGCAAGCAAAAGGATGCTCCTGATCCAGTGATTAATACTTGCAACGGAAGGCATTGTGGAGATACTTTTTCAGGACCTAACAAACCCAACAAGCCTGCTCTATGGACTGAGAATTGGACAGCTCACTTTGCAAAGGGACCCGAAATGGAGTCACCTCAAGGACTTGCACAAGGCTATAAATCTATGCAAGAAAACTTTGCTTACTGGAACTCCCGGAGACCAAAGGTTGGGGGAAGAAACTGA

Protein Analysis

267

Amino Acids

30.26

Weight (kDa)

8.73

Isoelectric Point (pI)

52.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 104 - 232 1e-30 Glycosyl hydrolases family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 788
AciI CCGC 5 cut(s) 33, 122, 157, 189, 192
AclWI GGATC 2 cut(s) 563, 578
AcsI RAATTY 1 cut(s) 287
AfaI GTAC 1 cut(s) 461
AfiI CCNNNNNNNGG 4 cut(s) 108, 221, 425, 788
AgsI TTSAA 2 cut(s) 286, 452
AluBI AGCT 4 cut(s) 409, 443, 523, 683
AluI AGCT 4 cut(s) 409, 443, 523, 683
Alw26I GTCTC 1 cut(s) 772
AlwI GGATC 2 cut(s) 563, 578
AoxI GGCC 2 cut(s) 93, 212
ApeKI GCWGC 2 cut(s) 194, 515
ApoI RAATTY 1 cut(s) 287
AseI ATTAAT 1 cut(s) 594
AspS9I GGNCC 6 cut(s) 10, 94, 212, 428, 635, 698
AsuC2I CCSGG 1 cut(s) 775
AsuHPI GGTGA 3 cut(s) 106, 111, 705
AsuNHI GCTAGC 1 cut(s) 523
AvaII GGWCC 4 cut(s) 10, 428, 635, 698
BaeI ACNNNNGTAYC 2 cut(s) 489, 522
BanII GRGCYC 2 cut(s) 21, 114
BbvI GCAGC 2 cut(s) 206, 527
BccI CCATC 1 cut(s) 249
BceAI ACGGC 1 cut(s) 46
BcnI CCSGG 1 cut(s) 775
BcoDI GTCTC 1 cut(s) 772
BfaI CTAG 1 cut(s) 524
BisI GCNGC 4 cut(s) 189, 192, 195, 516
BlsI GCNGC 4 cut(s) 190, 193, 196, 517
Bme1390I CCNGG 1 cut(s) 775
Bme18I GGWCC 4 cut(s) 10, 428, 635, 698
BmgT120I GGNCC 6 cut(s) 10, 94, 212, 428, 635, 698
BmiI GGNNCC 3 cut(s) 74, 699, 700
BmrFI CCNGG 1 cut(s) 775
BmrI ACTGGG 1 cut(s) 117
BmsI GCATC 1 cut(s) 565
BmtI GCTAGC 1 cut(s) 527
BmuI ACTGGG 1 cut(s) 117
BplI GAGNNNNNCTC 2 cut(s) 164, 196
BpuEI CTTGAG 1 cut(s) 702
BpuMI CCSGG 1 cut(s) 775
BsaI GGTCTC 1 cut(s) 772
BsaJI CCNNGG 3 cut(s) 13, 208, 551
BsaXI ACNNNNNCTCC 2 cut(s) 755, 785
Bsc4I CCNNNNNNNGG 4 cut(s) 108, 221, 425, 788
Bse1I ACTGG 3 cut(s) 112, 587, 770
Bse3DI GCAATG 1 cut(s) 258
BseDI CCNNGG 3 cut(s) 13, 208, 551
BseGI GGATG 2 cut(s) 406, 580
BseLI CCNNNNNNNGG 4 cut(s) 108, 221, 425, 788
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 2 cut(s) 458, 660
BseNI ACTGG 3 cut(s) 112, 587, 770
BseRI GAGGAG 3 cut(s) 65, 80, 91
BseXI GCAGC 2 cut(s) 206, 527
BsgI GTGCAG 1 cut(s) 527
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 2 cut(s) 95, 214
BsiEI CGRYCG 1 cut(s) 186
BsiSI CCGG 1 cut(s) 775
BslFI GGGAC 1 cut(s) 711
BslI CCNNNNNNNGG 4 cut(s) 108, 221, 425, 788
BsmAI GTCTC 1 cut(s) 772
BsmFI GGGAC 1 cut(s) 711
BsnI GGCC 2 cut(s) 95, 214
Bso31I GGTCTC 1 cut(s) 772
Bsp1286I GDGCHC 2 cut(s) 21, 114
Bsp143I GATC 6 cut(s) 42, 54, 183, 332, 555, 583
Bsp19I CCATGG 1 cut(s) 551
BspACI CCGC 5 cut(s) 33, 122, 157, 189, 192
BspANI GGCC 2 cut(s) 95, 214
BspCNI CTCAG 2 cut(s) 457, 661
BspLI GGNNCC 3 cut(s) 74, 699, 700
BspOI GCTAGC 1 cut(s) 527
BspPI GGATC 2 cut(s) 563, 578
BspQI GCTCTTC 1 cut(s) 26
BspTNI GGTCTC 1 cut(s) 772
BsrDI GCAATG 1 cut(s) 258
BsrI ACTGG 3 cut(s) 112, 587, 770
BssECI CCNNGG 3 cut(s) 13, 208, 551
BssMI GATC 6 cut(s) 42, 54, 183, 332, 555, 583
BssT1I CCWWGG 2 cut(s) 13, 551
Bst4CI ACNGT 1 cut(s) 237
Bst6I CTCTTC 1 cut(s) 26
BstC8I GCNNGC 3 cut(s) 525, 566, 657
BstDEI CTNAG 3 cut(s) 439, 444, 669
BstDSI CCRYGG 2 cut(s) 208, 551
BstENI CCTNNNNNAGG 1 cut(s) 219
BstF5I GGATG 2 cut(s) 406, 580
BstKTI GATC 6 cut(s) 45, 57, 186, 335, 558, 586
BstMAI GTCTC 1 cut(s) 772
BstMBI GATC 6 cut(s) 42, 54, 183, 332, 555, 583
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 3 cut(s) 194, 524, 689
BstSCI CCNGG 1 cut(s) 773
BstV1I GCAGC 2 cut(s) 206, 527
BsuRI GGCC 2 cut(s) 95, 214
BtgI CCRYGG 2 cut(s) 208, 551
BtsCI GGATG 2 cut(s) 406, 580
BtsI GCAGTG 1 cut(s) 515
BtsIMutI CAGTG 3 cut(s) 105, 515, 594
Cac8I GCNNGC 3 cut(s) 525, 566, 657
Cfr13I GGNCC 6 cut(s) 10, 94, 212, 428, 635, 698
Csp6I GTAC 1 cut(s) 460
CviAII CATG 5 cut(s) 200, 227, 361, 552, 559
CviQI GTAC 1 cut(s) 460
DdeI CTNAG 3 cut(s) 439, 444, 669
DpnI GATC 6 cut(s) 44, 56, 185, 334, 557, 585
DpnII GATC 6 cut(s) 42, 54, 183, 332, 555, 583
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EciI GGCGGA 1 cut(s) 48
Eco130I CCWWGG 2 cut(s) 13, 551
Eco24I GRGCYC 2 cut(s) 21, 114
Eco31I GGTCTC 1 cut(s) 772
Eco47I GGWCC 4 cut(s) 10, 428, 635, 698
EcoNI CCTNNNNNAGG 1 cut(s) 219
EcoO109I RGGNCCY 2 cut(s) 635, 698
EcoT14I CCWWGG 2 cut(s) 13, 551
EcoT38I GRGCYC 2 cut(s) 21, 114
ErhI CCWWGG 2 cut(s) 13, 551
FaeI CATG 5 cut(s) 203, 230, 364, 555, 562
FaqI GGGAC 1 cut(s) 711
FatI CATG 5 cut(s) 199, 226, 360, 551, 558
FauI CCCGC 1 cut(s) 164
FauNDI CATATG 1 cut(s) 502
Fnu4HI GCNGC 4 cut(s) 189, 192, 195, 516
FokI GGATG 2 cut(s) 413, 587
FriOI GRGCYC 2 cut(s) 21, 114
Fsp4HI GCNGC 4 cut(s) 189, 192, 195, 516
FspBI CTAG 1 cut(s) 524
GluI GCNGC 4 cut(s) 189, 192, 195, 516
HaeIII GGCC 2 cut(s) 95, 214
HapII CCGG 1 cut(s) 775
Hin1II CATG 5 cut(s) 203, 230, 364, 555, 562
HinfI GANTC 3 cut(s) 173, 321, 710
HpaII CCGG 1 cut(s) 775
HphI GGTGA 3 cut(s) 106, 111, 705
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 3 cut(s) 47, 332, 447
Hpy188III TCNNGA 2 cut(s) 581, 633
Hpy8I GTNNAC 1 cut(s) 376
Hpy99I CGWCG 3 cut(s) 29, 32, 81
HpyAV CCTTC 3 cut(s) 162, 391, 603
HpyCH4III ACNGT 1 cut(s) 237
HpyCH4V TGCA 9 cut(s) 4, 251, 479, 508, 564, 603, 692, 728, 748
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 524, 689
HpyF3I CTNAG 3 cut(s) 439, 444, 669
Hsp92II CATG 5 cut(s) 203, 230, 364, 555, 562
KflI GGGWCCC 1 cut(s) 698
Kzo9I GATC 6 cut(s) 42, 54, 183, 332, 555, 583
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 4 cut(s) 78, 364, 421, 583
LpnPI CCDG 8 cut(s) 77, 93, 594, 600, 618, 669, 751, 788
Lsp1109I GCAGC 2 cut(s) 206, 527
LweI GCATC 1 cut(s) 565
MaeI CTAG 1 cut(s) 524
MaeIII GTNAC 2 cut(s) 322, 711
MalI GATC 6 cut(s) 44, 56, 185, 334, 557, 585
MboI GATC 6 cut(s) 42, 54, 183, 332, 555, 583
MboII GAAGA 3 cut(s) 13, 315, 400
MhlI GDGCHC 2 cut(s) 21, 114
MluCI AATT 2 cut(s) 287, 673
MlyI GAGTC 3 cut(s) 182, 330, 719
MmeI TCCRAC 2 cut(s) 201, 496
MseI TTAA 2 cut(s) 339, 594
MspI CCGG 1 cut(s) 775
MspR9I CCNGG 1 cut(s) 775
MwoI GCNNNNNNNGC 3 cut(s) 194, 524, 689
NciI CCSGG 1 cut(s) 775
NcoI CCATGG 1 cut(s) 551
NdeI CATATG 1 cut(s) 502
NdeII GATC 6 cut(s) 42, 54, 183, 332, 555, 583
NheI GCTAGC 1 cut(s) 523
NlaIII CATG 5 cut(s) 203, 230, 364, 555, 562
NlaIV GGNNCC 3 cut(s) 74, 699, 700
NmuCI GTSAC 2 cut(s) 322, 711
PciSI GCTCTTC 1 cut(s) 26
PflMI CCANNNNNTGG 1 cut(s) 788
PfoI TCCNGGA 1 cut(s) 773
PkrI GCNGC 4 cut(s) 190, 193, 196, 517
Ple19I CGATCG 1 cut(s) 186
PleI GAGTC 3 cut(s) 181, 329, 718
PpsI GAGTC 3 cut(s) 181, 329, 718
PpuMI RGGWCCY 2 cut(s) 635, 698
PshBI ATTAAT 1 cut(s) 594
Psp5II RGGWCCY 2 cut(s) 635, 698
PspN4I GGNNCC 3 cut(s) 74, 699, 700
PspPI GGNCC 6 cut(s) 10, 94, 212, 428, 635, 698
PspPPI RGGWCCY 2 cut(s) 635, 698
PvuI CGATCG 1 cut(s) 186
RsaI GTAC 1 cut(s) 461
RsaNI GTAC 1 cut(s) 460
SapI GCTCTTC 1 cut(s) 26
SaqAI TTAA 2 cut(s) 339, 594
SatI GCNGC 4 cut(s) 189, 192, 195, 516
Sau3AI GATC 6 cut(s) 42, 54, 183, 332, 555, 583
Sau96I GGNCC 6 cut(s) 10, 94, 212, 428, 635, 698
SchI GAGTC 3 cut(s) 182, 330, 719
ScrFI CCNGG 1 cut(s) 775
SduI GDGCHC 2 cut(s) 21, 114
SfaNI GCATC 1 cut(s) 565
SinI GGWCC 4 cut(s) 10, 428, 635, 698
SmlI CTYRAG 1 cut(s) 717
SmoI CTYRAG 1 cut(s) 717
Sse9I AATT 2 cut(s) 287, 673
SsiI CCGC 5 cut(s) 33, 122, 157, 189, 192
SspI AATATT 1 cut(s) 541
SspMI CTAG 1 cut(s) 524
StyD4I CCNGG 1 cut(s) 773
StyI CCWWGG 2 cut(s) 13, 551
TaaI ACNGT 1 cut(s) 237
TaqI TCGA 3 cut(s) 24, 57, 182
TasI AATT 2 cut(s) 287, 673
TatI WGTACW 1 cut(s) 459
TauI GCSGC 2 cut(s) 191, 194
Tru1I TTAA 2 cut(s) 339, 594
Tru9I TTAA 2 cut(s) 339, 594
TscAI CASTG 3 cut(s) 112, 515, 594
TseFI GTSAC 2 cut(s) 322, 711
TseI GCWGC 2 cut(s) 194, 515
Tsp45I GTSAC 2 cut(s) 322, 711
TspDTI ATGAA 5 cut(s) 269, 357, 401, 410, 417
TspGWI ACGGA 2 cut(s) 65, 621
TspRI CASTG 3 cut(s) 112, 515, 594
Van91I CCANNNNNTGG 1 cut(s) 788
VpaK11BI GGWCC 4 cut(s) 10, 428, 635, 698
VspI ATTAAT 1 cut(s) 594
XagI CCTNNNNNAGG 1 cut(s) 219
XapI RAATTY 1 cut(s) 287
XspI CTAG 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.