Rh3AG327000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0009994
Physical Location & Seq
Forward (+)
46435135 .. 46440141
5007 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG327000.1

Sequence Viewer

Length: 396 bp
ATGGACGAAGAAGAAGAAGCTCCAACTCATCGGCCTCCTCCTCCGTTTGTGGAGGTGAAGTGCGAGAGTTCAGGCATGAGAAGGCGCTTCGCAATGGGCACAGAAGCAGGTTTCGCAGTGTCAGTCATAAACAGCAAATTGGGTACAGTGGGGGACCCTCACCCTCACCCTCTTTCTCTGCACATTAAAGCCGTTAAGGAAGGTGAGGAGCCTATCACTTTCGGACCCAACTCACTTCTCGTCGACTACGGCCCCGGTTGGACGTTACACACTGTTACCCAACTCCCATTTTCTGGTAATTCTACTTCTGTTTGGTTTTGTCAACACTTGTTCAATGTTGGATTTTGTCGTTATTTTTTGATTTTGTTCAACTATAGGTGGAGAAGGTGTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFT

Protein Analysis

131

Amino Acids

14.81

Weight (kDa)

6.58

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 98
AccB7I CCANNNNNTGG 1 cut(s) 293
AccI GTMKAC 1 cut(s) 243
AfaI GTAC 1 cut(s) 145
AfiI CCNNNNNNNGG 1 cut(s) 293
AgsI TTSAA 2 cut(s) 334, 370
AluBI AGCT 1 cut(s) 20
AluI AGCT 1 cut(s) 20
AoxI GGCC 2 cut(s) 32, 250
AspLEI GCGC 1 cut(s) 87
AspS9I GGNCC 3 cut(s) 154, 224, 251
AsuC2I CCSGG 1 cut(s) 255
AsuHPI GGTGA 4 cut(s) 67, 152, 158, 215
AvaII GGWCC 2 cut(s) 154, 224
BaeGI GKGCMC 1 cut(s) 101
BarI GAAGNNNNNNTAC 2 cut(s) 289, 321
BceAI ACGGC 2 cut(s) 176, 265
BcnI CCSGG 1 cut(s) 255
BfmI CTRYAG 1 cut(s) 373
BfoI RGCGCY 1 cut(s) 88
BfuAI ACCTGC 1 cut(s) 98
Bme1390I CCNGG 1 cut(s) 255
Bme18I GGWCC 2 cut(s) 154, 224
BmgT120I GGNCC 3 cut(s) 154, 224, 251
BmiI GGNNCC 5 cut(s) 155, 156, 210, 226, 253
BmrFI CCNGG 1 cut(s) 255
BpuMI CCSGG 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 253
BsaXI ACNNNNNCTCC 3 cut(s) 44, 74, 373
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse3DI GCAATG 1 cut(s) 99
BseDI CCNNGG 1 cut(s) 253
BseLI CCNNNNNNNGG 1 cut(s) 293
BseMI GCAATG 1 cut(s) 99
BseRI GAGGAG 3 cut(s) 27, 30, 221
BseSI GKGCMC 1 cut(s) 101
BsgI GTGCAG 1 cut(s) 164
BshFI GGCC 2 cut(s) 34, 252
BsiSI CCGG 1 cut(s) 255
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 167
BsnI GGCC 2 cut(s) 34, 252
Bsp1286I GDGCHC 1 cut(s) 101
BspANI GGCC 2 cut(s) 34, 252
BspLI GGNNCC 5 cut(s) 155, 156, 210, 226, 253
BspMI ACCTGC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 99
BssECI CCNNGG 1 cut(s) 253
Bst4CI ACNGT 2 cut(s) 148, 274
BstH2I RGCGCY 1 cut(s) 88
BstHHI GCGC 1 cut(s) 87
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 253
BstSFI CTRYAG 1 cut(s) 373
BstSLI GKGCMC 1 cut(s) 101
BsuRI GGCC 2 cut(s) 34, 252
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 3 cut(s) 123, 153, 270
BveI ACCTGC 1 cut(s) 98
CfoI GCGC 1 cut(s) 87
Cfr13I GGNCC 3 cut(s) 154, 224, 251
Csp6I GTAC 1 cut(s) 144
CviAII CATG 1 cut(s) 76
CviJI RGCY 5 cut(s) 20, 34, 191, 211, 252
CviKI_1 RGCY 5 cut(s) 20, 34, 191, 211, 252
CviQI GTAC 1 cut(s) 144
Eco47I GGWCC 2 cut(s) 154, 224
EcoO109I RGGNCCY 1 cut(s) 154
FaeI CATG 1 cut(s) 79
FaiI YATR 3 cut(s) 77, 128, 375
FaqI GGGAC 1 cut(s) 167
FatI CATG 1 cut(s) 75
FblI GTMKAC 1 cut(s) 243
GlaI GCGC 1 cut(s) 86
HaeII RGCGCY 1 cut(s) 88
HaeIII GGCC 2 cut(s) 34, 252
HapII CCGG 1 cut(s) 255
HhaI GCGC 1 cut(s) 87
Hin1II CATG 1 cut(s) 79
Hin6I GCGC 1 cut(s) 85
HinP1I GCGC 1 cut(s) 85
HincII GTYRAC 2 cut(s) 244, 323
HindII GTYRAC 2 cut(s) 244, 323
HpaII CCGG 1 cut(s) 255
HphI GGTGA 4 cut(s) 67, 152, 158, 215
Hpy166II GTNNAC 2 cut(s) 244, 323
Hpy188I TCNGA 1 cut(s) 224
Hpy8I GTNNAC 2 cut(s) 244, 323
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 3 cut(s) 75, 194, 378
HpyCH4III ACNGT 2 cut(s) 148, 274
HpyCH4IV ACGT 1 cut(s) 263
HpyCH4V TGCA 1 cut(s) 181
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpySE526I ACGT 1 cut(s) 263
Hsp92II CATG 1 cut(s) 79
HspAI GCGC 1 cut(s) 85
KflI GGGWCCC 1 cut(s) 154
LmnI GCTCC 2 cut(s) 25, 208
LpnPI CCDG 4 cut(s) 57, 93, 268, 279
MaeII ACGT 1 cut(s) 263
MaeIII GTNAC 2 cut(s) 264, 274
MboII GAAGA 3 cut(s) 20, 23, 26
MhlI GDGCHC 1 cut(s) 101
MluCI AATT 2 cut(s) 137, 298
MmeI TCCRAC 3 cut(s) 47, 239, 319
MnlI CCTC 8 cut(s) 45, 46, 48, 51, 168, 174, 180, 199
MseI TTAA 2 cut(s) 186, 195
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 1 cut(s) 255
MwoI GCNNNNNNNGC 1 cut(s) 113
NciI CCSGG 1 cut(s) 255
NlaIII CATG 1 cut(s) 79
NlaIV GGNNCC 5 cut(s) 155, 156, 210, 226, 253
PflMI CCANNNNNTGG 1 cut(s) 293
PpuMI RGGWCCY 1 cut(s) 154
Psp5II RGGWCCY 1 cut(s) 154
PspN4I GGNNCC 5 cut(s) 155, 156, 210, 226, 253
PspPI GGNCC 3 cut(s) 154, 224, 251
PspPPI RGGWCCY 1 cut(s) 154
RsaI GTAC 1 cut(s) 145
RsaNI GTAC 1 cut(s) 144
SalI GTCGAC 1 cut(s) 242
SaqAI TTAA 2 cut(s) 186, 195
Sau96I GGNCC 3 cut(s) 154, 224, 251
ScrFI CCNGG 1 cut(s) 255
SduI GDGCHC 1 cut(s) 101
SetI ASST 7 cut(s) 22, 57, 112, 205, 266, 380, 389
SfcI CTRYAG 1 cut(s) 373
SgeI CNNG 9 cut(s) 76, 84, 88, 120, 251, 266, 267, 306, 340
SinI GGWCC 2 cut(s) 154, 224
Sse9I AATT 2 cut(s) 137, 298
StyD4I CCNGG 1 cut(s) 253
TaaI ACNGT 2 cut(s) 148, 274
TaiI ACGT 1 cut(s) 266
TaqI TCGA 1 cut(s) 243
TasI AATT 2 cut(s) 137, 298
Tru1I TTAA 2 cut(s) 186, 195
Tru9I TTAA 2 cut(s) 186, 195
TscAI CASTG 3 cut(s) 123, 153, 277
TspGWI ACGGA 1 cut(s) 33
TspRI CASTG 3 cut(s) 123, 153, 277
Van91I CCANNNNNTGG 1 cut(s) 293
VpaK11BI GGWCC 2 cut(s) 154, 224
XmiI GTMKAC 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.