Rh4BG143500

aminoacylase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003926
Physical Location & Seq
Forward (+)
25518072 .. 25523227
5156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG143500.1

Sequence Viewer

Length: 1026 bp
ATGGCCTCCGGTCAACATTCACAAATAAGAAATGGGGTTGAAAACTTGGATCAACATTGTGCTGCTACATGCAGGTGGATAAGTGTCGTCTTGAGTATAATGAGGATGTCCGGGTTCAAGTTGAAATGGTTGGACCAAGTTTTGCCACTACACGAAAGAAATGTGCCCGGTGAATGGGATGGCTATGGAGCAGACTTCACAACCGAAGGGAACCCGATTGAACTCCCAGAAAGGGTTGTGCCTGATGCTTACAGGGAGTGGGAGGTTAAGGTTTTCGACTGGCAGACTCAATGTCCCACTCTTGCTGACCCAGAAGACCAAGTTCTTGCCTATAAGACGATAGAGCTACTTCCCACAGTTGGATGTGAAGCCGATGCTGCTACACGGTACAGCATTCTTGAGAGGAATGTGGGTGGTGTGAACAACAAAGTTTCTGCCTTTGCGTATCAGTCTAGTGGATGTTATGTGTCTGTTTTGCCAGTGGAGGATAAGAGTTTGAATAAGTTGTTGGAAGTAGAGTATTGCTTGATCAATCCTCAAGATAAGGAGTCTCGTGTGAGGATAATTCAGGTGATTCACATTGACAATATGAAGATGGTATTGCAGAGTCTTAGAGTTCTTTGTGAACAGTGGTATGGGCCATTCAGAAATGGGGAGCACTTAGGTGGATGTGCCATCCGTGATTCTGCTTTTTCTTCTACGCCCGCATTGGAAGCTTCAAAAGTTGTTGGTACTTGGCGGAGCTCCAGTGCTGTTGCCACTTTTAATGGTTCTCAAAATGACTTCATCGAGCTCTCTCACAGAGGCTACTACAACAATGTTAAATTCCACAGAATCATCAAGGAAGAGCTCAGAGCTCGTAATCTTTTCTATGCTCTTTGGGTGCCTAATCTATTCATGAAAAGGGTTGGAAATAATGAAAAGTGGTCACTCTTTTGTCCTAATAGTGTCCAGGTCAGGGAGATTGCTGGAATGAAAGCTTCGAACAGCTTTGCACTCGTTATGAAAATCATGTTGTTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

341

Amino Acids

38.72

Weight (kDa)

6.67

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuc_red_lgC PF02867 265 - 328 1.5e-11 Ribonucleotide reductase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 63
Acc36I ACCTGC 1 cut(s) 63
AccB1I GGYRCC 1 cut(s) 883
AciI CCGC 2 cut(s) 705, 739
AclWI GGATC 1 cut(s) 57
AcsI RAATTY 1 cut(s) 824
AfaI GTAC 2 cut(s) 389, 733
AfiI CCNNNNNNNGG 4 cut(s) 174, 232, 359, 958
AgsI TTSAA 7 cut(s) 41, 118, 124, 221, 499, 720, 1021
AhdI GACNNNNNGTC 1 cut(s) 291
AjnI CCWGG 1 cut(s) 951
AjuI GAANNNNNNNTTGG 2 cut(s) 491, 523
AleI CACNNNNGTG 1 cut(s) 663
AluBI AGCT 8 cut(s) 346, 716, 744, 793, 850, 857, 980, 990
AluI AGCT 8 cut(s) 346, 716, 744, 793, 850, 857, 980, 990
Alw21I GWGCWC 5 cut(s) 660, 746, 795, 852, 859
Alw26I GTCTC 1 cut(s) 555
AlwI GGATC 1 cut(s) 57
AoxI GGCC 2 cut(s) 3, 638
ApeKI GCWGC 2 cut(s) 62, 377
ApoI RAATTY 1 cut(s) 824
AspS9I GGNCC 2 cut(s) 133, 638
AsuC2I CCSGG 2 cut(s) 112, 168
AsuHPI GGTGA 2 cut(s) 182, 583
AsuII TTCGAA 1 cut(s) 983
AvaII GGWCC 1 cut(s) 133
BaeGI GKGCMC 1 cut(s) 168
BanI GGYRCC 1 cut(s) 883
BanII GRGCYC 4 cut(s) 746, 795, 852, 859
BauI CACGAG 1 cut(s) 552
BbsI GAAGAC 1 cut(s) 321
Bbv12I GWGCWC 5 cut(s) 660, 746, 795, 852, 859
BbvI GCAGC 2 cut(s) 49, 364
BccI CCATC 3 cut(s) 173, 589, 683
BciT130I CCWGG 1 cut(s) 953
BclI TGATCA 1 cut(s) 528
BcnI CCSGG 2 cut(s) 112, 168
BcoDI GTCTC 1 cut(s) 555
BfaI CTAG 1 cut(s) 453
BfuAI ACCTGC 1 cut(s) 63
BisI GCNGC 2 cut(s) 63, 378
BlsI GCNGC 2 cut(s) 64, 379
Bme1390I CCNGG 3 cut(s) 112, 168, 953
Bme18I GGWCC 1 cut(s) 133
BmeRI GACNNNNNGTC 1 cut(s) 291
BmgT120I GGNCC 2 cut(s) 133, 638
BmiI GGNNCC 2 cut(s) 212, 885
BmrFI CCNGG 3 cut(s) 112, 168, 953
BmsI GCATC 2 cut(s) 235, 364
BpiI GAAGAC 1 cut(s) 321
BpmI CTGGAG 1 cut(s) 730
Bpu14I TTCGAA 1 cut(s) 983
BpuEI CTTGAG 3 cut(s) 112, 419, 522
BpuMI CCSGG 2 cut(s) 112, 168
BsaWI WCCGGW 1 cut(s) 8
BsaXI ACNNNNNCTCC 2 cut(s) 733, 763
Bsc4I CCNNNNNNNGG 4 cut(s) 174, 232, 359, 958
Bse1I ACTGG 3 cut(s) 284, 479, 747
BseBI CCWGG 1 cut(s) 953
BseGI GGATG 6 cut(s) 111, 184, 368, 464, 674, 675
BseLI CCNNNNNNNGG 4 cut(s) 174, 232, 359, 958
BseMII CTCAG 1 cut(s) 865
BseNI ACTGG 3 cut(s) 284, 479, 747
BseSI GKGCMC 1 cut(s) 168
BseXI GCAGC 2 cut(s) 49, 364
BshFI GGCC 2 cut(s) 5, 640
BshNI GGYRCC 1 cut(s) 883
BsiHKAI GWGCWC 5 cut(s) 660, 746, 795, 852, 859
BsiSI CCGG 3 cut(s) 9, 111, 168
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 4 cut(s) 174, 232, 359, 958
BsmAI GTCTC 1 cut(s) 555
BsmFI GGGAC 1 cut(s) 279
BsmI GAATGC 1 cut(s) 393
BsnI GGCC 2 cut(s) 5, 640
Bsp119I TTCGAA 1 cut(s) 983
Bsp1286I GDGCHC 6 cut(s) 168, 660, 746, 795, 852, 859
Bsp143I GATC 2 cut(s) 49, 528
BspACI CCGC 2 cut(s) 705, 739
BspANI GGCC 2 cut(s) 5, 640
BspCNI CTCAG 1 cut(s) 864
BspHI TCATGA 1 cut(s) 897
BspLI GGNNCC 2 cut(s) 212, 885
BspMI ACCTGC 1 cut(s) 63
BspPI GGATC 1 cut(s) 57
BspQI GCTCTTC 1 cut(s) 840
BspT104I TTCGAA 1 cut(s) 983
BspT107I GGYRCC 1 cut(s) 883
BsrI ACTGG 3 cut(s) 284, 479, 747
BssMI GATC 2 cut(s) 49, 528
BssSI CACGAG 1 cut(s) 552
Bst2BI CACGAG 1 cut(s) 552
Bst2UI CCWGG 1 cut(s) 953
Bst4CI ACNGT 3 cut(s) 358, 387, 630
Bst6I CTCTTC 1 cut(s) 840
BstBI TTCGAA 1 cut(s) 983
BstC8I GCNNGC 1 cut(s) 705
BstDEI CTNAG 3 cut(s) 611, 661, 851
BstF5I GGATG 6 cut(s) 111, 184, 368, 464, 674, 675
BstKTI GATC 2 cut(s) 52, 531
BstMAI GTCTC 1 cut(s) 555
BstMBI GATC 2 cut(s) 49, 528
BstMWI GCNNNNNNNGC 2 cut(s) 377, 713
BstNI CCWGG 1 cut(s) 953
BstNSI RCATGY 1 cut(s) 72
BstSCI CCNGG 3 cut(s) 110, 166, 951
BstSLI GKGCMC 1 cut(s) 168
BstV1I GCAGC 2 cut(s) 49, 364
BstV2I GAAGAC 1 cut(s) 321
BsuRI GGCC 2 cut(s) 5, 640
BtsCI GGATG 6 cut(s) 111, 184, 368, 464, 674, 675
BtsIMutI CAGTG 3 cut(s) 486, 635, 754
BveI ACCTGC 1 cut(s) 63
Cac8I GCNNGC 1 cut(s) 705
CciI TCATGA 1 cut(s) 897
Cfr13I GGNCC 2 cut(s) 133, 638
Csp6I GTAC 2 cut(s) 388, 732
CviAII CATG 3 cut(s) 69, 898, 1012
CviQI GTAC 2 cut(s) 388, 732
DdeI CTNAG 3 cut(s) 611, 661, 851
DpnI GATC 2 cut(s) 51, 530
DpnII GATC 2 cut(s) 49, 528
DriI GACNNNNNGTC 1 cut(s) 291
Eam1104I CTCTTC 1 cut(s) 840
Eam1105I GACNNNNNGTC 1 cut(s) 291
EarI CTCTTC 1 cut(s) 840
EciI GGCGGA 1 cut(s) 754
Ecl136II GAGCTC 4 cut(s) 744, 793, 850, 857
Eco24I GRGCYC 4 cut(s) 746, 795, 852, 859
Eco47I GGWCC 1 cut(s) 133
Eco53kI GAGCTC 4 cut(s) 744, 793, 850, 857
EcoICRI GAGCTC 4 cut(s) 744, 793, 850, 857
EcoRII CCWGG 1 cut(s) 951
EcoT38I GRGCYC 4 cut(s) 746, 795, 852, 859
FaeI CATG 3 cut(s) 72, 901, 1015
FaqI GGGAC 1 cut(s) 279
FatI CATG 3 cut(s) 68, 897, 1011
FauI CCCGC 1 cut(s) 712
FbaI TGATCA 1 cut(s) 528
Fnu4HI GCNGC 2 cut(s) 63, 378
FokI GGATG 6 cut(s) 118, 191, 375, 471, 662, 681
FriOI GRGCYC 4 cut(s) 746, 795, 852, 859
Fsp4HI GCNGC 2 cut(s) 63, 378
FspBI CTAG 1 cut(s) 453
GluI GCNGC 2 cut(s) 63, 378
GsuI CTGGAG 1 cut(s) 730
HaeIII GGCC 2 cut(s) 5, 640
HapII CCGG 3 cut(s) 9, 111, 168
Hin1II CATG 3 cut(s) 72, 901, 1015
HincII GTYRAC 1 cut(s) 14
HindII GTYRAC 1 cut(s) 14
HindIII AAGCTT 2 cut(s) 714, 978
HinfI GANTC 6 cut(s) 286, 548, 574, 607, 683, 834
HpaII CCGG 3 cut(s) 9, 111, 168
HphI GGTGA 2 cut(s) 182, 583
Hpy166II GTNNAC 3 cut(s) 14, 421, 626
Hpy188I TCNGA 2 cut(s) 647, 854
Hpy188III TCNNGA 4 cut(s) 91, 398, 539, 898
Hpy8I GTNNAC 3 cut(s) 14, 421, 626
HpyAV CCTTC 1 cut(s) 200
HpyCH4III ACNGT 3 cut(s) 358, 387, 630
HpyCH4V TGCA 3 cut(s) 72, 604, 995
HpyF10VI GCNNNNNNNGC 2 cut(s) 377, 713
HpyF3I CTNAG 3 cut(s) 611, 661, 851
Hsp92II CATG 3 cut(s) 72, 901, 1015
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 2 cut(s) 49, 528
LguI GCTCTTC 1 cut(s) 840
LmnI GCTCC 4 cut(s) 188, 655, 741, 749
Lsp1109I GCAGC 2 cut(s) 49, 364
LweI GCATC 2 cut(s) 235, 364
MaeI CTAG 1 cut(s) 453
MaeIII GTNAC 1 cut(s) 927
MalI GATC 2 cut(s) 51, 530
MboI GATC 2 cut(s) 49, 528
MboII GAAGA 4 cut(s) 326, 604, 687, 857
MhlI GDGCHC 6 cut(s) 168, 660, 746, 795, 852, 859
MluCI AATT 2 cut(s) 564, 824
MlyI GAGTC 3 cut(s) 280, 557, 616
MmeI TCCRAC 4 cut(s) 111, 340, 489, 889
MnlI CCTC 8 cut(s) 16, 96, 256, 396, 478, 546, 552, 797
MseI TTAA 3 cut(s) 267, 765, 822
MslI CAYNNNNRTG 2 cut(s) 73, 663
MspI CCGG 3 cut(s) 9, 111, 168
MspR9I CCNGG 3 cut(s) 112, 168, 953
Mva1269I GAATGC 1 cut(s) 393
MvaI CCWGG 1 cut(s) 953
MwoI GCNNNNNNNGC 2 cut(s) 377, 713
NciI CCSGG 2 cut(s) 112, 168
NdeII GATC 2 cut(s) 49, 528
NlaIII CATG 3 cut(s) 72, 901, 1015
NlaIV GGNNCC 2 cut(s) 212, 885
NmuCI GTSAC 1 cut(s) 927
NspI RCATGY 1 cut(s) 72
NspV TTCGAA 1 cut(s) 983
OliI CACNNNNGTG 1 cut(s) 663
PagI TCATGA 1 cut(s) 897
PaqCI CACCTGC 1 cut(s) 63
PciSI GCTCTTC 1 cut(s) 840
PctI GAATGC 1 cut(s) 393
PfeI GAWTC 3 cut(s) 574, 683, 834
PkrI GCNGC 2 cut(s) 64, 379
PleI GAGTC 3 cut(s) 280, 556, 615
PpsI GAGTC 3 cut(s) 280, 556, 615
Psp124BI GAGCTC 4 cut(s) 746, 795, 852, 859
Psp6I CCWGG 1 cut(s) 951
PspGI CCWGG 1 cut(s) 951
PspN4I GGNNCC 2 cut(s) 212, 885
PspPI GGNCC 2 cut(s) 133, 638
RsaI GTAC 2 cut(s) 389, 733
RsaNI GTAC 2 cut(s) 388, 732
RseI CAYNNNNRTG 2 cut(s) 73, 663
SacI GAGCTC 4 cut(s) 746, 795, 852, 859
SapI GCTCTTC 1 cut(s) 840
SaqAI TTAA 3 cut(s) 267, 765, 822
SatI GCNGC 2 cut(s) 63, 378
Sau3AI GATC 2 cut(s) 49, 528
Sau96I GGNCC 2 cut(s) 133, 638
SchI GAGTC 3 cut(s) 280, 557, 616
ScrFI CCNGG 3 cut(s) 112, 168, 953
SduI GDGCHC 6 cut(s) 168, 660, 746, 795, 852, 859
SfaNI GCATC 2 cut(s) 235, 364
SfuI TTCGAA 1 cut(s) 983
SinI GGWCC 1 cut(s) 133
SmiMI CAYNNNNRTG 2 cut(s) 73, 663
SmlI CTYRAG 3 cut(s) 91, 398, 537
SmoI CTYRAG 3 cut(s) 91, 398, 537
Sse9I AATT 2 cut(s) 564, 824
SsiI CCGC 2 cut(s) 705, 739
SspMI CTAG 1 cut(s) 453
SstI GAGCTC 4 cut(s) 746, 795, 852, 859
StyD4I CCNGG 3 cut(s) 110, 166, 951
TaaI ACNGT 3 cut(s) 358, 387, 630
TaqI TCGA 3 cut(s) 276, 789, 983
TasI AATT 2 cut(s) 564, 824
TfiI GAWTC 3 cut(s) 574, 683, 834
Tru1I TTAA 3 cut(s) 267, 765, 822
Tru9I TTAA 3 cut(s) 267, 765, 822
TscAI CASTG 3 cut(s) 486, 635, 754
TseFI GTSAC 1 cut(s) 927
TseI GCWGC 2 cut(s) 62, 377
Tsp45I GTSAC 1 cut(s) 927
TspDTI ATGAA 7 cut(s) 605, 775, 886, 914, 933, 989, 1019
TspGWI ACGGA 1 cut(s) 668
TspRI CASTG 3 cut(s) 486, 635, 754
VpaK11BI GGWCC 1 cut(s) 133
XapI RAATTY 1 cut(s) 824
XceI RCATGY 1 cut(s) 72
XspI CTAG 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.